Journal
TOXINS
Volume 12, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/toxins12080475
Keywords
zearalenone; decontamination; enzymatic degradation; lactonohydrolase; enzyme preparations; recombinant proteins; safe agricultural products; improving the feed nutritional value
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Funding
- Russian Science Foundation [19-76-10031]
- RSF [14-16-00150]
- Russian Science Foundation [19-76-10031, 14-16-00150] Funding Source: Russian Science Foundation
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This paper reports the first results on obtaining an enzyme preparation that might be promising for the simultaneous decontamination of plant feeds contaminated with a polyketide fusariotoxin, zearalenone (ZEN), and enhancing the availability of their nutritional components. A novel ZEN-specific lactonohydrolase (ZHD) was expressed in aPenicillium canescensstrain PCA-10 that was developed previously as a producer of different hydrolytic enzymes for feed biorefinery. The recombinant ZHD secreted by transformed fungal clones into culture liquid was shown to remove the toxin from model solutions, and was able to decontaminate wheat grain artificially infected with a zearalenone-producingFusarium culmorum. The dynamics of ZEN degradation depending on the temperature and pH of the incubation media was investigated, and the optimal values of these parameters (pH 8.5, 30 degrees C) for the ZHD-containing enzyme preparation (PR-ZHD) were determined. Under these conditions, the 3 h co-incubation of ZEN and PR-ZHD resulted in a complete removal of the toxin from the model solutions, while the PR-ZHD addition (8 mg/g of dried grain) to flour samples prepared from the infected ZEN-polluted grain (about 16 mu g/g) completely decontaminated the samples after an overnight exposure.
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