Review
Cell Biology
Haifeng Zeng, Shaoshen Guo, Xuancheng Ren, Zhenkun Wu, Shuwen Liu, Xingang Yao
Summary: Extracellular vesicles, including ectosomes and exosomes, have emerged as promising natural carriers for drug delivery. Exosomes, which are 30-100 nm in diameter and possess a lipid bilayer, have high biocompatibility, stability, and low immunogenicity, making them desirable for cargo delivery. Loading cargo into exosomes, however, remains challenging despite various strategies developed. This review provides an overview of current cargo delivery strategies using exosomes and summarizes recent approaches for loading small-molecule, nucleic acid, and protein drugs into exosomes, offering insights for more efficient drug delivery.
Article
Cell Biology
Chaoxiang Chen, Mengdi Sun, Jialin Wang, Liyun Su, Junjie Lin, Xiaomei Yan
Summary: Extracellular vesicles have unique advantages as nanocarriers for drug delivery, but their cargo encapsulation efficiency is often below expectations, especially for hydrophilic chemotherapeutic drugs. This study introduced a new method named SEAL, which significantly enhances drug encapsulation efficiency of EVs, and single-particle analysis techniques were used to uncover the encapsulation heterogeneity of EVs.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Review
Neurosciences
Jereme G. Spiers, Natasha Vassileff, Andrew F. Hill
Summary: Neuroinflammation involves coordinated responses from multiple cell types and cellular communication mechanisms. Extracellular vesicles (EVs) have emerged as important regulators of the inflammatory response, delivering selectively packaged proteins, miRNA, and lipids between different cells and tissues.
NEUROMOLECULAR MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Yeongchang Goh, Jongwoo Kim, Hye Sun Park, Taeyoung Jung, Kwan Soo Hong, Sang Hwan Nam, Yung Doug Suh, Kang Taek Lee
Summary: Cell-cell communication is crucial for cellular differentiation, organ function, and immune responses. This study demonstrates the use of dual-colour imaging with upconverting nanoparticles to visualize the intercellular transport of cargo molecules, and shows that extracellular vesicles can be transmitted along with fluorescently labelled proteins.
Article
Medicine, Research & Experimental
Masaharu Somiya, Shun'ichi Kuroda
Summary: In this study, the FRB-FKBP system was utilized to sort bioactive proteins into EVs, enabling successful protein delivery with confirmed functionality.
MOLECULAR PHARMACEUTICS
(2022)
Review
Biochemistry & Molecular Biology
Sarah A. Walsh, Benjamin W. Hoyt, Cassie J. Rowe, Devaveena Dey, Thomas A. Davis
Summary: Severe polytraumatic injury can lead to immune dysfunction, but early biomarkers for immune dysfunction are currently lacking. Study of exosomal cargo may provide critical insights for intervention.
Article
Cell Biology
Andreia M. Silva, Elisa Lazaro-Ibanez, Anders Gunnarsson, Aditya Dhande, George Daaboul, Ben Peacock, Xabier Osteikoetxea, Nikki Salmond, Kristina Pagh Friis, Olga Shatnyeva, Niek Dekker
Summary: This study introduced a workflow for accurately quantifying the efficiency of different EV-sorting proteins in promoting cargo loading into EVs. By using a combination of techniques, TSPAN14, CD63, and CD63/CD81 fused to the PDGFR beta transmembrane domain were identified as the most efficient EV-sorting proteins, accumulating an average of 50-170 single GFP molecules per vesicle.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Cell Biology
Jifeng Xu, Yu Chen, Dongsheng Yu, Li Zhang, Xiaofan Dou, Gang Wu, Yaping Wang, Shuijun Zhang
Summary: The study revealed that osteoporotic patients had significantly higher concentrations of EVs in plasma, which showed a linear correlation with bone mineral density values. The expression levels of miRNAs and Wnt signals were associated with osteoporosis, indicating that osteoporosis increases the secretion of EVs that carry specific molecules affecting mineralization capacity.
