Article
Engineering, Chemical
Rashi Vishwakarma, Anushree Malik
Summary: A green extraction process is proposed to reduce energy and petroleum solvent consumption, while maintaining environmental safety and quality. The study focuses on generating a highly permeable cell using crude fungal enzymes, enabling simultaneous cultivation and extraction. A novel pretreatment method combining partial enzymatic digestion and maintaining viability of microalgae cells allows for further product recovery from the same biomass.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Review
Energy & Fuels
Farhana Bibi, Asif Jamal, Zaixing Huang, Michael Urynowicz, Muhammad Ishtiaq Ali
Summary: Microalgae have been identified as a promising alternative feedstock for carbon-neutral biofuel production. This study aims to explore the effects of various abiotic stresses on microalgal biofuel production and review strategies to induce lipid production in microalgae. The manipulation of abiotic growth factors has been shown to facilitate high lipid and biomass accumulation in microalgae cultivation. The commercialization of algae-based biofuels through cost-effective and multi-product generation routes remains valid in the context of global decarbonization movement.
Article
Food Science & Technology
Die Zhao, Chunji Li
Summary: Low temperature promotes exopolysaccharide synthesis in R. glutinis ZHK, while high temperature enhances the yield of certain carotenoids and total lipids.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Engineering, Environmental
Chetan Paliwal, Pannaga Pavan Jutur
Summary: Chemical molecules like CBHA, CPG, and CACH have shown to enhance cell growth and lipid productivities in microalgae at nanomolar concentrations. They target specific pathways in the microalgae to produce unique metabolites, offering a non-gene disruptive strategy for sustainable algal biorefineries.
CHEMICAL ENGINEERING JOURNAL
(2021)
Review
Biology
Yevhen Maltsev, Kateryna Maltseva, Maxim Kulikovskiy, Svetlana Maltseva
Summary: Microalgae are a valuable natural resource for producing a variety of value-added products. The optimal light intensity for their growth is around 26,400 µmol photons m(-2) s(-1), with higher intensity leading to increased lipid synthesis and triacylglyceride content. Light intensity also affects the synthesis of fatty acids and carotenoids in microalgae biomass. Additionally, combining light with other stresses can enhance the productivity of algae.
Article
Chemistry, Multidisciplinary
Kushi Yadav, Shashi Kumar, Ganesh Chandrakant Nikalje, Monika Prakash Rai
Summary: This study aimed to enhance the synthesis of carotenoids in microalgae by manipulating abiotic factors without affecting their growth. The optimized conditions resulted in increased biomass, lipid accumulation, and carotenoid content compared to the control conditions.
APPLIED SCIENCES-BASEL
(2023)
Article
Chemistry, Medicinal
Eleonora Montuori, Maria Saggiomo, Chiara Lauritano
Summary: Cold environments, including deep ocean, alpine, and polar areas, host various species that have adapted to survive in harsh conditions. Microalgae, as abundant microbial communities, have developed stress-responsive strategies to live in low light, low temperature, and ice coverage conditions. These species exhibit bioactivities, such as antioxidant and anticancer activities, that can be potentially exploited for human applications. This review summarizes these bioactivities and discusses the possibility of eco-sustainable exploitation of cold-adapted microalgae through controlled photobioreactors.
Article
Cell Biology
Rabinder Singh, Asha Arumugam Nesamma, Alka Narula, Pannaga Pavan Jutur
Summary: The present study focused on enhancing the total tocopherol yields in Monoraphidium sp. CABeR41 by manipulating carbon supplementation and nitrate concentrations. The results showed that carbon supplementation with varying nitrate concentrations significantly increased the total tocopherol productivity. Additionally, metabolomics analysis revealed the molecular mechanisms underlying tocopherol accumulation, including upregulation of nitrogen assimilation and the tricarboxylic acid cycle. This study highlights the potential of Monoraphidium sp. as a valuable strain for producing biomass, tocopherols, and other biocommodities.
Article
Agricultural Engineering
Anil Kumar Patel, Akash Pralhad Vadrale, Yi-Sheng Tseng, Chiu-Wen Chen, Cheng-Di Dong, Reeta Rani Singhania
Summary: A bioprospecting study was conducted in Taiwan to isolate a potential lutein-producing strain and optimize the cultivation and extraction conditions. The chosen strain, Chlorella sorokiniana Kh12, showed high yields of lutein and lipid production. The results suggest that this strain has potential for commercial production of lutein and lipid.
BIORESOURCE TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Xiaolan Huang, Fang Li, Yi Wang, Xinbei Jia, Nan Jia, Fei Xiao, Chunrong Sun, Jin Fu, Min Chen, Xiaodai Cui, Dong Qu, Laurence Don Wai Luu, Jun Tai, Jieqiong Li
Summary: Metabolomics was used to identify potential biomarkers for RSV pneumonia and multi-omics analysis revealed the pathogenies of RSV infection.
