Article
Biotechnology & Applied Microbiology
Xinyue Zhang, Ruling Yang, Mengjie Ma, Siyu Chen, Kaiyin Guo, Li Zhang, Yingnan You, Hailin Jia, Bo Han
Summary: This study investigated the plasma exosome miRNA profile in pediatric fulminant myocarditis (FM) using small RNA microarray. Differential expression of 266 miRNAs was observed, including 197 upregulated and 69 downregulated candidate genes. Through analysis, FM-specific target genes related to inflammation and/or the immune system were identified. CeRNA networks between cardiomyocytes and peripheral blood mononuclear cells were established, and specific miRNAs were validated as potential biomarkers for FM diagnosis.
Article
Biochemistry & Molecular Biology
Yu-Jen Chen, Chia-Tien Hsu, Shang-Feng Tsai, Cheng-Hsu Chen
Summary: Chronic allograft dysfunction (CAD) is a major condition affecting long-term kidney graft survival. In this study, the correlation between plasma miR-21 and the severity of CAD was investigated. RNA sequencing was performed and differentially expressed miRNAs in CAD were identified. MiR-21-5p, miR-20a-5p, and miR-101-3p were found to be associated with the TGF-beta pathway and CAD. These findings may lead to the development of early prediction miRNA biomarkers and therapeutic tools for CAD in the field of kidney transplantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yan Zhuang, Jin Wang, Huihui Li, Yanghui Chen, Chen Chen, Dao Wen Wang
Summary: The study identified plasma Siglec-5 and CD163 as valuable novel biomarkers for the early diagnosis of fulminant myocarditis (FM).
Article
Biochemistry & Molecular Biology
Atefeh Mohammadloo, Yazdan Asgari, Aghil Esmaeili-Bandboni, Mohammad Ali Mazloomi, Seyedeh Fatemeh Ghasemi, Sima Ameri, Seyed Rouhollah Miri, Shahin Hamzelou, Hamid Reza Mahmoudi, Ziba Veisi-Malekshahi
Summary: The study investigated the use of microRNAs as biomarkers for early diagnosis of melanoma. Through analysis of gene expression datasets and PCR experiments, it was found that miR-193b and miR-146b-3p were downregulated in melanoma patients' plasma, while miR-483-3p was upregulated. ROC curve analysis confirmed that miR-193b and miR-146b-3p had high discriminatory power, making them suitable for noninvasive early diagnosis of melanoma.
MOLECULAR BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shalini Das Gupta, Robert Ciszek, Mette Heiskanen, Niina Lapinlampi, Janne Kukkonen, Ville Leinonen, Noora Puhakka, Asla Pitkanen
Summary: The study identified plasma miR-9-3p and miR-136-3p as promising biomarker candidates for mTBI, showing potential for distinguishing mTBI patients with high sensitivity and specificity. Further validation in a larger patient population is needed to confirm their diagnostic utility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Yang Su, Yuxi Sun, Yansong Tang, Hao Li, Xiaoyu Wang, Xin Pan, Weijing Liu, Xianling Zhang, Fenglei Zhang, Yawei Xu, Chunxi Yan, Sang-Bing Ong, Dachun Xu
Summary: The study identified miR-19b-3p as a valuable prognostic biomarker for AHF patients, indicating ventricular hypertrophy and correlating with echocardiographic indexes.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2021)
Article
Medicine, General & Internal
Jingjing Jing, Miao Chang, Shuyi Jiang, Tianlong Wang, Qiuyan Sun, Jun Yang, Chunyan Ma, Tan Li
Summary: The serum level of miR-1-3p was significantly decreased in AAA patients and negatively correlated with inflammatory markers and aneurysm dimensions. MiR-1-3p can be used as a diagnostic biomarker for AAA and plays a crucial role in the development of AAA.
ANNALS OF MEDICINE
(2023)
Article
Oncology
Rocio B. Duca, Cintia Massillo, Guillermo N. Dalton, Paula L. Farre, Karen D. Grana, Kevin Gardner, Adriana De Siervi
Summary: This research identified the association between a high fat diet and prostate cancer, pinpointing the potential importance of two miRNAs in the diagnosis and prognosis of prostate cancer. Further investigations revealed the significant relevance of these miRNAs in distinguishing tumor types and metastatic status.
