Review
Sport Sciences
William S. Evans, Ryan M. Sapp, Katherine I. Kim, James M. Heilman, James Hagberg, Steven J. Prior
Summary: This article discusses the benefits of exercise training on cardiovascular health and the role of circulating angiogenic cells in these changes. It emphasizes the essential function of secreted factors from these cells in maintaining cardiovascular health and summarizes the findings of studies on the effects of exercise training on these factors.
INTERNATIONAL JOURNAL OF SPORTS MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Lucia Beltran-Camacho, Margarita Jimenez-Palomares, Ismael Sanchez-Gomar, Antonio Rosal-Vela, Marta Rojas-Torres, Sara Eslava-Alcon, Jose Angel Alonso-Pinero, Almudena Gonzalez-Rovira, Ma Jesus Extremera-Garcia, Rosario Conejero, Esther Doiz, Manuel Rodriguez-Pinero, Martin R. Larsen, Ma Carmen Duran-Ruiz
Summary: The study suggests that circulating angiogenic cells (CACs) have regenerative potential and can enhance blood flow recovery in mice with critical limb ischemia. Pre-stimulation of CACs with factors secreted by atherosclerotic plaques enhances their regenerative properties. Proteomics analysis identified potential proteins involved in the response to ischemia and the effect of CACs administration, indicating a possible mechanism for the therapeutic effects of CACs in CLI.
Article
Hematology
Gregoire Detriche, Nicolas Gendron, Aurelien Philippe, Maxime Gruest, Paul Billoir, Elisa Rossi, Coralie L. Guerin, Anna Lokajczyk, Severine Brabant, Dominique Prie, Tristan Mirault, David M. Smadja
Summary: Gonadotropins increase ECFC-related angiogenesis and may be involved in thrombin generation in cardiovascular disease. Gonadotropins may act as biomarkers; moreover, we hypothesize that gonadotropin-blocking strategies may be a novel interesting therapeutic approach in atherosclerotic cardiovascular disease.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Cell Biology
Shun Hay Pun, Karla M. O'Neill, Kevin S. Edgar, Eleanor K. Gill, Arya Moez, Hojjat Naderi-Meshkin, Sudhir B. Malla, Michelle B. Hookham, Mohammed Alsaggaf, Vinuthna Vani Madishetti, Bianca Botezatu, William King, Coy Brunssen, Henning Morawietz, Philip D. Dunne, Derek P. Brazil, Reinhold J. Medina, Chris J. Watson, David J. Grieve
Summary: This study aimed to investigate and define the contribution of NOX4 NADPH oxidase to hypoxia-induced dysfunction and explore its potential as a therapeutic target. The results showed that activation of the PLAC8-NOX4 signaling axis improved the angiogenic functions of CB-ECFCs under hypoxic conditions.
Article
Cardiac & Cardiovascular Systems
Cheryl M. J. Tan, Adam J. Lewandowski, Wilby Williamson, Odaro J. Huckstep, Grace Z. Yu, Roman Fischer, Jillian N. Simon, Maryam Alsharqi, Afifah Mohamed, Paul Leeson, Mariane Bertagnolli
Summary: This study aimed to compare the proteomic profiles of functional and dysfunctional ECFCs, revealing distinct protein signatures and changes in cellular mechanisms between the two. Dysfunctional ECFCs have impaired angiogenic capacities and are associated with cardiovascular disease risks in young adults.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2021)
Article
Cell Biology
Sanghoon Lee, Rebekah Karns, Soona Shin
Summary: Hepatic progenitor cells (HPCs) play crucial roles in liver diseases and cancer, but their exact role in disease progression is not well understood. Our study found that HPCs communicate with endothelial cells through paracrine factors, promoting angiogenesis and disease progression. Modulation of HPCs differentiation status and inhibition of CSF1 signaling can suppress the paracrine function of HPCs, inhibiting angiogenesis.