Article
Cell & Tissue Engineering
Lihao Chen, Weihan Xie, Keke Wu, Yuan Meng, Yijun He, Jiawei Cai, Yuan Jiang, Qi Zhao, Yixi Yang, Minru Zhang, Manping Lu, Shaozhang Lin, Lin Liang, Zhiyong Zhang
Summary: Extracellular vesicle (EV) therapy has recently shown effectiveness in various diseases. However, the commonly used method of collecting EVs, serum starvation culture (SC), may have inadvertent effects and reduce the therapeutic potential of EVs. Therefore, a novel method called continuous nutrient supply culture (CC) was developed to provide optimal conditions for mesenchymal stem cells (MSCs) by continuously supplying essential nutrients.
JOURNAL OF TISSUE ENGINEERING
(2023)
Review
Endocrinology & Metabolism
Rebecca S. Aguirre, Abhishek Kulkarni, Matthew W. Becker, Xiaoyong Lei, Soumyadeep Sarkar, Sasanka Ramanadham, Edward A. Phelps, Ernesto S. Nakayasu, Emily K. Sims, Raghavendra G. Mirmira
Summary: Islet β cells play a crucial role in the pathogenesis of type 1 diabetes, with extracellular vesicles (EVs) potentially serving as an important mechanism for cellular communication. Understanding the formation and cargo of EVs may offer new insights into T1D pathogenesis and potentially lead to novel therapeutic approaches. Future research in EV biology could provide opportunities for intervention in T1D.
MOLECULAR METABOLISM
(2022)
Article
Engineering, Biomedical
Christopher Millan, Lukas Prause, Queralt Vallmajo-Martin, Natalie Hensky, Daniel Eberli
Summary: Engineered microtissues have a significant impact on extracellular vesicles (EVs) secreted by benign and malignant prostate cells, with a higher yield of EVs per cell in 3D cultures compared to 2D. Whole transcriptome sequencing and proteomics analysis reveal stark contrasts in molecular cargo between 2D-EVs and 3D-EVs, with exclusive enrichment of known drivers of prostate cancer progression in 3D-EVs. Functional assays demonstrate that only 3D-EVs confer increased invasiveness and chemoresistance to cells in 2D, highlighting the potential of engineered 3D microtissue cultures for identifying biomarkers not detectable in standard 2D conditions.
ADVANCED HEALTHCARE MATERIALS
(2022)
Review
Endocrinology & Metabolism
Rebecca S. Aguirre, Abhishek Kulkarni, Matthew W. Becker, Xiaoyong Lei, Soumyadeep Sarkar, Sasanka Ramanadham, Edward A. Phelps, Ernesto S. Nakayasu, Emily K. Sims, Raghavendra G. Mirmira
Summary: This review examines the impact of extracellular vesicles (EVs) released by pancreatic islet beta cells on the development of type 1 diabetes (T1D). The formation and cargo of EVs play important roles in the pathogenesis of T1D and have the potential to serve as biomarkers or therapeutic candidates.
MOLECULAR METABOLISM
(2022)
Review
Biochemistry & Molecular Biology
Sara Monteiro-Reis, Carina Carvalho-Maia, Genevieve Bart, Seppo J. Vainio, Juliana Pedro, Eunice R. Silva, Goreti Sales, Rui Henrique, Carmen Jeronimo
Summary: EVs, as heterogeneous cell-derived membranous granules, play a crucial role in CNS-related disorders and have been studied for their molecular components in disease progression and therapeutic applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Masaharu Somiya, Shun'ichi Kuroda
Summary: This study developed an EV cargo delivery assay using a NanoBiT system, enabling real-time quantitative measurement of EV cargo delivery efficiency. It was found that modification of EVs with a virus-derived fusogenic protein significantly enhanced cytoplasmic cargo delivery.
