Article
Chemistry, Applied
Prithvi Pal Singh, Patil Shivprasad Suresh, Prateek Singh Bora, Vinod Bhatt, Upendra Sharma
Summary: This study reported the isolation of nine steroidal saponins from the rhizomes of Trillium govanianum, including one new compound. The plant is traditionally used for treating various inflammatory, sexual disorders, infections, and wound healing, and its active components are believed to be steroidal saponins.
NATURAL PRODUCT RESEARCH
(2022)
Article
Biochemical Research Methods
Bowen Huang, Adabelle Ong, Wenli Wang, Yu He, Yongjun Xiao
Summary: The detection and understanding of emerging contaminants (ECs) pose new challenges to water pollution control. Triclosan (TCS), an emerging contaminant widely found in the environment, is a commonly used antibacterial agent. Microdialysis (MD), a sampling technique, combined with UHPLC-QTOF/MS analysis, was used to identify endogenous metabolites as biomarkers of TCS exposure in living crucian carp. The quantification of these biomarkers using UHPLC-MS/MS provided continuous monitoring of the effect of TCS on endogenous metabolites, contributing to a better understanding of its toxicological effect.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Xu Pang, Ling-fei Wan, Jie Yang, Pei-ying Bai, Jie Zhang, Xiao-juan Chen, Xin-long Yan, Bai-ping Ma
Summary: A study has shown that steroidal glycosides derived from Trillium tschonoskii rhizomes have potential implications in the therapy of human intrahepatic cholangiocarcinoma (ICC) by significantly repressing tumor colony and sphere formation, as well as inhibiting cancer stem markers.
BIOORGANIC CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Misbah Tabassum, Bashir Ahmad Lone, Mudasir Nazir Bhat, Anil Bhushan, Nagma Banjare, Esteban Manrique, Prasoon Gupta, Dilip M. Mondhe, Prem N. Gupta
Summary: The study investigated the potential of Trilliumoside A, a natural product-derived molecule, as an effective anticancer agent. The compound showed significant cytotoxic activity against human lung cancer cells, induced apoptosis by regulating apoptotic proteins, and arrested the cell cycle. The findings suggest that Trilliumoside A has potential as a promising anticancer drug.
Article
Engineering, Chemical
Patil Shivprasad Suresh, Prithvi Pal Singh, Smita Kapoor, Yogendra S. Padwad, Upendra Sharma, Upendra Anmol
Summary: Natural deep eutectic solvents, such as lactic acid-derived NADESs, have shown high solvation and food-grade characteristics, making them a preferred choice for extracting bioactive compounds. This study focused on investigating the effects of different NADESs on the extraction of steroidal saponins from Trillium govanianum Wall. ex D.Don. It was found that choline chloride: lactic acid (1:1) was the most effective eutectic solvent for the extraction, resulting in higher extractability of steroidal saponins compared to hydro-ethanolic solvent-based extraction. The optimized parameters were validated, and the cytocompatibility of NADESs with animal cells was also evaluated.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Yiming Zhang, Lanlan Zhan, Quan Wen, Yulin Feng, Yun Luo, Ting Tan
Summary: This study discovered that taxifolin is able to effectively trap methylglyoxal (MGO), an important precursor of advanced glycation end products (AGEs), by forming MGO adducts in vitro and in vivo. Quantification studies showed that taxifolin and its metabolites can dose-dependently trap MGO. Similar results were observed in diabetic mice.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Analytical
Ge Xun, Yuhuan Tian, Yanhua Gao, Jia Zhang, Xin Liu, Shuo Sun, Qi Qian, Fangfang Liu, Qiao Wang, Xu Wang
Summary: This study identified and compared chemical compounds in three types of commercial Tripterygium wilfordii genus preparations (TWGP) using UHPLC-QTOF-MS/MS and molecular networking (MN) technology. A total of 103 compounds were identified, with 26 of them being discovered as main differential compounds in Tripterygium glycoside tablets. This study lays the foundation for classifying effective and toxic substances and TWGP quality control, utilizing MN technology for compound analysis in TWGP.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Joanna Wittckind Manoel, Gabriele Bordignon Primieri, Livia Maronesi Bueno, Camila F. A. Giordani, Julia Medeiros Sorrentino, Alexsandro Dallegrave, Clesio Soldateli Paim, Elfrides Eva Scherman Schapoval, Cassia Virginia Garcia, Martin Steppe
Summary: A simple and fast analytical method was developed and validated to determine EMPA and its synthetic impurities using UHPLC-QTOF-MS, with parameters such as specificity, linearity, precision, and accuracy within acceptable ranges. Additionally, two new degradation products were observed under exposure conditions, contributing to the understanding of the EMPA impurity profile.
MICROCHEMICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
So-Jeong Lee, Heon-Woong Kim, Suji Lee, Ryeong Ha Kwon, Hyemin Na, Ju Hyung Kim, Chi-Do Wee, Seon Mi Yoo, Sang Hoon Lee
Summary: This report provides chromatographic and mass fragment ion data of 38 identified saponins in Platycodon grandiflorum (PG), including new compounds. Results showed that the roots of PG have the highest total content of saponins, with the platycodigenin group being the major component. Additionally, blanching of PG satisfied the optimal temperature condition for enzyme activation.
Article
Pharmacology & Pharmacy
Patil Shivprasad Suresh, Prithvi Pal Singh, Yogendra S. Padwad, Upendra Sharma
Summary: The study demonstrated that extracts, fractions, and isolated steroidal saponins from Trillium govanianum exhibit good inhibitory activity against alpha-amylase and alpha-glucosidase, with moderate activity against dipeptidyl peptidase IV. In silico and in vitro studies revealed an association between steroidal saponins from T. govanianum and their molecular interactions with these enzymes. These findings suggest that fractions and steroidal saponins from T. govanianum could be used as nutraceutical supplements for managing blood glucose levels effectively.
JOURNAL OF PHARMACY AND PHARMACOLOGY
(2021)
Article
Agronomy
Alexandra G. Duran, Javier Benito, Francisco A. Macias, Ana M. Simonet
Summary: Agave saponins have been identified as a valuable resource for the development of new agrochemicals. By optimizing extraction methods and testing various saponin fractions, researchers found that certain fractions from Agave species within the subgenus Agave showed high levels of activity when tested on wheat etiolated coleoptiles. Utilizing advanced techniques like UPLC-MS and NMR spectroscopy, the study identified 20 aglycones in the saponin fractions without the need for isolation or hydrolysis. Additionally, the study highlighted the potential use of these saponins for developing natural herbicides, showing promising results against weeds.
Article
Chemistry, Applied
Allan dos Santos Polidoro, Claissa Nied Peixoto, Vitoria Carolina Prestes de Souza, Anai Loreiro dos Santos, Adriano de Araujo Gomes, Rosangela Assis Jacques
Summary: This study aimed to develop strategies to elucidate the phenolic composition of yerba mate samples from Brazil, Argentina, Uruguay, and Paraguay. The results showed that Brazilian samples had higher pigment content, while samples from other countries had higher phenolic content.
Article
Plant Sciences
Xiaoli Wang, Qiaoyu He, Qian Chen, Beibei Xue, Jia Wang, Tao Wang, Hong Liu, Xiaopeng Chen
Summary: This study combined network pharmacology and metabolomics to explore the potential mechanism of Shenyan Kangfu Tablets (SYKFT) in treating diabetic nephropathy (DN), identifying potential active components, related targets, and biomarkers. The results suggest that the potential mechanism of SYKFT in treating DN is most related to insulin resistance (IR), demonstrating the feasibility of using network pharmacology and metabolomics to study the mechanisms of herbal treatments for complex diseases.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Chemistry, Applied
Diletta Piatti, Simone Angeloni, Filippo Maggi, Giovanni Caprioli, Massimo Ricciutelli, Lolita Arnoldi, Stefania Bosisio, Giacomo Mombelli, Ettore Drenaggi, Gianni Sagratini
Summary: The polar extracts and essential oils of sea fennel cultivated in central Italy were analyzed. The extracts contained 21 bioactive compounds, with 5-O-caffeoylquinic acid being the most abundant. Melatonin, a hormone that regulates sleep/wake cycle, was identified for the first time in sea fennel extract. The essential oil was mainly composed of monoterpene hydrocarbons and oxygenated monoterpenes. This study highlights the potential use of sea fennel as an edible plant and a source of nutraceutical compounds.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Agriculture, Dairy & Animal Science
Linli Zhang, Li Li, Qingwu Xin, Zhiming Zhu, Zhongwei Miao, Nenzhu Zheng
Summary: The chemical composition of breast meats of Liancheng white duck and Cherry Valley duck were examined, revealing significant differences in meat quality between the two breeds. Liancheng white duck had higher shear force and pH values, but lower cooking loss, lightness, redness, and yellowness compared to Cherry Valley duck. Metabolomic analysis identified significant differences in metabolites between the two duck breeds, with Liancheng white duck exhibiting higher nutritional and medicinal value.
