Article
Chemistry, Analytical
Sixuan Duan, Tianyu Cai, Jia Zhu, Xi Yang, Eng Gee Lim, Kaizhu Huang, Kai Hoettges, Quan Zhang, Hao Fu, Qiang Guo, Xinyu Liu, Zuming Yang, Pengfei Song
Summary: This paper presents a smartphone platform assisted by deep learning for ultra-accurate testing of paper-based microfluidic colorimetric enzyme-linked immunosorbent assay (c-ELISA). The platform eliminates the influences of random lighting conditions and achieves high accuracy in classifying/predicting the concentration of rabbit IgG. A user-friendly smartphone application has been developed to fully automate the sensing process.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Saipriya Ramalingam, Christopher M. Collier, Ashutosh Singh
Summary: Antibiotics are widely used in veterinary science to promote animal health and feed efficiency, but concerns arise due to the potential residues in milk. Therefore, quick and reliable testing methods are essential to ensure the safety of the produced milk.
Article
Nanoscience & Nanotechnology
Tomas Pinheiro, Ana C. Marques, Patricia Carvalho, Rodrigo Martins, Elvira Fortunato
Summary: The article presents a signal transduction method based on gold nanoparticles for the development of paper-based multiplex colorimetric assay devices for diabetes control, showing the potential for multiparametric colorimetric measurement.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biophysics
Antonia Lopreside, Laura Montali, Baojun Wang, Annalisa Tassoni, Maura Ferri, Maria Maddalena Calabretta, Elisa Michelini
Summary: This low-cost biosensor integrates three different biorecognition principles for orthogonal detection of mercury(II) on a three-leaf paper, providing a stable disposable device that can easily be inserted into a smartphone for mercury detection in water samples, with a detection limit reaching ppb levels.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Wenfeng Luo, Jie Deng, Jinhua He, Zeping Han, Chen Huang, Yuguang Li, Qiangqiang Fu, Hanwei Chen
Summary: The novel portable and cost-effective multi-wavelength smartphone colorimetric reader (MW-SCR) designed for liquid colorimetric assays is able to accurately and reliably detect various serum analytes using six LEDs and a smartphone ambient light sensor. The device shows strong linear regression analysis, good repeatability, and can be easily redesigned for multiple applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Brittany AuYoung, Akshay Gutha Ravichandran, Divykumar Patel, Nisarg Dave, Achal Shah, Brianna Wronko-Stevens, Franklin Bettencourt, Reshma Rajan, Nidhi Menon
Summary: This article introduces a device called the KromaHealth Kit, which utilizes image processing and machine learning technology to quantitatively measure total bilirubin in human serum. By depositing biochemical assays on absorbent paper pads and using a dedicated smartphone app to capture images of colorimetric changes, the device can convert them into quantitative values of bilirubin. The research indicates that the KromaHealth Kit can serve as an inexpensive alternative to traditional bilirubin testing in clinical settings, offering high precision, ease-of-use, long shelf-life, and short turnaround time.
FRONTIERS IN CHEMISTRY
(2022)
Article
Food Science & Technology
Xuemei Tang, Ruiqi Su, Haixi Luo, Ying Zhao, Lu Feng, Jian Chen
Summary: The study introduced a method for rapid detection of Aflatoxin B-1 (AFB(1)) using microfluidic paper-based analytical devices (mu PADs), which exhibited superior detection performance in actual food samples and met the testing requirements of countries like China.
Article
Chemistry, Multidisciplinary
Sarida Naorungroj, Prinjaporn Teengam, Tirayut Vilaivan, Orawon Chailapakul
Summary: A smartphone-assisted paper-based colorimetric assay was developed for early screening of HPV DNA, offering a simple, sensitive, and selective way of detection. The method quantifies DNA concentration by analyzing color changes, allowing for linear measurement in the range of 1 to 1000 nM.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Chemistry, Applied
Sufian Rasheed, Muhammad Anwar ul Haq, Naseer Ahmad, Dilshad Sirajuddin, Dilshad Hussain
Summary: This work presents a new colorimetric sensor that can rapidly, sensitively, and selectively detect permethrin using metronidazole-stabilized silver nanoparticles (MTZ-AgNPs). The sensor has a limit of detection (LOD) and a limit of quantification (LOQ) of 0.0104 μM and 0.0348 μM, respectively. It has been successfully integrated with smartphone and microfluidic fabrication of paper-based analytical devices (μPADs) for real-time and on-site detection of permethrin.
