Article
Food Science & Technology
Alberto Pintor-Cora, Angel Alegria, Jose Ramos-Vivas, Maria-Luisa Garcia-Lopez, Jesus A. Santos, Jose M. Rodriguez-Calleja
Summary: This study investigated the presence of antimicrobial-resistant Enterobacter cloacae complex strains in fresh vegetables and their cultivation environments. The findings showed the presence of AmpC-producing ECC isolates, some of which exhibited resistance to the last resort antibiotic colistin. A colistin-resistant E. kobei strain was detected in irrigation water for the first time in Spain, highlighting the transferability of resistance genes. Monitoring and addressing antimicrobial-resistant ECC strains in fresh vegetables and their production environments is important to mitigate potential risks to public health from a One Health perspective.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Microbiology
Shixing Liu, Renchi Fang, Ying Zhang, Lijiang Chen, Na Huang, Kaihang Yu, Cui Zhou, Jianming Cao, Tieli Zhou
Summary: This study found that the resistance mechanisms of carbapenem-resistant and colistin-resistant ECC strains do not overlap, but the presence of ECC strains carrying multiple resistance genes may pose a potential risk for the spread of carbapenem and colistin resistance.
Article
Microbiology
Jiayao Guan, Letian Li, Lin Zheng, Gejin Lu, Ying Wang, Sulaiman Lakoh, Stephen Sevalie, Bowen Jiang, Xue Ji, Yang Sun, Jun Liu, Lingwei Zhu, Xuejun Guo
Summary: This study reports the first identification of mcr-10.1 gene in Africa and the mcr gene in Sierra Leone, along with diverse modular structures of mcr-10.1 loci. The mcr-10.1 gene was carried on an Inc(pA1763-KPC) plasmid from an Enterobacter cloacae isolate. Genetic comparison of 19 mcr-10.1 loci revealed diversification in modular structures through complex transposition and homologous recombination.
MICROBIOLOGY SPECTRUM
(2022)
Article
Multidisciplinary Sciences
William Bennett, Katrin Mende, Wesley R. Campbell, Miriam Beckius, Laveta Stewart, Faraz Shaikh, Azizur Rahman, David R. Tribble, Joseph M. Yabes
Summary: This study evaluated the characteristics, antibiotic utilization, and outcomes of battlefield-related Enterobacter cloacae infections. Patients with E. cloacae infections sustained more blast trauma, required more initial blood products, and had longer durations of antibiotic therapy and hospitalizations compared to patients with non-E. cloacae infections.
Article
Microbiology
Lucile Pantel, Francois Guerin, Marine Serri, Francois Gravey, Jessica Houard, Kelly Maurent, Marie Attwood, Alan Noel, Alasdair MacGowan, Emilie Racine, Vincent Cattoir, Maxime Gualtieri
Summary: This study evaluated the activity of NOSO-502 and colistin against clinical isolates of the Enterobacter cloacae complex (ECC) and investigated the associated resistance mechanisms. The results showed that NOSO-502 hetero-resistance in ECC is induced by the expression regulation of the Ea_01758 efflux pump component, and CST-hetero-resistance is conferred via modification of the lipid A by addition of 4-amino-4-deoxy-L-arabinose controlled by PhoPQ. The study provides insights into the resistance mechanisms of ECC and potential targets for developing effective treatments.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Environmental Sciences
Tingting Xu, Chuqiu Zhang, Yang Ji, Jingjie Song, Yang Liu, Yuqi Guo, Kai Zhou
Summary: This study evaluated the occurrence of a recent reported mcr variant, mcr-10, in hospital sewage water. Three Enterobacter roggenkampii strains with high-level colistin resistance were found to carry mcr-10, which was detected on both self-transmissible plasmids and on the chromosome. The study also identified a new genetic context for the mcr-10 gene and highlighted the importance of continuous monitoring of mcr genes in hospital sewage water for understanding and tackling dissemination.