AMERICAN JOURNAL OF CANCER RESEARCH
(2021)
Article
Food Science & Technology
Da-Bin Hwang, Yoojin Seo, Eunji Lee, Dong-hoon Won, Changuk Kim, MinHwa Kang, Young Jeon, Hyung-Sik Kim, Jun Won Park, Jun-Won Yun
Summary: Evaluation of tissue injury currently relies on serum biochemical analysis, which lacks tissue specificity and sensitivity. MicroRNAs (miRNAs) have been proposed as potential diagnostic tools to overcome these limitations, as tissue-enriched miRNAs can be detected in the blood after tissue injury. In this study, we screened specific patterns of altered hepatic miRNAs and their target mRNAs in cisplatin-injected rats, and identified novel liver-specific circulating miRNAs for drug-induced liver injury. Comparative analyses of miRNA expression changes in organs and serum were conducted, and miR-532-3p was identified as a potential serum biomarker for drug-induced liver injury.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Oncology
Dennis Gerloff, Stefanie Kewitz-Hempel, Gerd Hause, Jovine Ehrenreich, Linda Golle, Tim Kingreen, Cord Sunderkoetter
Summary: Extracellular vesicles (EVs) play an important role in intercellular communication, influencing the function and phenotype of different cell types within the tumor microenvironment and promoting tumor progression. This study identified miRNAs enriched in both melanoma cells and melanoma cell-derived EVs, and showed that these miRNAs were also elevated in serum-derived EVs of patients with metastatic melanoma, suggesting their potential as prognostic or diagnostic liquid biopsy markers in melanoma.
FRONTIERS IN ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Enoch Kang, Sung Cheol Jung, Soo Kyung Nam, Yujun Park, Soo Hyun Seo, Kyoung Un Park, Heung-Kwon Oh, Duck-Woo Kim, Sung-Bum Kang, Hye Seung Lee
Summary: This study evaluated the prognostic potential of EMT-related miRNAs as biomarkers in colorectal cancer (CRC). The results showed that the tissue expression of miR-200c-3p and the plasma levels of miR-1290 were associated with poor prognosis.
SCIENTIFIC REPORTS
(2022)
Article
Physiology
Jian Han, Xiaogang Cui, Tianqi Yuan, Zhiming Yang, Yue Liu, Yajuan Ren, Changxin Wu, Yunfei Bian
Summary: Circulating exosomal miRNAs have potential as biomarkers for diagnosis of cardiovascular diseases. In this study, the differentially expressed miRNAs in exosomes from plasma of SCAD patients were analyzed and their diagnostic potential for SCAD was investigated.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Obstetrics & Gynecology
M. Insenser, A. Quintero, S. de Lope, F. Alvarez-Blasco, M. A. Martinez-Garcia, M. Luque-Ramirez, H. F. Escobar-Morreale
Summary: This research found that the expression of miR-142-3p and miR-598-3p, which are associated with polycystic ovary syndrome (PCOS), differs significantly between women with PCOS and those without. These circulating miRNAs, when combined with easily available measurements such as waist-to-hip ratio (WHR) and circulating LH-to-FSH (LH/FSH) ratios, can effectively distinguish between women with and without PCOS.
HUMAN REPRODUCTION
(2023)
Article
Cell Biology
Alireza Bakhshi, Mohammad Khani, Saeed Alipour Parsa, Isa Khaheshi, Mohammad Hassan Namazi, Arash Mazouri, Peyman Bidram, Morteza Safi, Hossein Vakili, Vahid Eslami, Habib Saadat, Laleh Heidari, Nasim Sohrabifar
Summary: The expression levels of miR-17-3p, miR-101-3p, miR-335-3p, miR-296-3p, and NLRP3 were evaluated in STEMI, NSTEMI, and control groups. NLRP3 was upregulated in both STEMI and NSTEMI patients, while miR-17-3p, miR-101-3p, and miR-296-3p were downregulated. MiR-17-3p showed the highest diagnostic power in distinguishing STEMI patients from controls.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Geriatrics & Gerontology
Jiao Wang, Wenxin Qi, Hongwei Shi, Lin Huang, Fujiang Ning, Fushuai Wang, Kai Wang, Haotian Bai, Hao Wu, Junyi Zhuang, Huanle Hong, Haicong Zhou, Hu Feng, Yinping Zhou, Naijun Dong, Li Liu, Yanyan Kong, Jiang Xie, Robert Chunhua Zhao
Summary: This study used a bioinformatics approach to identify differentially expressed genes in schizophrenia patients and found that miR-4763-3p regulates the downregulation of RASD2. The study also identified potential key brain regions in schizophrenia patients and discovered that inhibition of miR-4763-3p can alleviate symptoms. These findings suggest that miR-4763-3p may serve as a potential biomarker and therapeutic target for schizophrenia.
Article
Cardiac & Cardiovascular Systems
Wu He, Gang Yuan, Yu Han, Yongcui Yan, Gen Li, Chengcheng Zhao, Jingshan Shen, Xiangrui Jiang, Chen Chen, Li Ni, Dao Wen Wang
Summary: Long-term continuous use of glimepiride is associated with better survival, fewer hospitalizations and emergency visits for heart failure, and fewer hospitalizations for acute myocardial infarction or stroke. High-dose glimepiride has greater cardiovascular protective advantages than low-dose glimepiride. The cardiovascular protective effect of glimepiride may be related to the increase in epoxyeicosatrienoic acid levels through soluble epoxide hydrolase inhibition.
