Article
Engineering, Biomedical
Yao Liu, Xiaonong Zhang, Chunsheng Xiao, Bin Liu
Summary: Peripheral nerve injury (PNI) is a complex disease that commonly affects young adults and has limited treatment options and poor clinical outcomes. Tissue-engineered hydrogels have shown promising results in promoting neural tissue regeneration and recovery of impaired neural function. This review discusses recent advances and various therapeutic strategies of hydrogels in peripheral nerve repair, as well as the prospects and challenges in their application for PNI treatment.
MATERIALS TODAY BIO
(2023)
Article
Engineering, Biomedical
Hongkui Wang, Ping Zhang, Panjian Lu, Xiaodong Cai, Gang Wang, Xi Xu, Ying Liu, Tianyi Huang, Meiyuan Li, Tianmei Qian, Hui Zhu, Chengbin Xue
Summary: In this study, a subcutaneously pre-vascularized tissue-engineered nerve graft (TENG) was developed using a vascular endothelial growth factor-induced host vascular network, chitosan nerve conduit, and inserted silk fibroin fibers. The functionality of regenerated blood vessels was confirmed through various imaging techniques and 3D reconstruction. Pre-vascularized TENG facilitated rapid vascular inosculation and enhanced the migration of vascular endothelial cells (VECs) and Schwann cells (SCs), promoting axonal regrowth and remyelination. The transplantation of pre-vascularized TENG resulted in the recovery of electrophysiological function and prevention of muscle atrophy.
MATERIALS TODAY BIO
(2023)
Article
Engineering, Biomedical
Eric W. Atkinson, Cary A. Kuliasha, Mary Kasper, Abbas Furniturewalla, Alexander S. Lim, Ladan Jiracek-Sapieha, Alexis Brake, Anne Gormaley, Victor Rivera-Llabres, Ishita Singh, Benjamin Spearman, Carlos M. Rinaldi-Ramos, Christine E. Schmidt, Jack W. Judy, Kevin J. Otto
Summary: This study investigated the in vivo functionality of the MARTEENI device as a potential approach to improving current issues in peripheral nerve interfaces. The results showed that the MARTEENI device exhibited channel-isolated activity in acute, chronic, and terminal experiments, with regenerating axons surrounding the device threads. These findings suggest that the MARTEENI device is a promising approach for interfacing with peripheral nerves.
JOURNAL OF NEURAL ENGINEERING
(2022)
Review
Biotechnology & Applied Microbiology
Rebecca Powell, Despoina Eleftheriadou, Simon Kellaway, James B. Phillips
Summary: This review discusses the physical processes by which natural biomaterials mimic the function of the extracellular matrix and regulate cellular behavior, as well as highlights representative cases of controllable cell microenvironments developed through the combination of cell biology and tissue engineering principles.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Neurosciences
Qisong Su, Moussa Ide Nasser, Jiaming He, Gang Deng, Qing Ouyang, Donglin Zhuang, Yuzhi Deng, Haoyun Hu, Nanbo Liu, Zhetao Li, Ping Zhu, Ge Li
Summary: Compared to the central nervous system, the peripheral nervous system has a greater capacity for regeneration due to the plasticity of Schwann cells (SCs). This review examines the potential of using genetically modified SCs or tissue-engineered nerves for the repair of peripheral nervous injuries (PNIs). The therapeutic effects of these strategies resemble autologous transplantation, which is currently the gold standard for PNI repair.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Cami C. Rowan, Oliver Graudejus, Timothy M. Otchr
Summary: This study presents a novel peripheral nerve interface (PNI) that breaks the tradeoff between selectivity and invasiveness by interfacing with small nerves, demonstrating electromechanical robustness and reliable neural activity recording and stimulation capabilities. The new PNI design could also guide the development of other types of PNIs for optical, thermal, ultrasonic, or chemical applications.
Article
Engineering, Biomedical
Melissa L. D. Rayner, Adam G. E. Day, Kulraj S. Bhangra, John Sinden, James B. Phillips
Summary: This study provides evidence supporting the effectiveness of using engineered neural tissue (EngNT) as a replacement for nerve autografts, with functional regeneration assessed through histological and electrophysiological outcome measures demonstrating equivalent performance.
