Article
Chemistry, Multidisciplinary
Teodora Andrian, Pietro Delcanale, Silvia Pujals, Lorenzo Albertazzi
Summary: The functionalization of nanoparticles with functional moieties is important for achieving cell targeting in nanomedicine. The correlation between size and ligand number in nanoparticles is crucial for understanding structure-activity relations. A new method combining super-resolution microscopy and transmission electron microscopy, called super-resCLEM, has been introduced to accurately measure these parameters and address the issue of heterogeneity in nanoparticle populations.
Review
Chemistry, Physical
Peter D. Dahlberg, W. E. Moerner
Summary: srCryoCLEM is an emerging method that combines the advantages of super-resolution fluorescence microscopy and cryogenic electron tomography, providing higher resolution and more informative data. The method is still in its infancy with many technical challenges to be overcome.
ANNUAL REVIEW OF PHYSICAL CHEMISTRY, VOL 72
(2021)
Article
Cell Biology
D. Lumkwana, C. Peddie, J. Kriel, L. L. Michie, N. Heathcote, L. Collinson, C. Kinnear, B. Loos
Summary: The study reveals that spermidine induces autophagic flux in a concentration-dependent manner, but the detectable change in the autophagy response critically depends on the specificity and sensitivity of the method employed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Yicong Wu, Xiaofei Han, Yijun Su, Melissa Glidewell, Jonathan S. Daniels, Jiamin Liu, Titas Sengupta, Ivan Rey-Suarez, Robert Fischer, Akshay Patel, Christian Combs, Junhui Sun, Xufeng Wu, Ryan Christensen, Corey Smith, Lingyu Bao, Yilun Sun, Leighton H. Duncan, Jiji Chen, Yves Pommier, Yun-Bo Shi, Elizabeth Murphy, Sougata Roy, Arpita Upadhyaya, Daniel Colon-Ramos, Patrick La Riviere, Hari Shroff
Summary: Researchers have enhanced the performance of confocal microscopy using four approaches: developing compact line scanners, combining multi-view imaging, adapting techniques from structured illumination microscopy, and utilizing deep learning. They successfully improved imaging resolution, reduced phototoxicity, and demonstrated these advancements on over 20 different samples.
Article
Optics
Luis E. Villegas-Hernandez, Vishesh Dubey, Mona Nystad, Jean-Claude Tinguely, David A. Coucheron, Firehun T. Dullo, Anish Priyadarshi, Sebastian Acuna, Azeem Ahmad, Jose M. Mateos, Gery Barmettler, Urs Ziegler, Asa Birna Birgisdottir, Aud-Malin Karlsson Hovd, Kristin Andreassen Fenton, Ganesh Acharya, Krishna Agarwal, Balpreet Singh Ahluwalia
Summary: This study introduces a photonic chip as a high-throughput microscopy platform for super-resolution imaging of histological samples. It demonstrates diverse imaging capabilities and paves the way for the adoption of super-resolution high-throughput multimodal analysis of cryopreserved tissue samples.
LIGHT-SCIENCE & APPLICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Anish R. Roy, Wei Zhang, Zeinab Jahed, Ching-Ting Tsai, Bianxiao Cui, W. E. Moerner
Summary: The topography of the plasma membrane has a strong influence on cellular processes. Recent studies have used 3D nanostructures to study the effects of membrane curvature on protein distribution. In this study, a method combining silicone-oil immersion objective and 3D double-helix point spread function microscopy is introduced to investigate the nanoscale distribution of proteins at the nano-bio interface.
Article
Multidisciplinary Sciences
Chenxi Qian, Kun Miao, Li-En Lin, Xinhong Chen, Jiajun Du, Lu Wei
Summary: VISTA is a label-free superresolution method combining sample expansion and vibrational imaging, enabling high-resolution interrogation of protein-rich biological structures in cells and tissues, with a resolution down to 78 nm.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Dokyung Jeong, Doory Kim
Summary: The combination of correlative super-resolution fluorescence microscopy (SRM) and electron microscopy (EM) is an effective hybrid technique that allows the simultaneous acquisition of spatial locations of specific molecules and the context of cellular ultrastructure. Although there are challenges in integrating these two microscopy techniques, extensive research has led to significant improvements and widespread applications.
