Article
Nanoscience & Nanotechnology
Maximilian A. Beach, Serena L. Y. Teo, Moore Z. Chen, Samuel A. Smith, Colin W. Pouton, Angus P. R. Johnston, Georgina K. Such
Summary: Nanoparticles have the potential to revolutionize drug delivery, but overcoming endosomal escape remains a significant challenge. Recent research focuses on designing nanoparticles that can escape endosomes in response to biological stimuli such as pH changes, which can improve drug delivery efficiency.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Xun Zhang, Zhizhi Fan, Qinghua Wang, Xiangbo Kong, Fu Liu, Jiaxing Fang, Sufang Zhang, Zhen Zhang
Summary: RNA interference (RNAi) technology is a promising method for pest control, but its effectiveness varies among different insect species. This study focused on the fall webworm and found that the efficiency of RNAi through dsRNA injection was relatively low, primarily due to dsRNA degradation by dsRNase enzymes. The study also demonstrated that silencing HcdsRNase genes can increase the effectiveness of RNAi.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Zhongzheng Ma, Yang Zheng, Zijian Chao, Hongtao Chen, Yunhui Zhang, Meizhen Yin, Jie Shen, Shuo Yan
Summary: A star polymer with positively-charged tertiary amine was synthesized and used to investigate the mechanism of gene delivery. The star polymer could protect gene fragments from degradation and promote cellular uptake and endocytosis. Transcriptome analysis revealed up-regulation of key genes involved in endocytosis. This study provides the first direct visualization of the detailed cellular process and mechanism of nanocarrier-mediated gene delivery.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mohammad Vatanparast, Majid Kazzazi, Seyedeh Minoo Sajjadian, Youngjin Park
Summary: The study found that Ha-CHY is a new candidate for developing an effective dsRNA-based pesticide for controlling Helicoverpa armigera. In addition, sonicated bacterial cells showed higher RNA interference efficiency when orally administered.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Entomology
Clauvis Nji Tizi Taning, Shunhua Gui, Kristof De Schutter, Mona Jahani, Nathaly Lara Castellanos, Olivier Christiaens, Guy Smagghe
Summary: This study confirmed the susceptibility of bumblebees to ingested dsRNA targeting specific genes, and found no significant off-target effects on gene expression in bumblebees following exposure to a fusion dsRNA product designed for pest control.
JOURNAL OF PEST SCIENCE
(2021)
Article
Fisheries
Feibiao Song, Lanmei Wang, Zhenzhen Yu, Wenbin Zhu, Jianjun Fu, Zaijie Dong
Summary: RNA interference is an important method in studying the gene function of organisms. In this study, the function of igf3 in gonadal development of teleost fish was researched by conducting long-term igf3 interference experiments. The findings indicate that igf3 knockdown may have a negative effect on gonadal development of C. carpio through the HPG axis.
AQUACULTURE RESEARCH
(2021)
Article
Fisheries
Sarah K. W. Au, Iliana V. Portelli, Stephanie J. DeWitte-Orr
Summary: This study provides evidence in fish that sequence specific long dsRNA induces potent gene expression silencing and antiviral responses in vitro via an RNAi-like mechanism instead of an IFN-dependent response.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Jungsoo Park, Michael Evangelopoulos, Matthew Kuo Vasher, Sergej Kudruk, Namrata Ramani, Vinzenz Mayer, Alexander Carlos Solivan, Andrew Lee, Chad Alexander Mirkin
Summary: Formulating small interfering RNAs (siRNAs) as spherical nucleic acids (SNAs) enhances their stability, cellular uptake, and gene silencing activity. The use of calcium chloride (CaCl2) instead of sodium chloride (NaCl) increases cytosolic delivery and reduces endosomal entrapment, resulting in improved efficacy.