ACTA BIOMATERIALIA
(2021)
Article
Neurosciences
Meiyuan Li, Xiyang Cheng, Shuyue Feng, Hui Zhu, Panjian Lu, Ping Zhang, Xiaodong Cai, Pingping Qiao, Xiaosong Gu, Gang Wang, Chengbin Xue, Hongkui Wang
Summary: Prevascularization strategies are important in tissue engineering. Skin precursor-derived Schwann cells (SKP-SCs) have shown potential in constructing prevascularized tissue-engineered peripheral nerves. Silk fibroin scaffolds seeded with SKP-SCs were prevascularized through implantation and assembled with a chitosan conduit. SKP-SCs expressed pro-angiogenic factors and significantly accelerated prevascularization compared to VEGF, showing superior short-term nerve regeneration.
Article
Cell & Tissue Engineering
Omar A. Selim, Saad Lakhani, Swati Midha, Afshin Mosahebi, Deepak M. Kalaskar
Summary: Complex nerve injuries, including critical-sized gaps and nerve bifurcations, are challenging to manage and require novel solutions. The current peripheral nerve repair market is large and growing, highlighting the need for effective nerve substitutes. Advanced 3D printing technologies show promise in developing patient-specific and biomimetic nerve repair solutions.
TISSUE ENGINEERING PART B-REVIEWS
(2022)
Article
Engineering, Biomedical
Papon Muangsanit, Victoria Roberton, Eleni Costa, James B. Phillips
Summary: Vascularisation is crucial for nerve tissue engineering to support long-term survival of implanted cells. Constructs containing endothelial cells have shown to promote axonal regeneration and vascularisation across peripheral nerve gaps.
ACTA BIOMATERIALIA
(2021)
Article
Chemistry, Multidisciplinary
Zhi Yao, Weihao Yuan, Jiankun Xu, Wenxue Tong, Jie Mi, Pak-Cheong Ho, Dick Ho Kiu Chow, Ye Li, Hao Yao, Xu Li, Shunxiang Xu, Jiaxin Guo, Qingtang Zhu, Liming Bian, Ling Qin
Summary: In this study, an injectable hydrogel based on bisphosphonate was developed to sustainably release magnesium ions for peripheral nerve regeneration. The experimental results showed that magnesium ions promoted neurite outgrowth in a concentration-dependent manner by activating the PI3K/Akt signaling pathway and Sema5b. In a rat nerve defect model, filling the polycaprolactone conduit with magnesium-releasing hydrogel significantly enhanced axon regeneration and remyelination, leading to improved functional recovery.
Article
Engineering, Biomedical
Simon C. Kellaway, Victoria Roberton, Joshua N. Jones, Rabea Loczenski, James B. Phillips, Lisa J. White
Summary: Incorporating dECMh derived from bone, liver, and small intestinal tissues can promote neurite extension and axonal regeneration, providing an alternative to current collagen I hydrogel.
ACTA BIOMATERIALIA
(2023)
Review
Chemistry, Multidisciplinary
Wufei Dai, Yating Yang, Yumin Yang, Wei Liu
Summary: Tissue-engineered nerve conduits, using both natural and synthetic materials, have been employed to treat peripheral nerve defects and promote nerve regeneration. In addition to traditional advantages, such as good biocompatibility and controllable degradation, these conduits have been enhanced with properties like biomimetic surface topography, extracellular matrix components, neurotrophic factors, and cell seeding.
NANOTECHNOLOGY REVIEWS
(2021)
Article
Biotechnology & Applied Microbiology
Lorena R. Lizarraga-Valderrama, Giulia Ronchi, Rinat Nigmatullin, Federica Fregnan, Pooja Basnett, Alexandra Paxinou, Stefano Geuna, Ipsita Roy
Summary: The study demonstrated that PHA-NGCs are highly promising in promoting peripheral nerve regeneration, showing comparable outcomes to autografts in functional evaluation and nerve morphology.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2021)
Article
Cell & Tissue Engineering
Jian Zhang, Chaochao Li, Fanqi Meng, Yanjun Guan, Tieyuan Zhang, Boyao Yang, Zhiqi Ren, Xiuzhi Liu, Dongdong Li, Jinjuan Zhao, Jie Zhao, Yu Wang, Jiang Peng
Summary: The study found that tissue-engineered microtissue is an effective strategy for stem cell culture and therapy in nerve tissue engineering. Microtissues can secrete more nerve regeneration related cytokines to promote cell proliferation and axon growth, and can effectively repair nerve defects.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Engineering, Biomedical
Nikunj K. Agrawal, Peter Allen, Young Hye Song, Rebecca A. Wachs, Yan Du, Andrew D. Ellington, Christine E. Schmidt
ACTA BIOMATERIALIA
(2020)
Article
Engineering, Biomedical
Benjamin S. Spearman, Nikunj K. Agrawal, Andres Rubiano, Chelsey S. Simmons, Sahba Mobini, Christine E. Schmidt
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2020)
Article
Engineering, Biomedical
Sahba Mobini, Cary A. Kuliasha, Zachary A. Siders, Nicole A. Bohmann, Syed-Mustafa Jamal, Jack W. Judy, Christine E. Schmidt, Anthony B. Brennan
Summary: The chronic reliability of bioelectronic neural interfaces has been challenged by foreign body reactions, but engineered microtopographies, such as Sharklet (TM), have shown potential in manipulating cellular responses to the implanted device. While surface patterning is a strong physical tool for modulating cell behavior, responses to micropatterns are highly dependent on the cell type, with Schwann cells found to be more metabolically active and aligned than fibroblasts when compared between the same pattern.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Polymer Science
Mark D. Ashton, Isabel C. Appen, Melike Firlak, Naomi E. Stanhope, Christine E. Schmidt, William R. Eisenstadt, Byul Hur, John G. Hardy
Summary: The development of stimuli-responsive drug delivery systems provides significant opportunities for industrial innovation, enabling spatiotemporal control of drug release. The wireless controller device has the potential to revolutionize the field of biomaterial design and application.