Article
Environmental Sciences
P. Rajasulochana, Yaswanth Ganesan, P. Senthil Kumar, S. Mahalaxmi, Fahira Tasneem, Muthamilselvi Ponnuchamy, Ashish Kapoor
Summary: To ensure safe drinking water, a paper-based microfluidic sensor was developed for quantitative estimation of nitrite in water. The sensor, fabricated using a simple punching machine and assisted by smartphone image analysis, showed linear detection of nitrite in the range of 0.1 to 10 ppm.
ENVIRONMENTAL RESEARCH
(2022)
Article
Chemistry, Analytical
Xiaoqing Lv, Zhengtai Ma, Jiaqi Xu, Zhen Wang, Guixian Zhu, Beiju Huang
Summary: This paper reports a simple and innovative approach using parylene C to fabricate paper-based chips and construct a point-of-care testing platform through smartphones. The method is low-cost, fast, and highly sensitive, showing potential application in biochemical detection.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Xu Zhu, Jing Tang, Xilian Ouyang, Yibo Liao, Haopeng Feng, Jiangfang Yu, Li Chen, Yating Lu, Yuyang Yi, Lin Tang
Summary: In this study, a portable paper-based colorimetric sensor with a smartphone platform was developed for on-site and quantitative analysis of antibiotic residues. The method is based on aptamer-regulated nanozyme activity and shows an ultrahigh sensitivity. This analysis platform has excellent potential for on-site and mass screening of antibiotic residues.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Lorena Placer, Isela Lavilla, Francisco Pena-Pereira, Carlos Bendicho
Summary: This study presents the development of a 3D origami microfluidic paper-based analytical device (3D mu PAD) for the determination of iodide and iodate in edible seaweeds by colorimetric detection using a smartphone. The addition of a methacrylate holder improved the interlayer contact in 3D mu PADs, resulting in enhanced sensitivity and precision, as well as real-time monitoring. The assay relies on the formation of a blue triiodide-starch complex at the detection areas of the 3D mu PAD, providing an affordable, fast, and environmentally friendly method for simultaneous determination of inorganic iodine species. Under optimal conditions, the method achieved low limits of detection and quantification for iodide and iodate.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Wei-Yi Zhang, Tao Tian, Li-Jing Peng, Hang-Yu Zhou, Hao Zhang, Hua Chen, Feng-Qing Yang
Summary: In this study, a paper-based analytical device combined with a smartphone was developed for the dual-mode fluorescent and colorimetric detection of beta-glucosidase activity. The device utilizes the hydrolysis of a substrate to generate fluorescent and colorimetric signals, which are then captured and analyzed using a smartphone. This method offers a simple, environmentally friendly, and cost-effective approach for beta-glucosidase activity detection.
Article
Spectroscopy
Marilia Christian Gomes Morais Nascimento, Maria Carolina Robaina Vieira, Fabio R. P. Rocha, Tiago Almeida Silva, Willian Toito Suarez
Summary: This study developed and validated two procedures based on DPD and OT for the determination of residual free chlorine in water using a flow-based microdevice built with LTCC technology. The analytical signal was monitored by a smartphone camera using RGB values obtained by a free application. The optimized conditions resulted in linear ranges, low limits of detection, and good precision for both DPD and OT methods. Both procedures were successfully applied to water treatment plant samples, demonstrating the innovative, sustainable, and cost-effective nature of the flow-based microdevice coupled to digital-image colorimetry.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Lucas C. Duarte, Igor Pereira, Lanaia I. L. Maciel, Boniek G. Vaz, Wendell K. T. Coltro
Summary: The article discusses the development of a microfluidic mixing device fabricated entirely by 3D printing technology to monitor chemical reactions via mass spectrometry. Results showed that the device containing 3D serpentine channels had higher relative abundance values, making it a promising tool for monitoring organic reactions.
