Article
Oncology
Xuexue Xu, Pengsheng Ding, Lan Shi, Gang Wu, Xiaoling Ma
Summary: In this study, it was found that LukS-PV inhibited HCC cell migration by down-regulating HDAC6 and increasing the acetylation level of alpha-tubulin.
Article
Biochemistry & Molecular Biology
Rushuang Xu, Shan He, Di Ma, Rui Liang, Qing Luo, Guanbin Song
Summary: In this study, the role of plectin in the migration and epithelial mesenchymal transition (EMT) of hepatocellular carcinoma (HCC) cells was investigated. Plectin expression was found to be significantly increased in HCC tissue and cells. Downregulation of plectin inhibited HCC cell migration and EMT, which was mediated by the suppression of ERK1/2 signaling. Activation of ERK1/2 reversed the inhibitory effects of plectin downregulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Nan Zhang, Shaoqin Zhang, Wenda Wu, Wenxian Lu, Mingting Jiang, Ning Zheng, Jing Huang, Long Wang, Hekun Liu, Min Zheng, Jichuang Wang
Summary: Research shows that regorafenib significantly inhibits EMT in HCC cells by targeting ID1, leading to the suppression of cell migration, invasion and VM formation. This suggests that regorafenib may be developed as a suitable therapeutic agent for treating HCC metastasis.
MOLECULAR CARCINOGENESIS
(2021)
Article
Biochemistry & Molecular Biology
Lu Han, Xin Lin, Qun Yan, Chuncai Gu, Mengshu Li, Lei Pan, Yan Meng, Xinmei Zhao, Side Liu, Aimin Li
Summary: PBLD inhibits hypoxia-induced angiogenesis in hepatocellular carcinoma cells through multiple mechanisms, including downregulation of HIF-1a and VEGF expression via the ERK/HIF-1a/VEGF axis, as well as blocking VEGFR2 on endothelial cells through HCC-derived exosomal miR-940.
Article
Medicine, Research & Experimental
Ritesh Urade, Wen-Tsan Chang, Ching-Chung Ko, Ruei-Nian Li, Hui-Min Yang, Hsuan-Yu Chen, Lin-Ya Huang, Meng-Yang Chang, Chang-Yi Wu, Chien-Chih Chiu
Summary: In this study, a novel fluorene derivative, 9-methanesulfonylmethylene-2, 3-dimethoxy-9 H-fluorene (MSDF), was found to have anticancer potential in human hepatocellular carcinoma (HCC) cells. MSDF induced apoptosis through both receptor-mediated extrinsic and mitochondrial-mediated intrinsic pathways. It also caused anoikis and cell death by disrupting cell-ECM contact. MSDF suppressed the MAPK/ERK and PI3K/Akt signaling pathways and downregulated the expression of immune checkpoint proteins.
Article
Integrative & Complementary Medicine
Fulong Nan, Wenlong Nan, Zhongjie Yu, Hui Wang, Xiaoni Cui, Shasha Jiang, Xianjuan Zhang, Jun Li, Zhifei Wang, Shuyun Zhang, Bin Wang, Yiquan Li
Summary: Platycodon grandiflorum is a medicinal plant commonly used in East Asia, and its triterpene saponins have shown anti-tumor effects. In this study, the inhibitory effect of polygalacin D (PGD) on hepatocellular carcinoma cells was investigated. PGD was found to induce apoptosis and autophagy in these cells through the mitochondrial apoptosis and mitophagy pathways. In addition, in vivo experiments confirmed the anti-tumor activity of PGD. These findings suggest that PGD can be developed as a novel anti-tumor agent by targeting the apoptosis and autophagy pathways.
CHINESE JOURNAL OF NATURAL MEDICINES
(2023)
Article
Oncology
Dawei Yuan, Rulan Ma, Tuanhe Sun, Kun Zhu, Chengxue Dang, Haixia Ye, Kang Li
Summary: The high expression of RSPH14 in hepatocellular carcinoma (HCC) is associated with poor prognosis. Knockdown of RSPH14 can inhibit cell proliferation, migration, and invasion, and promote apoptosis of HCC cells by inhibiting the expression of RelA. RSPH14 may be a potential new treatment target for HCC.