POLYMER INTERNATIONAL
(2021)
Review
Engineering, Biomedical
Mary Kasper, Curt Deister, Florence Beck, Christine E. Schmidt
ADVANCED HEALTHCARE MATERIALS
(2020)
Article
Cardiac & Cardiovascular Systems
Deanna Bousalis, Christopher S. Lacko, Nora Hlavac, Fariz Alkassis, Rebecca A. Wachs, Sahba Mobini, Christine E. Schmidt, Hideko Kasahara
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2020)
Article
Materials Science, Biomaterials
Tran B. Ngo, Benjamin S. Spearman, Nora Hlavac, Christine E. Schmidt
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2020)
Review
Engineering, Biomedical
Eleana Manousiouthakis, Junggeon Park, John G. Hardy, Jae Young Lee, Christine E. Schmidt
Summary: This review explores the applications of conductive and electroactive materials in neural tissue regeneration, which is of significant interest to a broad readership and particularly relevant to scientists, engineers, and clinicians in academia and industry who develop innovative medical devices for tissue engineering and regenerative medicine.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Nora Hlavac, Dillon T. Seroski, Nikunj K. Agrawal, Leilani Astrab, Renjie Liu, Gregory A. Hudalla, Christine E. Schmidt
Summary: The study explored the functional and in vitro application of engineered ChABC fused to galectin-3, demonstrating extended release time and enhanced enzymatic activity compared to wild type ChABC when incorporated in hydrogels. These stabilized ChABC-Gal3 oligomers combined with HA-based scaffolds show promising potential for neural regeneration applications.
JOURNAL OF NEURAL ENGINEERING
(2021)
Article
Engineering, Biomedical
Nora Hlavac, Deanna Bousalis, Raffae N. Ahmad, Emily Pallack, Angelique Vela, Yuan Li, Sahba Mobini, Erin Patrick, Christine E. Schmidt
Summary: This study investigated the impact of low-level electrical stimulation on the regenerative potential of adipose-derived stem cells, revealing that frequency and duration significantly affect the expression of growth factors. Continuous exposure to 1 Hz pulses for 24 hours was found to optimally promote the expression of neural growth factors and induce significant increase in neurite extension.
ANNALS OF BIOMEDICAL ENGINEERING
(2021)
Article
Engineering, Biomedical
Mary Kasper, Bret Ellenbogen, Ryan Hardy, Madison Cydis, Jorge Mojica-Santiago, Abdullah Afridi, Benjamin S. Spearman, Ishita Singh, Cary A. Kuliasha, Eric Atkinson, Kevin J. Otto, Jack W. Judy, Carlos Rinaldi-Ramos, Christine E. Schmidt
Summary: The magnetically aligned regenerative tissue-engineered electronic nerve interface (MARTEENI) combines polyimide threads encapsulated within a magnetically aligned hydrogel scaffold to address concerns over traditional peripheral nerve interfaces. The technology promotes nerve regeneration and increased axon densities, while also attenuating foreign-body responses. Experimental results demonstrate the potential of MARTEENI devices in promoting nerve regeneration and improving nerve interface selectivity.