Review
Plant Sciences
Rahul B. Nitnavare, Joorie Bhattacharya, Satnam Singh, Amardeep Kour, Malcolm J. Hawkesford, Naveen Arora
Summary: RNA interference is a method of gene silencing that has potential applications in pest management in various insect classes. Different insect classes exhibit varying levels of response to RNAi, with coleopterans showing the highest efficiency, hemipterans being relatively inefficient, and lepidopterans showing the least response. Various methods have been developed to safely deliver dsRNA into insect biological systems.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Bao Tram L. Hoang, Stephen J. Fletcher, Christopher A. Brosnan, Amol B. Ghodke, Narelle Manzie, Neena Mitter
Summary: RNA interference (RNAi) is a powerful tool utilized for crop protection, with spray-induced gene silencing (SIGS) being a non-transgenic approach that relies on double-stranded RNA (dsRNA) for inducing RNAi. However, challenges in efficiency arise in plants and insects, where mechanisms of dsRNA uptake and movement through plant tissues need to be thoroughly investigated for effective delivery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Gadigavarahalli Subbareddy Uma, Manjesh Saakre, Jyoti Singh, Vinay K. Kalia
Summary: The silencing of the Agsuc1 gene effectively reduced the population of cotton aphids and decreased the number of offspring, leading to significant reduction in crop damage. Bacterial expression for dsRNA production is a cost-effective method and has the potential to be used as a biopesticide.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
Article
Forestry
Minghui Zhang, Xiaoqian Weng, Qing Li, Liangjing Sheng, Yajie Guo, Liya Xiong, Feiping Zhang, Songqing Wu
Summary: Monochamus alternatus Hope (Coleoptera: Cerambycidae), a serious pest of Pinus massoniana in many Asian countries, has caused billions of dollars in economic losses. In this study, the eye pigment gene of M. alternatus was clarified, and its knockdown phenotype was investigated. Two homologous genes, tryptophan 2,3-dioxygenase (TDO) and kynurenine-3-monooxygenase (KMO), were found to be expressed in various developmental stages of M. alternatus. Knockdown experiments using RNA interference technology showed that the expressions of MaTDO and MaKMO were significantly inhibited, resulting in a change in eye color without affecting the beetles' activity. Therefore, MaTDO and MaKMO can be used as tag genes for M. alternatus, and a dominant marker system based on eye color can be developed for the control of this invasive beetle.
Article
Biochemistry & Molecular Biology
Henry Kolge, Kartiki Kadam, Vandana Ghormade
Summary: This study demonstrates the effectiveness of ionotropically synthesized cationic chitosan nanoparticles (CNPs) in delivering double stranded RNA (dsRNA) for gene silencing in resistant insect pests, such as Helicoverpa armigera. The CNPs efficiently loaded dsRNA and protected it from degradation in the insect gut. The CNPs:dsRNA complex effectively silenced target genes and suppressed enzyme activities, resulting in reduced pupation and impaired moth emergence. Importantly, the specific dsRNA did not harm non-target insects, making the CNPs:dsRNA complex a safe and targeted insecticide for sustainable crop protection.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Physiology
Thomas-Wolf Verdonckt, Jozef Vanden Broeck
Summary: RNA interference (RNAi) is a crucial pathway for regulating gene expression, and this paper presents a method for cost-effective and large-scale production of high-quality dsRNA from the HT115 bacterial system. The applicability of bacteria-derived dsRNA for in vitro experiments is also explored.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Mathematics
Sergei Fedotov, Dmitri Alexandrov, Ilya Starodumov, Nickolay Korabel
Summary: In this paper, a stochastic model is established to study the dynamics of active Rab5 and Rab7 proteins on the surface of endosomes and the acidification process affecting the escape of viruses and pH-responsive nanoparticles.
Article
Entomology
Xien Chen, Subba Reddy Palli
Summary: A piggyBac-based germline transformation system was established in the fall armyworm, Spodoptera frugiperda, a global agricultural pest. Tissue-specific promoters were identified and used to drive the expression of transgenes, resulting in tissue-specific overexpression. Overexpression of certain genes in the midgut was found to confer insecticide resistance.
JOURNAL OF PEST SCIENCE
(2023)
Article
Entomology
Xien Chen, Subba Reddy Palli
Summary: This study reports the establishment of multiple transgenic CRISPR/Cas9-based genome editing methods in the global agricultural pest, fall armyworm, Spodoptera frugiperda. Fluorescent proteins suitable for screening for transgenic fall armyworm were identified. The use of U6-tRNA-sgRNA system for sgRNA expression was found to be more efficient in inducing target gene knockout. These methods provide valuable tools for gene editing and functional genomics studies in the fall armyworm and other lepidopteran pests.
