Article
Biochemistry & Molecular Biology
G. Demyashkin, E. Kogan, D. Boldyrev, T. Demura, A. Tyatyushkina, E. Annenkova, K. Semenov, I. Zorin, A. Zverev
Summary: After the outbreak of COVID-19, scientists have gained a better understanding of the disease's pathogenesis and its impact on various organs and tissues. However, the effect of the virus on fertility remains unclear. Previous studies have provided conflicting results, and there is no evidence of a direct effect on the male reproductive organs. Further research is needed to confirm if the testicles are a target organ for SARS-CoV-2.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biotechnology & Applied Microbiology
Nikita Sherwani, Neha Singh, Arvind Neral, Jyoti Jaiswal, Tripti Nagaria, Onkar Khandwal
Summary: This study explores placental pathology in mothers infected with COVID-19 and finds that congenital transmission of SARS-CoV-2 appears to be rare. The protective properties of the placental barrier against viral infections from mother to newborn remain unclear, but the abundance of ACE-2 on the placental surface may contribute to adverse outcomes during pregnancy.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Galyah Alhamid, Huseyin Tombuloglu, Ebtesam Al-Suhaimi
Summary: The reverse-transcription loop-mediated isothermal amplification (RT-LAMP) is a cheaper and faster testing alternative for detecting SARS-CoV-2. We developed colorimetric and fluorometric RT-LAMP assays using five LAMP primers instead of six to overcome misamplifications and verified their performance with the gold-standard RT-PCR technique. The E-ID1 primer set, including five primers, performed superbly on both colorimetric and fluorometric assays, with high sensitivity, specificity, and accuracy.
SCIENTIFIC REPORTS
(2023)
Article
Pathology
Erkan Ozcan, Serap Yavuzer, Betul Borku Uysal, Mehmet Sami Islamoglu, Hande Ikitimur, Omer Faruk Unal, Yunus Emre Akpinar, Serhat Seyhan, Suna Koc, Hakan Yavuzer, Mahir Cengiz
Summary: The study found that among suspected COVID-19 patients with pneumonia symptoms and lung infiltrates consistent with COVID-19 on CT imaging, there were no significant differences in symptoms, physical examination, total CT scores, hospitalization duration, ICU requirement, and mortality rate between RT-PCR-positive and RT-PCR-negative patients. However, PCR-positive patients appeared to require more specific treatments.
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2021)
Article
Multidisciplinary Sciences
Jean-Baptiste Lascarrou, Gwenhael Colin, Aurelie Le Thuaut, Nicolas Serck, Mickael Ohana, Bertrand Sauneuf, Guillaume Geri, Jean-Baptiste Mesland, Gaetane Ribeyre, Claire Hussenet, Anne Sophie Boureau, Thomas Gille
Summary: In a multicenter case-control study on COVID-19 patients, it was found that patients with symptoms of headache and fatigue/malaise were more likely to have a false-negative result in their first RT-PCR test. Conversely, patients with elevated platelet and C-reactive protein levels were at a higher risk of a false-negative first RT-PCR test result. This highlights the importance of evaluating strategies involving serial RT-PCR testing for COVID-19 diagnosis.
SCIENTIFIC REPORTS
(2021)
Review
Environmental Sciences
Md. Maniruzzaman, Md. Missile Islam, Md. Hazrat Ali, Nobendu Mukerjee, Swastika Maitra, Mohammad Amjad Kamal, Arabinda Ghosh, Melvin A. Castrosanto, Athanasios Alexiou, Ghulam Md. Ashraf, Priti Tagde, Md. Habibur Rahman
Summary: COVID-19 has become a pandemic that disrupts everyone's routine and balanced way of life worldwide. Multiple COVID-19 vaccines have been developed, but the ability of the SARS-CoV-2 virus to mutate poses challenges. It is crucial to spread vaccination programs and prevent the spread of the virus by identifying and isolating infected patients. Additionally, COVID-19 testing methods face issues such as cost, time consumption, and qualified personnel and facilities requirements.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Environmental Sciences
Olfa Baccari, Mohamed Barkallah, Jihen Elleuch, Nourelhouda Ben Ayed, Amel Chtourou, Hela Karray-Hakim, Adenene Hammami, Philippe Michaud, Imen Fendri, Slim Abdelkafi
Summary: Parachlamydia acanthamoebae, a potential respiratory pathogen, was detected using a TaqMan-PCR assay that showed good accuracy and reproducibility. This diagnostic tool can be used for large-scale screening and during outbreaks.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Review
Biochemical Research Methods
Basant Giri, Shishir Pandey, Retina Shrestha, Krisha Pokharel, Frances S. Ligler, Bhanu B. Neupane
Summary: In the recent COVID-19 pandemic, various diagnostic methods have been developed for detecting the virus, with factors such as throughput, infrastructure requirements, and analytical performance varying between methods. Selecting a reliable and rapid diagnostic method is crucial for making appropriate decisions and implementing effective public health interventions.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Nanoscience & Nanotechnology
Alexis Scholtz, Anuradha Ramoji, Anja Silge, Jakob R. Jansson, Ian G. de Moura, Jurgen Popp, Jakub P. Sram, Andrea M. Armani
Summary: The emergence of SARS-CoV-2 in winter of 2020 led to rapid advancements in diagnostic technologies to monitor and combat the spread of the virus, ultimately reducing its impact on society. These developments have also laid the foundation for new platform technologies, which will continue to be crucial in addressing future epidemics.