CELLULAR SIGNALLING
(2022)
Article
Biochemistry & Molecular Biology
Silvia Erratico, Marzia Belicchi, Mirella Meregalli, Dario Di Silvestre, Luana Tripodi, Antonella De Palma, Rebecca Jones, Emanuele Ferrari, Laura Porretti, Elena Trombetta, Giorgio R. Merlo, Pierluigi Mauri, Yvan Torrente
Summary: Delayed wound healing and chronic skin lesions are major health problems. Growth factors mediated by platelet-rich plasma (PRP) and cell-based therapies have been developed as effective treatments for improving wound healing capacity. This study presents a highly efficient high-throughput protocol for isolating PRP and pro-angiogenic cells (Angio(PRP)) and demonstrates their effectiveness in promoting wound closure, re-epithelialization, granulation tissue growth, and blood vessel regeneration. The mechanical properties of the rescued mouse wounds were also normalized, supported by the correct arrangement of elastin and collagen fibers. Proteomic analysis revealed a distinct fingerprint of Angio(PRP), which was correlated to a decrease in inflammatory response.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell & Tissue Engineering
Audrey Cras, Jerome Larghero, Elisa Rossi, Adeline Blandinieres, Pascale Gaussem, David M. Smadja
Summary: This study reveals that nestin, a protein expressed by ECFCs, plays an important role in their angiogenic properties. Inhibition of nestin reduces ECFC proliferation, migration, tubulogenesis, and adhesion, accompanied by decreased focal adhesion kinase phosphorylation. Furthermore, nestin silencing leads to reduced revascularization in a mouse hindlimb ischemia model.
STEM CELL REVIEWS AND REPORTS
(2023)
Article
Chemistry, Multidisciplinary
Min Ji Han, Jeong Ah An, Jeong Min Kim, Dong Nyoung Heo, Il Keun Kwon, Kyung Min Park
Summary: Polymeric hydrogels are used as therapeutic vehicles for delivering cells or bioactive molecules to enhance bone tissue regeneration. This study reports on gelatin-based bioactive matrices that can serve as dual carriers of oxygen and calcium ions for vascularized bone tissue regeneration. The bioactive hydrogels have controllable mechanical properties and can release calcium ions and oxygen in a sustained manner, promoting cell proliferation and vascularization. The hydrogels enhance the proliferation of osteoblasts and gene expression of vascular endothelial growth factor, showing potential for vascularized bone tissue regeneration.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Konstantin Dergilev, Ekaterina Zubkova, Alika Guseva, Zoya Tsokolaeva, Yulia Goltseva, Irina Beloglazova, Elizaveta Ratner, Alexander Andreev, Stanislav Partigulov, Mikhail Lepilin, Mikhail Menshikov, Yelena Parfyonova
Summary: Ischemic heart disease and its complications are the leading causes of death in modern society. Stem-cell-based transplantation, particularly using cardiosphere-derived cells (CDCs), is considered a promising treatment for damaged myocardial tissue. The paracrine effect of transplanted cells, through the secretion of bioactive molecules, plays a crucial role in the therapeutic effect. However, the use of secretome-based therapies still faces obstacles due to population heterogeneity and limited understanding of secretome regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Nadia Meyer, Lars Brodowski, Katja Richter, Constantin S. von Kaisenberg, Bianca Schroeder-Heurich, Frauke von Versen-Hoeynck
Summary: Pravastatin enhances the functional capacities of ECFCs and promotes angiogenic signaling and protein expression, improving endothelial health. These effects may offer a promising option for ameliorating endothelial dysfunction and understanding the pleiotropic function of statins.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Nezar Boreak, Noor Mohammed Abu Khayrat, Ahmed Omar Shami, Haitham Jameel Mohammed Zaylaee, Aeshah Ali Hanbashi, Sara Abdulhamed Souri, Hala Mohammed Otayf, Rafif Essam Bakri, Mohammad Eissa Mansoor Ajeely, Alwaleed Essam Hassan Bakri, Mohammed Abdurabu Jafer, A. Thirumal Raj, Hosam Ali Baeshen, Shankargouda Patil
Summary: The study demonstrated that treatment with metformin enhances the angiogenic potential of dental pulp stem cell secretome, potentially aiding in regeneration.
