Article
Agriculture, Dairy & Animal Science
Santina Di Bella, Vincenza Cannella, Francesco Mira, Patrizia Di Marco, Antonio Lastra, Francesca Gucciardi, Giuseppa Purpari, Annalisa Guercio
Summary: The study demonstrated that a high percentage of serum (80%) is necessary for optimal cryopreservation with 10% DMSO. Thawed cells at passages from P2 to P4, even after seven years, could still be considered for therapeutic application and in vitro study as they retain stem potential post-cryopreservation.
Review
Biochemistry & Molecular Biology
Pietro Gentile, Simone Garcovich
Summary: The skin acts as a natural barrier against UV radiation, but prolonged exposure to sunlight can lead to structural changes and photoaging. Adipose-derived mesenchymal stem cells and fat grafting have been utilized to combat signs of photoaging, such as wrinkles and pigmentation, through cellular activity and antioxidant effects.
Article
Biochemistry & Molecular Biology
Nadav Kislev, Roza Izgilov, Raizel Adler, Dafna Benayahu
Summary: Adipose tissue is a complex organ composed of different cellular populations, and changes in these cells can lead to metabolic complications and insulin resistance. The interaction between immune cells and adipocytes is important for tissue regulation.
Review
Oncology
Zoi Karagiorgou, Panagiotis N. Fountas, Dimitra Manou, Erik Knutsen, Achilleas D. Theocharis
Summary: Proteoglycans play important roles in various processes of cancer cells, such as migration, invasion, and drug resistance. They influence cell plasticity, stemness, and alter cellular shape and functions through different mechanisms.
Article
Cell & Tissue Engineering
Lingling Chen, Morgan Carlton, Xiaodan Chen, Navdeep Kaur, Hollie Ryan, Tony J. Parker, Zhengmei Lin, Yin Xiao, Yinghong Zhou
Summary: This study investigated the pluripotency and multilineage differentiation of bone marrow-derived MSCs by introducing FN, FGF2, and BMP4, and identified the metabolic and proteomic cues involved in stemness maintenance. The results showed that the culture environment supplemented with these factors significantly improved BMSC stemness and revealed the importance of metabolism and proteome networks in cell-fate decision making and stemness preservation.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemical Research Methods
Mengchang Liu, Defu Xie, Huizhen Zeng, Ning Zhai, Lan Liu, Hong Yan
Summary: A customized bioreactor was used to stimulate adipose-derived mesenchymal stem cells with electric field (EF) signals. The study revealed that EF stimulation can enhance cell proliferation, providing a unique perspective on adipose-derived mesenchymal stem cell proliferation for tissue engineering and regenerative medicine.
Article
Cell Biology
Maria Serena Piccinno, Tiziana Petrachi, Marco Pignatti, Alba Murgia, Giulia Grisendi, Olivia Candini, Elisa Resca, Valentina Bergamini, Francesco Ganzerli, Alberto Portone, Ilenia Mastrolia, Chiara Chiavelli, Ilaria Castelli, Daniela Bernabei, Mara Tagliazucchi, Elisa Bonetti, Francesca Lolli, Giorgio De Santis, Massimo Dominici, Elena Veronesi
Summary: This study investigates the use of adipose-derived mesenchymal stromal/stem cells (AD-MSCs) in autologous fat transfer (AFT) for tissue reconstruction. The results show that AD-MSCs can improve graft persistence and reduce tissue resorption after AFT. The implanted AD-MSCs can persist and differentiate into mature adipocytes, leading to better outcomes in terms of graft weight and reduced cyst formation. This suggests a long-term protective and anti-inflammatory/proangiogenic role of AD-MSCs in autologous AFT tissue resorption.