CANCER CELL INTERNATIONAL
(2022)
Article
Environmental Sciences
Huang-Ming Hu, Hsiang-Lin Lee, Chung-Jung Liu, Yi-Hsien Hsieh, Yong-Syuan Chen, Kuan-Chun Hsueh
Summary: The study found that MTA2 regulates HCC progression through the MTA2-FAK-MMP7 pathway. PTK7, as a downstream factor of MTA2, affects the migration and invasion abilities of HCC cells by regulating MMP7 expression. The expression level of PTK7 is correlated with tumor stage, grade, and overall survival in HCC patients, and may have diagnostic value for HCC patients.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Medical Laboratory Technology
An Wang, Xiaofeng Chen, Dongen Li, Liang Yang, Jianshuai Jiang
Summary: In this study, we found that ASPM was significantly upregulated in LIHC and its high expression was associated with poor prognosis. Knockdown of ASPM suppressed LIHC cell proliferation, migration, and invasion, while overexpression had the opposite effect. Mechanistically, we revealed that METTL3-mediated N6-methyladenosine modification of ASPM mRNA promoted its expression in LIHC. Silencing METTL3 also inhibited LIHC cell proliferation, migration, and invasion, but this effect could be rescued by overexpression of ASPM. These findings suggest that the METTL3/ASPM axis may be a promising therapeutic target for LIHC.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2021)
Article
Cell Biology
Jinghua Lu, Yipei Ding, Wanqiu Zhang, Yuanyuan Qi, Jin Zhou, Naihan Xu, Yaou Zhang, Weidong Xie
Summary: Clinical analysis indicates that SQSTM1/p62 is highly expressed in HCC and affects patient prognosis. Knocking out SQSTM1/p62 using the CRISPR/Cas9 system impairs HCC migration and invasion, upregulates Keap1, and promotes the inhibitory effect of Keap1 on Nrf2. Inactivation of Nrf2 inhibits MMP expression, attenuating HCC migration and invasion. SQSTM1/p62 knockout also inhibits migration and invasion in a mouse model of lung metastasis. Cisplatin inhibits SQSTM1/p62 expression and slows down HCC migration and invasion, while the inflammatory microenvironment accelerates HCC migration and invasion. This study suggests that SQSTM1/p62 knockout inhibits HCC migration and invasion through the Keap1/Nrf2/MMP2 signaling pathway, highlighting it as a potential drug target.
Article
Multidisciplinary Sciences
Adan Olguin-Olguin, Anne Aalto, Benoit Maugis, Aleix Boquet-Pujadas, Dennis Hoffmann, Laura Ermlich, Timo Betz, Nir S. Gov, Michal Reichman-Fried, Erez Raz
Summary: In studying the mechanisms controlling front-rear polarity in migrating cells using zebrafish primordial germ cells as an in vivo model, it was found that the accumulation and flow of actin plays a key role. Following cell division, the accumulation of Ezrin at the cleavage plane is the first sign for cell polarity. The antagonistic interactions between the cell front and back determine the polarization of the cell.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Oncology
Yue Li, Jiongshan Zhang, Kun Zhang, Yan Chen, Wei Wang, Hongjie Chen, Zengcheng Zou, Yongwei Li, Min Dai
Summary: This study demonstrated that Scutellaria barbata suppresses the growth of hepatocellular carcinoma cells by inducing ferroptosis, potentially by promoting iron perioxidation and lipid ROS metabolism. Additionally, S. barbata can inhibit HCC tumorigenicity in vivo by inducing ferroptosis of HCC cells.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cell Biology
Fei Cao, Anguo Luo, Chaowen Yang
Summary: Ferroptosis is a significant form of cell death in cancer research, with G6PD and POR being associated with both HCC progression and prognosis. G6PD promotes HCC development by inhibiting ferroptosis, while downregulation of POR is correlated with better prognosis in HCC.