Article
Immunology
Zhengtu Li, Yinhu Li, Lingdan Chen, Shaoqiang Li, Le Yu, Airu Zhu, Feng Yang, Qian Jiang, Liyan Chen, Jincun Zhao, Wenju Lu, Nanshan Zhong, Feng Ye
Summary: This study reported a confirmed case of SARS-CoV-2 pneumonia with negative routine RT-PCR test, which was ultimately diagnosed by nanopore sequencing and SARS-CoV-2 antibody detection. Gene variations in SARS-CoV-2 were also identified. The findings suggest the presence of false-negative results in routine RT-PCR diagnosis, especially in the context of virus variations, with nanopore pathogen sequencing and antibody detection being effective in clinical diagnosis.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Immunology
Norihito Kaku, Kenji Ota, Daisuke Sasaki, Norihiko Akamatsu, Naoki Uno, Kei Sakamoto, Kosuke Kosai, Hiroo Hasegawa, Koichi Izumikawa, Hiroshi Mukae, Katsunori Yanagihara
Summary: This retrospective study evaluated stored nasopharyngeal swab samples from Japanese patients with influenza-like illness during the 2019/2020 season, aiming to determine whether COVID-19 had spread in the community before the first confirmed case. The study found no large-scale spread of COVID-19 in the community before the first confirmed case in Nagasaki, Japan.
MICROBES AND INFECTION
(2021)
Article
Virology
Nicholas M. Adams, Mindy Leelawong, Alison Benton, Criziel Quinn, Frederick R. Haselton, Jonathan E. Schmitz
Summary: The COVID-19 pandemic has increased the demand for molecular diagnostics, but access to RNA extraction reagents can be a bottleneck. A study introduced an unextracted modification for qRT-PCR, which showed slightly decreased analytic sensitivity but high categorical agreement with extracted results. This suggests that laboratories could consider unextracted qRT-PCR protocols as an option to overcome supply limitations.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Infectious Diseases
Heta Parmar, Margaret Montovano, Padmapriya Banada, Sri Ram Pentakota, Stephanie Shiau, Zhongjie Ma, Kaheerman Saibire, Abby Chopoorian, Michael O'Shaughnessy, Mitchell Hirsch, Priyanshi Jain, Gaiane Demirdjian, Magali Karagueuzian, Thomas Robin, Michael Salvati, Bhavana Patel, David Alland, Yingda L. Xie
Summary: This study suggests a high likelihood of acute COVID-19 among RT-PCR negative patients with typical signs/symptoms, but a common omission of COVID-19 therapies among these patients.
BMC INFECTIOUS DISEASES
(2022)
Article
Virology
Yakup Artik, Alp B. Cosgun, Nevra P. Cesur, Nedret Hizel, Yavuz Uyar, Haydar Sur, Alp Ayan
Summary: The present study evaluates the effectiveness of LAMP method for rapid screening diagnostic test of COVID-19 and compares it with the gold standard q-RT-PCR. The results show that LAMP method performs similarly to q-RT-PCR in some cases and offers the advantages of speed and cost-effectiveness.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Medicine, General & Internal
Mallika Lavania, Madhuri S. Joshi, Sujata S. Ranshing, Varsha A. Potdar, Manohar Shinde, Nutan Chavan, Santosh M. Jadhav, Prasad Sarkale, Sreelekshmy Mohandas, Pradeep M. Sawant, Sanjaykumar Tikute, Vikram Padbidri, Sampada Patwardhan, Rohan Kate
Summary: The main routes of transmission for SARS-CoV-2 are through respiratory pathways and close contact. While the possibility of fecal-oral transmission has been supported by some published literature, it is not yet clear how fecal viral load is related to disease severity. This study investigated SARS-CoV-2 shedding in fecal samples of COVID-19 patients and found that about 62% of patients tested positive for SARS-CoV-2 RNA in their fecal specimens. However, this positivity was not related to gastrointestinal symptoms or disease severity.
