Article
Biochemical Research Methods
Roberto Dallocchio, Barbara Sechi, Alessandro Dessi, Bezhan Chankvetadze, Sergio Cossu, Victor Mamane, Robin Weiss, Patrick Pale, Paola Peluso
Summary: This study investigated the enantioseparation of 2'-(4-pyridyl)- and 2'-(4-hydroxyphenyl)-TCIBPs on various chiral columns, exploring the factors influencing the selectivity between enantiomers, including the polymer backbone and side chain substituent properties. Findings showed that the cellulose and amylose tris(3,5-dimethylphenylcarbamate) influenced the elution order of analytes, highlighting the importance of polysaccharide backbone in enantioseparation.
Article
Biochemistry & Molecular Biology
Mohammadhassan Foroughbakhshfasaei, Mate Dobo, Francisc Boda, Zoltan-Istvan Szabo, Gergo Toth
Summary: The study investigated the enantioseparation of four phthalimide derivatives using different polysaccharide-type stationary phases and polar organic mobile phases. It found that only Chiralcel OJ-H column with MeOH was successful for baseline-separation of all investigated drugs, and observed that enantioselectivity depends on the direction from which the composition of the eluent is approached, regardless of the eluent pair used.
Article
Biochemical Research Methods
Mate Dobo, Mohammadhassan Foroughbakhshfasaei, Peter Horvath, Zoltan-Istvan Szabo, Gergo Toth
Summary: The enantioseparation of four oxazolidinone and one biosimilar thiazolidine derivatives was performed on seven different polysaccharide-type chiral stationary phases, with Amylose-based columns providing the highest enantioselectivities. Replacing an oxygen with a sulfur atom in the backbone of the studied analytes significantly alters the enantiomer recognition mechanism. Additionally, reversal of enantiomer elution order and hysteresis of retention and enantioselectivity were observed, which can be further utilized for fine-tuning chiral separation performance of the studied columns.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Khadidja Addadi, Khaled Sekkoum, Nasser Belboukhari, Zeid A. ALOthman, Ahmed M. Aljuwayid, Mika Sillanpaa, Imran Ali
Summary: In this study, the enantio-separation of three chiral sulfoxide drugs was achieved on a Chiralpak AS-3R column using acetonitrile-water as the mobile phase. The chiral separation was validated properly with LOD and LOQ values of 9.70-14.16 and 29.39-42.91 μg/mL, respectively. The enantiomer elution order was confirmed through experimental and theoretical methods. A simple, fast, reliable, and thermodynamically stable HPLC method was developed for the separation and determination of enantiomer elution order of the chiral sulfoxide drugs.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemical Research Methods
Roberto Dallocchio, Alessandro Dessi, Barbara Sechi, Bezhan Chankvetadze, Giorgi Jibuti, Sergio Cossu, Victor Mamane, Paola Peluso
Summary: In this study, the enantioseparation of 14 planar chiral ferrocenes was investigated using cellulose tris(4-methylbenzoate) (CMB) and cellulose tris(3,5-dimethylphenylcarbamate) (CDMPC) chiral columns. Baseline enantioseparations were achieved for nine analytes with the CMB column, compared to only three with the CDMPC column, under the same elution conditions. The impact of analyte and chiral stationary phase (CSP) structure, as well as mobile phase (MP) polarity, on the enantioseparation was evaluated.
Review
Biochemistry & Molecular Biology
Robert Berkecz, Gabor Nemeti, Antal Peter, Istvan Ilisz
Summary: Chiral compounds play a crucial role in living organisms, making the enantioseparation of compounds a key task in modern analytical chemistry. High-performance liquid chromatography is the most commonly used method for separating chiral compounds.
Article
Chemistry, Analytical
Attila Bajtai, Istvan Ilisz, Robert Berkecz, Ferenc Fulop, Wolfgang Lindner, Antal Peter
Summary: This study investigated the diastereo- and enantioseparation of Cinchona alkaloid analogs using liquid chromatographic and subcritical fluid chromatographic modalities. The Chiralpak IC and IG columns were found to be the best in both modalities. Temperature was identified as a crucial factor in the separation process.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biochemistry & Molecular Biology
Lajos-Attila Papp, Sarolta Krizbai, Mate Dobo, Gabriel Hancu, Zoltan-Istvan Szabo, Gergo Toth
Summary: A novel reversed-phase chiral HPLC method was developed for the enantiopurity control analysis of naproxen. The method utilized a polysaccharide-type chiral stationary phase and different mobile phase compositions to optimize the enantiomer separation.
Article
Biochemical Research Methods
Ina Varfaj, Michele Protti, Marco Cirrincione, Andrea Carotti, Laura Mercolini, Roccaldo Sardella
Summary: Fentanyl analogues are commonly used in therapy, but the rise in illicitly synthesized fentanyl derivatives has led to hospitalizations and deaths. Chromatographic methods are essential for differentiating enantiomers of fentanyl derivatives. This study successfully separated four fentanyl derivatives' enantiomers under polar-ionic conditions.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Materials Science, Paper & Wood
Geng Li, Xiao Dai, Yixuan Min, Chang Yu, Tomoyuki Ikai, Lili Zhang, Jun Shen, Yoshio Okamoto
Summary: Researchers developed eight organic-inorganic hybrid bead-type chiral packing materials with high organic contents using a modified Stober process in basic conditions. The preparation conditions significantly affected the organic contents and morphology of the hybrid materials, with those having higher organic content showing better enantioseparation ability.