CELLULAR SIGNALLING
(2021)
Review
Immunology
Sihang Yu, Lei Zhou, Jiaying Fu, Long Xu, Buhan Liu, Yuanxin Zhao, Jian Wang, Xiaoyu Yan, Jing Su
Summary: There is increasing evidence showing the crucial role of the immune microenvironment, particularly the polarization state and function of macrophages, in the development of hepatocellular carcinoma. Tumor-derived exosomes in hepatocellular carcinoma act as information carriers and regulate the physiological state of cells in the microenvironment, thus controlling cancer progression. This review focuses on the role of exosome content in disease outcomes at different stages of hepatitis B virus/hepatitis C virus-induced hepatocellular carcinoma, and explores the mechanism by which macrophages contribute to hepatocellular carcinoma formation and the regulation of macrophage functions by the heterogeneity of exosome loading. The application prospects of exosome-based targeted drug delivery in immunotherapy research on hepatocellular carcinoma are also summarized.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Toxicology
Yang Liu, Xian-Ji Piao, Wan-Ting Xu, Yu Zhang, Tong Zhang, Hui Xue, Yan-Nan Li, Wen-Bo Zuo, Geng Sun, Zhong-Ren Fu, Ying-Hua Luo, Cheng-Hao Jin
Summary: Calycosin exhibited anti-proliferation effects on HCC cells and had less cytotoxicity in normal cells. It induced apoptosis by regulating various signaling pathways and caused cell cycle arrest in the G0/G1 phase. Additionally, calycosin reduced the expression of certain proteins and induced cellular events through ROS-mediated pathways.
TOXICOLOGY IN VITRO
(2021)
Article
Chemistry, Multidisciplinary
Olanrewaju Ayodeji Durojaye, Divine Mensah Sedzro, Mukhtar Oluwaseun Idris, Abeeb Abiodun Yekeen, Adeola Abraham Fadahunsi, Oluwaseun Suleiman Alakanse
Summary: This study utilized a reverse vaccinology approach to design a nucleic acid vaccine candidate targeting the trimeric spike glycoprotein of SARS-CoV-2. Through antigenicity assessments and bioinformatics analyses, a suitable peptide sequence was selected as the basis for vaccine candidate design, with optimization targeted at TLR7.
Article
Biochemistry & Molecular Biology
Sikai Liu, Xiao Yuan, Ping Gui, Ran Liu, Olanrewaju Durojaye, Donald L. Hill, Chuanhai Fu, Xuebiao Yao, Zhen Dou, Xing Liu
Summary: In mitosis, the interaction between Cyclin B2 and Mad2 guides accurate chromosome segregation through their localization at the kinetochores.
Article
Biochemistry & Molecular Biology
Yaqian Liu, Ruoxing Jin, Hui Lu, Kangjie Bian, Rui Wang, Lei Wang, Rui Gao, Jiahai Zhang, Jihui Wu, Xuebiao Yao, Xing Liu, Dan Liu, Xisheng Wang, Zhiyong Zhang, Ke Ruan
Summary: YEATS family proteins recognize acylated histones and regulate chromatin structure, gene transcription, and stress signaling. In this study, researchers identified inhibitors for the AF9 YEATS domain and designed new inhibitors based on complex structures and affinity assays. These inhibitors showed antiproliferation activities in AF9-sensitive cancer cells. This work provides a basis for further optimization of AF9 YEATS inhibitors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ya Jiang, Ting Wang, Dandan Sheng, Chaoqiang Han, Tian Xu, Peng Zhang, Weiyi You, Weiwei Fan, Zhiyong Zhang, Tengchuan Jin, Xiaotao Duan, Xiao Yuan, Xing Liu, Kaiguang Zhang, Ke Ruan, Jue Shi, Jing Guo, Aoxing Cheng, Zhenye Yang
Summary: This study reveals a new protumor function of Aurora A by phosphorylating PKM2 during the S phase of the cell cycle to promote glycolysis and tumor growth. The spatial and temporal regulation of the specific kinase and PKM2 interaction is context dependent, showing intricate interconnectivity between cell cycle and glycolytic regulators.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Cell Biology
Divine Mensah Sedzro, Xiao Yuan, McKay Mullen, Umer Ejaz, Tongtong Yang, Xu Liu, Xiaoyu Song, Yun-Chi Tang, Weijun Pan, Peng Zou, Xinjiao Gao, Dongmei Wang, Zhikai Wang, Zhen Dou, Xing Liu, Xuebiao Yao
Summary: The study reveals that CENP-R collaborates with CENP-OPQU to regulate the stability of kinetochore-microtubule attachment and ensure accurate chromosome segregation during mitosis. Phosphorylation of CENP-R affects its localization to the kinetochore, possibly by disrupting its binding with CENP-U.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Cell Biology
Hameed Akbar, Jun Cao, Dongmei Wang, Xiao Yuan, Manjuan Zhang, Saravanakumar Muthusamy, Xiaoyu Song, Xu Liu, Felix Aikhionbare, Xuebiao Yao, Xinjiao Gao, Xing Liu
Summary: During cell division, acetylation disrupts the nucleoporin complex and promotes the distribution of Nup62 in the mitotic spindle, ensuring proper spindle orientation and chromosome segregation. Suppression of Nup62 leads to inaccurate chromosome segregation.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Divine Mensah Sedzro, Mukhtar Oluwaseun Idris, Olanrewaju Ayodeji Durojaye, Abeeb Abiodun Yekeen, Adeola Abraham Fadahunsi, Suleiman Oluwaseun Alakanse
Summary: This study aimed to find novel inhibitors of the p53-MDM2 interaction. Through pharmacophore modeling and screening, three compounds with potential therapeutic effects for cancer were identified, which showed favorable interactions with important residues involved in the p53-MDM2 protein-protein interaction.