MOLECULES AND CELLS
(2022)
Article
Multidisciplinary Sciences
Jing Wang, Steven Randolph, Qian Wu, Aurelien Botman, Jenna Schardt, Cedric Bouchet-Marquis, Xiaolin Nan, Chad Rue, Marcus Straw
Summary: A CLEM workflow based on a novel FIB/SEM technology using an OSSM process was proposed, which achieved high resolution and imaging efficiency while improving throughput. By combining with iPALM technique, successful 3D imaging of mitochondria was carried out, demonstrating the advantages of OSSM-SEM in CLEM applications.
SCIENTIFIC REPORTS
(2021)
Review
Optics
Mingwei Tang, Yubing Han, Danchen Jia, Qing Yang, Ji-Xin Cheng
Summary: Far-field chemical microscopy, with molecular electronic or vibrational fingerprint information, offers a new perspective for studying three-dimensional biological, material, and chemical systems. Despite the limitation of optics, recent advances in super-resolution techniques have overcome this hurdle, pushing the boundaries of far-field chemical microscopy in terms of spatial resolution. This technique finds applications in various fields including biomedical research, material characterization, environmental study, cultural heritage conservation, and integrated chip inspection.
LIGHT-SCIENCE & APPLICATIONS
(2023)
Article
Biochemical Research Methods
Linjing Fang, Fred Monroe, Sammy Weiser Novak, Lyndsey Kirk, Cara R. Schiavon, Seungyoon B. Yu, Tong Zhang, Melissa Wu, Kyle Kastner, Alaa Abdel Latif, Zijun Lin, Andrew Shaw, Yoshiyuki Kubota, John Mendenhall, Zhao Zhang, Gulcin Pekkurnaz, Kristen Harris, Jeremy Howard, Uri Manor
Summary: Point-scanning imaging systems are widely used for high-resolution cellular and tissue imaging, but optimizing resolution, speed, sample preservation, and signal-to-noise ratio simultaneously is challenging. The use of deep learning-based supersampling, known as point-scanning super-resolution (PSSR) imaging, can mitigate these limitations. PSSR facilitates high-resolution, fast, and sensitive image acquisition with otherwise unattainable resolution.
Review
Biochemistry & Molecular Biology
Koen van den Dries, Jack Fransen, Alessandra Cambi
Summary: Correlative light and electron microscopy (CLEM) is a powerful imaging approach that combines light and electron microscopy, and has wide applications in biology. This review summarizes the important developments in CLEM for biological research, with a focus on the combination of fluorescence microscopy and electron microscopy.
Article
Biochemical Research Methods
Biagio Mandracchia, Jeonghwan Son, Shu Jia
Summary: The study introduced an optofluidic scanning microscopy (OSM) system for super-resolution, live-cell imaging, utilizing multi-focal excitation through the innate fluidic motion of specimens to achieve effective resolution doubling, optical sectioning, and contrast enhancement. The system features minimal instrumental complexity and full compatibility with various microfluidic configurations, offering a promising super-resolution optofluidic paradigm for miniaturization and different levels of integration at the chip scale.
News Item
Multidisciplinary Sciences
Ewen Callaway
Summary: The technique surpasses the resolving power of multi-million-dollar machines.
Article
Biochemistry & Molecular Biology
Ivona Kubalova, Alzbeta Nemeckova, Klaus Weisshart, Eva Hribova, Veit Schubert
Summary: Fluorescence light microscopy is important for studying cellular structures, but limited by light diffraction; super-resolution techniques like SIM are used to overcome this limitation; however, STED and SMLM, which offer higher resolution, are not widely applied in plant cell research due to specific sample preparation requirements.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Clinical Neurology
Claudia Pisanu, Giovanni Severino, Ilario De Toma, Mara Dierssen, Paolo Fusar-Poli, Massimo Gennarelli, Pietro Lio, Elisabetta Maffioletti, Eduard Maron, Divya Mehta, Alessandra Minelli, Marie-Claude Potier, Alessandro Serretti, David Stacey, Roos van Westrhenen, Laura Xicota, Bernhard T. Baune, Alessio Squassina
Summary: The study focused on investigating the expression levels of genes involved in drug disposition and action in tissues of pharmacological importance in patients treated with psychotropic drugs. Promising findings include the role of genes in inflammation, neurotransmission, and mitochondrial function. However, studies on antipsychotics or mood stabilizers often had smaller sample sizes and lacked replication compared to studies on antidepressants.
