Article
Medicine, Research & Experimental
Tingting Xu, Mengyan Xie, Xinming Jing, Huning Jiang, Xi Wu, Xinzhu Wang, Yongqian Shu
Summary: In this study, bioinformatic analysis and experimental validation revealed that miR-26b-5p was downregulated in Helicobacter pylori-infected gastric cancer (GC) cells and GC tissues. Low expression of miR-26b-5p promoted GC proliferation in vitro and in vivo and was associated with poor prognosis in GC patients. Mechanistically, miR-26b-5p directly targeted PDE4B and CDK8, leading to decreased phosphorylation and nuclear translocation of STAT3, which regulated GC proliferation. Importantly, miR-26b-5p was transcriptionally suppressed by STAT3, forming a miR-26b-5p-PDE4B/CDK8-STAT3 positive feedback loop. This newly identified feedback loop may serve as a promising therapeutic target for inflammation-related GC progression.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Cell Biology
Sukanya Shyamasundar, Seshadri Ramya, Deepika Kandilya, Dinesh Kumar Srinivasan, Boon Huat Bay, Suraiya Anjum Ansari, S. Thameem Dheen
Summary: Maternal diabetes is associated with increased risk of neurodevelopmental disorders in offspring. Hyperglycemia alters expression of genes and microRNAs in neural stem cells, including downregulation of methyl-CpG-binding protein-2 (Mecp2). miR-26b-5p controls Mecp2 expression and affects neurite outgrowth and synaptogenesis. These findings suggest that maternal diabetes disrupts synaptogenesis through miR-26b-5p/Mecp2 pathway, potentially leading to neurodevelopmental disorders.
Article
Clinical Neurology
Rukiye Tekdemir, Yavuz Selvi, Kursat Altinbas, Nadir Kocak
Summary: This study found that miRNAs play an important role in the pathogenesis of bipolar disorder and may serve as biomarkers for diagnosis and screening of the disorder.
JOURNAL OF AFFECTIVE DISORDERS
(2022)
Article
Immunology
Rachel E. Crossland, Jean Norden, Sakhila Ghimire, Mateja Kralj Juric, Kim F. Pearce, Clare Lendrem, Matthew Collin, Eva Mischak-Weissinger, Ernst Holler, Hildegard T. Greinix, Anne M. Dickinson
Summary: The study found that miR-155 and miR-146a were upregulated in gastrointestinal and skin tissue samples of aGvHD patients, and their expression levels in serum and urine samples were also associated with the risk of developing aGvHD. These results support the potential of miR-155 and miR-146a as biomarkers for aGvHD, but further investigation is needed to understand their role in generalized inflammation and specific pathophysiology.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Daniel G. Henriques, Renan Lyra Miranda, Romulo Sperduto Dezonne, Luiz Eduardo Wildemberg, Aline Helen da Silva Camacho, Leila Chimelli, Leandro Kasuki, Elisa B. Lamback, Alexandro Guterres, Monica R. Gadelha
Summary: This study identified miRNAs associated with resistance to fg-SRLs treatment in acromegaly using miRNA sequencing and bioinformatic analysis. It was found that miR-181a-5p, miR-181b-5p, and miR-383-5p were differentially expressed, with miR-181a-5p and miR-181b-5p downregulated and miR-383-5p upregulated in the uncontrolled group. ROC curve analysis showed that miR-383-5p had an NPV of 84.3% and a PPV of 84.5%. Thus, these miRNAs can serve as biomarkers and tools to guide clinical decisions in response to fg-SRLs treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Tianxiang Chen, Runkun Liu, Yongshen Niu, Huanye Mo, Hao Wang, Ye Lu, Liang Wang, Liankang Sun, Yufeng Wang, Kangsheng Tu, Qingguang Liu
Summary: This study identified a novel lncRNA, KDM4A-AS1, which is highly expressed in HCC tissues and plays a crucial role in promoting tumor growth and metastasis by competitively binding miR-411-5p, thereby activating the AKT pathway. Additionally, KDM4A-AS1 was found to be regulated by hypoxia-inducible factor 1 alpha (HIF-1 alpha) through the KPNA2/AKT signaling pathway, providing a potential prognostic and therapeutic target for HCC.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Yu-Jen Chen, Chia-Tien Hsu, Shang-Feng Tsai, Cheng-Hsu Chen
Summary: Chronic allograft dysfunction (CAD) is a major condition affecting long-term kidney graft survival. In this study, the correlation between plasma miR-21 and the severity of CAD was investigated. RNA sequencing was performed and differentially expressed miRNAs in CAD were identified. MiR-21-5p, miR-20a-5p, and miR-101-3p were found to be associated with the TGF-beta pathway and CAD. These findings may lead to the development of early prediction miRNA biomarkers and therapeutic tools for CAD in the field of kidney transplantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Camila Meirelles S. Silva, Mateus C. Barros-Filho, Deysi Viviana T. Wong, Julia Bette H. Mello, Livia Maria S. Nobre, Carlos Wagner S. Wanderley, Larisse T. Lucetti, Heitor A. Muniz, Igor Kenned D. Paiva, Hellen Kuasne, Daniel Paula P. Ferreira, Maria Perpetuo S. S. Cunha, Carlos G. Hirth, Paulo Goberlanio B. Silva, Rosane O. Sant'Ana, Marcellus Henrique L. P. Souza, Josiane S. Quetz, Silvia R. Rogatto, Roberto Cesar P. Lima-Junior
Summary: By screening miRNAs in plasma, we identified four miRNAs as potential biomarkers for the early detection of colorectal cancer, presenting high performance and easy applicability in clinical practice.
