Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 21, Issue 15, Pages -Publisher
MDPI
DOI: 10.3390/ijms21155269
Keywords
dendrimer; nanopatterning; RGD; mesenchymal cell condensation; cell-cell interactions; YAP; chondrogenesis
Funding
- Networking Biomedical Research Center (CIBER), Spain
- VI National R&D&i Plan 2008-2011
- Iniciativa Ingenio 2010
- Consolider Program
- CIBER Actions
- Instituto de Salud Carlos III [RD16/0006/0012]
- European Regional Development Fund (ERDF)
- CERCA Program
- Commission for Universities and Research of the Department of Innovation, Universities and Enterprise of the Generalitat de Catalunya [2017 SGR 1079]
- ACCIO (Catalonia Trade and Investment
- Generalitat de Catalunya) under the Catalonian ERDF operational program 2014-2020
- Spanish Ministry of Economy and Competitiveness (MINECO) [TEC2015-70104-P, CTQ2016-75870-P, PID2019-104293GB-I00, TEC2015-72718-EXP]
- Spanish Ministry of Science and Innovation [RTI2018-097038-B-C21]
- Consejeria de Salud, Junta de Andalucia [UMA18-FEDERJA-007]
- MINECO
- European Social Fund (2016) [BES-2016-076682]
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Tackling the first stages of the chondrogenic commitment is essential to drive chondrogenic differentiation to healthy hyaline cartilage and minimize hypertrophy. During chondrogenesis, the extracellular matrix continuously evolves, adapting to the tissue adhesive requirements at each stage. Here, we take advantage of previously developed nanopatterns, in which local surface adhesiveness can be precisely tuned, to investigate its effects on prechondrogenic condensation. Fluorescence live cell imaging, immunostaining, confocal microscopy and PCR analysis are used to follow the condensation process on the nanopatterns. Cell tracking parameters, condensate morphology, cell-cell interactions, mechanotransduction and chondrogenic commitment are evaluated in response to local surface adhesiveness. Results show that only condensates on the nanopatterns of high local surface adhesiveness are stable in culture and able to enter the chondrogenic pathway, thus highlighting the importance of controlling cell-substrate adhesion in the tissue engineering strategies for cartilage repair.
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