Review
Medicine, General & Internal
Robert Ossendorff, Alessandra Menon, Frank A. Schildberg, Pietro S. Randelli, Sebastian Scheidt, Christof Burger, Dieter C. Wirtz, Davide Cucchi
Summary: The global distribution and quality of studies using adipose tissue derivates in orthopedics were assessed through a systematic review. Among the 82 included studies, 70% focused on knee disorders, mainly osteoarthritis. Approximately 26% of the studies investigated expanded adipose-derived stem/stromal cells (ADSCs), with 72% of these using stromal vascular fraction (SVF). The overall quality of the studies varied, with a higher score found in studies involving expanded ADSCs compared to SVF.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Cell Biology
Ming-Li Zou, Si-Yu Liu, Zi-Li Sun, Jun-Jie Wu, Zheng-Dong Yuan, Ying-Ying Teng, Yi Feng, Feng-Lai Yuan
Summary: The incidence of acute and chronic wound diseases is increasing, causing a significant burden on patients, their families, and society due to their complex pathogenesis, long course, and difficult treatment. Research is focusing on identifying new ideas and methods for treatment, with adipose stem cells potentially playing a key role in wound healing.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Cell & Tissue Engineering
Zhaoqi Yuan, Zhu Zhu, Fangxing Zhu, Feixue Ding, Yinmin Wang, Xiuxia Wang, Xusong Luo, Jun Yang, Fei Liu, Di Sun
Summary: The study found that ADSCs can enhance proliferation, migration, invasion, and angiogenesis of DFSP cells, with increased expression of beta-type platelet-derived growth factor receptor, collagen type I alpha 1 chain, vascular endothelial growth factor, hepatocyte growth factor, and basic fibroblast growth factor.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Moema de Alencar Hausen, Ariana de Souza Moraes, Flavia Pedrini, Felipe Grabarz, Jose Angelo Camilli, Eliana Aparecida de Rezende Duek
Summary: This study introduces a natural biomaterial device that can improve the wound healing process by modulating macrophage behavior in vitro. The device, assembled with adipose-derived stem cells and biocompatible polymers, promotes the release of anti-inflammatory cytokines, indicating the prevalence of M2 macrophages.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Polymer Science
Banafsheh Safari, Marziyeh Aghazadeh, Leila Roshangar, Ayuob Aghanejad, Soodabeh Davaran
Summary: This study reports the fabrication and evaluation of a bioactive hybrid scaffold collagen/P-PCL for bone tissue engineering. The scaffold showed biocompatibility, osteoconductivity, and osteoinductivity, supporting the proliferation and differentiation of AD-MSCs.
EUROPEAN POLYMER JOURNAL
(2022)
Review
Biochemistry & Molecular Biology
Katarzyna Czerwiec, Malgorzata Zawrzykraj, Milena Deptula, Aneta Skoniecka, Agata Tyminska, Jacek Zielinski, Adam Kosinski, Michal Pikula
Summary: Adipose-derived mesenchymal stromal cells (AD-MSCs) have gained significant attention due to their easy availability from fat tissue and their regenerative and immunomodulatory properties. These cells show great potential in stem cell-based therapies for wound healing, orthopedic, cardiovascular, and autoimmune diseases. Numerous clinical trials have provided evidence for the effectiveness of AD-MSCs. This article presents current knowledge on AD-MSCs, their applications in pre-clinical models and clinical studies, and highlights the potential for chemically or genetically modified AD-MSCs as the next generation of stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Chenyu Wang, Xiao Long, Loubin Si, Bo Chen, Yiwei Zhang, Tianyu Sun, Xiuqin Zhang, Robert Chunhua Zhao, Xiaojun Wang
Summary: A pilot study comparing the efficacy of fat retention in LoS patients among ADSCs-assisted, SVF-assisted, and conventional AFG methods showed that ADSCs-assisted group had significantly higher fat retention rates. No severe adverse events were reported, indicating the safety and feasibility of ADSCs-assisted AFG for facial atrophy correction in LoS patients.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Cell & Tissue Engineering
Jana C. Blum, Thilo L. Schenck, Alexandra Birt, Riccardo E. Giunta, Paul S. Wiggenhauser
Summary: In this study, the combination of cell-derived artificial extracellular matrix with 3D printed polycaprolactone scaffolds showed promising results in improving bioactivity and differentiation of human adipose derived stem cells. The ac/aoPCL scaffolds demonstrated good biocompatibility and enhanced chondrogenic and osteogenic differentiation potential.