Article
Biotechnology & Applied Microbiology
Gaofeng Tang, Huibo Zhao, Zhantao Xie, Sidong Wei, Guoyong Chen
Summary: HAGLROS contributes to malignant progression of hepatocellular carcinoma (HCC) by serving as a sponge for miR-26b-5p to facilitate KPNA2 expression and inactivate p53 signaling.
Article
Plant Sciences
Xiaojing Jia, Wei Shao, Suqing Tian
Summary: Berberine has protective effects on heart ischemia-reperfusion injury by inducing miR-26b-5p and suppressing the PTGS2/MAPK pathway. These findings support the application of berberine as an anti-I/R agent.
PHARMACEUTICAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Ayman Mohamed Metwally, Ameera Abdel Hamed Mahmoud Kasem, Magda Ismail Youssif, Safia Mohammed Hassan, Abdel Hady A. Abdel Wahab, Lobna Ahmed Refaat
Summary: The study aimed to determine the prognostic significance of serum miR-222-3p, miR-26b-5p, EBV-miR-BHRF1-2-5p, and EBV-miR-BHRF1-2-3p in DLBCL and correlate their levels with clinical and haematological markers. The study also investigated the role of BM BMI1 and PIM2 proteins in predicting BM infiltration. It was found that all the studied miRNAs were dysregulated in DLBCL serum samples.
SCIENTIFIC REPORTS
(2023)
Article
Immunology
Zhongqiang Zhang, Bo Yang, Shuping Zhou, Junxing Wu
Summary: The study revealed that circular RNA_SEC24A plays a promoting role in the progression of osteoarthritis, through the circ_SEC24A/miR-26b-5p/DNMT3A ceRNA axis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Virology
Aunji Pradhan, Ashish Aneja, Sahana Ghosh, Himadri Devvanshi, C. Deepika, Risabh Sahu, Celil Ross, Pallavi Kshetrapal, Arindam Maitra, Saumitra Das
Summary: In this study, the role of exosomal miRNAs in dengue virus-induced disease progression was analyzed in an Indian cohort. After comprehensive analysis, miR-96-5p was found to be upregulated while miR-146a-5p was downregulated with the progression of disease to severe form. The expression levels of miR-96-5p and miR-146a-5p were strongly correlated with platelet levels. Additionally, miR-146a-5p was found to regulate the expression of proteins involved in immune responses.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yi Liu, Gongming Zhang, Hao Chen, Hua Wang
Summary: Our study revealed that DUXAP8 promotes tumor progression in LUAD by targeting miR-26b-5p. This provides a novel therapeutic target for the diagnosis and therapy of LUAD.
BIOSCIENCE REPORTS
(2021)
Article
Neurosciences
Zhirong Wan, Madiha Rasheed, Yumeng Li, Qin Li, Peifu Wang, Jilai Li, Zixuan Chen, Jichen Du, Yulin Deng
Summary: Depression is a common but often neglected symptom of Parkinson's disease (PD), and there is a need for suitable diagnostic biomarkers. This study aimed to assess the potential of brain-enriched miR-218-5p and miR-320-5p as biomarkers for depression in Chinese PD patients. The results showed downregulation of miR-218-5p and miR-320-5p and upregulation of IL-6 and S100B in depressed PD patients. These miRNAs were negatively correlated with depression scores and IL-6 levels, and positively correlated with PD duration and medication. ROC analysis demonstrated their potential as diagnostic biomarkers. Furthermore, in silico analysis identified key neurological pathways regulated by the targets of these miRNAs. In conclusion, miR-218-5p and miR-320-5p may serve as future biomarkers for depression in PD, aiding in early diagnosis and treatment.