Article
Engineering, Biomedical
Deanna Bousalis, Michaela W. McCrary, Natalie Vaughn, Nora Hlavac, Ashley Evering, Shruti Kolli, Young Hye Song, Cameron Morley, Thomas Angelini, Christine E. Schmidt
Summary: Damage to the nervous system can lead to severe consequences, but utilizing biomaterial strategies can promote neural regeneration and functional recovery. Injectable hydrogels offer flexibility and growth-permissive properties for neural cells, making them a promising approach for neural tissue engineering.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Cell Biology
Shelby Giza, Jorge A. Mojica-Santiago, Maddalena Parafati, Legrand K. Malany, Don Platt, Christine E. Schmidt, Paul M. Coen, Siobhan Malany
Summary: Microphysiological systems (MPS) incorporating 3D skeletal myobundles are used to study new medical treatments for sarcopenia. In this study, myobundles derived from young, active individuals showed better response to electrical stimulation and increased hypertrophic potential compared to myobundles derived from older sedentary individuals. This research provides a disease model and experimental platform for studying the effects of electrical stimulation on muscle aging and evaluating the efficacy and toxicity of new drugs for sarcopenia.
Article
Materials Science, Biomaterials
Young Hye Song, Mark A. Maynes, Nora Hlavac, Daniel Visosevic, Kaitlyn O. Daramola, Stacy L. Porvasnik, Christine E. Schmidt
Summary: The article introduces a method for decellularizing lung tissue using apoptosis, which can be widely utilized in both regenerative medicine and disease modeling.
BIOMATERIALS SCIENCE
(2021)
Review
Materials Science, Multidisciplinary
N. Hlavac, M. Kasper, C. E. Schmidt
MATERIALS TODAY ADVANCES
(2020)
Article
Biochemical Research Methods
Aline Silva da Cruz, Maria Margarida Drehmer, Wagner Baetas-da-Cruz, Joao Carlos Machado
Summary: This study quantified microcirculation cerebral blood flow in a rat model of ischemic stroke using ultrasound biomicroscopy and ultrasound contrast agents. The results showed high sensitivity and specificity of this method, making it a valuable tool for preclinical studies.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Christina Dalla, Ivana Jaric, Pavlina Pavlidi, Georgia E. Hodes, Nikolaos Kokras, Anton Bespalov, Martien J. Kas, Thomas Steckler, Mohamed Kabbaj, Hanno Wuerbel, Jordan Marrocco, Jessica Tollkuhn, Rebecca Shansky, Debra Bangasser, Jill B. Becker, Margaret McCarthy, Chantelle Ferland-Beckham
Summary: Many funding agencies have emphasized the importance of considering sex as a biological variable in experimental design to improve the reproducibility and translational relevance of preclinical research. Omitting the female sex from experimental designs in neuroscience and pharmacology can result in biased or limited understanding of disease mechanisms. This article provides methodological considerations for incorporating sex as a biological variable in in vitro and in vivo experiments, including the influence of age and hormone levels, and proposes strategies to enhance methodological rigor and translational relevance in preclinical research.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Wenyu Gu, Dongxu Li, Jia-Hong Gao
Summary: We developed a precise and rapid method for positioning and labelling triaxial OPMs on a wearable magnetoencephalography (MEG) system, improving the efficiency of OPM positioning and labelling.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Kai Lin, Linhang Zhang, Jing Cai, Jiaqi Sun, Wenjie Cui, Guangda Liu
Summary: The article introduces an EEG feature map processing model for emotion recognition, which achieves significantly improved accuracy by fusing EEG information at different spatial scales and introducing a channel attention mechanism.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
John E. Parker, Asier Aristieta, Aryn H. Gittis, Jonathan E. Rubin
Summary: This work presents a toolbox that implements a methodology for automated classification of neural responses based on spike train recordings. The toolbox provides a user-friendly and efficient approach to detect various types of neuronal responses that may not be identified by traditional methods.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Yun Liang, Ke Bo, Sreenivasan Meyyappan, Mingzhou Ding
Summary: This study compared the performance of SVM and CNN on the same datasets and found that CNN achieved consistently higher classification accuracies. The classification accuracies of SVM and CNN were generally not correlated, and the heatmaps derived from them did not overlap significantly.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Antonino Visalli, Maria Montefinese, Giada Viviani, Livio Finos, Antonino Vallesi, Ettore Ambrosini
Summary: This study introduces an analytical strategy that allows the use of mixed-effects models (LMM) in mass univariate analyses of EEG data. The proposed method overcomes the computational costs and shows excellent performance properties, making it increasingly important in the field of neuroscience.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Xavier Cano-Ferrer, Alexandra Tran -Van -Minh, Ede Rancz
Summary: This study developed a novel rotation platform for studying neural processes and spatial navigation. The platform is modular, affordable, and easy to build, and can be driven by the experimenter or animal movement. The research demonstrated the utility of the platform, which combines the benefits of head fixation and intact vestibular activity.
JOURNAL OF NEUROSCIENCE METHODS
(2024)