Article
Cell Biology
Xuejing Ren, Hang Zhao, Lin Shi, Zhengran Li, Ruiyan Kong, Rui Ma, Lemei Jia, Shan Lu, Jian-Hua Wang, Meng-Qiu Dong, Yingchun Wang, Zhouhua Li
Summary: Stem cell proliferation is regulated by phosphorylation, and the interdependence of phosphorylation sites affects the function of Yun in intestinal stem cell proliferation.
CELL PROLIFERATION
(2022)
Article
Genetics & Heredity
Arafath K. Najumudeen, Fatih Ceteci, Sigrid K. Fey, Gregory Hamm, Rory T. Steven, Holly Hall, Chelsea J. Nikula, Alex Dexter, Teresa Murta, Alan M. Race, David Sumpton, Nikola Vlahov, David M. Gay, John R. P. Knight, Rene Jackstadt, Joshua D. G. Leach, Rachel A. Ridgway, Emma R. Johnson, Colin Nixon, Ann Hedley, Kathryn Gilroy, William Clark, Sudhir B. Malla, Philip D. Dunne, Giovanny Rodriguez-Blanco, Susan E. Critchlow, Agata Mrowinska, Gaurav Malviya, Dmitry Solovyev, Gavin Brown, David Y. Lewis, Gillian M. Mackay, Douglas Strathdee, Saverio Tardito, Eyal Gottlieb, Zoltan Takats, Simon T. Barry, Richard J. A. Goodwin, Josephine Bunch, Martin Bushell, Andrew D. Campbell, Owen J. Sansom
Summary: Colorectal tumors with mutated KRAS and APC rely on the amino acid transporter SLC7A5 for tumorigenesis and enhanced protein synthesis. Targeting SLC7A5 could be an attractive approach for therapy-resistant KRAS-mutant colorectal cancer, as it plays a critical role in maintaining intracellular amino acid levels and supporting proliferation.
Article
Cell & Tissue Engineering
Zhidong Zhao, Yuxing Wang, Qian Wang, Jiawu Liang, Wei Hu, Sen Zhao, Peilin Li, Heng Zhu, Zhongli Li
Summary: The study found that r-ESW can promote the self-renewal of SCB-SPCs in vitro by targeting YAP activity and enhance its repair efficiency in vivo, indicating promising application prospects.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Genetics & Heredity
Elisa Baldelli, Emna El Gazzah, John Conor Moran, Kimberley A. Hodge, Zarko Manojlovic, Rania Bassiouni, John D. Carpten, Vienna Ludovini, Sara Baglivo, Lucio Crino, Fortunato Bianconi, Ting Dong, Jeremy Loffredo, Emanuel F. Petricoin, Mariaelena Pierobon
Summary: KRAS mutations are common oncogenic drivers in NSCLC and lung adenocarcinomas, targeting which has been challenging leading to indirect inhibition of downstream targets. Exploring the impact of wild-type KRAS on druggable targets can uncover new vulnerabilities, with FoxM1 emerging as a potential vulnerability in KRAS mutant lung adenocarcinomas.
Article
Cell Biology
Chen-Yuan Tseng, Michael Burel, Michael Cammer, Sneh Harsh, Maria Sol Flaherty, Stefan Baumgartner, Erika A. Bach
Summary: This study reveals that germline stem cells lacking the transcription factor Chinmo have a competitive advantage and can replace the entire germline stem cell pool over time. The study also suggests that the influence of germline stem cell competition may extend beyond individual stem cell niche dynamics to population-level allelic drift and evolution.
