Article
Oncology
Da Qin, Yu Zhao, Qingdong Guo, Shengtao Zhu, Shutian Zhang, Li Min
Summary: The study proposed a RNA ratio-based algorithm to quantify EVs-derived RNAs in PDAC and developed a promising prediction model that could effectively differentiate PDAC patients from noncancerous controls.
Article
Chemistry, Analytical
Lianhua Dong, Junbo Zhou, Chunyan Niu, Quanyi Wang, Yang Pan, Sitong Sheng, Xia Wang, Yongzhuo Zhang, Jiayi Yang, Manqing Liu, Yang Zhao, Xiaoying Zhang, Tao Zhu, Tao Peng, Jie Xie, Yunhua Gao, Di Wang, Xinhua Dai, Xiang Fang
Summary: The COVID-19 outbreak caused by a novel Coronavirus has spread globally. Current gold standard RT-qPCR for SARS-CoV-2 diagnosis shows varying sensitivity, highlighting the need for more accurate methods. A study on RT-dPCR demonstrated significant improvement in detection sensitivity, particularly for asymptomatic patients, making it a highly accurate method for screening suspected cases.
Article
Plant Sciences
D. Sharma, A. Koul, S. Bhushan, S. Gupta, S. Kaul, M. K. Dhar
Summary: MicroRNAs play a crucial role in regulating metabolic pathways in plants and animals. This study focuses on exploring the role of microRNAs in the biosynthesis of carotenoids in tomatoes. The findings suggest that microRNAs regulate carotenoid synthesis at different stages of tomato fruit development, and the identified microRNAs can be utilized to enhance the production of different carotenoids in plants.
Article
Chemistry, Analytical
Amy Makler, Ramaswamy Narayanan, Waseem Asghar
Summary: Exosomal miRNA may be used for early detection of PDAC and potentially differentiate patients with chronic pancreatitis, neoplasms, and PDAC. The study identified 18 miRNA with strong potential, and 7 mature miRs showed statistical significance in exosomal RNA.
Review
Oncology
Ryan McGowan, Aine Sally, Anthony McCabe, Brian Michael Moran, Karen Finn
Summary: Pancreatic ductal adenocarcinoma (PDAC) is an aggressive and deadly cancer often diagnosed at a late stage due to nonspecific symptoms and a lack of reliable biomarkers. This review highlights the potential use of circulating nucleic acid-based biomarkers for early detection and monitoring of PDAC. However, the lack of prognostic biomarkers remains a challenge for assessing treatment efficacy.
Article
Biotechnology & Applied Microbiology
Cheng Wang, Jianhua Wang, Wenjing Cui, Yongkang Liu, Hao Zhou, Yajie Wang, Xin Chen, Xiao Chen, Zhongqiu Wang
Summary: The study identified miRNA-1226-3p as a potential biomarker for detecting PDAC and predicting tumor invasion or metastases. Experimental results demonstrated that miRNA-1226-3p can inhibit proliferation, promote apoptosis, and suppress migration of PDAC cells.
ONCOTARGETS AND THERAPY
(2021)
Article
Chemistry, Multidisciplinary
Xander F. van Kooten, Yana Rozevsky, Yulia Marom, Efrat Ben Sadeh, Amit Meller
Summary: Most RNA-based COVID-19 diagnostic methods use enzymatic amplification for high sensitivity but rely on arbitrary thresholding. This study introduces a solid-state nanopore sensing technology for label-free quantification of SARS-CoV-2 RNA in clinical nasal swab samples. The method allows for ratiometric quantification of RNA abundance through single-molecule counting and length-based nanopore identification of dsDNA. The results show that this approach can provide quantitative positive outcomes in clinical samples classified as negative by RT-qPCR.
Article
Biotechnology & Applied Microbiology
Minji Kim, Karen Shapiro, Veronica B. Rajal, Andrea Packham, Beatriz Aguilar, Lezlie Rueda, Stefan Wuertz
Summary: The study evaluated the efficacy of RT-qPCR for selective detection of viable protozoan pathogens in spinach. Among the methods tested, RT-qPCR was more effective in discriminating viable from inactivated pathogens. The application of viability methods on leafy greens can be adopted by the produce industry and regulatory agencies for screening purposes.
Review
Cell Biology
Daniel Zucha, Mikael Kubista, Lukas Valihrach
Summary: Reverse transcription quantitative PCR (RT-qPCR) is a valuable tool for understanding gene expression, particularly in single-cell analysis. This article discusses the limitations of single-cell collection methods, emphasizes the importance of reverse transcription, provides recommendations for preamplification and primer design, and summarizes essential data processing steps. The detailed protocol in the appendix allows any researcher to conduct high-standard scRT-qPCR measurements.
Review
Pharmacology & Pharmacy
Eun-Jeong Won, Hyeji Park, Tae-Jong Yoon, Young-Seok Cho
Summary: This article summarizes the current advances in using nanomedicine-based RNAi therapy and CRISPR/Cas9 genome editing for the treatment of PDAC. Continued research on the PDAC tumor microenvironment and gene therapy will improve patient outcomes.