ENVIRONMENTAL POLLUTION
(2021)
Article
Immunology
Toyotaka Sato, Kazuki Harada, Masaru Usui, Shin-ichi Yokota, Motohiro Horiuchi
Summary: This study investigated the colistin susceptibility of Enterobacterales isolated from companion animals in Japan and found that most E. coli were susceptible to colistin, while a small proportion of Klebsiella spp. and Enterobacter spp. were resistant. Moreover, some colistin-resistant Enterobacter spp. belonged to lineages associated with humans and exhibited resistance to multiple classes of antibiotics.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Hong-hao Huang, Yi-xing Lu, Su-juan Wu, Zhen-bao Ma, Dong-ping Zeng, Zhen-ling Zeng
Summary: Carbapenem- and colistin-resistant Enterobacter strains harboring blaIMI were isolated from intestinal samples and the environment of a duck farm in China. The study demonstrated the diversity of carbapenemases in a duck farm and provided evidence for the transmission of bla(IMI) genes. Whole-genome sequencing and comparative genetic analysis revealed the presence of chromosomal and plasmid bla(IMI) and identified the horizontal transmission of bla(IMI)-carrying plasmids.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Infectious Diseases
Aura Falco, Daniela Guerrero, Isabella Garcia, Adriana Correa, Sandra Rivera, Maria Beatriz Olaya, Carlos Aranaga
Summary: The study aimed to molecularly characterize 28 isolates of the E. cloacae complex resistant to cephalosporins and carbapenems from five hospitals in Cali, Colombia. It found that 43% of the isolates carried the bla(KPC-2) gene variant. High genetic diversity was observed among isolates, with the most common sequence type being ST510.
Article
Microbiology
Chen Wang, Mengyun Yin, Xuefei Zhang, Qinglan Guo, Minggui Wang
Summary: A new quinolone resistance gene qnrE3 was identified, providing decreased susceptibility to fluoroquinolones like qnrE1 and qnrE2. Phylogenetic analysis revealed two major clusters, with qnrE3 and qnrE(Eas2) alleles possibly captured and mobilized between different bacterial strains. The study also suggested E. mori as the probable source of qnrE1 to qnrE3 alleles, while E. asburiae serves as the reservoir for qnrE4.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Hongwei Zhou, Siheng Wang, Yuchen Wu, Ning Dong, Xiaoyang Ju, Chang Cai, Ruichao Li, Yan Li, Congcong Liu, Jiayue Lu, Edward Wai-Chi Chan, Sheng Chen, Rong Zhang, Zhangqi Shen
Summary: This study investigated the prevalence of mcr-9 and mcr-10 in Enterobacter cloacae complex (ECC) strains in China and found that the coexistence and co-transfer of mcr genes and carbapenemase genes pose an urgent threat to public health.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Microbiology
Shixing Liu, Liqiong Chen, Lingbo Wang, Beibei Zhou, Dandan Ye, Xiangkuo Zheng, Yishuai Lin, Weiliang Zeng, Tieli Zhou, Jianzhong Ye
Summary: This study aimed to characterize and explore the differences among species of the Enterobacter cloacae complex (ECC). The results showed that different clusters of ECC had varying resistance, biofilm-forming ability, mobility, and virulence. Clusters IX, VIII, and I were the predominant clusters with high resistance and pathogenicity. Cluster II was a special taxon with a strong biofilm formation ability but low resistance, virulence, and pathogenicity. The findings highlight the need for clinical classification methods to guide the treatment of ECC infections.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Dennis Nurjadi, Kaan Kocer, Quan Chanthalangsy, Sabrina Klein, Klaus Heeg, Sebastien Boutin
Summary: This study found that the emergence of resistance to Cefiderocol in metallo-beta-lactamase-producing bacteria is facilitated by mutations in the CirA siderophore receptor, which are induced by the New Delhi metallo-beta-lactamase. However, inhibiting the metallo-beta-lactamase activity using dipicolinic acid can successfully prevent the development of Cefiderocol-resistant mutants. Therefore, caution should be taken when using Cefiderocol to treat infections caused by metallo-beta-lactamase-producing bacteria.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Yiping Wu, Ruowen He, Mingyang Qin, Yanxian Yang, Jieyun Chen, Yu Feng, Xiaoxue Liang, Wenbin Deng, Xin Ding, Li-Na Qin, Kang Liao, Yongqiang Yang, Guo-Bao Tian
Summary: We identified two tet(X4)-positive E. cloacae isolates and found that the pTECL_2-190k-tetX4-like plasmid has been widely disseminated across various species of Enterobacteriaceae. By analyzing 85 tet(X4)-carrying plasmids, we determined that these plasmids have multiple replicon types, including IncFIA, IncHI1A, and IncHI1B.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Christian Xedzro, Toshi Shimamoto, Liansheng Yu, Hui Zuo, Yo Sugawara, Motoyuki Sugai, Tadashi Shimamoto
Summary: This study reports the emergence of Enterobacter cloacae and Raoultella ornithinolytica carrying the mcr-9.1 gene in vegetables from Japan. The presence of colistin-resistant bacteria in vegetables is a significant public health concern. Monitoring and preventing the dissemination of mcr homologs in the vegetable industry is crucial.