DEVELOPMENTAL CELL
(2022)
Article
Oncology
Daqi Jia, Leilei Li, Peng Wang, Qiang Feng, Xinyan Pan, Peng Lin, Shuling Song, Lilin Yang, Julun Yang
Summary: The study discovered that ZNF24 promotes the growth of KRAS mutant lung adenocarcinoma by upregulating SLC7A5 protein expression, suggesting that ZNF24 may serve as a new biomarker and therapeutic target for KRAS mutant tumors.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
You Ji Kim, Kwang Hwan Park, Kyoung-Mi Lee, Yong-Min Chun, Jin Woo Lee
Summary: Ubiquitin-specific protease 7 (USP7) plays a crucial role in regulating self-renewal and differentiation of hBMSCs, especially in the early stages of osteogenic, adipogenic, and chondrogenic differentiation. USP7 acts as an upstream regulator of SOX2 and NANOG, and its deficiency leads to downregulation of these self-renewal regulating proteins. Targeting USP7 could be a potential strategy to preserve the self-renewal capacity of hBMSCs for stem cell therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
J. Nathaniel Diehl, Jennifer E. Klomp, Kayla R. Snare, Priya S. Hibshman, Devon R. Blake, Zane D. Kaiser, Thomas S. K. Gilbert, Elisa Baldelli, Mariaelena Pierobon, Bjorn Papke, Runying Yang, Richard G. Hodge, Naim U. Rashid, Emanuel F. Petricoin, Laura E. Herring, Lee M. Graves, Adrienne D. Cox, Channing J. Der
Summary: The study demonstrates that depletion of KRAS in KRAS-mutant pancreatic cancer leads to changes in a panel of kinases, with upregulation of certain kinases promoting cancer growth and downregulation of others playing a key role in KRAS-driven proliferation. Inhibition of upregulated kinases like DDR1 and WEE1 can effectively suppress PDAC growth. Additionally, concurrent inhibition of WEE1 and ERK results in potent growth suppression and enhanced apoptotic death compared to WEE1 inhibition alone.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Cell Biology
Mukesh. K. K. Sriwastva, Yun Teng, Jingyao Mu, Fangyi Xu, Anil Kumar, Kumaran Sundaram, Rajiv Kumar Malhotra, Qingbo Xu, Joshua. L. L. Hood, Lifeng Zhang, Jun Yan, Michael. L. L. Merchant, Juw Won Park, Gerald. W. W. Dryden, Nejat. K. K. Egilmez, Huang-Ge Zhang
Summary: This study demonstrates that a protein in extracellular vesicles (EVs) acts as an organizer of protein networks, promoting lung inflammation and tumor growth through the Fn1/IL-17A/FGF21 pathway. EV-G12D-mutant KRAS was shown to interact with extracellular vesicular factors via fibronectin-1 (Fn1), activating the IL-17A/FGF21 inflammation pathway in recipient cells. These findings provide a new understanding of EV function with potential implications for therapeutic interventions in EV-mediated diseases.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Review
Cell Biology
Feihong Deng, Zengrong Wu, Fei Zou, Su Wang, Xuehong Wang
Summary: This article provides an overview of the Hippo-YAP/TAZ signaling pathway and the processes of intestinal self-renewal and regeneration. The roles of YAP/TAZ in different phases are summarized, suggesting a potential strategy for treating inflammatory bowel disease (IBD).
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Oncology
Miaoling Tang, Meisongzhu Yang, Geyan Wu, Shuang Mo, Xingui Wu, Shuxia Zhang, Ruyuan Yu, Yameng Hu, Yingru Xu, Ziwen Li, Xinyi Liao, Jun Li, Libing Song
Summary: In liver cancer cells, the upregulation of MFF mediated by the TBX19/PRMT1 complex promotes mitochondrial fission and tumor-initiating capacity, indicating that PRMT1 may be a viable therapeutic target in liver cancer.
Article
Medicine, Research & Experimental
Dianyuan Zhao, Fengjiao Yang, Yang Wang, Site Li, Yang Li, Fei Hou, Wenting Yang, Di Liu, Yuandong Tao, Qian Li, Jing Wang, Fuchu He, Li Tang
Summary: Tissue-derived signals regulate the identity and function of Kupffer cells through the BMP9/BMP10/ALK1 signaling pathway, while ALK1 is dispensable for the maintenance of macrophages.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Materials Science, Multidisciplinary
Yan Hu, Guoyou Huang, Jin Tian, Jinbin Qiu, Yuanbo Jia, Dayun Feng, Zhao Wei, Sidi Li, Feng Xu
Summary: In this study, Xu et al. investigated the responses of astrocytes to changes in matrix stiffness using an in vitro model. They found that astrocytes exhibit different phenotypes in hydrogels of varying stiffness, with the ability to switch between phenotypes in situ. Additionally, the study highlights the role of matrix stiffness in astrocytic activation and astrogliosis, mediated by Yes-associated protein (YAP) signaling, suggesting potential therapeutic implications for regulating glial scar formation.
