Expression analysis of defense-related genes in cucumber (Cucumis sativusL.) againstPhytophthora melonis

Phytophthora melonisis one of the most destructive cucumber disease, causing severe economic losses in the globe. Despite intense research efforts made in the past years, no permanent cure currently exists for this disease. With the aim to understand the molecular mechanisms of defense againstP. melonis, root collars and leaves of four cucumber genotypes consisting of resistant Ramezz; moderately resistant Baby, and very susceptible Mini 6-23 and Extrem, were monitored for quantitative gene expression analysis of the five antifungal and/or anti-oomycete genes (CsWRKY20,CsLecRK6.1,PR3,PR1-1aandLOX1), at three points after inoculation withP. melonis. The gene expression analysis indicated,P. melonisstrongly enhanced the expression of these genes after inoculation, in both the leaves and root collars. Further, not only the transcript levels of these genes were significantly higher in resistant and moderately resistance genotypes, but also the time point of the highest relative expression ratio for the five genes was different in the four cucumber genotypes.CsWRKY20andPR3showed the maximum expression in Ramezz at 48 h post inoculation (hpi) whileCsLecRK6.1, andLOX1showed the highest expression at 72 hpi. In addition,PR1-1ashowed the maximum expression in the Baby at 72 hpi. Root collars responded faster than leaves, and some responses were more strongly up-regulated in root collars than in leaves. The genes found to be involved in disease resistance in two different organs of cucumber after pathogen infection. The results suggest that increased expression of these genes led to activation of defense pathways, and could be responsible for a reducedP. meloniscolonization capacity in Ramezz and Baby. Overall, this work represents a valuable resource for future functional genomics studies to unravel molecular mechanisms ofCucumis sativus-P. melonisinteraction.