Article
Pharmacology & Pharmacy
Silvia Pisani, Valeria Mauri, Erika Negrello, Valeria Friuli, Ida Genta, Rossella Dorati, Giovanna Bruni, Stefania Marconi, Ferdinando Auricchio, Andrea Pietrabissa, Marco Benazzo, Bice Conti
Summary: In this study, hybrid scaffolds were developed as a versatile platform for soft tissue regeneration. These scaffolds exhibited favorable cell adhesion and proliferation, and allowed for controlled drug release.
Article
Chemistry, Multidisciplinary
Ugo D'Amora, Alfredo Ronca, Stefania Scialla, Alessandra Soriente, Paola Manini, Jun Wei Phua, Christoph Ottenheim, Alessandro Pezzella, Giovanna Calabrese, Maria Grazia Raucci, Luigi Ambrosio
Summary: The study developed a photocrosslinkable biomaterial ink, methacrylated GG (GGMA), by chemically modifying gellan gum (GG) with methacrylic moieties. GGMA was then functionalized with bioactive compounds such as black soldier fly eumelanin (BSF-Eumel) or hydroxyapatite nanoparticles (HAp). Different ink formulations were used to 3D print scaffolds, and the ones with higher GGMA concentration and percentage of infill showed the best morphological and mechanical properties. The scaffolds demonstrated the capability to support cell growth and differentiation, with BSF-Eumel promoting cell proliferation and GGMA and HAp leading to higher alkaline phosphatase activity expression. Overall, these biomaterial inks have potential for 3D-printed bone tissue-engineered scaffolds.
Review
Chemistry, Multidisciplinary
Motaharesadat Hosseini, Abbas Shafiee
Summary: Large skin wounds present challenges in clinical settings, with a wide variety of skin grafts and scaffolds being used to overcome issues related to tissue repair. Progress in biomaterial science and manufacturing technologies has made the idea of biomaterial-based skin grafts with scarless wound healing and full skin organ reconstruction more achievable than ever.
Review
Biotechnology & Applied Microbiology
Sebastian Loewner, Sebastian Heene, Timo Baroth, Henrik Heymann, Fabian Cholewa, Holger Blume, Cornelia Blume
Summary: Melt electro writing (MEW) is a high-resolution 3D printing technique that deposits micro- to nanometer strands of biocompatible polymers layer by layer, making it a promising method for tissue engineering scaffold fabrication. This review discusses optimization strategies for precise fiber deposition, printing protocols for different scaffold structures, and the combination of MEW with other fabrication techniques to create advanced scaffold structures.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Aipeng Deng, Yang Yang, Shimei Du
Summary: Electrospun recombinant human collagen (RHC)/chitosan nanofibers combined with freeze-dried porous scaffolds were successfully fabricated into 3D nanofibrous scaffolds with high porosity and interconnected porous structure. These scaffolds exhibited good swelling ability, stability, and mechanical strength, showing promising potential for tissue engineering applications.
APPLIED SCIENCES-BASEL
(2021)
Article
Materials Science, Multidisciplinary
Mehdi Karimi, Azadeh Asefnejad, Dorsa Aflaki, A. Surendar, Hadi Baharifar, Saeed Saber-Samandari, Amirsalar Khandan, Afrasyab Khan, Davood Toghraie
Summary: This study used 3D printing and freeze-drying techniques to manufacture porous scaffolds, and through the study of mechanical and biological properties, it was found that the sample with 20wt% porosity exhibited excellent strength and biocompatibility. The conclusion indicates that the produced bio-nanocomposite scaffolds have potential application in tissue engineering.
JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
(2021)
Article
Otorhinolaryngology
Hanaro Park, In Gul Kim, Yanru Wu, Hana Cho, Jung-Woog Shin, Su A. Park, Eun-Jae Chung
Summary: After evaluating esophageal reconstructions using different scaffolds, it was found that 3DP PCL scaffolds had better muscle regeneration while PU-Nf scaffolds had better epithelial regeneration.