Article
Energy & Fuels
Shivani Puri, Sarthak Sharma, Avnesh Kumari, Mohit Sharma, Upendra Sharma, Sanjay Kumar
Summary: The continuous growth of livestock population has led to the generation of significant amounts of waste. Inadequate management and disposal of animal waste pose serious public health and environmental issues. Recent research has shown that using lignocellulosic biomass for producing biofuels and biotextiles is a promising alternative for efficient utilization of animal waste biomass.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Biochemistry & Molecular Biology
Patil Shivprasad Suresh, Krishan Gopal Thakur, Upendra Sharma
Summary: In this study, we conducted in silico screening of steroidal sapogenins (SS) from the genus Trillium to identify potential agonists for the glucocorticoid receptor (GR). Our findings suggest that two SS molecules (SS 38 and SS 39) exhibit favorable binding to GR, as revealed by docking scores and MM-GBSA analysis. Molecular dynamics simulation studies further confirmed stable interactions between these SS molecules and GR. These findings indicate that SS 38 and SS 39 may have potential as agonists for GR-mediated diseases.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Food Science & Technology
Prithvi Pal Singh, Robin Joshi, Ravi Kumar, Ashok Kumar, Upendra Sharma
Summary: This study conducted a detailed phytochemical analysis of F. assa-foetida in India and compared it with F. jaeschkeana and commercial samples of F. assa-foetida oleo-gum resin. Various compounds were identified and 141 metabolites were identified using the METLIN database. The nutritional analysis revealed major amino acids among the samples. Sulfurous compounds were identified in all samples except F. jaeschkeana. The study also showed significant differences in volatile constituents and revealed associations between biosynthetic pathways in these two species. This study explains why only F. assafoetida is used in culinary applications instead of F. jaeschkeana.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Green & Sustainable Science & Technology
Patil Shivprasad Suresh, Prithvi Pal Singh, Mohit Sharma, Upendra Sharma
Summary: Using natural deep eutectic solvents (NADESs) as greener substitutes for organic solvents, the current study investigated the extraction of steviol glycosides from Stevia rebaudiana. The results showed that NADES 50 (lactic acid: glycerol: malic acid: glucose, 1:1:1:1) was the most efficient in extracting rebaudioside A and stevioside, while NADES 36 (choline chloride: lactic acid: oxalic acid: glycerol, 2:2:1:1) extracted all targeted analytes along with untargeted compounds. The study concluded that multicomponent NADESs have excellent extractability of steviol glycosides compared to binary NADESs.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Chemistry, Organic
Ankita Thakur, Shiv Shankar Gupta, Ankit Kumar Dhiman, Upendra Sharma
Summary: A photocatalytic approach has been used to introduce a trifluoroethanol unit onto isoquinolines by employing N-trifluoroethoxyphthalimide as a hydroxyfluoroalkyl radical precursor. The involvement of a free-radical pathway was confirmed by radical quenching experiments, and DFT calculations supported the intramolecular 1,2-HAT reactivity of the trifluoroethoxy radical with the trifluoroethanol radical. Fluorescence quenching studies revealed that isoquinoline was responsible for the quenching of Ir photocatalyst emission. A catalytic cycle involving the reaction of trifluoroethanol radical with isoquinolines was proposed.
JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Agronomy
Sandeep Kumar, Anmol, Upendra Sharma, S. G. Eswara Reddy
Summary: In the current study, the residual toxicity of extracts, fractions, and isolated compounds of Aconitum heterophyllum were evaluated against Aphis craccivora. The ethanolic and aqueous methanolic extracts showed effective control against A. craccivora. Among the fractions, the n-butanol fraction of the aqueous methanolic extract was the most effective, followed by the ethyl acetate fraction of the ethanolic extract and the n-hexane fraction of both extracts. Aconitic acid was identified as the most effective pure molecule. The study also suggested that the action of the ethanoic extract might involve the inhibition of glutathione S-transferase in A. craccivora.