JOURNAL OF TISSUE ENGINEERING
(2021)
Article
Chemistry, Physical
Jing Ren, Jinghan Chi, Baishi Wang, Lingli Guo, Yudi Han, Xinhui Liu, Xuetao Pei, Wen Yue, Yan Han
Summary: This study demonstrated that the combination of human adipose-derived stem cells (hASCs) and a human decellularized adipose tissue matrix (hDAM) could accelerate wound healing and promote angiogenesis, showing potential for clinical application.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Cell Biology
Afzaal Bashir, Muhammad M. M. Bashir, Sunaina Afzaal, Fridoon J. J. Ahmad, Mahmood S. S. Choudhery
Summary: This study compared the long-term effects of conventional adipose tissue grafting and ex-vivo expanded ASC-enriched adipose tissue grafting for the treatment of contour deformities related pigmentary changes on the face. The results showed that autologous fat grafting enriched with adipose stem cells is the preferred option for improving contour deformities associated with increased pigmentation of the face skin.
CELL BIOLOGY INTERNATIONAL
(2023)
Article
Cell Biology
Anna Labedz-Maslowska, Agnieszka Szkaradek, Tomasz Mierzwinski, Zbigniew Madeja, Ewa Zuba-Surma
Summary: This study optimized the protocols for processing and ex vivo expansion of adipose tissue-derived mesenchymal stem cells (AT-MSCs) for the development of an Advanced Therapy Medicinal Product (ATMP) for human use. Critical process parameters were identified and examined to ensure the quality of the final product containing AT-MSCs.
Article
Medicine, Research & Experimental
Francesca Toia, Elena Lo Presti, Anna Barbara Di Stefano, Marta Di Simone, Marco Trapani, Anna Maria Corsale, Carmela Picone, Francesco Moschella, Francesco Dieli, Adriana Cordova, Serena Meraviglia
Summary: This study analysed the immunomodulatory properties of spheroids from adipose stem cells (SASCs) and compared them to adipose stem cells (ADSCs). SASCs showed stronger immunomodulatory abilities in heterologous experimental settings compared to ADSCs. SASCs also demonstrated greater anti-inflammatory properties.
Article
Cell & Tissue Engineering
Naoki Ishiuchi, Ayumu Nakashima, Satoshi Maeda, Yoshie Miura, Kisho Miyasako, Kensuke Sasaki, Toshio Uchiki, Ayano Sasaki, Shogo Nagamatsu, Naoki Nakao, Masataka Nagao, Takao Masaki
Summary: Fibrosis is a common histological feature in chronic organ injury, leading to organ failure. Adipose-derived mesenchymal stem cells (ASCs) have been studied as a therapeutic tool to prevent organ failure. This study compared the therapeutic effects of ASCs from superficial and deep layers and found that both types of ASCs are equally effective and safe for treating organ injury.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biotechnology & Applied Microbiology
Qiulin He, Junxin Lin, Fanghao Zhou, Dandan Cai, Yiyang Yan, Yejie Shan, Shufang Zhang, Tiefeng Li, Xudong Yao, Hongwei Ouyang
Summary: Nonuniform microstretching (NUMS), a natural phenomenon in bone tissues, promotes osteogenic differentiation and has potential positive effects on bone healing.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Seita Osawa, Hisashi Kato, Yuki Maeda, Hisashi Takakura, Junetsu Ogasawara, Tetsuya Izawa
Summary: This study investigated the impact of exercise training and high-fat diet on the differentiation potential of adipose-derived stem cells (ADSCs) and found variations in amino acid accumulation under different conditions. The findings also revealed the influence of exercise training on amino acid metabolism pathways in ADSCs-derived adipocytes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Atsushi Fuku, Yasuhiko Taki, Yuka Nakamura, Hironori Kitajima, Takashi Takaki, Terutsugu Koya, Ikuhiro Tanida, Kaori Nozaki, Hiroshi Sunami, Hiroaki Hirata, Yoshiyuki Tachi, Togen Masauji, Naoki Yamamoto, Yasuhito Ishigaki, Shigetaka Shimodaira, Yusuke Shimizu, Toru Ichiseki, Ayumi Kaneuji, Satoshi Osawa, Norio Kawahara
Summary: This study utilized a novel method to form human ADSC spheroids and identified their characteristics and effects through gene expression analysis and cytokine analysis. The study found that ADSC spheroids could be a promising treatment option for osteoarthritis, but further research on administration methods and their interaction with synovial fluid is necessary.