Article
Biochemistry & Molecular Biology
John M. M. Ferraro, Weston J. J. Umstead
Summary: Until recently, chirality has not been a major focus in the study of cannabinoids. However, with the discovery of more cannabinoids, chiral compounds like cannabichromene and cannabicyclol have emerged as potential investigatory candidates. The study aimed to identify analytical separation conditions and adapt them for preparative separation, using column screening on Daicel's immobilized polysaccharide chiral stationary phases. The results showed that CHIRALPAK(R) IK was able to successfully separate all four compounds.
Article
Biochemical Research Methods
Chiara De Luca, Alessandro Buratti, Weston Umstead, Pilar Franco, Alberto Cavazzini, Simona Felletti, Martina Catani
Summary: The increasing popularity and legalization of cannabis products have led to a higher demand for analytical methods to characterize cannabis samples and derivatives. There is a growing interest in studying the potential hazards of uncharacterized minor cannabinoids, including chiral ones, which are not provided by current achiral potency testing methods. Therefore, the development of liquid chromatographic methods for the separation of enantiomers of cannabinoids is of great importance, although more research is needed in this field due to the lack of optically pure standards.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Petra Vankatova, Anna Kubickova, Kveta Kalikova
Summary: A study was conducted on the effects of mobile phase composition and column temperature on enantiomer elution order using chiral rod-like liquid crystalline materials. The results showed that factors such as type and content of cosolvent, as well as temperature, could influence the elution order of enantiomers.
Article
Chemistry, Medicinal
Takafumi Onishi, Takunori Ueda, Kenichi Yoshida, Kosuke Uosaki, Hiroyuki Ando, Ryota Hamasaki, Atsushi Ohnishi
Summary: This study compares the chiral recognition abilities of different immobilized CSPs under normal phase liquid chromatography conditions. It finds that CHIRALPAK IG shows notable chiral recognition abilities and durability, with improved chiral separation selectivities for various racemates.
Review
Chemistry, Medicinal
David Jin Han Seog, Nguyen van Kien, Jae Jeong Ryoo
Summary: This updated minireview focuses on chiral stationary phases (CSPs) based on amino alcohols. The study highlights the use of amino alcohols as starting materials for the preparation of chiral catalysts in asymmetric organic synthesis and CSPs for chiral separations. Important developments and applications of various CSPs, including Pirkle-type CSPs, ligand exchange CSPs, alpha-amino acid-derived amino alcohol CSPs, and symmetric CSPs, are summarized to propose ideas for developing new CSPs with improved performance.
Correction
Environmental Sciences
Timea Pernyeszi, Viktor Farkas, Attila Felinger, Borbala Boros, Imre Dekany
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2018)
Article
Environmental Sciences
Timea Pernyeszi, Viktor Farkas, Attila Felinger, Borbala Boros, Imre Dekany
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2018)
Article
Biochemical Research Methods
Jozsef Simon, Csanad Redei, Attila Felinger
JOURNAL OF CHROMATOGRAPHY A
(2019)
Article
Pharmacology & Pharmacy
Adam Horvath, Balint Botz, Tames Kiss, Kata Cseko, Ibolya Kiss, Attila Felinger, Tamara Szabados, Eva Kenyeres, Peter Bencsik, Attila Mocsai, Peter Ferdinandy, Zsuzsanna Helyes
FRONTIERS IN PHARMACOLOGY
(2019)
Article
Biochemical Research Methods
Iza Matarashvili, Giorgi Kobidze, Aluda Chelidze, Gizo Dolidze, Nino Beridze, Giorgi Jibuti, Tivadar Farkas, Bezhan Chankvetadze
JOURNAL OF CHROMATOGRAPHY A
(2019)
Article
Biochemical Research Methods
Dora Zelenyanszki, Nandor Lambert, Fabrice Gritti, Attila Felinger
JOURNAL OF CHROMATOGRAPHY A
(2019)
Article
Biochemical Research Methods
Csanad Redei, Attila Felinger
JOURNAL OF CHROMATOGRAPHY A
(2019)
Article
Biochemical Research Methods
Dora Zelenyanszki, Adrienn Mester, Attila Felinger
Article
Biochemical Research Methods
Annamaria Sepsey, Eva Horvath, Martina Catani, Attila Felinger
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Biochemical Research Methods
Muhamad Yahia Kazmouz, Csanad Redei, Attila Felinger
Summary: The study determined the surface excess isotherms of methanol on reversed phase stationary phases under two different operational conditions. The separation efficiency and the influence of sample solvents on it were investigated, along with a discussion on surface adsorbent heterogeneity.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Csanad Redei, Attila Felinger
Summary: This study investigates several factors affecting the variation of mass flow-rate in supercritical fluid chromatography (SFC), including different column configurations, experimental conditions, and disturbances introduced by injections. The results show that the mass flow-rate differs when measured at the inlet or the outlet of the column, and different columns exhibit different tendencies of variations.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Denes Szerencses, Annamaria Sepsey, Attila Felinger
Summary: The physical meaning of the calculated enthalpy and entropy values in chromatography is questionable, and the surface heterogeneity in chiral chromatography needs to be reevaluated. Factors such as column length, flow rate, and the instrument used also affect the calculated values.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Dora Zelenyanszki, Attila Felinger
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)