Article
Biochemistry & Molecular Biology
Xiao Jiang, Xin-Rui Lai, Jin-Qiang Lu, Ling-Zhi Tang, Jin-Rong Zhang, Hong-Wei Liu
Summary: DAT interacts with various types of MSCs to achieve adipose regeneration. It provides an inductive microenvironment for adipogenesis and supports the expression of PPARg. The presence of multiple adipogenic proteins in DAT plays a vital role in adipose regeneration.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Payal Arora, Wen Li, Xiaobin Huang, Wenjing Yu, Ranran Huang, Qian Jiang, Chider Chen
Summary: This study shows that curcumin can enhance the function of periodontal ligament derived stem cells (PDLSC) and increase their immunomodulatory capacity through activation of specific metabolic pathways. It provides a new pharmacological approach to activate endogenous stem cells for immunomodulation and tissue regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Michiko Horiguchi, Yuko Okada, Yuya Turudome, Kentaro Ushijima
Summary: Type 1 diabetes mellitus leads to degeneration of organelles in adipose-derived stem cells, with the main characteristic being prominent enlarged spherical vesicles. The exosomes of T1DM ADSCs are found to be enlarged, reduced in number, and increased in the percentage positive for tetraspanin CD9, providing insight into the characteristics of stem cells in patients with type 1 diabetes mellitus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Surgery
Pietro Gentile, Valerio Cervelli, Domenico De Fazio, Claudio Calabrese, Maria Giovanna Scioli, Augusto Orlandi
Summary: This article compares four different methods of AD-SVFs isolation and A-FG purification and reports the in vivo and in vitro results, including fat volume maintenance and AD-SVFs amount. It is found that A-FG enhanced with AD-SVFs obtained by automatic enzymatic digestion showed the highest fat volume maintenance of 63% after 1 year, while the maintenance rates were 52% using centrifugation with filtration, 39% using only centrifugation, and 60% using only filtration. Cell analysis indicated that filtration was the most efficient system among mechanical digestion procedures and resulted in the best volume maintenance in vivo. Enzymatic digestion produced the best number of AD-SVFs and the best fat volume maintenance.
AESTHETIC PLASTIC SURGERY
(2023)
Article
Anatomy & Morphology
Valentina Dapra, Daniela Alotto, Stefania Casarin, Stefano Gambarino, Carlotta Zavatto, Maddalena Dini, Ilaria Galliano, Carlotta Castagnoli, Massimiliano Bergallo
Summary: This study validated a commercially available kit for evaluating the osteogenic, chondrogenic, and adipogenic differentiation ability of human ADSC using real-time rt-QPCR. Suitable markers for trilineage differentiation were identified, and the differentiation potential of ADSC was evaluated. The real-time rt-QPCR protocol using this kit was proven to be effective for in-vitro evaluation of ADSC differentiation ability.
Article
Cell & Tissue Engineering
Rose Triantafillia Psaroudis, Urvashi Singh, Maximilien Lora, Peter Jeon, Abigail Boursiquot, Ursula Stochaj, David Langlais, Ines Colmegna
Summary: This study identifies DPP4 gene expression and CD26 protein abundance as markers of replicative senescence in MSC(AT). MSCs with high CD26 expression have reduced immunopotency, and senolytic treatment can decrease the frequency of CD26(high) MSCs.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Zhe Ji, Jian Wang, Shuai Yang, Shengjun Tao, Caiqi Shen, Hanxiao Wei, Qiang Li, Peisheng Jin
Summary: Graphene oxide (GO) can inhibit the apoptosis of adipose-derived mesenchymal stem cells (Ad-MSCs) induced by high glucose and promote diabetic wound healing. The study found that GO regulates the expression of STK4 and downstream signaling pathways by interacting with Linc00324 and miR-7977.
Review
Cell Biology
Sarah Miethe, Daniel P. Potaczek, Stanislawa Bazan-Socha, Melanie Bachl, Liliana Schaefer, Malgorzata Wygrecka, Holger Garn
Summary: Extracellular vesicles (EVs) have gained attention for their role in intercellular signaling, particularly in the connection between adipose tissue (AT) and the lung in diseases such as obesity-associated asthma and lung cancer-associated cachexia. Studies have shown both pathogenic and protective effects of EVs in these diseases, with AT-derived EVs playing a potential therapeutic role. Further research is needed to better understand the role of EVs in disease development and organ interconnectivity.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Oncology
Chunyan Liu, Yanfei Ma, Ruihuan Wang, Guohong Su
Summary: In this study, the role and potential mechanisms of LINC00987 in acute myeloid leukemia (AML) progression were investigated. It was found that LINC00987 was upregulated in AML patients and cell lines. Knockdown of LINC00987 inhibited proliferation and invasion and promoted apoptosis in AML cells. LINC00987 could bind with IGF2BP2 and promote its expression, and IGF2BP2 overexpression reversed the effects of LINC00987 knockdown on AML cell functions.
