Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 324, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2020.128721
Keywords
Fluorescence-based lateral flow immunoassay; Rhombic-like Al nanosupporter; Detection; Tetracycline
Funding
- Fundamental Research Funds from Talented Program [A279021724]
- Northwest AF University [Z111021601]
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Fluorescence-based lateral flow immunoassay (FLFIA) is a prospective technology in detecting tetracycline (TET). However, the existing fluorescent materials (FMs) often require cross-linkers to bind with antibodies (Abs) to form FLFIA FM-Ab probe, which complicates the modification process and may affect the fluorescence intensity of FMs. Herein, the rhombic-like Al nanosupporter (RAlNS) was prepared via a one-step hydrothermal method and used for designing fluorescence nanosupporter for FLFIA to detect TET in real samples. The RAlNS not only possessed excellent water stability, strong fluorescence intensity and favorable thermal stability, but also could adsorb Abs to obtain FMs-Ab probe by simple centrifugation. Under optimal conditions, the limit of detection (LOD) of RAlNS-FLFIA was 0.0516 ng/mL (n = 3), which was 18 times lower than traditional colloidal gold-based LFIA. Meanwhile, the proposed sensor also exhibited high specificity for TET against other antibiotics. Furthermore, the RAlNS-FLFIA was successfully applied to the detection of TET in beef, chicken, milk and honey samples, separately, which demonstrated a significant recovery of 80.40-112.05%. The novel RAlNS-FLFIA provided great potential for the detection of various analytes in food analysis.
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