MICROBIOLOGY SPECTRUM
(2023)
Article
Public, Environmental & Occupational Health
Harriet Fisher, Matthew Hickman, Joanne Ferrie, Karen Evans, Michael Bell, Julie Yates, Marion Roderick, Rosy Reynolds, John MacLeod, Suzanne Audrey
Summary: The study found that introducing new consent procedures can improve the uptake of HPV vaccination and overcome the trend of decreasing uptake. However, there was no evidence of a reduction in inequalities.
JOURNAL OF PUBLIC HEALTH
(2022)
Article
Pharmacology & Pharmacy
Niamh K. Martin, Juan Dominguez-Robles, Sarah A. Stewart, Victoria A. Cornelius, Qonita Kurnia Anjani, Emilia Utomo, Inmaculada Garcia-Romero, Ryan F. Donnelly, Andriana Margariti, Dimitrios A. Lamprou, Eneko Larraneta
Summary: This study presents a 3D printing method for developing medicated vascular prostheses using FDM technology, demonstrating antimicrobial properties and sustained release effects of rifampicin-containing TPU filaments. Additionally, dipyridamole-containing TPU filaments were successfully prepared, and vascular grafts containing two drugs were fabricated using a dual extrusion 3D printer.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Public, Environmental & Occupational Health
T. Zhang, C. Robin, S. Cai, C. Sawyer, W. Rice, L. E. Smith, R. Amlot, G. J. Rubin, R. Reynolds, L. Yardley, M. Hickman, I Oliver, H. Lambert
Summary: This study evaluated the impact and effectiveness of public health information provided to passengers arriving at major UK ports during the early stages of the COVID-19 outbreak. Most surveyed passengers correctly identified symptoms, actions to take, and found the advice understandable and trustworthy. Passengers acquired knowledge from various sources and saw the provision of information alone as an insufficient official response.
Article
Infectious Diseases
Carolyne Horner, Shazad Mushtaq, Michael Allen, Christopher Longshaw, Rosy Reynolds, David M. Livermore
Summary: This study compared resistance prevalence between bloodstream infection and lower respiratory tract infection isolates, finding that resistance was more prevalent in LRTI isolates, especially for bacteria like Pseudomonas aeruginosa, Streptococcus pneumoniae, and Staphylococcus aureus.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Florence Tydeman, Noel Craine, Kimberley Kavanagh, Helen Adams, Rosy Reynolds, Victoria McClure, Harriet Hughes, Matt Hickman, Chris Robertson
Summary: This study found that total antibiotic prescribing by general practitioners was associated with an increased risk of Clostridioides difficile infection (CDI) incidence, particularly clindamycin prescribing showed a positive association with total CDI incidence. Age over 65 and diabetes were also associated with an increased risk of CDI.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Medicine, General & Internal
Harriet Fisher, Karen Evans, Rosy Reynolds, Julie Yates, Marion Roderick, Jo Ferrie, John Macleod, Matthew Hickman, Suzanne Audrey
Summary: Implementing a new policy to provide additional opportunities for consent in the school-based HPV vaccination program can effectively reduce the percentage of unvaccinated young women belonging to families less likely to respond to paper-based methods of gaining consent and at greater risk of developing cervical cancer.
Article
Immunology
Aaron G. Lim, Adam Trickey, Laura H. Thompson, Faran Emmanuel, Tahira E. Reza, Rosy Reynolds, Francois Cholette, Dessalegn Y. Melesse, Chris Archibald, Paul Sandstrom, James F. Blanchard, Peter Vickerman
Summary: This study found that the key risk factors contributing to the explosive HIV epidemic among people who inject drugs in Pakistan include the use of professional injectors, heroin use, and frequent injecting. By reducing the prevalence of these risk factors, overall HIV incidence can be significantly decreased.