SAUDI PHARMACEUTICAL JOURNAL
(2021)
Article
Cardiac & Cardiovascular Systems
Wenhui Yang, Haizhen Wang, Qiuzhe Guo, Xiaocui Xu, Tao Guo, Lin Sun
Summary: By enhancing the activity of CACs, TRPV4 can regulate angiogenesis and improve therapeutic outcomes in the area of myocardial infarction.
JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH
(2023)
Article
Obstetrics & Gynecology
Naoko Sasamoto, Long Ngo, Allison F. Vitonis, Simon T. Dillon, Stacey A. Missmer, Towia A. Libermann, Kathryn L. Terry
Summary: The study investigated systemic molecular profiles of endometriosis in adolescents and young adults, identifying significant enrichment of proteins related to angiogenesis and cell migration pathways in endometriosis cases. Increased activation of these pathways was observed in endometriosis cases compared to controls. Results suggest the need for further validation and mechanistic studies to better understand the pathophysiological characteristics of this understudied patient population.
HUMAN REPRODUCTION
(2022)
Article
Cell & Tissue Engineering
Young Cheol Song, Gyu Tae Park, Hye Ji Moon, Eun-Bae Choi, Mi-Ju Lim, Jung Won Yoon, Nayeon Lee, Sang Mo Kwon, Byung-Joo Lee, Jae Ho Kim
Summary: Hybrid cell spheroids can enhance the survival and therapeutic effects of cell transplantation for peripheral artery disease.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Neurosciences
Eduardo Molina-Holgado, Pedro F. Esteban, Angel Arevalo-Martin, Rafael Moreno-Luna, Francisco Molina-Holgado, Daniel Garcia-Ovejero
Summary: Oligodendrocytes in the central nervous system synthesize myelin and have the ability to regulate their functions through interactions with other cells and sensing the environment. They respond to various signals, including the endocannabinoid signaling, which has therapeutic potential for promoting remyelination in central nervous system pathologies.
Article
Biochemistry & Molecular Biology
Lucia Beltran-Camacho, Sara Eslava-Alcon, Marta Rojas-Torres, Daniel Sanchez-Morillo, Ma Pilar Martinez-Nicolas, Victoria Martin-Bermejo, Ines Garcia de la Torre, Esther Berrocoso, Juan Antonio Moreno, Rafael Moreno-Luna, Ma Carmen Duran-Ruiz
Summary: This study evaluated the impact of serum from asymptomatic COVID-19 individuals on circulating angiogenic cells (CACs) using a proteomics-based quantitative approach. The results confirmed that SARS-CoV-2 infection in asymptomatic individuals caused protein level changes related to coagulation and inflammatory processes. Additionally, certain proteins were up-regulated only in CACs treated with serum from asymptomatic patients during the highest peak of infection. These findings provide insight into the effects of SARS-CoV-2 infection and the development of potential treatment methods.
MOLECULAR MEDICINE
(2022)
Article
Cell Biology
Mario Martinez-Torija, Pedro F. Esteban, Francisco Javier Espino-Rodriguez, Beatriz Paniagua-Torija, Eduardo Molina-Holgado, Silvia Ceruelo, Gemma Barroso-Garcia, Alba G. Arandilla, Luis F. Lopez-Almodovar, Angel Arevalo-Martin, Juan Antonio Moreno, Daniel Garcia-Ovejero, M. Carmen Duran-Ruiz, Rafael Moreno-Luna
Summary: The risk of complications following surgical procedures is significantly increased in patients with SARS-CoV-2 infection. This study found that SCI patients with a COVID-19-positive diagnosis showed worse recovery and severe postoperative complications. Proteomic analysis revealed several upregulated proteins in the adipose tissue surrounding the ulcers. Among them, CKMT2 and CKM stood out and increased after the COVID-19 diagnosis. These proteins were largely found in MGCs within the adipose tissue. The presence of CKMT2, CKM, and MGCs may serve as predictive biomarkers of postsurgical complications in post-COVID patients.