ANALYTICA CHIMICA ACTA
(2022)
Article
Food Science & Technology
Kariolanda C. A. Rezende, Lucas M. Duarte, Kemilly M. P. Pinheiro, Thiago M. G. Cardoso, Sandro A. Nogueira, Wendell K. T. Coltro
Summary: This report discusses the use of portable analytical platforms combined with chemometrics to trace the authenticity of whisky. The rapid analysis methods based on separation and colorimetric reactions as well as pH and conductivity measurements were successfully applied to distinguish adulterated and authentic whisky samples.
FOOD ANALYTICAL METHODS
(2022)
Article
Chemistry, Analytical
Lucas F. Castro, Habdias A. Silva-Neto, Lucas R. Sousa, William R. de Araujo, Wendell K. T. Coltro
Summary: This study describes the development of electrochemical paper-based analytical devices (ePADs) using carbon-based paste combining silicone glue and graphite powder. The ePADs were manufactured using the screen-printing technique and an alternative electrical connector. The proposed ePADs showed high repeatability, reproducibility, and stable responses, and had linear response for ascorbic acid (AA) in the range of 0.1 to 2.0 mmol L-1, with a limit of detection of 4.0 mu mol L-1. The ePADs were evaluated for AA analysis in synthetic biofluids, vitamin C tablets, and food samples, and exhibited similar or even superior performance compared to other reports in the literature, showing its feasibility for sensing applications.
Review
Chemistry, Analytical
Habdias A. Silva-Neto, Iana V. S. Arantes, Andre L. Ferreira, Guida H. M. do Nascimento, Gabriel N. Meloni, William R. de Araujo, Thiago R. L. C. Paixa, Wendell K. T. Coltro
Summary: Paper-based microfluidic devices have become powerful platforms for bioanalysis due to their simpler fabrication protocols, compatibility with multi-purpose detectors, and global affordability. This review provides an overview of manufacturing methods, integrative sensing materials, the clinical relevance of biomarkers and bioanalysis, as well as examples of applications involving various samples and diseases. It also discusses the recent developments in paper-based wearable devices and hybrid sensors for clinical applications.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Habdias A. Silva-Neto, Gerson F. Duarte-Junior, Danielly S. Rocha, Fethi Bedioui, Anne Varenne, Wendell K. T. Coltro
Summary: In this study, a recyclable approach using ABS residues and graphite flakes was proposed to create a conductive paste. The mixture showed greater adherence to different substrates and was used to construct a paper-based electrochemical sensor. The electrochemical properties of the recycled electrode material were similar to those of traditional carbon-based surfaces, and the proposed sensor exhibited promising performance in nitrite detection.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Danielly S. Rocha, Richard P. S. de Campos, Habdias A. Silva-Neto, Gerson F. Duarte-Junior, Fethi Bedioui, Wendell K. T. Coltro
Summary: Digital microfluidics (DMF) is a versatile lab-on-a-chip platform integrated with UV-LEDs for sample degradation and colorimetric detection, as demonstrated in the analysis of S-nitrosocysteine (CySNO). The proposed integration showed satisfactory correlation with the results from a desktop scanner, and the analytical parameters revealed linear behavior in the CySNO concentration range of 12.5-400 mu mol L-1, with a limit of detection of 2.8 mu mol L-1. Synthetic serum and human plasma samples were successfully analyzed with no statistical difference observed.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Lucas R. Sousa, Habdias A. Silva-Neto, Lucas F. Castro, Karoliny A. Oliveira, Federico Figueredo, Eduardo Corton, Wendell K. T. Coltro
Summary: This paper presents the design and construction of dual microfluidic paper-based analytical devices (dual-mu PADs) as a do-it-yourself lab-on-paper platform. The devices utilize both colorimetric and electrochemical modules to achieve signal readout sensing. The fabrication process involves using a 3D pen polymeric resin to create graphite carbon-based electrodes and hydrophobic barriers on paper substrates. The performance of the dual-mu PADs was evaluated for the analysis of lactate, pH, nitrite, and salivary amylase (sAA), with successful testing on saliva samples from healthy individuals and those with periodontitis. The devices show potential for point-of-care diagnosis of periodontal disease by sensitive detection of biomarkers in saliva.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Vanderli Garcia Leal, Habdias A. Silva-Neto, Sidnei Goncalves da Silva, Wendell Karlos Tomazelli Coltro, Joao Flavio da Silveira Petruci