Article
Environmental Sciences
Daniela Gomez-Martinez, Johanna Bengtson, Anders K. Nilsson, Adrian K. Clarke, Rolf Henrik Nilsson, Erik Kristiansson, Natalia Corcoll
Summary: Herbicide pollution is a global threat to plants and freshwater ecosystems. This study investigates how the green microalgal species Raphidocelis subcapitata adapts to the herbicide diflufenican and the associated trade-off expenses. The algae were exposed to diflufenican for 12 weeks and the results showed an initial dose-dependent stress phase, followed by a recovery phase. After acclimation, R. subcapitata exhibited similar growth, pigment composition, and photosynthetic performance as the unexposed algae, but with smaller cell size and changes in gene expression.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Microbiology
Martin Szotkowski, Jiri Holub, Samuel Simansky, Klara Hubacova, Pavlina Sikorova, Veronika Marinicova, Andrea Nemcova, Ivana Marova
Summary: The co-cultivation of red yeasts and microalgae allows for natural transport of gases, enhancing growth and lipid production. Pilot experiments have shown that selected strains of red yeasts and Desmodesmus sp. microalgae can thrive together, resulting in efficient biomass growth and lipid metabolite yields.
Article
Agricultural Engineering
Renata Nunes Pereira, Debora Pez Jaeschke, Ligia Damasceno Ferreira Marczak, Rosane Rech, Giovana Domeneghini Mercali
Summary: This study evaluated the effects of ultrasound on the growth of Pseudoneochloris marina and Chlorella zofingiensis. The results showed that ultrasound treatment did not increase cell proliferation of P. marina, but reduced cell density, indicating possible cell damage. On the other hand, ultrasound treatment for C. zofingiensis resulted in a 65% increase in biomass concentration compared to the control. Overall, the study demonstrates the potential of ultrasound to enhance microalgae cell proliferation.
BIORESOURCE TECHNOLOGY
(2023)
Article
Food Science & Technology
Julia Bazarnova, Liudmila Nilova, Elena Trukhina, Maya Bernavskaya, Yulia Smyatskaya, Tugba Aktar
Summary: This article discusses the use of Chlorella sorokiniana biomass in pasta recipes to enhance the product with biologically active phytonutrients and achieve desired color without synthetic dyes. Adding 5% of Chlorella biomass to pasta results in increased protein and lipid content, enriching the nutritional value of the final product.
Review
Biotechnology & Applied Microbiology
Ranjna Sirohi, Jaemin Joun, Hong Ii Choi, Vivek Kumar Gaur, Sang Jun Sim
Summary: This article discusses the potential of microalgae as an energy feedstock and the efforts to increase microalgae productivity using various omics techniques, with a particular focus on the application of genome sequencing and metabolic engineering in this field.
MICROBIAL CELL FACTORIES
(2021)
Article
Biotechnology & Applied Microbiology
Xiao-Man Sun, Zi-Xu Zhang, Ling-Ru Wang, Jing-Gang Wang, Yan Liang, Hai-Feng Yang, Rong-Sheng Tao, Yu Jiang, Jun-Jie Yang, Sheng Yang
Summary: Escherichia coli BL21 (DE3) is widely used for recombinant protein production, but overexpression of certain proteins can induce severe cell autolysis. By modifying the promoter lac to create BL21 (DE3-lac1G), a strain resistant to autolysis was developed, resulting in significantly increased enzyme activity and improved protein expression rates. This engineered strain has great potential for industrial applications.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Review
Biotechnology & Applied Microbiology
Fei Du, Yu-Zhou Wang, Ying-Shuang Xu, Tian-Qiong Shi, Wen-Zheng Liu, Xiao-Man Sun, He Huang
Summary: Thraustochytrids, as fungus-like protists, have drawn increasing attention due to their high lipid content and rapid growth rates. They have unique pathways for high DHA yield and can serve as industrial strains for DHA production, although improving strain performance alone is not sufficient for industrialization.