PEST MANAGEMENT SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Shiv Shankar Gupta, Shivangi Gupta, Puneet Manisha, Puneet Guptag, Upendra Sharma
Summary: Selective C7-allylation of indolines with allyl bromide under ruthenium catalysis is demonstrated in this study. Good selectivity and yields were achieved for the C7-allylation of various indolines, including drug compounds, under established reaction conditions. Combined experimental and density functional theory (DFT) studies suggest that olefin insertion is the energetically favorable pathway among four possible routes. Furthermore, experimental and DFT studies reveal that C-H activation is a reversible rate-limiting step.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Prithvi Pal Singh, Patil Shivprasad Suresh, Upendra Anmol, Upendra Sharma
Summary: Four new compounds were isolated from the rhizomes of Trillium govanianum, including some rare sugar moieties. This is also the first report on the isolation of certain compounds from the Trillium genus, as well as feruloyl sucrose derivatives and (E)-4-hydroxy-dodec-2-enedioic acid. One of the compounds showed inhibitory activity against acetylcholinesterase.
Article
Chemistry, Multidisciplinary
Prakhar Agrawal, Surekha Kumari, Asif Mohmmed, Pawan Malhotra, Upendra Sharma, Dinkar Sahal
Summary: This study identified eight compounds with promising binding affinity and antiparasitic efficacy against Plasmodium parasites. Two of these compounds showed significant suppression of parasitemia in a mouse model of malaria.
Article
Chemistry, Multidisciplinary
Sumit, Devesh Chandra, Upendra Sharma
Summary: Chiral phosphoric acids have emerged as leading ligands in enantioselective C-H activation, offering high enantiomeric excess and excellent control over regio and chemo-selectivity. This review summarizes their use in this exciting field, highlighting the induction of chirality through different interactions with the substrate.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Mehak Sharma, Patil Shivprasad Suresh, Shiv Shankar Gupta, Upendra Sharma
Summary: In this study, natural deep eutectic solvents (NADESs) were used to extract bioactive compounds from the Himalayan medicinal herb Aconitum heterophyllum Wall. The most effective NADES-based solvent system was found to be NADES-15 [lactic acid: glycerol (1:1)], which showed higher extraction efficiency compared to conventional solvents. The efficiency of NADES-15 extraction was further improved by optimizing parameters such as solid: liquid ratio, extraction time, temperature, and water content. The recovered NADES-15 showed relatively lower extraction efficiency than the fresh counterpart, and molecular mechanism studies suggested that higher exergonic stability and intermolecular hydrogen bond interactions might contribute to the enhanced extraction efficiency of NADESs compared to conventional solvents.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2023)
Article
Food Science & Technology
Gaurav Anmol, Gaurav Aggarwal, Upendra Sharma
Summary: An ultra-high performance liquid chromatography coupled to a diode array detector (UHPLC-DAD) based method was developed for the simultaneous determination of 21 bioactive phenolic compounds in food products. The method was validated and showed ideal results, and was successfully applied for the quantitative analysis of phenolics in different targeted food samples.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Chemistry, Physical
Devesh Chandra, Nikunj Kumar, Puneet Sumit, Puneet Gupta, Upendra Sharma
Summary: A comparative study of C8-alkylation of isoquinolone with maleimides was conducted, and Ru and Rh catalysts were found to be excellent in providing desired products. Density functional theory (DFT) investigations and non-covalent interaction (NCI) analysis revealed the reaction mechanism and the selective formation of alkylated products. This catalytic methodology also showed a broad substrate scope and late-stage modification.
MOLECULAR CATALYSIS
(2023)
Article
Plant Sciences
Patil Shivprasad Suresh, Veerbhan Kesarwani, Surekha Kumari, Ravi Shankar, Upendra Sharma
Summary: The diverse chemical scaffolds of Cissampelos pareira have pleiotropic therapeutical applications. The flavonoids in C. pareira have potential as antimalarial agents and showed good binding affinity with selected Plasmodium proteins. Molecular docking and molecular dynamics simulation revealed stable complexes between flavonoids and antimalarial targets. These findings suggest that C. pareira and its flavonoids could be developed as broad-spectrum antimalarial therapeutics.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Review
Biochemistry & Molecular Biology
Shivprasad Suresh Patil, Rohit Sharma, Upendra Sharma, Yogendra Padwad
Summary: Emerging evidence suggests that sustained diabetes-associated factors contribute to abnormal cell proliferation and neoplastic transformation. Epidemiological studies have also shown a link between diabetes and cancer, often associated with a sedentary lifestyle. However, our understanding of the molecular mechanisms connecting hyperglycemia and oncogenic transformation is limited. A recent study discovered that hyperglycemia inactivates AMPK, destabilizes TET2, and increases the risk of cancer in patients with diabetes. Utilizing natural products derived from plants may offer a pharmacological approach to managing hyperglycemia associated with cancer.
CHEMICAL BIOLOGY LETTERS
(2023)