EUROPEAN NEUROPSYCHOPHARMACOLOGY
(2022)
Article
Clinical Neurology
Laura Xicota, Ilario De Toma, Elisabetta Maffioletti, Claudia Pisanu, Alessio Squassina, Bernhard T. Baune, Marie Claude Potier, David Stacey, Mara Dierssen
Summary: Pharmacotranscriptomics is a promising field that utilizes transcriptomic studies to predict treatment response in neurological and psychiatric disorders. This review provides an overview of the biological foundations, methodological approaches, and potential markers and sources of RNA in pharmacotranscriptomic studies. It also discusses the suitability and indications of different techniques and provides recommendations for future directions.
EUROPEAN NEUROPSYCHOPHARMACOLOGY
(2022)
Article
Dermatology
Maher Abou Karam, Ekaterina Mukhina, Nils Daras, Isabelle Rivals, Helene Pillet, Wafa Skalli, Nathanael Connesson, Yohan Payan, Pierre-Yves Rohan
Summary: This study proposed a bedside protocol combining ultrasound and shear wave elastography, tested on healthy young and older subjects. Results showed good reliability with room for improvement.
JOURNAL OF TISSUE VIABILITY
(2022)
Article
Gastroenterology & Hepatology
Pierre Lapaquette, Amandine Ducreux, Louise Basmaciyan, Tracy Paradis, Fabienne Bon, Amandine Bataille, Pascale Winckler, Bernhard Hube, Christophe d'Enfert, Audrey Esclatine, Elisabeth Dubus, Marie-Agnes Bringer, Etienne Morel, Frederic Dalle
Summary: This study investigated the role of autophagy-related proteins in the infection of epithelial cells by Candida albicans. The results showed that autophagy-related proteins were recruited to invasion sites and autophagosomes were formed during active penetration of C. albicans into host cells. These autophagic events were associated with plasma membrane damage. Additionally, the autophagy-related proteins ATG5 and ATG16L1 played a crucial role in maintaining plasma membrane repair and protecting epithelial cells against C. albicans-induced cell death.
Editorial Material
Cell Biology
Pierre Lapaquette, Amandine Ducreux, Etienne Morel, Frederic Dalle
Summary: This study demonstrates the protective role of autophagy in epithelial cells during Candida albicans infection by repairing cell membrane damage, maintaining cell integrity, and preventing cell death.
Correction
Cell Biology
Caterina Miceli, Federica Roccio, Lucille Penalva-Mousset, Martine Burtin, Christine Leroy, Ivan Nemazanyy, Nicolas Kuperwasser, Marco Pontoglio, Gerard Friedlander, Etienne Morel, Fabiola Terzi, Patrice Codogno, Nicolas Dupont
NATURE CELL BIOLOGY
(2022)
Article
Otorhinolaryngology
Valerie Attali, Mathilde Weber, Isabelle Rivals, Thomas Similowski, Isabelle Arnulf, Peggy Gatignol
Summary: This study found that lingual motion is altered during wakefulness in non- or mildly obese patients with moderate-to-severe OSAS. The MBLF assessment can be used to screen for moderate-to-severe OSAS, and this simple tool could assist clinicians in selecting patients for polysomnography.
EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Anthony Vial, Luca Costa, Patrice Dosset, Pietro Rosso, Gaelle Boutieres, Orestis Faklaris, Heiko Haschke, Pierre-Emmanuel Milhiet, Christine M. Doucet
Summary: Nuclear pore complexes (NPCs) are the gateways that control molecular transport between the nucleus and cytoplasm in eukaryotic cells. Recent studies have revealed the arrangement of most constituents in human NPCs using cryo-EM and AI-based protein modeling. However, the structure of the basket, which caps the nucleoplasmic side of the pore, remains poorly resolved. Using atomic force microscopy (AFM) and single molecule localization microscopy (SMLM), researchers discovered that the basket is highly flexible and can adopt different conformations, allowing diverse cargoes to pass through NPCs.
