Article
Biochemical Research Methods
Sejal Davla, Edward Daly, Jenn Nedow, Ari Gritsas, Laura Curran, Lorne Taylor, Donald J. van Meyel
Summary: The study developed a method using LC-MS/MS to quantify six distinct monoamines in extracts from dissected Drosophila and mouse brain tissues. This quantitative LC-MS/MS methodology has important use for simultaneous measurement of distinct neuroactive monoamines from precious biological specimens.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Raphael Goutaudier, Fanny Joly, David Mallet, Magali Bartolomucci, Denis Guicherd, Carole Carcenac, Frederique Vossier, Thibault Dufourd, Sabrina Boulet, Colin Deransart, Benoit Chovelon, Sebastien Carnicella
Summary: This study provides evidence for the involvement of reduced dopamine levels in the dorsolateral striatum in compulsive alcohol use. The researchers showed that selective hypodopaminergia in this pathway leads to compulsive-like alcohol self-administration and alcohol withdrawal-like motivational impairments. Treatment with the monoamine stabilizer OSU6162 transiently decreased compulsive-like alcohol self-administration in vulnerable rats.
MOLECULAR PSYCHIATRY
(2023)
Article
Biochemical Research Methods
Yangzom Dawa, Yurong Du, Qi Wang, Chengbiao Chen, Denglang Zou, Desheng Qi, Jianbin Ma, Jun Dang
Summary: Traditional Tibetan medicine (TTM) provides valuable novel therapeutic lead molecules from natural products. This study successfully separated and identified 4 main radical scavenging compounds from the methanolic extract of Saxifraga atrata using efficient methods. The developed methodology is effective for targeted isolation of high-purity compounds and rapid identification and isolation of DPPH inhibitors from various natural products.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Multidisciplinary Sciences
Chuanzhen Zhao, Kevin M. Cheung, I-Wen Huang, Hongyan Yang, Nako Nakatsuka, Wenfei Liu, Yan Cao, Tianxing Man, Paul S. Weiss, Harold G. Monbouquette, Anne M. Andrews
Summary: Implantable aptamer-field-effect transistor (FET) neuroprobes were developed for monitoring neurotransmitters, enabling femtomolar serotonin detection limits in brain tissue. This study opens opportunities for integrated neural activity recordings at high spatiotemporal resolution by combining these aptamer-FET sensors with other types of Si-based implantable probes.
Article
Biochemical Research Methods
Christopher S. Skaggs, Brian A. Logue
Summary: A method for ultratrace determination of atrazine in soil using ICECLES and HPLC-MS/MS was developed to address difficulties in atrazine analysis in soil. This method showed a wide linear range, excellent limits of quantification and detection, as well as good accuracy and precision. Testing on field soil samples confirmed the ability of the method to provide ultratrace detection of atrazine.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Xiao-wei Zhang, Yu-ting Chen, Xue Feng, Lu-ya Li, Ke-wei Song, Yu-peng Sun, Guo-hua Zhang, Lan-tong Zhang
Summary: In this study, the metabolism of celastrol in vivo and in vitro was comprehensively characterized using ultra-high-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry. A total of 43 metabolites were identified and characterized, and it was found that the biotransformation capacity of the intestinal tract exceeded that of the liver. These findings provide a basis for understanding the pharmacological mechanisms of celastrol and have potential implications for clinical applications.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Physical
Yushen Jin, Yan Qi, Chu Tang, Bing Shao
Summary: The research demonstrates that by controlling the number of MOF layers on the surface of Fe3O4 nanoparticles, efficient removal of phytochromes in vegetables and satisfactory recoveries can be achieved. The nanospheres show preferable stabilities in both organic solvents and water with excellent reusability. This research suggests that versatile nanospheres may bring opportunities to the next generation of sample pretreatment materials for the non-targeted analysis of chemical hazards in various fields.
JOURNAL OF MATERIALS CHEMISTRY A
(2021)
Article
Biochemical Research Methods
Victor Cutillas, Carmen Ferrer, Amadeo R. Fernandez-Alba
Summary: The study compared the application of liquid chromatography and supercritical fluid chromatography in pesticide residue analysis. The results showed that SFC performed better in analyzing matrices with a higher number of co-extracted compounds, attributed to the combination of chromatography separation and ion source efficiency.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Kalpana Talari, Sai Krishna Ganji, Mani Kommu, Raja Rajeswari Tiruveedula, Vijayasarathi Upadhyayula
Summary: A simple, selective, and sensitive method based on LC-APCI-HCD-HRMS/MS was developed for quantitative determination of 19 targeted organophosphorus and carbamate pesticides as well as 10 untargeted pesticides in coconut milk samples. The method showed good linearity, sensitivity, and precision, with successful quantification of pesticide residues in the samples.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Mahboob Nemati, Mir Ali Farajzadeh, Mohammad Reza Afshar Mogaddam, Ali Mohebbi, Amir Reza Azimi, Nazir Fattahi, Mustafa Tuzen
Summary: In this study, a new mode of dispersive liquid-liquid microextraction called gas-controlled-deep eutectic solvent-based evaporation-assisted dispersive liquid-liquid microextraction was proposed for the determination of pyrethroid pesticides in fruit juices. By evaporating chloroform and releasing droplets of the extractant, the analytes were extracted into the aqueous phase. The method was optimized and validated, and successfully applied to fruit juice samples, which were found to be free of the selected pesticides.