OPEN FORUM INFECTIOUS DISEASES
(2021)
Article
Cell Biology
Thomas Delerue, Vivek Anantharaman, Michael C. Gilmore, David L. Popham, Felipe Cava, L. Aravind, Kumaran S. Ramamurthi
Summary: The protein SpoVID plays a crucial role in monitoring the polymerization state of the coat basement layer in Bacillus subtilis spores, halting sporulation when coat assembly is initiated improperly. By interacting with the membrane-bound lipid II peptidoglycan precursor, SpoVID prevents cortex assembly, linking the roles of coat and cortex assembly together.
DEVELOPMENTAL CELL
(2022)
Letter
Infectious Diseases
Rosy Reynolds, Shazad Mushtaq, Michael K. Allen, Carolyne Horner, Christopher Longshaw, David M. Livermore
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Medicine, General & Internal
Padraig Dixon, William Hollingworth, Katie Pike, Rosy Reynolds, Margaret Stoddart, Alasdair MacGowan
Summary: This study assessed the cost-effectiveness of rapid microbial identification by MALDI-TOF mass spectrometry for bloodstream infections. The results indicate that adjunctive MALDI-TOF diagnosis may not be cost-effective when measured as cost per death avoided at 28 days.
Article
Microbiology
Helena Chan, Najwa Taib, Michael C. Gilmore, Ahmed M. T. Mohamed, Kieran Hanna, Johana Luhur, Hieu Nguyen, Elham Hafiz, Felipe Cava, Simonetta Gribaldo, David Rudner, Christopher D. A. Rodrigues
Summary: Cell envelope remodeling is critical for bacteria growth and division, especially during the development of highly resistant endospores. This study identifies two new factors, YrvJ and MurAB, that are required for efficient envelope remodeling during sporulation, highlighting the importance of peptidoglycan precursor synthesis for engulfment efficiency in B. subtilis and likely other endospore-forming bacteria.
Article
Public, Environmental & Occupational Health
Gemma Lasseter, Polly Compston, Charlotte Robin, Helen Lambert, Matthew Hickman, Sarah Denford, Rosy Reynolds, Juan Zhang, Shenghan Cai, Tingting Zhang, Louise E. Smith, G. James Rubin, Lucy Yardley, Richard Amlot, Isabel Oliver
Summary: This study aimed to explore the impact and responses of shielding on the health and wellbeing of clinically extremely vulnerable (CEV) individuals in Southwest England during the first COVID-19 lockdown. The survey and interviews revealed that 'official' communication from NHS England or General Practitioner (GP) marked the start of shielding for participants. Worry about COVID-19 was common and shielding negatively affected both physical and mental health for some respondents.
Article
Multidisciplinary Sciences
Michael C. Gilmore, Felipe Cava
Summary: This study reveals the presence of an alternative transporter, YejBEF-YepA, in the plant pathogen Agrobacterium tumefaciens, which plays a key role in the uptake and recycling of cell wall fragments. This transporter is crucial for cell wall integrity and contributes to beta-lactam resistance in A. tumefaciens.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Brent W. Simpson, Michael C. Gilmore, Amanda Briann Mclean, Felipe Cava, M. Stephen Trent
Summary: Bacteria produce a layer of peptidoglycan to protect and maintain cell shape, and as they grow and divide, this layer is remodeled and fragments are released. Escherichia coli utilizes different strategies to internalize and reuse these fragments, with two transporters, AmpG and MppA/Opp, playing key roles. The regulation and function of these transporters are influenced by carbon source and growth phase, and their scavenging capabilities contribute to the recycling of peptidoglycan fragments.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Infectious Diseases
Carolyne Horner, Shazad Mushtaq, Michael Allen, Russell Hope, Sarah Gerver, Christopher Longshaw, Rosy Reynolds, Neil Woodford, David M. Livermore
Summary: The review of two UK bacteraemia surveillance systems revealed a rising proportion of E. faecium in enterococcal bloodstream infections and stable resistance trends, particularly E. faecium's resistance to antibiotics. This highlights the importance of monitoring and addressing the increasing prevalence of multiresistant E. faecium strains in clinical settings.
JAC-ANTIMICROBIAL RESISTANCE
(2021)