Article
Cell Biology
Tian Tian, Lili Chen, Zhen Dou, Zhisen Yang, Xinjiao Gao, Xiao Yuan, Chengliang Wang, Ran Liu, Zuojun Shen, Ping Gui, Maikun Teng, Xianlei Meng, Donald L. Hill, Lin Li, Xuan Zhang, Xing Liu, Linfeng Sun, Jianye Zang, Xuebiao Yao
Summary: This study reveals the molecular basis of how human CCAN interacts with duplex DNA and its importance in accurate chromosome segregation through cryo-electron microscopy and functional analyses.
Article
Cell Biology
Yanze Jian, Lingyun Nie, Sikai Liu, Yueyue Jiang, Zhen Dou, Xing Liu, Xuebiao Yao, Chuanhai Fu
Summary: The outer kinetochore acts as a platform for SAC initiation and mediating kinetochore-microtubule attachments. The inner kinetochore subcomplex CENP-S-CENP-X's involvement in regulating the SAC and kinetochore-microtubule attachments is not well characterized.
JOURNAL OF CELL SCIENCE
(2023)
Article
Cell Biology
Xiaoyu Song, Fengrui Yang, Tongtong Yang, Yong Wang, Mingrui Ding, Linge Li, Panpan Xu, Shuaiyu Liu, Ming Dai, Changbiao Chi, Shengqi Xiang, Chao Xu, Dong Li, Zhikai Wang, Lin Li, Donald L. Hill, Chuanhai Fu, Kai Yuan, Pilong Li, Jianye Zang, Zhonghuai Hou, Kai Jiang, Yunyu Shi, Xing Liu, Xuebiao Yao
Summary: EB1 proteins form molecular condensates through liquid-liquid phase separation (LLPS) to constitute the microtubule plus-end machinery, which is crucial for cellular dynamics and organelle movements.
NATURE CELL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Henrietta Onyinye Uzoeto, Samuel Cosmas, Judith Nnedimkpa Ajima, Amarachukwu Vivian Arazu, Chizoba Maryann Didiugwu, Daniel Emmanuel Ekpo, Glory Omini Ibiang, Olanrewaju Ayodeji Durojaye
Summary: This study presents a detailed computational model of the physical interactions within the human CENPHIKM complex, validating the structural similarity with fungal and yeast orthologs. The binding mode of CENP-M to the C-terminus of CENP-I in humans is also confirmed based on existing experimental evidence. Overall, the results support the hypothesis that the human CENP-HIK complex shares similar architecture with its orthologs and highlight the importance of understanding kinetochore functionality and assembly mechanisms.
BENI-SUEF UNIVERSITY JOURNAL OF BASIC AND APPLIED SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Henrietta Onyinye Uzoeto, Judith Nnedimkpa Ajima, Amarachukwu Vivian Arazu, Glory Omini Ibiang, Samuel Cosmas, Olanrewaju Ayodeji Durojaye
JOURNAL OF GENETIC ENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Genetics & Heredity
Glory Omini Ibiang, Joseph Malachi, Mercy Omini Ibiang, Daniel Kenechi Chukwudi, Olanrewaju Ayodeji Durojaye
Summary: The SARS-CoV-2 virus enters the oral mucosa through the ACE2 receptor and undergoes proteolytic cleavage by TMPRSS2 and cathepsin L, allowing it to enter the cellular cytoplasm. Researchers have designed a potential therapeutic peptide to inhibit the activity of TMPRSS2 and highlight the importance of wearing masks for prevention.
EGYPTIAN JOURNAL OF MEDICAL HUMAN GENETICS
(2022)