MICROCHEMICAL JOURNAL
(2022)
Article
Food Science & Technology
Sufang Fan, Junmei Ma, Meirong Cao, Juan Wang, Leilei Zhang, Yan Zhang, Qiang Li, Jia Chen
Summary: A new liquid chromatography tandem mass spectrometry method was developed for the rapid and accurate determination of 15 pesticide residues in Chinese cabbage and cucumber samples. After QuEChERS extraction and online turbulent flow chromatography purification, the recoveries and matrix effects of pesticide residues were within acceptable ranges.
FOOD SCIENCE AND HUMAN WELLNESS
(2021)
Article
Biochemical Research Methods
Souleiman El Balkhi, Franck Saint-Marcoux
Summary: Chlordecone, an organochlorine insecticide used in French West Indies banana fields, has caused persistent pollution of soils and waters. People are mainly exposed to chlordecone through food and studies have shown that this exposure is associated with various health disorders. A new LC-MS/MS method with a low limit of detection has been developed to measure chlordecone in serum.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Agriculture, Multidisciplinary
Zhanli Hu, Jun Li, Yezi Zhang, Yan Shi
Summary: The experiment showed that the residues of diflufenican in wheat and soil were minimal, well below China's maximum residue limit for wheat. The residues decreased with an increase in wheat growth days and soil layers.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Clinical Neurology
Jose A. Pineda-Pardo, Alvaro Sanchez-Ferro, Mariana H. G. Monje, Nicola Pavese, Jose A. Obeso
Summary: The striatal dopaminergic deficit in Parkinson's disease follows a somatotopically related pattern, with an upper-limb onset and progression to other regions of the striatum. The denervation of the putamen was confirmed in both hemispheres and corresponding hemibodies. The findings suggest a precise somatotopic onset of nigrostriatal denervation in Parkinson's disease.
Article
Biochemical Research Methods
Matthew J. Sorensen, Kelsey E. Miller, James W. Jorgenson, Robert T. Kennedy
Summary: In this study, a two-dimensional liquid chromatography-mass spectrometry method was developed for lipidomics analysis of human plasma. The method achieved good orthogonality and high peak capacity, and detected over 1000 lipids from a small amount of plasma sample.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemistry & Molecular Biology
Cristiano Bombardi, Francis Delicata, Claudio Tagliavia, Annamaria Grandis, Massimo Pierucci, Antonella Marino Gammazza, Maurizio Casarrubea, Philippe De Deurwaerdere, Giuseppe Di Giovanni
Summary: Serotonin is crucial in nicotine actions in the CNS, with the 5-HT2CR receptor identified as a potential drug target for smoking cessation. Acute and chronic nicotine exposure have differential effects on the central 5-HT2CR function, primarily in the LHb, which may have implications for nicotine addiction and its treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Editorial Material
Biochemistry & Molecular Biology
Philippe De Deurwaerdere, Abdeslam Chagraoui
Article
Biochemistry & Molecular Biology
Alexandra Gros, Leandre Lavenu, Jean-Luc Morel, Philippe De Deurwaerdere
Summary: The study revealed that microgravity affects brain adaptive responses in rats, particularly the monoaminergic systems, leading to changes in neurotransmitter contents and correlations between brain regions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Massimo Pierucci, Francis Delicata, Roberto Colangeli, Antonella Marino Gammazza, Alessandro Pitruzzella, Maurizio Casarrubea, Philippe De Deurwaerdere, Giuseppe Di Giovanni
Summary: This study found that nicotine-induced neuronal activity in the VTA is regulated by the LHb region, with acute and chronic nicotine having different effects on VTA DA neurons.
Review
Biochemistry & Molecular Biology
Abdeslam Chagraoui, Giuseppe Di Giovanni, Philippe De Deurwaerdere
Summary: The discovery of the D3 receptor has sparked interest in the field of neurological diseases, particularly Parkinson's disease. This article discusses the role of D3 receptors in Parkinson's disease and their relationship with current treatments, highlighting the potential interactions between D1 and D3 receptors that may contribute to motor side effects.
Editorial Material
Biochemistry & Molecular Biology
Philippe De Deurwaerdere, Giuseppe Di Giovanni
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Philippe De Deurwaerdere, Maurizio Casarrubea, Daniel Cassar, Manuela Radic, Emilie Puginier, Abdeslam Chagraoui, Giuseppe Crescimanno, Vincenzo Crunelli, Giuseppe Di Giovanni
Summary: Childhood absence epilepsy is often accompanied by neuropsychological comorbidities such as anxiety. This study found that the synthetic cannabinoid receptor agonist WIN 55,212-2 had strain-dependent effects on anxiety-like and motor behavior in rats with absence epilepsy. The study also revealed alterations in cerebral monoaminergic levels in these rats, suggesting a role of endocannabinoids and monoamines in the comorbidities.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Neurosciences
Fabrizio Sanna, Maria Pina Serra, Marianna Boi, Jessica Bratzu, Laura Poddighe, Francesco Sanna, Antonella Carta, Maria Giuseppa Corda, Osvaldo Giorgi, Maria Rosaria Melis, Antonio Argiolas, Marina Quartu
Summary: Sexual activity causes differential changes in the expression of markers of neural activation and plasticity in the hippocampus of Roman rats, which are influenced by sexual experience level, hippocampal partition, marker type, and rat line.