Article
Oncology
Shimpei Gotoh, Toshiyuki Nakasone, Akira Matayoshi, Shoko Makishi, Fusahiro Hirano, Edward Ntege, Yusuke Shimizu, Hiroyuki Nakamura
Summary: Mucoepidermoid carcinomas (MECs) are rare malignant tumors that were originally considered as benign. Diagnosis of MECs is challenging, especially for intraoral MECs. This case report highlights the importance of accurate histopathological evaluation for the diagnosis of MECs.
MOLECULAR AND CLINICAL ONCOLOGY
(2022)
Review
Oncology
Shimpei Gotoh, Edward Ntege, Toshiyuki Nakasone, Akira Matayoshi, Sho Miyamoto, Yusuke Shimizu, Hiroyuki Nakamura
Summary: This report describes a rare case of chondroid syringoma (CS) of the lower lip, highlighting the importance of proper diagnosis, treatment, and prognostic evaluation. Surgical excision biopsy confirmed the diagnosis of benign CS, and no recurrence was observed during the 2-year follow-up period.
MOLECULAR AND CLINICAL ONCOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Makoto Murahashi, Edward Hosea Ntege, Masaru Higa, Nobuyuki Maruyama, Toshihiro Kawano, Yusuke Shimizu, Hiroyuki Nakamura
Summary: The coexistence of calcium pyrophosphate dihydrate crystal deposition (CPP) and synovial chondromatosis (SC) in the temporomandibular joint (TMJ) is rare. This report presents a case of a 61-year-old man with coexisting CPP and SC in the TMJ. The patient experienced pain and trismus in the right TMJ and was initially diagnosed with SC based on radiographic assessments. However, histopathology revealed deposits of rhomboid and rod-shaped crystals positive for birefringence, confirming the coexistence of CPP. Surgical removal of loose bodies and chalk-like lesions was performed and after a follow-up of 1.5 years, no recurrence was observed.
Article
Cell Biology
Hironori Kitajima, Takuya Sakamoto, Tetsuhiro Horie, Ayane Kuwano, Atsushi Fuku, Yasuhiko Taki, Yuka Nakamura, Ikuhiro Tanida, Hiroshi Sunami, Hiroaki Hirata, Yoshiyuki Tachi, Naoki Yamamoto, Yasuo Iida, Yasuhito Ishigaki, Sohsuke Yamada, Shigetaka Shimodaira, Yusuke Shimizu, Toru Ichiseki, Ayumi Kaneuji, Satoshi Osawa, Norio Kawahara
Summary: Knee osteoarthritis (Knee OA) is a condition that causes bone deformity and degradation of articular cartilage, resulting in chronic pain and movement disorders. Administering cultured adipose-derived stem cells (ADSCs) into the knee joint cavity can improve the symptoms of Knee OA, but the effect of synovial fluid (SF) on the injected ADSCs remains unclear.
Review
Cell & Tissue Engineering
Yusuke Shimizu, Edward Hosea Ntege, Hiroshi Sunami, Yoshikazu Inoue
Summary: Hair loss, associated with psychosocial and medical comorbidities, is a burdensome condition economically. Conventional medical interventions for alopecia have limitations, thus regenerative treatments like mesenchymal stem cell implantation, MSC-derived secretome treatment, and platelet-rich plasma therapies are being explored. This review summarizes the most promising regenerative treatments for alopecia.
REGENERATIVE THERAPY
(2022)
Review
Cell & Tissue Engineering
Yusuke Shimizu, Edward Hosea Ntege, Hiroshi Sunami
Summary: There is a growing interest among healthcare specialists and the public in using regenerative medicine-based approaches for skin regeneration. These approaches can be categorized into cell-based and cell-free therapies, and are reported to be safe and effective. This review provides an update on the different regenerative medicine-based therapies for skin regeneration and highlights the regulatory perspective on the clinical application of some of these therapies in Japan.
REGENERATIVE THERAPY
(2022)
Article
Oncology
Sho Miyamoto, Yoshihiko Hirohashi, Rena Morita, Akihiro Miyazaki, Kazuhiro Ogi, Takayuki Kanaseki, Kentaro Ide, Jumpei Shirakawa, Tomohide Tsukahara, Aiko Murai, Takashi Sasaya, Kazushige Koike, Shinichiro Kina, Toshihiro Kawano, Takahiro Goto, Edward Hosea Ntege, Yusuke Shimizu, Toshihiko Torigoe
Summary: Despite advances in cancer therapies, the mortality rate of oral cancer has not improved in the past three decades. Oral cancers contain a subpopulation of cancer stem cells (CSCs) that contribute to cancer infiltration, recurrence, metastasis, and drug resistance. Cytotoxic CD8+ T lymphocytes (CTLs) play a crucial role in targeting CSCs and regulating cancer progression. This study investigates the potential of olfactory receptor family 7 subfamily C member 1 (OR7C1) as a target for CSC-targeted immunotherapy in oral cancer and finds that OR7C1 is expressed only in oral CSCs and is effectively recognized by CTLs.