JOURNAL OF PEST SCIENCE
(2023)
Editorial Material
Agronomy
Yulin Gao, Andrei Alyokhin, Ralf Nauen, Raul N. C. Guedes, Subba Reddy Palli
PEST MANAGEMENT SCIENCE
(2022)
Article
Genetics & Heredity
Guang-Hua Luo, Xi-En Chen, Yao-Yu Jiao, Guan-Heng Zhu, Ru Zhang, Ramesh Kumar Dhandapani, Ji-Chao Fang, Subba Reddy Palli
Summary: This study reveals that the SoxC gene plays a crucial role in the regulation of insect ecdysis, and is associated with the expression of neuropeptide genes such as EH.
FRONTIERS IN GENETICS
(2022)
Review
Entomology
Marc Kenis, Giovanni Benelli, Antonio Biondi, Paul-Andre Calatayud, Roger Day, Nicolas Desneux, Rhett D. Harrison, Darren Kriticos, Ivan Rwomushana, Johnnie van den Berg, Francois Verheggen, Yong-Jun Zhang, Lakpo Koku Agboyi, Regis Besmer Ahissou, Malick N. Ba, Julio Bernal, Adeney de Freitas Bueno, Yves Carriere, Geraldo Andrade Carvalho, Xue-Xin Chen, Lizette Cicero, Hannalene du Plessis, Regan Early, Patrick Fallet, Komi K. M. Fiaboe, Dnyaneshwar M. Firake, Georg Goergen, Astrid T. Groot, Raul N. C. Guedes, Ankita Gupta, Gao Hu, Fn Huang, Lara R. Jaber, Edi A. Malo, Christina B. McCarthy, Robert L. Meagher, Samira Mohamed, David Mota Sanchez, Rodney N. Nagoshi, Nicolas Negre, Saliou Niassy, Noboru Ota, Casper Nyamukondiwa, Celso Omoto, Subba Reddy Palli, Roman Pavela, Ricardo Ramirez-Romero, Julio C. Rojas, Sevgan Subramanian, Bruce E. Tabashnik, Wee Tek Tay, Eduardo Gabriel Virla, Su Wang, Trevor Williams, Lian-Sheng Zang, Lisheng Zhang, Kongming Wu
Summary: This article provides a comprehensive review of the fall armyworm, including its biology, worldwide status and spread, management strategies, and recommendations for sustainable management in invaded regions.
ENTOMOLOGIA GENERALIS
(2023)
Article
Agronomy
Xingeng Wang, Jessica Faucher, Ramesh Kumar Dhandapani, Jian J. Duan, Subba Reddy Palli
Summary: This study investigated the impact of dsRNA-based insecticides on the survival and behavior of a parasitoid wasp. The results showed that direct injection of dsRNA did not affect the wasp's survival, but it indirectly affected the suitability and detectability of host larvae.
PEST MANAGEMENT SCIENCE
(2023)
Article
Infectious Diseases
Anna R. Pasternak, Subba R. Palli
Summary: Kentucky experiences a high incidence of ehrlichiosis, primarily transmitted by the lone star tick. Surveillance of tick distribution and pathogen detection is crucial for monitoring the expansion of this important tick vector and the increase in tick-borne diseases.