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
(2021)
Article
Biochemistry & Molecular Biology
Sayan Deb Dutta, Jin Hexiu, Dinesh K. Patel, Keya Ganguly, Ki-Taek Lim
Summary: Hybrid biodegradable hydrogels composed of alginate, gelatin, and cellulose nanocrystals were 3D printed to create an optimal environment for bone tissue engineering applications. The scaffolds exhibited enhanced mechanical strength and bone regeneration potential compared to pure polymer scaffolds. The study demonstrated improved cell proliferation, adhesion, mineralization, and expression of osteogenic genes in the presence of the printed scaffolds.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Engineering, Biomedical
Pengfei Li, Liming Ruan, Guohua Jiang, Yanfang Sun, Ruofan Wang, Xiaofei Gao, Khaydar E. Yunusov, Uladzislau E. Aharodnikau, Sergey O. Solomevich
Summary: Electrospun nanofibrous scaffolds have great potential for wound healing as they effectively mimic the extracellular matrix and possess various advantages such as high specific surface areas, good mimicry, adjustable properties, histocompatibility, and promoting wound healing. This study designed a 3D nanofibrous scaffold that incorporated gelatin, heparin, and vascular endothelial growth factors, exhibiting antibacterial ability, hemocompatibility, and promoting angiogenesis and wound healing.
ACTA BIOMATERIALIA
(2022)
Review
Pharmacology & Pharmacy
Amber F. MacDonald, Meaghan E. Harley-Troxell, Steven D. Newby, Madhu S. Dhar
Summary: This article summarizes the application of graphene-based materials in tissue engineering, with a focus on bone tissue engineering. It discusses the need for developing 3D graphene scaffolds and introduces the current manufacturing techniques and limitations.
Article
Chemistry, Physical
Ghazal Belgheisi, Masoumeh Haghbin Nazarpak, Mehran Solati-Hashjin
Summary: This study presents a new approach to produce tissue engineering scaffolds by combining electrospinning and additive manufacturing technologies. The fabricated 3D-structured scaffolds consist of pamidronate-loaded layered double hydroxides/polycaprolactone electrospun nanofibers and micro-strand polycaprolactone 3D printed grids. The scaffolds show reduced fiber diameter and increased surface roughness when pamidronate-loaded layered double hydroxides are added to polycaprolactone. The final structure of the scaffolds is obtained by gluing the nanofiber mats between 3D printed micro-strand grids. The scaffolds demonstrate suitable pore size, controllable drug release, and enhanced cell adhesion and osteoconductivity.
APPLIED CLAY SCIENCE
(2022)
Article
Materials Science, Ceramics
Wenpu Zhang, Xinghui Hou, Huili Wang, Dezhi Kong, Ying Zhou
Summary: A chitosan-sodium alginate/bioactive glass (CSB) composite cartilage scaffold with outstanding in vitro mineralization property and cytocompatibility was synthesized by freeze drying method. The effect of bioactive glass addition on the microstructure, porosity, swelling/degradation ratio, in vitro mineralization property and cytocompatibility of CSB scaffold was investigated. The results showed that the CSB composite scaffold had a 3D porous structure and met the requirements of cartilage tissue repair. The scaffold also exhibited good in vitro mineralization property and promoted cell proliferation.
CERAMICS INTERNATIONAL
(2023)
Article
Polymer Science
Evangelos Daskalakis, Boyang Huang, Cian Vyas, Anil Ahmet Acar, Ali Fallah, Glen Cooper, Andrew Weightman, Bahattin Koc, Gordon Blunn, Paulo Bartolo
Summary: This study proposes a method for preparing polycaprolactone/bioglass scaffolds with pore size gradient by investigating the coupled effect between material composition and scaffold architecture. The results show that the addition of bioglass improves the mechanical properties and biological performance of the scaffolds.
Article
Engineering, Biomedical
Liang Yang, Ismat Ullah, Keda Yu, Wancheng Zhang, Jinge Zhou, Tingfang Sun, Lei Shi, Sheng Yao, Kaifang Chen, Xianglin Zhang, Xiaodong Guo
Summary: The innovative gradient SrFeHA/PCL scaffolds exhibit significant bioactivity and immune modulation, promoting bone regeneration and vascularization effectively, thus providing a promising alternative for future bone tissue engineering.