FRONTIERS IN MEDICINE
(2022)
Article
Oncology
Man Li, Shi-Ming Chen, Chen Chen, Zi-Xiong Zhang, Meng-Yuan Dai, Lei-Bo Zhang, Shui-Bin Wang, Qiong Dai, Ze-Zhang Tao
MOLECULAR MEDICINE REPORTS
(2015)
Article
Oncology
Fen Li, Chen Chen, Shi-Ming Chen, Bo-Kui Xiao, Ze-Zhang Tao
Article
Medicine, Research & Experimental
You Zou, Yan Wang, Shui-Bin Wang, Yong-Gang Kong, Yu Xu, Ze-Zhang Tao, Shi-Ming Chen
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2016)
Article
Oncology
You Zou, Fang Fang, Yong-Jun Ding, Meng-Yuan Dai, Xing Yi, Chen Chen, Ze-Zhang Tao, Shi-Ming Chen
MOLECULAR MEDICINE REPORTS
(2016)
Article
Oncology
Meng-Yuan Dai, Yan Wang, Chen Chen, Fen Li, Bo-Kui Xiao, Shi-Ming Chen, Ze-Zhang Tao
Article
Oncology
Mao-Ling Huang, You Zou, Rui Yang, Yang Jiang, Jian-Fei Sheng, Ji-Bo Han, Yong-Gang Kong, Ze-Zhang Tao, Shi-Ming Chen
EXPERIMENTAL CELL RESEARCH
(2019)
Article
Allergy
Wo-Er Jiao, Jin-Feng Wei, Yong-Gang Kong, Yu Xu, Ze-Zhang Tao, Shi-Ming Chen
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
(2019)
Article
Immunology
Shan Xu, Yong-Gang Kong, Wo-Er Jiao, Rui Yang, Yue-Long Qiao, Yu Xu, Ze-Zhang Tao, Shi-Ming Chen
INTERNATIONAL IMMUNOPHARMACOLOGY
(2019)
Article
Oncology
You Zou, Rui Yang, Mao-Ling Huang, Yong-Gang Kong, Jian-Fei Sheng, Ze-Zhang Tao, Ling Gao, Shi-Ming Chen
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2019)
Article
Cell Biology
Mao-Ling Huang, Cheng-Lin Qi, You Zou, Rui Yang, Yang Jiang, Jian-Fei Sheng, Yong-Gang Kong, Ze-Zhang Tao, Shi-Ming Chen
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2020)
Article
Multidisciplinary Sciences
Jia-Ni Zou, Liu Sun, Bin-Ru Wang, You Zou, Shan Xu, Yong-Jun Ding, Li-Jun Shen, Wen-Cai Huang, Xiao-Jing Jiang, Shi-Ming Chen
Summary: The study using AI-assisted chest HRCT revealed the incidence, characteristics, risk factors, and evolution of pulmonary fibrosis in patients with COVID-19. Patients with severe or critical COVID-19 had a higher risk of pulmonary fibrosis, but with time, most patients showed improvement or resolution of pulmonary fibrosis.
Article
Oncology
Cheng-Lin Qi, Mao-Ling Huang, You Zou, Rui Yang, Yang Jiang, Jian-Fei Sheng, Yong-Gang Kong, Ze-Zhang Tao, Hong-Yan Feng, Qing-Quan Hua, Li-Hong Bu, Shi-Ming Chen
Summary: CENP-N is highly expressed in NPC and is associated with poor prognosis. The IRF2/CENP-N/AKT axis regulates glucose metabolism and cell cycle, promoting proliferation and anti-apoptotic abilities in NPC cells.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Immunology
Jun-Hua Wei, Yue-long Qiao, Shan Xu, You Zou, Hai-Feng Ni, Li-Zhi Wu, Ze-Zhang Tao, Wo-Er Jiao, Shi-Ming Chen
Summary: This study investigated the effect of Notch2 gene knockout in Treg cells on head and neck squamous cell carcinoma (HNSCC) in mice. The results showed that specific knockout of the Notch2 gene in Treg cells significantly decreased tumor volume, reduced the number of Treg cells, inhibited tumor growth, and improved the immune microenvironment in mice.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Oncology
Rui Yang, Ze-Zhang Tao, Mao-Ling Huang, Yong-Fa Zheng, Meng-Yuan Dai, You Zou, Shi-Ming Chen
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2018)