Journal
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
Volume 31, Issue 12, Pages 2547-2552Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jasms.0c00128
Keywords
Mass Spectrometry Imaging; Arbitrary Region of Interest; MALDESI; Vision-System
Funding
- NIH [R01GM087964, R01AI111891]
- North Carolina State University
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A vision-system driven platform, RastirX, has been constructed for mass spectrometry imaging (MSI) of arbitrary two-dimensional patterns. The user identifies a region of interest (ROI) by drawing on a live video image of the sample with the computer mouse. Motion commands are automatically generated to move the sample to acquire scan data for the pixels in the ROI. Synchronization of sample stage motion with laser firing and mass spectrometer (MS) scan acquisition is fully automated. RastirX saves a co-registered optical image and the scan location information needed to convert raw MS data into imzML format. Imaging an arbitrarily shaped ROI instead of the minimal enclosing rectangle reduces contamination from off-sample material and significantly reduces acquisition time.
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