EUROPEAN JOURNAL OF PREVENTIVE CARDIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Jianing Gao, Yanghui Chen, Huiqing Wang, Xin Li, Ke Li, Yangkai Xu, Xianwei Xie, Yansong Guo, Nana Yang, Xinhua Zhang, Dong Ma, Hong S. Lu, Ying H. Shen, Yong Liu, Jifeng Zhang, Y. Eugene Chen, Alan Daugherty, Dao Wen Wang, Lemin Zheng
Summary: This study investigates the role of GSDMD in vascular smooth muscle cell phenotypic alteration and abdominal aortic aneurysm (AAA) formation. Gsdmd is found to be upregulated in aortic VSMCs in Ang II-induced AAA. VSMC-specific Gsdmd deletion ameliorates AAA formation. Untargeted metabolomic analysis reveals a significant reduction of putrescine in GSDMD-deficient mice.
Review
Biology
Xiang Nie, Jiahui Fan, Dao Wen Wang
Summary: Cardiac fibrosis is a significant issue in cardiovascular diseases, with molecular mechanisms involving TGF-beta signaling, extracellular matrix synthesis and degradation, and non-coding RNAs. Long noncoding RNAs play a crucial role in myocardial fibrosis and can regulate fibrosis by affecting miRNA expression, TGF-beta signaling, and ECM synthesis. Understanding these mechanisms can guide the development of novel therapeutic strategies to prevent or reverse cardiac fibrosis.
Article
Biochemistry & Molecular Biology
Jiali Nie, Yu Han, Zhiyuan Jin, Weijian Hang, Hongyang Shu, Zheng Wen, Li Ni, Dao Wen Wang
Summary: Editing MYBPC3 gene using CRISPR/Cas9 system in vivo can rescue the phenotype of rats with hypertrophic cardiomyopathy. The editing corrected some mutations, restored MYBPC3 protein expression, and normalized the HCM phenotype.
Article
Cardiac & Cardiovascular Systems
Chen Chen, Wu He, Dao Wen Wang
Summary: In November 2019, Wuhan became the epicenter of the COVID-19 outbreak caused by the novel SARS-CoV-2 infection. The emergence of new strains and mutations of the virus posed a serious global health threat. COVID-19-associated cardiac injury, ranging from mild cases to fulminant myocarditis, is not uncommon, and the overactivated immune response and inflammatory storm play a crucial role in its pathogenesis. Early identification and diagnosis are crucial for the treatment of COVID-19-associated myocarditis.
Article
Oncology
Hong Yang, Rui Li, Fei Ma, Yujian Liu, Xingwei He, Qiao Yang, Dao Wen Wang, Hesong Zeng, Hong Wang
Summary: This study aimed to explore and determine whether echocardiographic phenotype provides value for further risk stratification and guiding treatment in AL patients with heart failure. The results showed that right ventricular wall thickness (RVT), relative wall thickness (RWT), and left ventricular ejection fraction (LVEF) < 50% were independently associated with survival. Survival was significantly reduced in the presence of RVT >= 6.5 mm or LVEF < 50%. Echocardiographic phenotype has incremental value beyond rMayo staging for predicting survival and guiding treatment in AL patients with heart failure.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Medicine, General & Internal
Jiang He, Nanxiang Ouyang, Xiaofan Guo, Guozhe Sun, Zhao Li, Jianjun Mu, Dao Wen Wang, Lixia Qiao, Liying Xing, Guocheng Ren, Chunxia Zhao, Ruihai Yang, Zuyi Yuan, Chang Wang, Chuning Shi, Songyue Liu, Wei Miao, Guangxiao Li, Chung-Shiuan Chen, Yingxian Sun
Summary: This study tested the effectiveness of a non-physician community health-care provider-led intervention for hypertension. The results showed that this intervention can effectively reduce the risk of cardiovascular disease and death.
Review
Biology
Wu He, Ling Zhou, Ke Xu, Huihui Li, James Jiqi Wang, Chen Chen, DaoWen Wang
Summary: Myocarditis is an inflammatory cardiac disease that causes the destruction of myocardial cells, infiltration of inflammatory cells, and fibrosis, and is becoming a major public health concern. The aetiology of myocarditis is expanding with the emergence of new pathogens and drugs. The relationship between immune checkpoint inhibitors, severe acute respiratory syndrome coronavirus 2, vaccines against coronavirus disease-2019, and myocarditis is gaining attention. Immunopathological processes play a significant role in the development and prognosis of myocarditis. Excessive immune activation can cause severe myocardial injury, while chronic inflammation can result in cardiac remodelling and dilated cardiomyopathy. The use of immunosuppressive treatments, particularly cytotoxic agents, for myocarditis remains controversial, and immunomodulatory therapy is the general trend. This review focuses on the current understanding of the aetiology and immunopathogenesis of myocarditis and provides new perspectives on immunomodulatory therapies.