Review
Polymer Science
Elisa Capuana, Francesco Lopresti, Francesco Carfi Pavia, Valerio Brucato, Vincenzo La Carrubba
Summary: The review paper discusses various scaffold fabrication methods starting from polymer solutions that provide highly porous structures, such as freeze-drying, thermally or diffusion induced phase separation, and electrospinning. The combination of these techniques offers the advantage of mimicking the unique architecture of tissues and organs that are intrinsically difficult to design.
Article
Chemistry, Multidisciplinary
Johar Amin Ahmed Abdullah, Mercedes Jimenez Rosado, Antonio Guerrero, Alberto Romero
Summary: This study aims to investigate the green synthesis of ZnO-NPs using a polyphenol-rich extract from Phoenix dactylifera L. as a reducing agent. The effects of precursor and reducing agent concentration were evaluated, and it was found that using a green reducing agent resulted in ZnO-NPs with a particle size ranging from 18.1 to 61.6 nm. The synthesized nanoparticles demonstrated antioxidant and antibacterial activities. Therefore, this method could be a suitable alternative to toxic methods for metallic nanoparticle synthesis.
NEW JOURNAL OF CHEMISTRY
(2023)
Article
Polymer Science
Hanaa Mehdi-Sefiani, Victor Perez-Puyana, Francisco Jose Ostos, Ranier Sepulveda, Alberto Romero, Mohammed Rafii-El-Idrissi Benhnia, Ernesto Chicardi
Summary: A gelatin-based hydrogel was used to infiltrate and degrade-release in two different titanium foams with porosities of 30 and 60 vol.%, showing higher infiltration rate in the Ti60 foam and faster degradation rate in the Ti30 foam. The hydrogel also exhibited optimal biocompatibility with no cytotoxicity and promotion of cell growth.
Article
Polymer Science
Victor M. Perez-Puyana, Antonio J. Capezza, William R. Newson, Carlos Bengoechea, Eva Johansson, Antonio Guerrero, Mikael S. Hendeqvist
Summary: The functionalization of keratin from poultry feathers was evaluated using one- versus two-stage processes with two functionalization agents (succinic anhydride-SA and ethylene dianhydride-EDTAD). The functionalization improved liquid swelling capacities of keratin, with up to 400% water, 300% saline, and 85% blood uptake compared to non-functionalized keratin. One-stage functionalization with EDTAD showed better performance and resource-saving advantages over two-stage processes, making it a potential liquid absorbent material.
Article
Food Science & Technology
Mercedes Jimenez-Rosado, Victor Perez-Puyana, Alberto Romero
Summary: Climate change, waste accumulation, microplastics generation, and raw material scarcity have led to a shift from a linear economic system to a circular system. Policies like the European Green Deal and Horizon Europe have been developed in response. This opinion review discusses the use of biowaste for superabsorbent material production as an example of circular economy application, highlighting limitations that have hindered market inclusion. While biowaste utilization is essential for change, further research is needed to enhance product competitiveness.
CURRENT OPINION IN FOOD SCIENCE
(2023)
Article
Chemistry, Physical
Johar Amin Ahmed Abdullah, Mercedes Jimenez-Rosado, Antonio Guerrero, Alberto Romero
Summary: Nowadays, antioxidants and antibacterial activity have become increasingly important in biosystems due to biochemical and biological reactions involving free radicals and pathogen growth. Nanomaterials are being used as antioxidants and bactericidal agents to minimize these reactions. However, there is a lack of understanding about the antioxidant and bactericidal capacities of iron oxide nanoparticles. This study aimed to evaluate the influence of calcination, specifically different temperatures and times, on the synthesis of iron oxide nanoparticles and their properties.
Article
Polymer Science
Daniel Castro-Criado, Octavio Rivera-Flores, Johar Amin Ahmed Abdullah, Elia Castro-Osorto, Maria Alonso-Gonzalez, Lucy Ramos-Casco, Victor M. Perez-Puyana, Marlon Sanchez-Barahona, Pablo Sanchez-Cid, Mercedes Jimenez-Rosado, Alberto Romero
Summary: The development of biodegradable plastics and eco-friendly biomaterials from agricultural waste and rejected food is important for reducing environmental damage. This research investigated the fabrication and characterization of bioplastics using three types of Honduran agro-wastes: taro, yucca, and banana. The results showed the potential of these agro-wastes for producing bioplastics with different characteristics, promoting the circular economy.