ANALYTICAL CHEMISTRY
(2021)
Review
Oncology
Maria Harmati, Matyas Bukva, Timea Boroczky, Krisztina Buzas, Edina Gyukity-Sebestyen
Summary: Metabolomic reprogramming in tumor and stroma cells is a key aspect of cancer development, but the study of metabolite composition in tumor-derived extracellular vesicles (EVs) is still nascent. While EVs play a crucial role in intercellular communication, particularly in tumor progression, there is limited understanding of the biological activity of tumor EV metabolites.
CANCER AND METASTASIS REVIEWS
(2021)
Editorial Material
Cardiac & Cardiovascular Systems
Heidi Noels, Peter Boor, Claudia Goettsch, Mathias Hohl, Wilhelm Jahnen-Dechent, Vera Jankowski, Ingrid Kindermann, Rafael Kramann, Michael Lehrke, Dominik Linz, Christoph Maack, Barbara Niemeyer, Leticia P. Roma, Katharina Schuett, Thimoteus Speer, Stefan Wagenpfeil, Christian Werner, Stephen Zewinger, Michael Boehm, Nikolaus Marx, Juergen Floege, Danilo Fliser, Joachim Jankowski
EUROPEAN HEART JOURNAL
(2018)
Review
Hematology
Claudia Goettsch, Mads Kjolby, Elena Aikawa
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2018)
Review
Multidisciplinary Sciences
Sebastian Wendt, Andreas Goetzenich, Claudia Goettsch, Christian Stoppe, Christian Bleilevens, Sandra Kraemer, Carina Benstoem
SCIENTIFIC REPORTS
(2018)
Article
Food Science & Technology
Marieke Sternkopf, Sven Thoroee-Boveleth, Tobias Beck, Kirsten Oleschko, Ansgar Erlenkoetter, Ulrich Tschulena, Sonja Steppan, Thimoteus Speer, Claudia Goettsch, Vera Jankowski, Joachim Jankowski, Heidi Noels
Review
Food Science & Technology
Anika Himmelsbach, Carina Ciliox, Claudia Goettsch
Article
Biochemistry & Molecular Biology
Mathias Hohl, Manuel Mayr, Lisa Lang, Alexander G. Nickel, Javier Barallobre-Barreiro, Xiaoke Yin, Thimoteus Speer, Simina-Ramona Selejan, Claudia Goettsch, Katharina Erb, Claudia Fecher-Trost, Jan-Christian Reil, Benedikt Linz, Sven Ruf, Thomas Huebschle, Christoph Maack, Michael Boehm, Thorsten Sadowski, Dominik Linz
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Endocrinology & Metabolism
Ben Arpad Kappel, Lorenzo De Angelis, Michael Heiser, Marta Ballanti, Robert Stoehr, Claudia Goettsch, Maria Mavilio, Anna Artati, Omero A. Paoluzi, Jerzy Adamski, Geltrude Mingrone, Bart Staels, Remy Burcelin, Giovanni Monteleone, Rossella Menghini, Nikolaus Marx, Massimo Federici
MOLECULAR METABOLISM
(2020)
Article
Cell Biology
Marina A. Heuschkel, Nikolaos T. Skenteris, Joshua D. Hutcheson, Dewy D. van der Valk, Juliane Bremer, Philip Goody, Jesper Hjortnaes, Felix Jansen, Carlijn V. C. Bouten, Antoon van den Bogaerdt, Ljubica Matic, Nikolaus Marx, Claudia Goettsch
Article
Cardiac & Cardiovascular Systems
Maximillian A. Rogers, Joshua D. Hutcheson, Takehito Okui, Claudia Goettsch, Sasha A. Singh, Arda Halu, Florian Schlotter, Hideyuki Higashi, Lixiang Wang, Mary C. Whelan, Andrew K. Mlynarchik, Alan Daugherty, Masatoshi Nomura, Masanori Aikawa, Elena Aikawa
Summary: The study demonstrates that inhibition of the mitochondrial fission protein DRP1 can reduce remodeling of specific ER microdomains, promoting mitochondrial fission, autophagy, and enhancing mitochondrial calcium buffering and PCSK9 degradation. The findings suggest a novel role of DRP1 in regulating hepatic proteostasis and mdivi-1 as a potential small molecule PCSK9 inhibitor.
