Article
Biochemistry & Molecular Biology
Daehwan Choi, Takenobu Ishii, Munetada Ishikawa, Tomohisa Ootake, Hirokazu Kamei, Kohei Nagai, Kenji Sueishi
Summary: This study investigated the impact of osteoblast vibration on cell cycle, differentiation, and aging. Primary maxilla osteoblasts were subjected to vibration at different frequencies, and the results showed that cell proliferation increased significantly at 30 and 300 Hz. Differentiation of osteoblasts was also enhanced at 30 Hz, while markers of aging were activated. Overall, providing optimal vibration to osteoblasts promotes cell cycle progression and differentiation, but also induces cell aging.
Article
Multidisciplinary Sciences
Marco D'Ario, Rafael Tavares, Katharina Schiessl, Benedicte Desvoyes, Crisanto Gutierrez, Martin Howard, Robert Sablowski
Summary: The study reveals that cell size variability in the Arabidopsis shoot stem cell niche is corrected by adjusting the growth period before DNA synthesis. KRP4 and FBL17 proteins play key roles in removing excess cell cycle regulators, ensuring daughter cells are born with comparable amounts of KRP4. This suggests a potential mechanism where a cell cycle regulator associated with mitotic chromosomes can read DNA content as a cell size-independent scale.
Article
Biochemistry & Molecular Biology
Seung-Jun Lee, Jinah Park, Dong-Joon Lee, Keishi Otsu, Pyunggang Kim, Seiya Mizuno, Min-Jung Lee, Hyun-Yi Kim, Hidemitsu Harada, Satoru Takahashi, Seong-Jin Kim, Han-Sung Jung
Summary: This study investigated the role of MAST4 in spermatogenesis in mice, revealing its importance in the localization to Sertoli cells, phosphorylation of ERM, and maintenance of SSC self-renewal.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Biology
Martin Jinye Zhang, Angela Oliveira Pisco, Spyros Darmanis, James Zou
Summary: The study systematically characterized gene expression changes during aging across diverse cell types, revealing that shared aging-related genes exhibit coordinated expression changes in multiple tissue-cell types, suggesting a coordinated global aging behavior at the organismal level. Additionally, age-related expression changes specific to each functional category of tissue-cell types were identified, providing one of the most comprehensive and systematic characterizations of the molecular signatures of aging in a mammalian system.
Article
Multidisciplinary Sciences
Alexander Mattebo, Taha Sen, Maria Jassinskaja, Kristyna Pimkova, Isabel Prieto Gonzalez-Albo, Abdul Ghani Alattar, Ramprasad Ramakrishnan, Stefan Lang, Marcus Jaras, Jenny Hansson, Shamit Soneji, Sofie Singbrant, Emile van den Akker, Johan Flygare
Summary: YPEL family genes are highly conserved and potentially involved in essential cellular processes. Ypel4, specifically expressed in late terminal erythroid differentiation, plays a role in maintaining normal red cell membrane integrity. Ypel4-null mice exhibit secondary polycythemia and intrinsic defects in red blood cells.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Meghali Aich, Asgar Hussain Ansari, Li Ding, Vytautas Iesmantavicius, Deepanjan Paul, Chunaram Choudhary, Souvik Maiti, Frank Buchholz, Debojyoti Chakraborty
Summary: This study reveals that the lncRNA-associated protein TOBF1 regulates the alternative splicing of transcripts necessary for maintaining stem cell identity in mouse ESCs. One of the affected genes is serine/arginine splicing factor 1 (SRSF1), whose splicing leads to global changes in splicing and expression of downstream genes involved in the maintenance of ESC pluripotency. The study shows that TOBF1 chromatin occupancy, pluripotency-associated OCT-SOX binding motifs, and transcripts undergoing differential expression and splicing converge in specific nuclear territories, collectively contributing to the maintenance of mouse ESC identity.