Article
Biochemistry & Molecular Biology
Virginie Marty, Jasmine J. Butler, Coutens Basile, Oumaima Chargui, Abdeslam Chagraoui, Bruno P. Guiard, Philippe De Deurwaerdere, Jerome Cavaille
Summary: The absence of Snord115 affects central monoaminergic circuits, but does not elicit gross behavioral abnormalities.
HUMAN MOLECULAR GENETICS
(2022)
Article
Multidisciplinary Sciences
Franck Aby, Louis-Etienne Lorenzo, Zoe Grivet, Rabia Bouali-Benazzouz, Hugo Martin, Stephane Valerio, Sara Whitestone, Dominique Isabel, Walid Idi, Otmane Bouchatta, Philippe De Deurwaerdere, Antoine G. Godin, Cyril Herry, Xavier Fioramonti, Marc Landry, Yves De Koninck, Pascal Fossat
Summary: Research has shown that serotonin (5-HT) neurons have a pain-relieving effect in mice who have not experienced pain before, but become pain-promoting in neuropathic pain models. An imbalance in spinal KCC2 function turns this pain relief into pain promotion, but KCC2 enhancers can restore the pain-relieving effect. Additionally, combining selective serotonin reuptake inhibitors (SSRIs) with KCC2 enhancers can effectively relieve pain hypersensitivity caused by nerve injury.
Article
Biochemistry & Molecular Biology
Shima Kouhnavardi, Alev Ecevitoglu, Vladimir Dragacevic, Fabrizio Sanna, Edgar Arias-Sandoval, Predrag Kalaba, Michael Kirchhofer, Jana Lubec, Marco Niello, Marion Holy, Martin Zehl, Matthias Pillwein, Judith Wackerlig, Rita Murau, Andrea Mohrmann, Kathryn R. Beard, Harald H. Sitte, Ernst Urban, Claudia Sagheddu, Marco Pistis, Roberto Plasenzotti, John D. Salamone, Thierry Langer, Gert Lubec, Francisco J. Monje
Summary: Dopamine and DAT signaling dysfunctions are associated with various neuropsychiatric disorders and progressive brain diseases, leading to devastating personal and familial concerns and high socioeconomic costs worldwide.
Review
Neurosciences
Maria Rosaria Melis, Fabrizio Sanna, Antonio Argiolas
Summary: Dopamine plays different roles in male sexual behavior through its neuronal systems and receptor subtypes. Different dopamine systems are involved in different stages of sexual behavior, and D-2 and D-4 receptors play a major role in the pro-sexual effects of dopamine.
Article
Biochemistry & Molecular Biology
Hugo Martin, Sebastien Bullich, Maud Martinat, Mathilde Chataigner, Mathieu Di Miceli, Vincent Simon, Samantha Clark, Jasmine Butler, Mareike Schell, Simran Chopra, Francis Chaouloff, Andre Kleinridders, Daniela Cota, Philippe De Deurwaerdere, Luc Penicaud, Sophie Laye, Bruno P. Guiard, Xavier Fioramonti
Summary: Insulin directly modulates the activity of DR 5-HT neurons to dampen 5-HT neurotransmission and control emotional behaviors. However, in T2D animal models, the anxiolytic effect of intranasal insulin and the response of 5-HT neurons to insulin are both blunted.
MOLECULAR PSYCHIATRY
(2022)
Article
Biochemistry & Molecular Biology
Augusta Pisanu, Giacomo Lo Russo, Giuseppe Talani, Jessica Bratzu, Carlotta Siddi, Fabrizio Sanna, Marco Diana, Patrizia Porcu, Maria Antonietta De Luca, Liana Fattore
Summary: The illicit drug market of novel psychoactive substances is expanding, with 2-Cl-4,5-MDMA and 3,4-MDPHP being suggested as particularly dangerous. This study investigated the abuse liability, corticosterone levels, and dopaminergic transmission interference of these two drugs in adolescent rats. The results showed that the drugs did not lead to reliable self-administration behavior, but chronic exposure to low levels of 3,4-MDPHP increased cortisol levels in females and increased firing rate of dopaminergic neurons in males.
Article
Clinical Neurology
Claudia Sagheddu, Enzo Cancedda, Farshid Bagheri, Predrag Kalaba, Anna Lisa Muntoni, Jana Lubec, Gert Lubec, Fabrizio Sanna, Marco Pistis
Summary: CE-158 potentiates dopamine neurotransmission, promotes behavioral flexibility, and restores learning and memory in rats. Chronic administration of CE-158 does not induce abuse potential or pro-psychotic-like effects. These findings provide important evidence for the clinical application of CE-158.
INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY
(2023)