Article
Microbiology
Jin-Feng Chen, Yu Liu, Gui-Rong Tang, Dan Jin, Xi Chen, Yan Pei, Yan-Hua Fan
Summary: The study revealed that BbSmr1 plays a critical role in the regulation of asexual conidiation development and stress response in B. bassiana, with its deficiency leading to various abnormal phenotypes and functions. The gene is associated with BbbrlA and BbabaA, promoting conidial development by regulating the central developmental pathway.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Review
Plant Sciences
Panchsheela Nogia, Pratap Kumar Pati
Summary: The study focuses on the important roles of secondary metabolites (SMs) in plant growth, defense, development, and survival, as well as explores the transport mechanisms of SMs and the potential of engineering transporters to enhance metabolite production.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Farzaneh Darigh, Alireza Iranbakhsh, Zahra Oraghi Ardebili, Mostafa Ebadi, Halimeh Hassanpour
Summary: In vitro plant culture using simulated microgravity has been found to increase the production and concentration of cannabinoids in Cannabis indica callus cells, which has significant implications for meeting industrial demand for medicinal cannabis.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Biology
Valentin Gfeller, Jan Waelchli, Stephanie Pfister, Gabriel Deslandes-Herold, Fabio Mascher, Gaetan Glauser, Yvo Aeby, Adrien Mestrot, Christelle A. M. Robert, Klaus Schlaeppi, Matthias Erb
Summary: Plant secondary metabolites released into the rhizosphere impact soil properties, affecting the performance and productivity of other plants. In this study, winter wheat varieties showed improved growth and yield when grown in soils conditioned by maize plants producing benzoxazinoids. This finding suggests that optimizing root exudation chemistry can enhance crop yields without additional inputs.
Article
Plant Sciences
Yixian Lin, Hui Zhang, Peirong Li, Juan Jin, Zhefei Li
Summary: The study found that using bacterial consortia inoculation can increase the root weight of Astragalus mongholicus, improve the content of medicinal components, and maintain these effects under drought conditions.
Article
Multidisciplinary Sciences
Ann-Katrin Beuel, Natalia Jablonka, Julia Heesel, Kevin Severin, Holger Spiegel, Stefan Rasche
Summary: The LEDitSHAKE system allows for multiplexed customized illumination within a single shaking incubator, enabling testing of 12 different lighting conditions simultaneously. A proof of principle using the system to optimize anthocyanin production in grapevine cell suspension cultures showed that short-wavelength light (blue, UV) maximized the concentration of most anthocyanins.
SCIENTIFIC REPORTS
(2021)
Article
Plant Sciences
Amir Ali, Zia-ur-Rehman Mashwani, Naveed Iqbal Raja, Sher Mohammad, Juan Pedro Luna-Arias, Ajaz Ahmad, Prashant Kaushik
Summary: This study aims to develop an in vitro elicitation strategy to enhance the biomass and production of secondary metabolites in Caralluma tuberculata through nano elicitors and light regimes.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Izabela Grzegorczyk-Karolak, Katarzyna Hnatuszko-Konka, Marta Krzeminska, Monika A. Olszewska, Aleksandra Owczarek
Summary: The study focuses on exploring the organogenic competence of various growth regulators to develop an efficient protocol for in vitro regeneration of Salvia bulleyana via leaf explants, aiming to maintain high production of active constituents and assess the possibilities of producing therapeutic compounds. Both direct and indirect organogenesis were observed, with the highest regeneration frequency achieved on leaves cultured in specific growth medium. The genetic stability of the obtained organogenic shoot lines was confirmed through ISSR marker-based profiling, indicating potential for stable cultures rich in bioactive metabolites.
Article
Agriculture, Multidisciplinary
Dongmei Lyu, Rachel Backer, Fabrice Berrue, Camilo Martinez-Farina, Joseph P. M. Hui, Donald Lawrence Smith
Summary: Inoculating plant growth-promoting rhizobacteria (PGPR) in cannabis plants can enhance yield attributes and chemical profiles. When inoculated with Bacillus sp., Mucilaginibacter sp., and Pseudomonas sp. at the vegetative stage, cannabis showed increased flower dry weight, total CBD, and THC. Inoculation with Mucilaginibacter sp. and Pseudomonas sp. at the flowering stage led to increased total terpene accumulation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Engineering, Biomedical
Jesus Romo-Rico, Smriti Murali Krishna, Kateryna Bazaka, Jonathan Golledge, Mohan Jacob
Summary: There is a global epidemic of non-healing wounds, and plant secondary metabolites (PSMs) have shown potential in improving wound healing through their antioxidant, anti-inflammatory, and antibacterial properties. This review focuses on PSMs-based polymers as wound dressings and discusses their potential clinical value in aiding wound healing.