Article
Multidisciplinary Sciences
Weimin Zhang, Ran Brosh, Laura H. McCulloch, Yinan Zhu, Hannah Ashe, Gwen Ellis, Brendan R. Camellato, Sang Yong Kim, Matthew T. Maurano, Jef D. Boeke
Summary: This study presents a new conditional knockout strategy that enables efficient counter selection in mouse embryonic stem cells. The strategy overwrites counter selectable markers and allows the removal or replacement of wild-type DNA with the desired payload DNA. The restored native gene function in the conditional knockout cells facilitates subsequent mouse development and the generality of the strategy is demonstrated through the knockout of other genes.
Article
Biochemistry & Molecular Biology
Perry C. Caviness, Dongzheng Gai, Oxana P. Lazarenko, Michael L. Blackburn, Fenghuang Zhan, Jin-Ran Chen
Summary: Osteoclasts derived from hematopoietic stem cells play a crucial role in bone resorption. Identifying new molecules that can epigenetically regulate osteoclastogenesis is essential for the development of novel treatments for bone deterioration disorders like osteoporosis. The protein Ezh2 has been found to be associated with the epigenetic regulation of various cellular processes, but its role in bone cell development is still not fully understood.
Article
Cardiac & Cardiovascular Systems
Aleksandra Boikova, Megan J. Bywater, Gregory A. Quaife-Ryan, Jasmin Straube, Lucy Thompson, Camilla Ascanelli, Trevor D. Littlewood, Gerard I. Evan, James E. Hudson, Catherine H. Wilson
Summary: The study reveals that Myc and HRas can cooperate to induce proliferation in adult mammalian cardiomyocytes, but the activity of HRas also leads to cell death, limiting its potential as a regenerative therapeutic target.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Taro Chaya, Yamato Maeda, Ryo Sugimura, Daisuke Okuzaki, Satoshi Watanabe, Leah R. Varner, Daisuke Motooka, Daichi Gyoten, Haruka Yamamoto, Hidemasa Kato, Takahisa Furukawa
Summary: This study found that miR-124a plays an important role in neuronal maturation rather than neurogenesis.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Oncology
Ming-Min Chang, Han-Yu Weng, Meng-Shao Lai, Li-Wen Wang, Shang-Hsun Yang, Chia-Ching Wu, Chia-Yih Wang, Bu-Miin Huang
Summary: This study found that FGF9 can promote cell proliferation and tumorigenesis in TM3 cells through multiple signaling pathways.
AMERICAN JOURNAL OF CANCER RESEARCH
(2022)
Article
Endocrinology & Metabolism
Mengxue Jiang, Zhijian Kuang, Yaohui He, Yin Cao, Tingyan Yu, Jidong Cheng, Wen Liu, Wei Wang
Summary: This study reveals that SNAPIN plays a crucial role in regulating insulin secretion and beta cell proliferation, with its expression influenced by age and diabetes. The function of SNAPIN is achieved through its impact on cell cycle regulation, suggesting it might be a potential pharmacotherapeutic target for diabetes mellitus.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Biochemical Research Methods
Parul Singh, Syed Azmal Ali, Sudarshan Kumar, Ashok Kumar Mohanty
Summary: This study investigates the effects of S100A8 knockout on mammary epithelial cells and reveals that it leads to increased cell proliferation and migration, reduced cell-cell adhesion, and increased apoptosis. Proteomics analysis suggests that S100A8 affects cell cycle progression, proliferation, and survival through the PI3K-Akt pathway. The study emphasizes the importance of maintaining S100A8 expression for healthy cell physiology.
