Study of interactions between blood plasma proteins and poly(butyl cyanoacrylate) drug nanocarriers by surface plasmon resonance.

Investigation and novel data on the interactions between blood plasma proteins and polysorbate 80 coated poly(butyl cyanoacrylate) (PBCA) drug nanocarriers determined by surface plasmon resonance (SPR) are reported. For that purpose, human blood plasma proteins (albumin, immunoglobulin G and fibrinogen) were immobilized on the surface of SPR sensorchips and the kinetics of nanoparticle interaction (adsorption/desorption) with the chip surface was studied at 37 degrees C at pH 7.4. The proposed methodology was found suitable for measurement of interactions of both drug-free and drug-loaded nanocarriers with blood plasma proteins and allowed quantitative evaluation of both rate and equilibrium constants of interactions. Presented results demonstrated that SPR can be considered as a promising technique for real time investigation of both kinetic and equilibrium parameters of the nanoparticle-protein interactions, which could be of importance for the development of drug nanocarrier systems and their biomedical evaluation and application. (C) 2016 Elsevier B.V. All rights reserved.