Article
Biodiversity Conservation
Wenxian Sun, Xiaoyin Niu, Haowei Teng, Yanfei Ma, Longchao Ma, Yizhou Liu
Summary: The study revealed that eutrophication in Chaihe Reservoir in Kunming, China has worsened, with human activities contributing to increased input of organic carbon, nitrogen, and sulfur into the aquatic environment.
ECOLOGICAL INDICATORS
(2022)
Article
Integrative & Complementary Medicine
Xiaoqiang Shi, Yunhua Wu, Enmeng Li, Li Zhang, Yanfei Ma, Guangbing Wei, Xuqi Li, Shufeng Wang
Summary: This study validated the potential of naringin (NG) to reduce intraperitoneal adhesions in a rat model. High-dose NG significantly reduced inflammatory reactions, oxidative stress, collagen deposition, and fibroblast formation, and improved gastrointestinal dynamics.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2022)
Article
Immunology
Yifan Zhong, Jiahong Cao, Yanfei Ma, Yu Zhang, Jianxin Liu, Haifeng Wang
Summary: Transforming the gut microbiota through fecal microbiota transplantation (FMT) has become an intriguing intervention for various gastrointestinal and non-gastrointestinal disorders. This study investigated the effects of different diets on the gut microbiota post-FMT from donors with different diets. The results showed that different dietary fiber contents paired with FMT from different donors led to distinct patterns of gut microbiota and metabolites, highlighting the importance of precise dietary interventions post-FMT.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Energy & Fuels
Hanyang You, Hongyang Lin, Yang Li, Yang Yang, Yanfei Ma, Zhenxiao Shang, Xiaoyin Niu
Summary: In this study, Fe-Al/CSHB and Al/CSTB composites were prepared by impregnation method as sorbents for the removal of NO3-N in rare earth wastewater. The effects of adsorbent dosage, pH, contact time, and temperature on the adsorption performance were investigated. The results showed that Fe-Al/CSHB and Al/CSTB exhibited good adsorption performance for NO3-N at pH 1. The adsorption capacity of Fe-Al/CSHB decreased with increasing temperature, while that of Al/CSTB increased. Characterization analysis revealed the formation of FeOOH, Fe2O3, Fe2+, and Al2O3 mixture on the surface of Fe-Al/CSHB, and the presence of Al2O3 on the surface of Al/CSTB.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Oncology
Zhizhai Luo, Binbin Nong, Yanfei Ma, Dalang Fang
Summary: This study aimed to investigate the role of autophagy-related lncRNA in breast cancer and construct a prognostic risk assessment model. The study found that 11 autophagy-related lncRNAs were independently associated with breast cancer prognosis and were related to immune cell infiltration.
ANNALS OF TRANSLATIONAL MEDICINE
(2022)
Article
Nursing
Xixi Li, Haiyan Wang, Yuanchen Wu, Yanfei Ma
Summary: This study investigated risk perception, coping methods, negative emotions, psychological workload, and awareness of social support among nurses during the COVID-19 pandemic in three large general hospitals in Sichuan Province, China. The findings showed that nurses experienced increased negative emotions, with fear being the most common. The psychological workload of nurses in close contact positions with COVID-19 patients was higher than that of nurses in other positions. Family was the major source of social support for nurses.
JOURNAL OF PSYCHOSOCIAL NURSING AND MENTAL HEALTH SERVICES
(2022)
Article
Immunology
Yanfei Ma, Yanghong Li, Pengwei Guo, Jingjie Zhao, Qiang Qin, Jiajia Wang, Zhengfang Liang, Dalong Wei, Zechen Wang, Jiajia Shen, Siyuan He, Qianli Tang, Guanming Lu, Guiling Shi, Lingzhang Meng
Summary: In this study, three endothelial cell subclusters were identified in the tumor microenvironment, and their roles in metastasis were revealed. This has important implications for inhibiting triple-negative breast cancer metastasis.
JOURNAL OF IMMUNOLOGY RESEARCH
(2022)
Article
Materials Science, Multidisciplinary
Lin Jin, Yanfei Ma, Ruiya Wang, Shuo Zhao, Zhishuai Ren, Shengnan Ma, Yupeng Shi, Bin Hu, Yuqi Guo
Summary: This study prepared a double structure system that can promote vascularization and anti-inflammatory effect in wound healing while preventing scars.