Article
Oncology
Kentaro Ide, Toshihiro Kawano, Jumpei Shirakawa, Edward Hosea Ntege, Sho Miyamoto, Taro Ikegami, Hiroshi Sunami, Mikio Suzuki, Yusuke Shimizu, Hiroyuki Nakamura
Summary: Despite advances in therapy, oral neoplasms remain a serious threat to individuals worldwide. This study investigates the role of SSEA3 in the progression and management of oral malignancies, potentially opening doors for more effective treatment.
Article
Medicine, General & Internal
Rikako Matsuura, Yusuke Shimizu, Naoki Matsuura, Edward Hosea Ntege, Naoki Wada
Summary: This report presents a case of a 10-year-old girl with Poland syndrome manifesting an axillary web containing a fibrous band, which was successfully corrected by a double-opposing Z-plasty. Histopathological findings indicated the presence of tendon-like tissue, highlighting the importance of appropriate treatment approaches for Poland syndrome. Addressing both tendon and skin components is essential for optimal contracture release.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Cell Biology
Yusuke Shimizu, Edward Hosea Ntege, Chinatsu Azuma, Fuminari Uehara, Takashi Toma, Kotaro Higa, Hiroki Yabiku, Naoki Matsuura, Yoshikazu Inoue, Hiroshi Sunami
Summary: Rheumatoid arthritis is a chronic autoimmune disorder that primarily affects joints and muscles. Conventional treatments only address symptoms, and some patients experience adverse effects or poor responses. Transplanting multipotent adult stem cells, such as mesenchymal stromal/stem cells, has shown promise in controlling inflammation and promoting tissue regeneration in patients with refractory diseases, including rheumatoid arthritis. This review discusses the current status and challenges of MSC-based therapies for rheumatoid arthritis, offering opportunities for broader clinical application.
Article
Cell & Tissue Engineering
Jinghui Xu, Zhenxiao Ren, Tianzuo Niu, Siyuan Li
Summary: This study discovers that MSCs-EVs attenuate inflammation and oxidative stress in spinal cord injury (SCI) by delivering miR-26b-5p to target the KDM6A/NOX4 axis, facilitating recovery.
REGENERATIVE THERAPY
(2024)
Article
Cell & Tissue Engineering
Shant Nepal, Jinyan Si, Shohei Ishikawa, Masaki Nishikawa, Yasuyuki Sakai, Aya M. Akimoto, Hiroyuki Okada, Shinsuke Ohba, Ung-il Chung, Takamasa Sakai, Hironori Hojo
Summary: In this study, we evaluated the potential of oligo-tetra-PEG gel (Oligo gel) as a growth factor-releasing scaffold. Compared to tetra-PEG hydrogel (Tetra gel), Oligo gel underwent a higher degree of gel-gel phase separation, demonstrating better protein release and osteogenic potential, and achieving superior regeneration in a mouse calvarial defect model. Therefore, Oligo gel is considered a promising therapeutic biomaterial.
REGENERATIVE THERAPY
(2024)
Article
Cell & Tissue Engineering
Yasuhiro Homma, Sayuri Uchino, Masashi Nagao, Takanori Wakayama, Shin Fukusato, Tomonori Baba, Taiji Watari, Koju Hayashi, Yoshitomo Saita, Muneaki Ishijima
Summary: This study investigates the safety and feasibility of locoregional PRP injection for iliopsoas impingement after THA, and finds that it is both safe and feasible.
REGENERATIVE THERAPY
(2024)
Article
Cell & Tissue Engineering
Taichi Tenkumo, Benedikt Kruse, Kathrin Kostka, Viktoriya Sokolova, Toru Ogawa, Nobuhiro Yoda, Oleg Prymak, Osamu Suzuki, Keiichi Sasaki, Matthias Epple
Summary: This study evaluated the bone-healing effects of a triple-functionalized CaP paste in the rat femoral head. The results showed that the paste significantly accelerated bone healing after 21 days and increased the levels of bone formation-related markers at the protein and gene levels.
REGENERATIVE THERAPY
(2024)