CARDIOVASCULAR RESEARCH
(2021)
Article
Cardiac & Cardiovascular Systems
Farwah Iqbal, Florian Schlotter, Dakota Becker-Greene, Adrien Lupieri, Claudia Goettsch, Joshua D. Hutcheson, Maximillian A. Rogers, Shinsuke Itoh, Arda Halu, Lang Ho Lee, Mark C. Blaser, Andrew K. Mlynarchik, Sumihiko Hagita, Shiori Kuraoka, Hao Yu Chen, James C. Engert, Livia S. A. Passos, Prabhash K. Jha, Eric A. Osborn, Farouc A. Jaffer, Simon C. Body, Simon C. Robson, George Thanassoulis, Masanori Aikawa, Sasha A. Singh, Abhijeet R. Sonawane, Elena Aikawa
Summary: This study identified SORT1 as a key player in the pathophysiology of calcific aortic valve disease (CAVD) and explored its role in the transformation of valvular interstitial cells (VICs) into pathological phenotypes. It was found that SORT1 promotes CAVD through mediating valvular fibrosis and calcification. This study provides insight into the role of SORT1 in valve calcification and suggests that it could be a potential therapeutic target to inhibit the emergence of pathological phenotypes underlying CAVD.
EUROPEAN HEART JOURNAL
(2023)
Review
Biochemistry & Molecular Biology
Jonas Heyn, Marina Augusto Heuschkel, Claudia Goettsch
Summary: Mitochondria play crucial roles in regulating metabolism, cell death, and energy production. The maintenance of mitochondrial health is essential for cellular homeostasis. Recent studies have identified a novel cellular response called mitochondrial-derived vesicles (MDVs), which are released from mitochondria and can be degraded within lysosomes or peroxisomes. MDVs are implicated in cardiovascular disease (CVD) and may contribute to mitochondrial dysfunction. Furthermore, extracellular vesicles (EVs) have been found to contain mitochondrial content. This article provides an overview of MDV formation and trafficking, discusses the connection between MDVs and EV biogenesis, and explores their roles in CVD, as well as the potential cardioprotective effects of vesicle-mediated mitochondrial transfer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cardiac & Cardiovascular Systems
Joshua D. D. Hutcheson, Claudia Goettsch
Summary: Patients with CKD have a significantly increased risk of cardiovascular disease, primarily due to premature aging of blood vessels and heart and accelerated ectopic calcification. The presence of cardiovascular calcification is associated with higher risk in CKD patients. Disturbances in mineral homeostasis and comorbidities contribute to systemic cardiovascular calcification with various clinical consequences. This review discusses the heterogeneity in calcification patterns, the impact of mineral on tissue function, and potential therapeutic approaches to disrupt mineral nucleation and growth in CKD patients.
CIRCULATION RESEARCH
(2023)
Article
Hematology
Julian Kamhieh-Milz, Lei Chen, Claudia Goettsch, Anna Maria Pfefferkorn, Anja Hofmann, Coy Brunssen, Gregor M. Mueller, Thomas Walther, Muhammad Imtiaz Ashraf, Guido Moll, Henning Morawietz, Janusz Witowski, Rusan Catar
Summary: This study analyzed the mechanism by which NOX2 contributes to Ang II-induced ET-1 production in human microvascular endothelial cells. The results showed that high-fat diet increased the expression of Ang II and ET-1 in wild-type mice, but not in Nox2-deficient mice. In vitro experiments revealed that Ang II promoted NOX2 expression through induction of the Oct-1 protein and increased production of superoxide anions. Inhibition of Oct-1 and superoxide attenuated Ang II-induced ET-1 production.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Respiratory System
Alexandra Stroda, Vincent Brandenburg, Ayham Daher, Christian Cornelissen, Claudia Goettsch, Andras Keszei, Michael Dreher
RESPIRATORY RESEARCH
(2018)