MOLECULAR NEUROBIOLOGY
(2023)
Letter
Hematology
Gerben Duns, Melanie Winkle, Lauren Chong, Daisuke Ennishi, Ryan D. D. Morin, Arjan Diepstra, David W. W. Scott, Joost L. L. Kluiver, Christian Steidl, Anke van den Berg
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Bibliography
Oncology
Malgorzata Dawidowska, Agnieszka Dzikiewicz-Krawczyk, Natalia Rozwadowska, Panagiotis Ntziachristos, Joost Kluiver, Anke van den Berg, Reiner Siebert, Maciej Giefing
Article
Biochemistry & Molecular Biology
Tomasz Wozniak, Weronika Sura, Marta Kazimierska, Marta Elzbieta Kasprzyk, Marta Podralska, Agnieszka Dzikiewicz-Krawczyk
Summary: In this study, we developed an online tool called transCRISPR for searching sequence motifs and designing sgRNAs in user-provided genomic regions. The tool provides user-friendly tables and visualizations summarizing the features of identified motifs and designed sgRNAs. Experimental validation showed that sgRNAs designed by transCRISPR efficiently disrupted MYC binding sites and affected the expression of MYC-regulated genes.
NUCLEIC ACIDS RESEARCH
(2023)
Review
Oncology
Fenneke Zwierenga, Bianca A. M. H. van Veggel, Anke Van den Berg, Harry J. M. Groen, Lili Zhang, Matthew R. Groves, K. Kok, E. F. Smit, T. Jeroen N. Hiltermann, Adrianus J. de Langen, Anthonie J. Van der Wekken
Summary: This article provides a comprehensive assessment of drug sensitivity in vitro and in clinical settings for EGFRex20+ mutations in NSCLC. The activating A763_Y764insFQEA mutation shows better tumor response compared to mutations in other regions, suggesting the need for different treatment approaches. Classification beyond exon-based categories is necessary due to marked differences in treatment responses among EGFRex20+ mutations.
CANCER TREATMENT REVIEWS
(2023)
Article
Biotechnology & Applied Microbiology
Marta Kazimierska, Aleksandra Lesniewska, Anja Bakker, Arjan Diepstra, Marta Elzbieta Kasprzyk, Marta Podralska, Karolina Rassek, Joost Kluiver, Anke van den Berg, Natalia Rozwadowska, Agnieszka Dzikiewicz-Krawczyk
Summary: This study found that GCLC plays an important role in Burkitt lymphoma, and inhibiting GCLC can decrease the viability of BL cells. Buthionine sulfoximine has a growth-inhibiting effect on BL cells and can enhance the cytotoxicity of doxorubicin and cyclophosphamide.
JOURNAL OF APPLIED GENETICS
(2023)
Letter
Oncology
Nick Veltmaat, Yujie Zhong, Filipe Montes de Jesus, Geok Wee Tan, Johanna A. A. Bult, Martijn M. Terpstra, Pim G. N. J. Mutsaers, Wendy B. C. Stevens, Rogier Mous, Joost S. P. Vermaat, Martine E. D. Chamuleau, Walter Noordzij, Erik A. M. Verschuuren, Klaas Kok, Joost L. Kluiver, Arjan Diepstra, Wouter J. Plattel, Anke Van den Berg, Marcel Nijland
Summary: The study investigates the use of cfDNA for genomic profiling of PTLD, and explores its potential as a screening tool. Analysis showed that 41% of patients had EBV-positive tumors and 76% had stage IV disease. The study provides important insights into the genetic variations and EBV infection in PTLD.