Article
Biochemistry & Molecular Biology
Ismael Sanchez-Gomar, Josefa Benitez-Camacho, Cristina Cejudo-Bastante, Lourdes Casas, Rafael Moreno-Luna, Casimiro Mantell, Ma Carmen Duran-Ruiz
Summary: The study evaluated the potential of mango leaves, olive leaves, and red grape pomace extracts rich in polyphenols to promote ECFC reparative effects. It was found that ethanolic extracts at low concentrations improved angiogenic capacities of ECFCs and reduced proliferation, apoptosis, and the inflammatory response of these cells.
Article
Cell & Tissue Engineering
Marta Rojas-Torres, Ismael Sanchez-Gomar, Antonio Rosal-Vela, Lucia Beltran-Camacho, Sara Eslava-Alcon, Jose Angel Alonso-Pineiro, Javier Martin-Ramirez, Rafael Moreno-Luna, Ma Carmen Duran-Ruiz
Summary: Before translating ECFCs into clinical treatment, it is necessary to perform biodistribution assays to ensure biosafety and determine the distribution of cells. This study optimized a cell-tracking assay using DiR labeling and qPCR analysis, and found that intramuscularly administered ECFCs mainly remained in the ischemic tissues, while intravenously injected cells were found in multiple organs.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Polymer Science
Pilar Grosso, Cristina Cejudo, Ismael Sanchez-Gomar, M. Carmen Duran-Ruiz, Rafael Moreno-Luna, Lourdes Casas, Clara Pereyra, Casimiro Mantell
Summary: The addition of natural substances with pharmacoactive properties to polymeric biomedical devices can enhance the assimilation of these implants and promote blood vessel formation. In this study, mango leaf extract (MLE) obtained through pressurized ethanol was impregnated into polylactic acid (PLA) devices. The best impregnation conditions and results were determined through in vitro culture experiments and microscopy observations.
Article
Biology
Alejandra Sanchez-Brinas, Carmen Duran-Ruiz, Antonio Astola, Marta Marina Arroyo, Fatima G. Raposo, Antonio Valle, Jorge Bolivar
Summary: Research reveals that NOA36 interacts with HSPA1 and HSPA8 heat shock proteins, and plays a crucial role in cell proliferation and cell cycle.
Article
Biochemistry & Molecular Biology
Diego Valor, Antonio Montes, Maria Calderon-Dominguez, Inass Aghziel, Ismael Sanchez-Gomar, Martin Alcala, Ma Carmen Duran-Ruiz, Clara Pereyra
Summary: Submicron particles were produced from an ethanolic extract of Myrtus communnis leaves using supercritical carbon dioxide technology. The particles had small sizes, quasi-spherical shape, and high antioxidant activity. It was also observed that increasing pressure and temperature resulted in smaller particle sizes.
Article
Biochemistry & Molecular Biology
Lucia Beltran-Camacho, Santosh D. Bhosale, Daniel Sanchez-Morillo, Ismael Sanchez-Gomar, Marta Rojas-Torres, Sara Eslava-Alcon, Mario Martinez-Torija, M. Angeles Ruiz de Infante, M. Dolores Nieto-Martin, Manuel A. Rodriguez-Iglesias, Juan A. Moreno, Esther Berrocoso, Martin R. Larsen, Rafael Moreno-Luna, M. Carmen Duran-Ruiz
Summary: SARS-CoV-2 infection affects the cardiovascular system, causing vascular damage and thromboembolic events. Endothelial dysfunction is an early response to COVID-19, which may lead to cardiovascular complications and long-term sequelae. However, the molecular mechanisms involved in these processes remain poorly understood.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)