Review
Biochemistry & Molecular Biology
I. Gede Putu Supadmanaba, Giulia Mantini, Ornella Randazzo, Mjriam Capula, Ittai B. Muller, Stella Cascioferro, Patrizia Diana, Godefridus J. Peters, Elisa Giovannetti
Summary: Pancreatic ductal adenocarcinoma (PDAC) is a deadly cancer with late diagnosis and chemoresistance, but studies on the interaction between splicing and miRNA offer new possibilities for innovative treatments by targeting therapeutic targets and biomarkers.
Article
Biochemistry & Molecular Biology
Liwei Cao, Chen Huang, Daniel Cui Zhou, Yingwei Hu, T. Mamie Lih, Sara R. Savage, Karsten Krug, David J. Clark, Michael Schnaubelt, Lijun Chen, Felipe da Veiga Leprevost, Rodrigo Vargas Eguez, Weiming Yang, Jianbo Pan, Bo Wen, Yongchao Dou, Wen Jiang, Yuxing Liao, Zhiao Shi, Nadezhda Terekhanova, Song Cao, Rita Jui-Hsien Lu, Yize Li, Ruiyang Liu, Houxiang Zhu, Peter Ronning, Yige Wu, Matthew A. Wyczalkowski, Hariharan Easwaran, Ludmila Danilova, Arvind Singh Mer, Seungyeul Yoo, Joshua M. Wang, Wenke Liu, Benjamin Haibe-Kains, Mathangi Thiagarajan, Scott D. Jewell, Galen Hostetter, Chelsea J. Newton, Qing Kay Li, Michael H. Roehr, David Fenyo, Pei Wang, Alexey Nesvizhskii, D. R. Mani, Gilbert S. Omenn, Emily S. Boja, Mehdi Mesri, Ana Robles, Henry Rodriguez, Oliver F. Bathe, Daniel W. Chan, Ralph H. Hruban, Li Ding, Bing Zhang, Hui Zhang
Summary: This study conducted comprehensive proteogenomic analysis of PDAC to understand the molecular alterations that drive oncogenesis. Multiple analyses were performed on tissues from patients, providing valuable resources for early detection and identification of therapeutic targets.
Review
Biochemistry & Molecular Biology
Nausika Betriu, Juan Bertran-Mas, Anna Andreeva, Carlos E. Semino
Summary: Syndecans, a subfamily of proteoglycans, play critical roles in various physiological processes and have implications in disease progression. Their interactions with other macromolecules contribute to normal cellular functions and disease pathogenesis.
Article
Entomology
Kirsten Warcup, Alex S. Torson, George D. Yocum
Summary: "Research on Sex Determination Methods of Colorado Potato Beetles" used publicly available RNA-seq data to select 5 candidate genes for sex-specific markers in adult Colorado potato beetles. The study confirmed that these 5 marker candidates exhibit a sex-specific expression pattern and can be used as PCR markers for sex determination. This method allows for simple sex detection using cDNA as the template and assigning sex to RNA-seq samples post hoc.
JOURNAL OF INSECT SCIENCE
(2023)
Article
Biochemical Research Methods
Xuebing Chen, Hui Xu, Bofeng Zhu
Summary: Inferring the tissue origin types of forensic-relevant body fluids is crucial for reconstructing a crime scene, but accurately identifying different kinds of body fluids remains challenging. This study developed a combined mRNA and miRNA system and applied it to identify five common types of body fluids, demonstrating its advantages in human body fluid identification, especially for mixed samples.
Article
Biochemistry & Molecular Biology
Martina Benesova, Katerina Trejbalova, Dana Kucerova, Zdenka Vernerova, Tomas Hron, Arpad Szabo, Rachel Amouroux, Petr Klezl, Petra Hajkova, Jiri Hejnar
MOLECULAR CARCINOGENESIS
(2017)
Article
Oncology
Stepan Novak, Michal Kolar, Arpad Szabo, Zdena Vernerova, Lukas Lacina, Hynek Strnad, Jana Sachova, Miluse Hradilova, Jan Havranek, Michal Spanko, Matus Coma, Lukas Urban, Miriam Kanuchova, Nikola Melegova, Robert Gurlich, Josef Dvorak, Karel Smetana, Peter Gal, Pavol Szabo
Summary: PDAC cell lines exhibit various responses to conditioned media derived from CAFs, with Panc-1 cells showing the most aggressive behavior and PaTu-8902 cells being inhibited. The heterogeneity in response highlights the complex tumor-stromal interactions that contribute to the aggressive behavior of PDAC.
CANCER GENOMICS & PROTEOMICS
(2021)
Review
Medicine, Research & Experimental
Marian Liberko, Katarina Kolostova, Arpad Szabo, Robert Gurlich, Martin Oliverius, Renata Soumarova
Summary: Pancreatic ductal adenocarcinoma is a disease with increasing incidence and poor prognosis, despite aggressive multimodal treatments. There is currently a lack of prognostic and/or predictive biomarkers available to identify and tailor treatment based on risk factors.
Article
Oncology
A. Szabo, R. Gurlich, M. Liberko, R. Soumarova, Z. Vernerova, V Mandys, A. Popov
Article
Medicine, General & Internal
I Hoffmanova, R. Gurlich, V Janik, A. Szabo, Z. Vernerova
BRATISLAVA MEDICAL JOURNAL-BRATISLAVSKE LEKARSKE LISTY
(2016)
Article
Cardiac & Cardiovascular Systems
Kamila Blechova, Jiri Knot, Arpad Szabo