ACTA BIOMATERIALIA
(2022)
Article
Forestry
Ge Guo, Byoung Ryong Jeong
Summary: The Korean fir is a unique tree species in Korea, but many have died due to climate change. This study investigated callus induction using different explants, media, and plant growth regulators. It was found that stem segments were more suitable for callus induction, with the Murashige and Skoog medium being the most effective and PGR combinations containing 2,4-D showing positive impact on callus induction and proliferation.
Review
Plant Sciences
Kai Hao, Yun Wang, Zhanpin Zhu, Yu Wu, Ruibing Chen, Lei Zhang
Summary: This review summarizes the recent progress on the contributions of the miR160-ARF module to important traits of plants and the role of miR160-centered gene regulatory network in coordinating growth with endogenous and environmental factors. Manipulation of miR160-guided gene regulation may provide a new method to engineer plants with improved adaptability and yield.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biotechnology & Applied Microbiology
Sushma Mishra, Pramod Kumar Sahu, Vishad Agarwal, Namrata Singh
Summary: Plant secondary metabolites have potential applications in various industries, but their direct isolation from plants may lead to over-harvesting and loss of biodiversity. Endophytic microbes have the ability to produce metabolites similar to those of their host plants, providing a sustainable and commercially valuable resource.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Izabela Weremczuk-Jezyna, Katarzyna Hnatuszko-Konka, Liwia Lebelt, Izabela Grzegorczyk-Karolak
Summary: This study found that LED light sources have a significant impact on the growth and morphology of in vitro cultivated Dracocephalum forrestii shoot culture, with white LED showing the most beneficial proliferation effect. Different light spectra induced stress conditions also led to an increase in the synthesis of pharmacologically active compounds.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Forestry
Aoshun Zhao, Xingcui Ding, Manchang Huang, Yingjie Cheng
Summary: This study isolated 253 different endophytic bacteria strains from Moso bamboo shoots and evaluated their plant growth promoting traits. Among them, Pseudomonas rhodesiae RD7-4 had the strongest phosphorus solubilizing ability, Burkholderia pyrrocinia BD24-2 had the strongest nitrogen fixing ability, and Pseudomonas edaphica TD33-1 had the strongest IAA production ability. Inoculation with these three strains significantly increased the content of soluble starch in Arabidopsis, and B. pyrrocinia BD24-2 significantly increased the fresh weight and promoted the growth of the root system. In pot experiments, B. pyrrocinia BD24-2 also significantly increased the biomass, root length, and leaf area.
Review
Chemistry, Multidisciplinary
Sandeep Kumar Verma, Ashok Kumar Das, Saikat Gantait, Yogita Panwar, Vinay Kumar, Marian Brestic
Summary: Nanomaterials are increasingly used in various fields such as medicine, agriculture, and industry, with carbon-based nanomaterials being a major type with diverse applications. These materials are synthesized in large quantities through synthetic and biological approaches, with biological methods gaining popularity due to their environmentally friendly nature.
Review
Biotechnology & Applied Microbiology
Sima Taheri, Saikat Gantait, Parisa Azizi, Purabi Mazumdar
Summary: This review article highlights the recent progress in improving drought tolerance in tomatoes through omics and multi-omics technologies, including genetic engineering. The discovery of drought-responsive genes and the development of biotechnological tools have provided wider opportunities for the development of drought-tolerant varieties.
Review
Biotechnology & Applied Microbiology
Tsama Subrahmanyeswari, Saikat Gantait
Summary: This review provides an overview of the biotechnological research advances in banana, highlighting various approaches such as in vitro regeneration, genetic transformation, mutagenesis, and nanotechnological and omics techniques. It identifies research gaps, unexplored areas, and major shortcomings in banana biotechnology, and proposes potential strategies to address these issues.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Agronomy
Eashan Mukherjee, Saikat Gantait
Summary: Sugar beet is a commercially important crop due to its rich carbohydrate content. Conventional cultivation practices expose this crop to various stresses, but genetically transformed crops offer a potential alternative. Genetic modification methods such as Agrobacterium spp. and direct transformation methods can improve sugar beet's resistance to diseases, pests, and abiotic stresses, as well as enhance the production of industrially important carbohydrates. The study of genetic engineering in sugar beet since the early 1990s has explored different transformation techniques and their potential applications in improving the crop's tolerance to biotic and abiotic stresses.
Article
Agronomy
Tsama Subrahmanyeswari, Saroj Laha, Suchita N. Kamble, Sudhir Singh, Somnath Bhattacharyya, Saikat Gantait
Summary: An accelerated, unique and efficient alginate-encapsulation-based mass propagation system was developed for stevia, which exhibited accelerated germination, higher shoot proliferation rate and elongation, and quicker root initiation and multiplication. Synthetic seed-regenerated plantlets showed clonal fidelity with their mother plant. This protocol is important for germplasm exchange and production of stevia.