Summary: This study presents a quadcopter drone equipped with a fully integrated 3D-printed analytical laboratory for monitoring H2S concentration in the atmosphere. The analytical method used was based on the fluorescence quenching reaction between fluorescein mercuric acetate and H2S. The proposed device allows detection of H2S in the range of 15-200 ppbv.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Kemilly M. P. Pinheiro, Alysson V. F. Sako, Marcella F. Rodrigues, Boniek G. Vaz, Iris Medeiros Junior, Rogerio M. Carvalho, Wendell K. T. Coltro
Summary: A capillary electrophoresis-mass spectrometry method was developed for the analysis of naphthenic acids in produced water samples. The method showed good repeatability and linear behavior within certain concentration ranges for different acids. It successfully identified and quantified the targeted acids in real water samples, demonstrating its effectiveness in complex sample analysis.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Laisa C. Oliveira, Danielly S. Rocha, Habdias A. Silva-Neto, Thaisa A. C. Silva, Wendell K. T. Coltro
Summary: This study describes the development of a disposable electrochemical paper-based analytical device (ePAD) using a new formulation of conductive ink. The ePAD showed good analytical performance for paracetamol sensing, with linear behavior in the concentration range of 1-60 μmol L-1, a limit of detection of 0.2 μmol L-1, and satisfactory reproducibility. The device's environmental impact was assessed and found to be eco-friendly.
Article
Chemistry, Analytical
Lorrayne G. A. Dias, Lucas C. Duarte, Kemilly M. P. Pinheiro, Nikaele S. Moreira, Wendell K. T. Coltro
Summary: The goal of green chemistry is to reduce the environmental impact of chemical processes. This study developed an environmentally friendly methodology using paper-based analytical devices (μPADs) for acid-base titrations. The method was successfully demonstrated for different types of solutions, and the proposed approach was evaluated based on the 12 principles of green analytical chemistry.
Article
Chemistry, Multidisciplinary
Habdias A. Silva-Neto, Gerson F. Duarte-Junior, Gabriela Silverio Bazilio, Juliana Alves Parente-Rocha, Diego B. Colugnati, Clayton L. Borges, Wendell K. T. Coltro
Summary: This report presents the fabrication of a low-cost magnetic separator holder using 3D printing and neodymium blocks for extracting and purifying RNA from samples collected from patients infected by SARS-CoV-2. The device successfully demonstrated the proof of concept through the extraction and purification of swab samples collected from eight suspected patients. The performance of the printed device was comparable to a commercial magnetic separator, and the use of 3D printing and neodymium blocks showed potential for COVID-19 diagnosis.
Article
Chemistry, Analytical
Nikaele S. Moreira, Kemilly M. P. Pinheiro, Lucas R. Sousa, Gabriel D. S. Garcia, Federico Figueredo, Wendell K. T. Coltro
Summary: Whisky adulteration is a prevalent practice driven by high costs. This study proposes a paper-based microfluidic analytical device for rapid and cost-effective detection of paracetamol (PAR) in whisky. The method showed excellent sensitivity and can be considered a viable and portable alternative for analyzing beverages at criminal scenes, customs, and police operations.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Danielly Santos Rocha, Habdias Araujo Silva-Neto, Laisa Cristina de Oliveira, Shellyda Goncalves Guimaraes Souza, Mario Henrique Palis Santana, Wendell Karlos Tomazelli Coltro
Summary: This study demonstrates the use of stencil-printed carbon electrodes and square-wave voltammetry (SWV) as a powerful and robust analytical tool for forensic investigations of the concentration of sildenafil citrate (SC) in commercial and adulterated tablets.
BRAZILIAN JOURNAL OF ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)