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2023)
Correction
Oncology
Sonja I. Berndt, Joseph Vijai, Yolanda Benavente, Nicola J. Camp, Alexandra Nieters, Zhaoming Wang, Karin E. Smedby, Geffen Kleinstern, Henrik Hjalgrim, Caroline Besson, Christine F. Skibola, Lindsay M. Morton, Angela R. Brooks-Wilson, Lauren R. Teras, Charles Breeze, Joshua Arias, Hans-Olov Adami, Demetrius Albanes, Kenneth C. Anderson, Stephen M. Ansell, Bryan Bassig, Nikolaus Becker, Parveen Bhatti, Brenda M. Birmann, Paolo Boffetta, Paige M. Bracci, Paul Brennan, Elizabeth E. Brown, Laurie Burdett, Lisa A. Cannon-Albright, Ellen T. Chang, Brian C. H. Chiu, Charles C. Chung, Jacqueline Clavel, Pierluigi Cocco, Graham Colditz, Lucia Conde, David V. Conti, David G. Cox, Karen Curtin, Delphine Casabonne, Immaculata De Vivo, Arjan Diepstra, W. Ryan Diver, Ahmet Dogan, Christopher K. Edlund, Lenka Foretova, Joseph F. Fraumeni, Attilio Gabbas, Herve Ghesquieres, Graham G. Giles, Sally Glaser, Martha Glenn, Bengt Glimelius, Jian Gu, Thomas M. Habermann, Christopher A. Haiman, Corinne Haioun, Jonathan N. Hofmann, Theodore R. Holford, Elizabeth A. Holly, Amy Hutchinson, Aalin Izhar, Rebecca D. Jackson, Ruth F. Jarrett, Rudolph Kaaks, Eleanor Kane, Laurence N. Kolonel, Yinfei Kong, Peter Kraft, Anne Kricker, Annette Lake, Qing Lan, Charles Lawrence, Dalin Li, Mark Liebow, Brian K. Link, Corrado Magnani, Marc Maynadie, James McKay, Mads Melbye, Lucia Miligi, Roger L. Milne, Thierry J. Molina, Alain Monnereau, Rebecca Montalvan, Kari E. North, Anne J. Novak, Kenan Onel, Mark P. Purdue, Kristin A. Rand, Elio Riboli, Jacques Riby, Eve Roman, Gilles Salles, Douglas W. Sborov, Richard K. Severson, Tait D. Shanafelt, Martyn T. Smith, Alexandra Smith, Kevin W. Song, Lei Song, Melissa C. Southey, John J. Spinelli, Anthony Staines, Deborah Stephens, Heather J. Sutherland, Kaitlyn Tkachuk, Carrie A. Thompson, Herve Tilly, Lesley F. Tinker, Ruth C. Travis, Jenny Turner, Celine M. Vachon, Claire M. Vajdic, Anke Van den Berg, David J. Van den Berg, Roel C. H. Vermeulen, Paolo Vineis, Sophia S. Wang, Elisabete Weiderpass, George J. Weiner, Stephanie Weinstein, Nicole Wong Doo, Yuanqing Ye, Meredith Yeager, Kai Yu, Anne Zeleniuch-Jacquotte, Yawei Zhang, Tongzhang Zheng, Elad Ziv, Joshua Sampson, Nilanjan Chatterjee, Kenneth Offit, Wendy Cozen, Xifeng Wu, James R. Cerhan, Stephen J. Chanock, Susan L. Slager, Nathaniel Rothman
Article
Oncology
Marta Kazimierska, Marta Podralska, Magdalena Zurawek, Tomasz Wozniak, Marta Elzbieta Kasprzyk, Weronika Sura, Wojciech Losiewski, Iwona Ziolkowska-Suchanek, Joost Kluiver, Anke van den Berg, Natalia Rozwadowska, Agnieszka Dzikiewicz-Krawczyk
Summary: The transcription factor MYC plays a crucial role in the development and maintenance of various types of cancer by binding to specific E-box sequences in the genome to regulate gene expression. Through the use of CRISPR/Cas9 technology and high-throughput screening, this study identified essential MYC-regulated genes involved in pathways associated with cancer development. This research provides a novel approach to explore MYC-dependent vulnerabilities in cancer cells, which is of significant importance.
MOLECULAR ONCOLOGY
(2023)
Article
Oncology
Johanna A. A. Bult, Francien Huisman, Yujie Zhong, Nick Veltmaat, Joost Kluiver, Sanne H. Tonino, Joost S. P. Vermaat, Martine E. D. Chamuleau, Arjan Diepstra, Anke van den Berg, Wouter J. Plattel, Mirian Brink, Marcel Nijland
Summary: This population-based study reveals the clinical characteristics associated with transformation and survival after transformation of marginal zone lymphoma (tMZL). Elevated LDH and nodal MZL subtype at MZL diagnosis increase the risk of transformation, while radiotherapy reduces the risk. Age >60 years and (immuno)chemotherapy before transformation are associated with an increased risk of relapse and mortality. Two-year progression-free survival and overall survival are higher for R-(mini)CHOP-treated tMZL patients.
BLOOD CANCER JOURNAL
(2023)