BIOTECHNOLOGY ADVANCES
(2021)
Review
Agriculture, Multidisciplinary
Zi-Jia Li, Yu-Zhou Wang, Ling-Ru Wang, Tian-Qiong Shi, Xiao-Man Sun, He Huang
Summary: Microorganisms, especially oleaginous yeasts like Yarrowia lipolytica, are being explored as promising sources for terpenoid production due to their high lipid content. Various metabolic engineering strategies have been developed to enhance terpenoid biosynthesis in Y. lipolytica, including the development of an orthogonal pathway, improving enzyme efficiency, and modifying precursor supply.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Qi Guo, Tian-Qiong Shi, Qian-Qian Peng, Xiao-Man Sun, Xiao-Jun Ji, He Huang
Summary: The study successfully developed Yarrowia lipolytica as a cell factory for producing the sesquiterpene alpha-humulene with significantly increased yield. This provides valuable reference for highly sustainable production of terpenoids through peroxisome engineering in Y. lipolytica.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemical Research Methods
Zi-Jia Li, Zi-Xu Zhang, Yan Xu, Tian-Qiong Shi, Chao Ye, Xiao-Man Sun, He Huang
Summary: Constructing BL21(DE3)-derived variant hosts with different T7RNAP translation levels by changing the RBS sequences can improve the production of difficult-to-express proteins.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biochemical Research Methods
Kaifeng Wang, Tian-Qiong Shi, Jinpeng Wang, Ping Wei, Rodrigo Ledesma-Amaro, Xiao-Jun Ji
Summary: A sustainable strategy for high oleic oil production was established by reprogramming the metabolism of the oleaginous yeast Yarrowia lipolytica. The engineered strain produced a high titer of high oleic microbial oil with a remarkable improvement in oil titer and oleic acid proportion compared to the starting strain.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Qi Guo, Ya-Wen Li, Fang Yan, Ke Li, Yue-Tong Wang, Chao Ye, Tian-Qiong Shi, He Huang
Summary: In this study, a robust cell factory for sustainable alpha-humulene production was engineered using Yarrowia lipolytica. Through metabolic engineering strategies, the production of alpha-humulene was significantly improved.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Review
Agriculture, Multidisciplinary
Wenjuan Li, Liuwei Cui, Jie Mai, Tian-Qiong Shi, Lu Lin, Zhi-Gang Zhang, Rodrigo Ledesma-Amaro, Weiliang Dong, Xiao-Jun Ji
Summary: This review summarizes the important roles and engineering strategies of Saccharomyces cerevisiae and Yarrowia lipolytica in terpene production, discusses the future prospects and challenges, and emphasizes guidelines for future studies.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Review
Biochemical Research Methods
Kaifeng Wang, Tian-Qiong Shi, Lu Lin, Ping Wei, Rodrigo Ledesma-Amaro, Xiao-Jun Ji
Summary: Microbial production of value-added chemicals derived from fatty acids is a sustainable alternative to petroleum-derived chemicals. Tailoring fatty acid chain length using metabolic engineering in Yarrowia lipolytica can lead to the production of tailored chain-length fatty acids and their derivatives.
ACS SYNTHETIC BIOLOGY
(2022)
Review
Biochemical Research Methods
Ya -Wen Li, Qi Guo, Qian-Qian Peng, Qi Shen, Zhi-Kui Nie, Chao Ye, Tian-Qiong Shi
Summary: Arachidonic acid is a significant fatty acid that plays an essential role in cardiovascular health and neurological development. Traditionally obtained from deep-sea fish oil, this source is limited by season and the depletion of global fish stocks. With advancements in synthetic biology, oleaginous fungi have emerged as promising microbial sources for large-scale production of arachidonic acid. This review summarizes recent progress in bioengineering oleaginous fungi for arachidonic acid production and proposes future engineering approaches to improve yield and productivity.
ACS SYNTHETIC BIOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Danshan Zhao, Yawen Li, Zhidong Zhang, Tian Xu, Chao Ye, Tianqiong Shi, Yuetong Wang
Summary: Spores and pollens are reproductive cells of seed plants and asexually reproducing sporophytes, with a natural core-shell structure and exquisite surface morphology. They have remarkable dimensional homogeneity, porosity, amphiphilicity, and adhesion. The sporopollenin exine layer provides them with chemical stability, UV resistance, and biocompatibility, and their surface groups allow easy functionalization. Spores and pollens have found wide-ranging applications in drug delivery, biological imaging, food science, microrobotics, environmental purification, flexible electronics, cell scaffolds, 3D printing materials, and biological detection. This review summarizes their structural composition, physicochemical properties, extraction and processing methods, and recent research on their applications in various fields. It also discusses the existing challenges in spores and pollen research and provides future prospects.
MATERIALS HORIZONS
(2023)
Article
Agriculture, Multidisciplinary
Qian-Qian Peng, Qi Guo, Cheng Chen, Ping Song, Yue-Tong Wang, Xiao-Jun Ji, Chao Ye, Tian-Qiong Shi
Summary: This study successfully engineered an efficient yeast cell factory for overproducing patchoulol using systematic metabolic engineering strategies. By selecting a highly active patchoulol synthase, expanding the mevalonate precursor pool, and optimizing the downregulation of squalene synthesis, the patchoulol titer was significantly increased. The highest reported patchoulol titer of 2.864 g/L was achieved in a 5 L bioreactor.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Biochemical Research Methods
Kaifeng Wang, Tian-Qiong Shi, Lu Lin, Ping Wei, Rodrigo Ledesma-Amaro, Xiao-Jun Ji
Summary: Microbial production of value-added chemicals derived from fatty acids is an alternative to petroleum-derived chemicals and unsustainable animal and plant lipids. Fatty acids with different carbon numbers and chain lengths have wide applications in energy, material, medicine, and nutrition. Metabolic engineering can be used to tailor the chain length of fatty acids, and the oleaginous yeast Yarrowia lipolytica is a suitable host for such production. This Review covers metabolic engineering approaches to control fatty acid chain length in this microorganism.
ACS SYNTHETIC BIOLOGY
(2022)