Review
Plant Sciences
Saikat Gantait, Tsama Subrahmanyeswari, Manisha Mahanta
Summary: Aloin, a derivative of anthraquinone found in Aloe vera, has various applications in the cosmeceutical industry. This review focuses on aloin chemistry, biosynthesis, cosmeceutical applications, and biotechnological approaches for aloin production. It highlights the research gaps, unexplored areas, and major obstacles in enhancing aloin production.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Article
Plant Sciences
Manisha Mahanta, Saikat Gantait, Epsita Mukherjee, Somnath Bhattacharyya
Summary: This study reports the in vitro mass propagation of Gerbera jamesonii Bolus ex Hooker f., a highly demanded cut-flower in the floriculture industry, induced by meta-Topolin (mT). The subsequent acclimatization process and cyto-genetic fidelity assessment of the plantlets were also carried out. Different cytokinins and cytokinin-like plant growth regulators were supplemented in the basal Murashige and Skoog (MS) medium, and the growth performance of the shoot tip explants was compared. The developed protocol for large-scale production of true-to-type planting materials of gerbera is of great commercial importance.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Article
Biotechnology & Applied Microbiology
Manisha Mahanta, Saikat Gantait, Sutanu Sarkar, Raghunath Sadhukhan, Somnath Bhattacharyya
Summary: An efficient in vitro protocol for high-frequency polyploidization in gerbera hybrid was developed in this study. The protocol involved treating two-week-old shoots with colchicine, followed by multiple shoot proliferation and rooting. The tetraploid plants obtained exhibited unique morphological characteristics and improved genetic traits, which are of great importance for the genetic improvement of gerbera.
Review
Biotechnology & Applied Microbiology
Himanshi Swain, Saikat Gantait, Nirmal Mandal
Summary: In the search for new medications, researchers have been studying nature to discover beneficial plant species that possess medicinal qualities for curing various diseases and disorders. These medicinal plants produce different bioactive secondary metabolites, including reserpine, which has been used for centuries to treat hypertension, cardiovascular diseases, neurological diseases, breast cancer, and human promyelocytic leukaemia. This review comprehensively discusses non-conventional or in vitro-mediated biotechnological methods for the production of reserpine from Rauvolfia spp., such as multiple shoot culture, callus culture, cell suspension culture, precursor feeding, elicitation, synthetic seed production, scale-up via bioreactor, and hairy root culture. The review also explores the potential of unexplored and cutting-edge biotechnological tools and techniques in increasing reserpine production.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Agricultural Engineering
Saroj Laha, Tsama Subrahmanyeswari, Sandeep Kumar Verma, Suchita N. Kamble, Sudhir Singh, Somnath Bhattacharyya, Saikat Gantait
Summary: In this study, a simple and eco-friendly method for synthesizing silver nanoparticles (AgNPs) using leaf extract of in vitro banana plantlets was established. The synthesized AgNPs were characterized and then used to study their effects on the growth and rebaudioside-A content of stevia under in vitro conditions. The results showed that 400 μM AgNPs concentration in 'semi-solid followed by liquid' medium exhibited the best growth response and highest rebaudioside-A production.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Biotechnology & Applied Microbiology
Monalisha Char, Tsama Subrahmanyeswari, Somnath Bhattacharyya, Saikat Gantait
Summary: This study successfully established a large-scale in vitro shoot, microtuber, and root development protocol for potato cv. Badami alu, and confirmed its genetic stability using various analytical methods.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Plant Sciences
Saikat Gantait, Sangata Das, Manisha Mahanta, Maitreyi Banerjee
Summary: This study for the first time investigated the impact of in vitro culture age on Rauvolfia serpentina, and found that it affected the morphology, antioxidant activities, and reserpine production. The study showed that the shoot length and internode lengths were significantly different among different cultures. The antioxidant activities were reduced in the in vitro culture compared to the ex vitro plantlet, but the reserpine production increased in the in vitro culture. The study also revealed a certain level of genetic variation between the in vitro culture and the ex vitro plantlet.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Article
Plant Sciences
Tsama Subrahmanyeswari, Saikat Gantait, Suchita N. Kamble, Sudhir Singh, Somnath Bhattacharyya
Summary: This study found that the use of meta-Topolin (mT) in vitro can promote the mass propagation of stevia (Stevia rebaudiana Bert.) more effectively than other cytokinins. Adding 1.5 mg/L mT to the culture medium resulted in the highest number and length of shoots and a greater number of leaves. The acclimatized plantlets exhibited proper growth, stable ploidy level, and higher rebaudioside-A production compared to the mother plant.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Article
Agronomy
Tsama Subrahmanyeswari, Saikat Gantait, Suchita N. Kamble, Sudhir Singh, Somnath Bhattacharyya
Summary: This study assesses the sensitivity of stevia to gamma irradiation and determines the optimum dosage (15-20 Gy) for inducing beneficial mutations. This dosage can lead to a higher percentage of beneficial mutations and maximize the desirable genetic diversity in M1V2 and subsequent generations.