TICKS AND TICK-BORNE DISEASES
(2023)
Article
Engineering, Environmental
Lin Liu, Olga V. Tsyusko, Jason M. Unrine, Shuang Liu, Yidan Liu, Lulu Guo, Gehong Wei, Chun Chen
Summary: The effects of zinc oxide nanoparticles and sulfidized zinc oxide nanoparticles on the carbon cycling in the legume rhizosphere were investigated. It was found that these nanoparticles significantly increased the dissolved organic carbon concentrations and stimulated the growth of microbes involved in organic matter degradation. The results provide new insights into the ecological functions of nanoparticles in soil-plant systems.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anye Wamucho, Jason Unrine, John May, Olga Tsyusko
Summary: Previous studies have demonstrated multigenerational and transgenerational reproductive toxicity in Caenorhabditis elegans after exposure to silver nanoparticles (Ag-NPs) and silver ions (AgNO3). However, the mechanism of transfer of increased reproductive sensitivity to subsequent generations is not well understood. This study investigates the changes in DNA methylation at the epigenetic mark N6-methyl-2'-deoxyadenosine (6mdA) after multigenerational exposure of C. elegans to pristine and sulfidized Ag-NPs and AgNO3. The results suggest the involvement of an epigenetic mark in AgNO3 reproductive toxicity and indicate potential differences in toxicity mechanisms between AgNO3 and Ag-NPs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Heng Jiang, Xiangkun Meng, Nan Zhang, Huichen Ge, Jiaping Wei, Kun Qian, Yang Zheng, Yoonseong Park, Subba Reddy Palli, Jianjun Wang
Summary: This study found that deltamethrin-induced oxidative stress activates AMPK, promoting the nuclear translocation of CncC through phosphorylation. CncC acts as a transcription activator of detoxification genes and regulates the expression of genes involved in ecdysteroid biosynthesis and juvenile hormone degradation. This results in increased ecdysteroid levels, decreased juvenile hormone titer, and down-regulated vitellogenin gene expression.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Jinmo Koo, Dhandapani Gurusamy, Subba Reddy Palli
Summary: There is limited success in using exogenous small interfering RNA (siRNA) or small hairpin RNA (shRNA) to trigger RNA interference (RNAi) in insects. Instead, long double-stranded RNA (dsRNA) is used to induce gene knockdown in insects. In this study, the potency of si/sh RNAs and dsRNA was compared in Colorado potato beetle (CPB) cells. CPB cells showed a highly efficient RNAi response to dsRNA, but si/sh RNAs were inefficient in triggering RNAi in CPB cells.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Environmental Sciences
Zeinah Elhaj Baddar, Xiaoyu Xu, Breann Spencer
Summary: The study used DGTs and peepers to investigate the spatial and seasonal effects on metal bioavailability in constructed wetlands. The results showed similar seasonal trends in metal concentrations, with lower levels in warm months and higher levels in cool months. The sediments acted as a sink for Cu and Zn, with a low metal resupply capacity.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2023)
Article
Chemistry, Inorganic & Nuclear
Richard J. Mitchell, Anitha S. Gowda, Alexander G. Olivelli, Aron J. Huckaba, Sean Parkin, Jason M. Unrine, Viral Oza, Jessica S. Blackburn, Folami Ladipo, David K. Heidary, Edith C. Glazer
Summary: Cancer cells rely on glycolysis for energy but recent studies have shown that their mitochondria still play an active role. The study synthesized and characterized ruthenium compounds with triarylphosphine-containing bipyridyl ligands which showed high depolarizing capabilities on mitochondrial membranes in cancer cells. These compounds have the potential for anticancer applications due to their potency against cancer cells and low toxicity in zebrafish embryos.
INORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Yaoyu Jiao, Subba Reddy Palli
Summary: m6A methylation is a common modification of mRNA, which regulates multiple stages of mRNA processing. In this study, the role of m6A modification in insect development was explored using the red flour beetle as a model. Knockdown of m6A writers and readers disrupted ecdysis during eclosion, sterilized the insects, and affected egg laying and embryonic development.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Richard J. J. Mitchell, Sarah M. M. Kriger, Alexander D. D. Fenton, Dmytro Havrylyuk, Ankit Pandeya, Yang Sun, Tami Smith, Jason E. E. DeRouchey, Jason M. M. Unrine, Viral Oza, Jessica S. S. Blackburn, Yinan Wei, David K. K. Heidary, Edith C. C. Glazer
Summary: A potent Ru(ii) agent with cytotoxicity in cancer cells and minimal toxicity in zebrafish embryos has been designed as a monofunctional anticancer agent, mimicking the biological effects of phenanthriplatin.
RSC CHEMICAL BIOLOGY
(2023)