Article
Biology
Juliane Da Graca, Juliette Charles, Morgane Djebar, Karla Alvarez-Valadez, Joelle Botti, Etienne Morel
Summary: Nutrient deprivation, or starvation, is a significant stress that mammalian cells face in various conditions. In this study, the researchers discovered that starvation leads to the connection of endosomal tubules and ER subregions, promoting the formation of autophagosomes. They also found that SNX1 protein is crucial in regulating this endosomal membrane generation and its importance in the autophagic response. The study highlights the contribution of early endosomes in the cellular response to nutritional stress.
LIFE SCIENCE ALLIANCE
(2023)
Article
Clinical Neurology
Anita Sathyanarayanan, Tamara T. Mueller, Mohammad Ali Moni, Katja Schueler, Bernhard T. Baune, Pietro Lio, Divya Mehta
Summary: This review summarizes the methods for discovering biologically meaningful biomarkers for diagnosis, treatment, and prognosis by combining multi-omics data. It discusses conventional and state-of-the-art statistical and machine learning approaches, as well as the role of biological model systems and in silico multi-omics model designs in clinical translation of psychiatric research. Challenges and future applications of multi-omics integration in psychiatric research are also discussed.
EUROPEAN NEUROPSYCHOPHARMACOLOGY
(2023)
Article
Cell Biology
Jack-Christophe Cossec, Tatiana Traboulsi, Sebastien Sart, Yann Loe-Mie, Manuel Guthmann, Ivo A. Hendriks, Ilan Theurillat, Michael L. Nielsen, Maria-Elena Torres-Padilla, Charles N. Baroud, Anne Dejean
Summary: Recent advances in synthetic embryology have revealed that chemical inhibition of SUMOylation in mouse embryonic stem cells (ESCs) can lead to the generation of embryo-like structures. These structures can self-organize into gastrulating structures and undergo axial organization reminiscent of natural embryo morphogenesis. This approach provides a proof of principle for exploring early embryogenesis by targeting chromatin roadblocks of cell fate change.
Article
Psychiatry
Jean M. Delabar, Julien Lagarde, Marta Fructuoso, Ammara Mohammad, Michel Bottlaender, Eric Doran, Ira Lott, Isabelle Rivals, Frederic A. Schmitt, Elizabeth Head, Marie Sarazin, Marie-Claude Potier
Summary: Early markers for effective prevention of Alzheimer's disease are important. Plasma DYRK1A levels were found to be decreased in individuals with Alzheimer's disease compared to controls. DYRK1A levels were inversely correlated with brain amyloid beta burden in asymptomatic elderly individuals and Alzheimer's disease patients, and low levels were also found in patients with tauopathies. Higher DYRK1A levels were observed in individuals with Down syndrome, but lower levels were found in individuals with Down syndrome and dementia. These findings suggest that plasma DYRK1A levels could serve as early detection markers for individuals at risk of Alzheimer's disease.
TRANSLATIONAL PSYCHIATRY
(2023)
Article
Psychiatry
Laura Xicota, Julien Lagarde, Fanny Eysert, Benjamin Grenier-Boley, Isabelle Rivals, Alexandra Botte, Sylvie Forlani, Sophie Landron, Clement Gautier, Cecilia Gabriel, Michel Bottlaender, Jean-Charles Lambert, Mounia Chami, Marie Sarazin, Marie-Claude Potier
Summary: Morphological alterations in the endosomal compartment were observed in post-mortem brains of Alzheimer's disease (AD) patients and Down syndrome (DS) patients who have a high risk of AD. In this study, fibroblasts from a well-characterized cohort of AD patients were analyzed to investigate the association between endosomal compartment and AD. The results revealed an increased volume of EEA1-positive puncta in fibroblasts from AD patients, which correlated with amyloid deposition in the brain and cognitive decline. Genetic risk factors for AD were also identified. In conclusion, the endosomal compartment in fibroblasts may serve as a peripheral cellular biomarker for amyloid deposition and cognitive decline in AD.
TRANSLATIONAL PSYCHIATRY
(2023)