Article
Ecology
Emre Yemisken, Mercedes Jimenez-Rosado, Victor Perez-Puyana, Serap Sancar, Suna Bektas, Taner Yildiz, Lutfiye Eryilmaz, Alberto Romero
Summary: The aim of this study was to isolate and characterize collagen from the skin of round goby (Neogobius melanostomus) in the Black Sea. The results showed that the skin of N. melanostomus had a high protein concentration (80%) and contained characteristic molecules of collagen protein, such as glycine, alanine, proline, and hydroxyproline. The yield of collagen was estimated at 10%. The collagen had a zero surface net charge at pH 5-6, indicating its proximity to the collagen isoelectric point. It also had a highly organized helical structure, which was preserved in round goby species. The biological assays demonstrated that the collagen obtained from round goby fish skin was noncytotoxic and showed an increase in cell viability. These findings suggest that round goby species could be a potential marine source of collagen.
REGIONAL STUDIES IN MARINE SCIENCE
(2023)
Article
Chemistry, Physical
G. Gonzalez-Ulloa, M. Jimenez-Rosado, M. Rafii-El-Idrissi Benhnia, A. Romero, E. Ruiz-Mateos, F. J. Ostos, V. Perez-Puyana
Summary: In the field of regenerative medicine, hydrogels are widely recognized as a suitable option for tissue regeneration due to their elastic properties and high water absorption capacity. This study aimed to develop and characterize polymeric hydrogels based on collagen and gelatin, both of which are considered good candidates due to their biocompatibility. The results showed that hydrogels formed from the mixture of collagen and gelatin retained the viscoelastic properties of collagen while demonstrating low cytotoxicity and hemocompatibility similar to gelatin. However, further research is needed to address the suboptimal thermal characteristics for biomedical applications.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Chemistry, Multidisciplinary
Johar Amin Ahmed Abdullah, Alvaro Diaz-Garcia, Jia Yan Law, Alberto Romero, Victorino Franco, Antonio Guerrero
Summary: This study investigates the impact of pH levels and washing solvents on magnetism properties of chemical nanomagnetic iron oxide particles (CNMIOPs) synthesized through a chemical method. By employing different pH levels and washing solvents, the researchers found that pH and washing process significantly affect the properties of CNMIOPs, with higher pH levels resulting in smaller particles with higher crystallinity. Ethanol-washed CNMIOPs exhibit superior magnetic behavior, and higher pH levels show promising antioxidant activity.
Article
Materials Science, Multidisciplinary
Johar Amin Ahmed Abdullah, Jose J. Benitez, Antonio Guerrero, Alberto Romero
Summary: This study investigated the impact of adding zinc oxide nanoparticles (ZnO-NPs) to poly(epsilon-caprolactone) (PCL) membranes and films. The results showed that adding ZnO-NPs improved the water barrier properties, optical properties, and mechanical properties of the PCL samples, as well as increasing the thickness and surface roughness of the samples.
Article
Agriculture, Multidisciplinary
Mercedes Jimenez-Rosado, Michele Di Foggia, Serena Rosignoli, Antonio Guerrero, Adamo Domenico Rombola, Alberto Romero
Summary: This study investigates the use of controlled-release protein-based matrices for massive crops like barley. By analyzing different barley cultivars and using protein-based matrices as fertilizers, it is found that this method can improve seed germination and zinc content in plants.
RENEWABLE AGRICULTURE AND FOOD SYSTEMS
(2023)
Article
Food Science & Technology
Daniel Castro-Criado, Mercedes Jimenez-Rosado, Victor Perez-Puyana, Alberto Romero
Summary: The production of functional foods has been improved with nanoemulsion technology, which involves the encapsulation of bioactive compounds in beverages to control their integrity and release in the intestinal tract. In this study, a protein-rich by-product was evaluated as a potential emulsifier, and emulsions with different soy protein isolate concentrations, pH values, and homogenization pressures were prepared. Physical and rheological characterizations were conducted to determine their stability and potential as delivery emulsion systems. The best stability was achieved when the protein concentration was 2.0 wt%, pH 2.0, and a homogenization pressure of 120 PSI was applied.