JOURNAL OF PROTEOMICS
(2023)
Article
Cell Biology
Hanna Hueneke, Marion Langeheine, Kristina Rode, Klaus Jung, Adrian Pilatz, Daniela Fietz, Sabine Kliesch, Ralph Brehm
Summary: Adult male Sertoli cell-specific Connexin43 knockout mice (SCCx43KO) exhibit higher Sertoli cell (SC) numbers per seminiferous tubule compared to their wild type (WT) littermates. This study investigated postnatal SC development and found that SCCx43KO mice showed delayed initiation of cell-cycle-inhibitor p27Kip1 synthesis and prolonged synthesis of phosphorylated pRb and Ki67, indicating a prolonged period of SC proliferation. These findings suggest that deletion of Connexin43 may prevent normal SC maturation and alter the proliferation potential of adult SC.
Article
Multidisciplinary Sciences
Danielle C. Croucher, Laura M. Richards, Serges P. Tsofack, Daniel Waller, Zhihua Li, Ellen Nong Wei, Xian Fang Huang, Marta Chesi, P. Leif Bergsagel, Michael Sebag, Trevor J. Pugh, Suzanne Trudel
Summary: The authors used a mouse model of multiple myeloma and single-cell RNA-seq to identify subclonal expression programs that arise during progression, providing insights for targeted therapeutic strategies. This study reveals intratumoural heterogeneity driven by transcriptional variability during active disease and highlights the importance of single-cell analyses in understanding the biological mechanisms of disease progression.
NATURE COMMUNICATIONS
(2021)
Article
Oncology
Kai Wang, Yun Chen, Zhihong Zhao, Meiying Feng, Shouquan Zhang
MOLECULAR MEDICINE REPORTS
(2019)
Article
Reproductive Biology
Yun Chen, Kai Wang, Delong Zhang, Zhihong Zhao, Jianhao Huang, Lele Zhou, Meiying Feng, Junsong Shi, Hengxi Wei, Li Li, Zhenfang Wu, Shouquan Zhang
Article
Veterinary Sciences
Fenglei Gao, Ping Wang, Kai Wang, Yushan Fan, Yuming Chen, Yun Chen, Chao Ye, Meiying Feng, Li Li, Shouquan Zhang, Hengxi Wei
Summary: The study found that CRISP2 protein is specifically expressed in the testis and sperm of adult boars, and is associated with sperm fertilizing ability and boar fertility. Further mechanistic studies are needed to fully understand the role of CRISP2 in boar reproduction.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Endocrinology & Metabolism
Meiying Feng, Kai Wang, Hengxi Wei, Shouquan Zhang, Yun Chen
Summary: Obesity leads to reduced 25OHD(3) concentration and low fertility, while supplementing with 25OHD(3) can increase serum concentration, testosterone level, and sperm motility, but does not improve obesity, liver damage, and CYP2R1 expression. There is a metabolic interference between 25OHD(3) and testosterone in obese individuals with low sperm motility.
Review
Agriculture, Dairy & Animal Science
Kai Wang, Dan Shen, Pengyuan Dai, Chunmei Li
Summary: Particulate matter (PM) is a significant environmental stressor for the poultry industry worldwide, as it can adsorb and carry various pollutants, including pathogens. High concentrations of PM can induce respiratory inflammation and diseases in poultry. However, the exact pathogenic mechanism of PM in poultry houses is still unclear due to its complexity and lack of accurate assays.
Article
Agriculture, Dairy & Animal Science
Kai Wang, Junze Liu, Liangyu Zhao, Yansen Li, Chunmei Li
Summary: The ratio of valine-to-lysine in the diet affects the placental development and reproductive performance of sows. Adjusting the ratio can improve sow performance and increase production efficiency.
ANIMAL PRODUCTION SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Yun Chen, Kai Wang, Shouquan Zhang
Summary: This study used reliable experimental materials and methods to detect the effects of osteopontin (OPN) on boar sperm in vitro. The results showed that OPN significantly improved sperm motility, acrosome reaction ability, and embryo division.
JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Kai Wang, Yun Chen, DeLong Zhang, RongGen Wang, ZhiHong Zhao, MeiYing Feng, HengXi Wei, Li Li, Shouquan Zhang
ANIMAL SCIENCE JOURNAL
(2020)