SCIENCE CHINA-LIFE SCIENCES
(2023)
Article
Medicine, Research & Experimental
Jiabing Zhan, Kunying Jin, Nan Ding, Yufei Zhou, Guo Hu, Shuai Yuan, Rong Xie, Zheng Wen, Chen Chen, Huaping Li, Dao Wen Wang
Summary: Intensive glycemic control is insufficient for reducing the risk of heart failure in patients with diabetes mellitus. MiR-320 and CD36 mutually enhance each other's expression, forming a positive feedback loop that sustains a hyperlipidemic state in the heart.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Medicine, Research & Experimental
Rong Xie, Jiahui Fan, Jianpei Wen, Kunying Jin, Jiabing Zhan, Shuai Yuan, Yuyan Tang, Xiang Nie, Zheng Wen, Huaping Li, Chen Chen, Dao Wen Wang
Summary: Diabetes can directly cause cardiac injury, leading to cardiomyopathy. In this study, the protective role of ZNF593-AS in diabetic cardiomyopathy was identified. ZNF593-AS attenuated cardiomyocyte apoptosis and inflammation by suppressing the phosphorylation and activation of IRF3, suggesting that ZNF593-AS may be a potential therapeutic target for diabetic cardiomyopathy.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Oncology
Jiaqi Dai, Tao Wang, Ke Xu, Yang Sun, Zongzhe Li, Peng Chen, Hong Wang, Dongyang Wu, Yanghui Chen, Lei Xiao, Hao Liu, Haoran Wei, Rui Li, Liyuan Peng, Ting Yu, Yan Wang, Zhongsheng Sun, Dao Wen Wang
Summary: Previous studies have identified differences in symptom severity and prognosis among patients with hypertrophic cardiomyopathy (HCM), suggesting the presence of potential HCM subtypes. In this study, consensus clustering was performed on echocardiography features of 793 HCM patients to identify potential subtypes. Machine learning modeling and interactome network detection techniques were used to explore the relationship between phenotype and genotype based on whole-exome sequencing data. Two subtypes were identified: subtype 2 characterized by stable course with asymmetric septal hypertrophy, and subtype 1 characterized by aggressive progression and left ventricular systolic dysfunction. Machine learning modeling based on personal whole-exome data accurately predicted subtype propensities using 46 genes with high mutation burden. Another cohort replicated the findings, showing increased left ventricular end-diastolic diameter and reduced ejection fraction in patients predicted as subtype 1. Echocardiography and genetic screening for the 46 genes can be used to classify HCM into two subtypes with distinct clinical outcomes.
FRONTIERS OF MEDICINE
(2023)
Review
Pharmacology & Pharmacy
Zuowen He, Xin Xu, Qu Zhao, Hu Ding, Dao Wen Wang
Summary: This article summarizes the characteristics, etiology, diagnosis, and treatment of vasospastic angina (VSA), with a focus on the role of inflammation. It provides new insights into the understanding of VSA.
PHARMACOLOGY & THERAPEUTICS
(2023)
Article
Cell Biology
Huihui Li, Mingzhi Zhang, Quanyi Zhao, Wanqing Zhao, Yan Zhuang, Jin Wang, Weijian Hang, Zheng Wen, Li Wang, Chen Chen, Dao Wen Wang
Summary: This study reveals the pathogenesis of fulminant myocarditis (FM) and suggests potential therapeutic strategies. Healthy cardiomyocytes lose their function and differentiate into pro-angiogenic and pro-inflammatory cells in FM. Neutrophils, after migrating to the heart, continuously attract peripheral neutrophils via specific signals, resulting in their acute accumulation in the heart. Moreover, neutrophils can amplify the inflammatory response by recruiting and activating pro-inflammatory cells. Blockade of the self-recruiting loop of neutrophils substantially alleviates FM in mice.
Article
Biochemistry & Molecular Biology
Yanru Zhao, Huaping Li, Hengzhi Du, Zhongwei Yin, Mengying He, Jiahui Fan, Xiang Nie, Yang Sun, Huiying Hou, Beibei Dai, Xudong Zhang, Yuanyuan Cai, Kunying Jin, Nan Ding, Zheng Wen, Jiang Chang, Chen Chen, Dao Wen Wang
Summary: This study found that KSHV infection is a risk factor for dilated cardiomyopathy, and the KSHV-encoded miRNA kshv-miR-K12-1-5p may be involved in the development of heart disease.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)