NPG ASIA MATERIALS
(2021)
Article
Multidisciplinary Sciences
Takeshi Fujino, Susumu Goyama, Yuki Sugiura, Daichi Inoue, Shuhei Asada, Satoshi Yamasaki, Akiko Matsumoto, Kiyoshi Yamaguchi, Yumiko Isobe, Akiho Tsuchiya, Shiori Shikata, Naru Sato, Hironobu Morinaga, Tomofusa Fukuyama, Yosuke Tanaka, Tsuyoshi Fukushima, Reina Takeda, Keita Yamamoto, Hiroaki Honda, Emi K. Nishimura, Yoichi Furukawa, Tatsuhiro Shibata, Omar Abdel-Wahab, Makoto Suematsu, Toshio Kitamura
Summary: ASXL1 mutations are frequently detected in age-related clonal hematopoiesis (CH), but their mechanism in driving CH remains unclear. The study demonstrates that expression of C-terminal truncated ASXL1 in hematopoietic stem cells leads to Akt de-ubiquitination, activated Akt/mTOR signaling, and aberrant HSC proliferation.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Yoshifumi Mori, Seiji Takashima, Mito Kanatsu-Shinohara, Zheng Yi, Takashi Shinohara
Summary: The study found that Cdc42 is essential for the development of the germline niche, as its deficiency results in a significant reduction in the number of spermatogonial stem cells in the testes, affecting GDNF expression and Sertoli cell development.
Article
Oncology
Jing Tsong Teh, Wan Long Zhu, Christopher B. Newgard, Patrick J. Casey, Mei Wang
MOLECULAR CANCER THERAPEUTICS
(2019)
Review
Oncology
Kanjoormana Aryan Manu, Pham Hong Anh Cao, Tin Fan Chai, Patrick J. Casey, Mei Wang
Article
Biochemistry & Molecular Biology
Wei Kiang Lim, Xiaoran Chai, Sujoy Ghosh, Debleena Ray, Mei Wang, Suhail Ahmed Kabeer Rasheed, Patrick J. Casey
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Review
Cell Biology
Ufuk Degirmenci, Mei Wang, Jiancheng Hu
Article
Oncology
Hiu Yeung Lau, Jingyi Tang, Patrick J. Casey, Mei Wang
Summary: The anchorage-independent soft-agar colony formation assay has been widely used as a bridge between adherent cell cultures and animal tumor studies to predict the tumorigenicity of cancer cells. However, the extraction of biological macromolecules from cells embedded in soft-agar matrices has been a challenge. New methods have been developed to extract DNA, RNA, and proteins directly from cells grown in soft agar, enabling molecular signaling analysis.
Review
Biochemistry & Molecular Biology
Suhail Ahmed Kabeer Rasheed, Lalitha Vaishnavi Subramanyan, Wei Kiang Lim, Udhaya Kumari Udayappan, Mei Wang, Patrick J. Casey
Summary: G12 proteins, a subfamily of GTP-binding proteins, play important roles in human physiology by linking specific cell surface GPCRs to downstream signaling molecules. Studies have shown that increased expression and signaling of G alpha 12 and G alpha 13 are associated with tumorigenesis and tumor progression in multiple cancer types. Targeting G alpha 12/13 signaling may provide a new strategy to improve therapeutic outcomes in solid tumors.
Article
Biology
Jingyi Tang, Patrick J. Casey, Mei Wang
Summary: DNA damage has a dual impact on cancer cells: inducing genomic instability to promote cancer development while compromising proliferation and survival. Inhibiting ICMT reduces MAPK signaling activity, impairs DNA damage repair machinery, leading to cell cycle arrest and apoptosis in cancer cells. ICMT inhibition transforms cancer cells into a BRCA-like state, sensitizing them to PARP inhibitor and other DNA-damaging agents, particularly in anchorage-independent or in vivo conditions.
LIFE SCIENCE ALLIANCE
(2021)
Review
Oncology
Anju Jose, Maria Grazia Bavetta, Erika Martinelli, Fabrizio Bronte, Emilio Francesco Giunta, Kanjoormana Aryan Manu
Summary: Hepatocellular carcinoma is the most common primary liver cancer in patients with liver cirrhosis. Recent advances in understanding its molecular mechanisms and staging have led to the development of new therapies, including targeted therapy and immunotherapy, which have shown promising results, particularly in advanced cases.
JOURNAL OF ONCOLOGY
(2022)
Review
Oncology
Aswathy R. Devan, Bhagyalakshmi Nair, Manu Kanjoormana Aryan, Vijayastelar B. Liju, Joel Joy Koshy, Bijo Mathew, Arun Valsan, Hoon Kim, Lekshmi R. Nath
Summary: Hepatocellular carcinoma (HCC), an inflammation-linked cancer with an immunosuppressive tumor microenvironment, often experiences recurrence within 5 years after curative treatment. Tumor size, number, and histological features are considered risk factors, but there is no effective recurrence marker. Recent studies have highlighted the importance of analyzing the immune contexture in predicting treatment response, prognosis, and recurrence. This review focuses on the prognostic value of immune factors in HCC recurrence after curative treatment and discusses potential immunotherapeutic interventions to prevent tumor recurrence.