MATERIALS TODAY ADVANCES
(2022)
Article
Environmental Sciences
Wenxian Sun, Zike Zhou, Xianwei Yin, Yongping Wang, Haowei Teng, Aiju Liu, Yanfei Ma, Xiaoyin Niu
Summary: Lake sediment records the evolution process of the interaction between human and nature, and it is important to master the lacustrine sedimentation rate for the ecological environment assessment of catchment. By analyzing various parameters in sediment cores, the response of sedimentation rate to environmental evolution can be reconstructed. Cluster analysis revealed three stages of environmental changes, with increasing sedimentation rates over time, indicating continuous exogenous inputs. Pearson correlation analysis and redundancy analysis showed that sedimentation rates were positively correlated with climatic factors, particle size, nutrients and metals mass fractions, elemental ratios, and socioeconomic parameters. Therefore, sedimentation rates can be used to reconstruct the environmental evolution process at a small catchment scale.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Environmental Sciences
Hongyang Lin, Yang Yang, Zhenxiao Shang, Qiuhong Li, Xiaoyin Niu, Yanfei Ma, Aiju Liu
Summary: This work developed an environmentally-friendly soil remediation method using BC and g-C3N4, demonstrating the technical feasibility of remediating petroleum-contaminated soil with BC/g-C3N4. BC/g-C3N4 composites were synthesized for the removal of TPH in soil through adsorption and photocatalysis. The results showed that BC/g-C3N4 exhibited excellent removal efficiency and had potential for application in organic pollutant remediation.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Environmental Sciences
Yuan Zhang, Shaomin Liu, Lisheng Song, Xiang Li, Zhenzhen Jia, Tongren Xu, Ziwei Xu, Yanfei Ma, Ji Zhou, Xiaofan Yang, Xinlei He, Yunjun Yao, Guangcheng Hu
Summary: This study proposes an integrated validation framework for evaluating the accuracy of remotely sensed evapotranspiration (RS_ET) products over different land surfaces. The framework is applied to five widely used RS_ET products in the Heihe River Basin, analyzing their accuracy, spatiotemporal variations, error sources, and uncertainties during the validation process.
Article
Surgery
Dalang Fang, Yanting Li, Yanghong Li, Yongcheng Chen, Qianfang Huang, Zhizhai Luo, Jinghua Chen, Yingjin Li, Zaizhi Wu, Yuanlu Huang, Yanfei Ma
Summary: By analyzing gene expression data of breast cancer patients, we identified 9 immune-related biomarkers that may be used to assess the sensitivity of neoadjuvant chemotherapy in breast cancer patients, and have the potential to predict pathological complete response in these patients.
Article
Environmental Sciences
Wenxian Sun, Xiaoyin Niu, Xianwei Yin, Zhenhao Duan, Liqi Xing, Aiju Liu, Yanfei Ma, Peiling Gao
Summary: This study investigated the heterogeneity of PAHs in the sediment core of Chaihe Reservoir to assess the dynamic changes in pollution levels and sources, and proposed recommendations for controlling PAHs. The results showed an overall decline in PAH pollution in the reservoir, but coal, oil, and natural gas consumption still played significant roles in transferring PAHs. Local sources such as grass, wood, and coal combustion were also identified. The study highlights the importance of both natural and anthropogenic interventions in mitigating PAH pollution.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Agronomy
Chen Zheng, Shaomin Liu, Lisheng Song, Ziwei Xu, Jingxin Guo, Yanfei Ma, Qian Ju, Jiemin Wang
Summary: This study compared and analyzed the sensible (H) and latent (LE) heat fluxes measured by optical-microwave scintillometers (OMS) and eddy-covariance (EC) method. The differences between OMS and EC measurements were approximately 17% and 27% for H and LE over homogeneous and heterogeneous surfaces, respectively. The energy balance closure ratio of OMS system increased by 26% over the heterogeneous surface. These results indicate the potential advantage of OMS in measuring heat fluxes, especially over heterogeneous surfaces.
AGRICULTURAL AND FOREST METEOROLOGY
(2023)
