Article
Ophthalmology
Yifang Yang, Junshu Wu, Defu Wu, Qi Wei, Tan Zhong, Jun Yang, Xiaowei Yang, Meizhen Zeng, Xingwu Zhong
Summary: Among the 3 different administration methods, intravitreal injection of brimonidine was the most effective in slowing myopia progression in the FD guinea pig model. Intravitreal brimonidine at 4 mu g/mu L significantly reduced the development of FD myopia in guinea pigs. Expression levels of the Col1a1 and Mmp2 genes were significantly increased in the retinal tissues of the FD-Inj-Br group.
Article
Oncology
Lu Tian, Ya-Tu Guo, Ming Ying, Yang-Chen Liu, Xuan Li, Yan Wang
Summary: In this study, it was found that long periods of monocular form deprivation in guinea pigs resulted in significant refractive errors and increased axial lengths in the deprived eyes. Myopia was established at 4 weeks, while amblyopia may have occurred as early as 1 week of deprivation. This suggests that amblyopia likely precedes myopia and both conditions coexist after prolonged form deprivation in guinea pigs.
ANNALS OF TRANSLATIONAL MEDICINE
(2021)
Article
Cell Biology
Yajun Wu, Yuliang Feng, Jiasong Yang, Hua Fan, Zitong Yu, Xiaolin Xie, Yumeng Dai, Xin Huang, Wensheng Li
Summary: The aim of this study was to investigate the effects of exogenous retinoic acid (RA) on intraocular parameters, especially choroidal thickness (CT) and retinal thickness (RT), in guinea pigs with form deprivation myopia (FDM). After 4 weeks, it was found that RA can increase the refractive error, axial length, and intraocular pressure of FDM guinea pigs, and might aggravate the retinal thinning. Citral, on the other hand, can inhibit these changes, but may not affect the thickness of the choroid.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Ophthalmology
Lei Guo, Rui Hua, Xinxin Zhang, Ting Yu Yan, Yang Tong, Xin Zhao, Shi Chao Chen, Moying Wang, Neil M. Bressler, Jun Kong
Summary: The study finds that scleral cross-linking may cause glaucomatous changes in guinea pig eyes and raises safety concerns for controlling myopia.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Ophthalmology
Hong Liu, Donglong Chen, Zhikuan Yang, Xiaoning Li
Summary: The study aimed to investigate the morphological and functional changes caused by atropine during myopia inhibition. It was found that there were significant differences between the atropine group and the saline group in terms of refraction error, axial length, and retinal and choroidal thickness. Atropine may mainly affect the outer retina in guinea pigs during the process of inhibiting form-deprived myopia.
CURRENT EYE RESEARCH
(2022)
Article
Ophthalmology
Liqin Jiang, Xinyu Liu, Lei Zhou, Joanna M. Fianza Busoy, Myo Thu Khine, Yee Shan Dan, Mengyuan Ke, Noel A. Brennan, Karen J. Catbagan, Leopold Schmetterer, Veluchamy A. Barathi, Quan Hoang
Summary: This study aimed to investigate the relationship between choroidal characteristics and susceptibility to myopia development. The results showed that greater choroidal thickness could protect against myopia, while lower choroidal thickness was associated with a higher susceptibility to myopia development. Dysfunction of the choroidal vascular system may be the underlying mechanism.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Ophthalmology
Man She, Bing Li, Tao Li, Xiaodong Zhou
Summary: The expression levels of AREG in the sclera of guinea pigs increased during the development of FDM and downregulated after recovery of FDM. Therefore, AREG may play a role in regulating scleral remodeling in myopia.
CURRENT EYE RESEARCH
(2022)
Article
Ophthalmology
Guimei Zhou, Changjun Lan, Qin Yang, Weiqi Zhong, Zhiming Gu, Xiaoling Xiang, Yumei Mao, Xuan Liao
Summary: The study found that after four weeks of form deprivation, the refractive degree and axial length of guinea pigs significantly increased, the retinal and scleral tissues became thinner, and the protein and mRNA expression levels of SCO1 and SCO2 were significantly reduced in the FDM group.
EUROPEAN JOURNAL OF OPHTHALMOLOGY
(2022)
Article
Ophthalmology
Qian Fu, Yue Zhang, Linji Chen, Mengmeng Dong, Wenyu Tang, Si Chen, Jia Qu, Xiangtian Zhou, Zhina Zhi
Summary: This study establishes a model for near work myopia induced by short viewing distance in guinea pigs and evaluates the effect of viewing distance on myopia development. The findings suggest that short viewing distance could be a risk factor for myopia and provide a useful model for studying the underlying mechanisms.
EXPERIMENTAL EYE RESEARCH
(2022)
Article
Ophthalmology
Wei Chen, Li Li, Qiang Feng, Chen Xi Li, Yue Zhang, Zhi Wei Li
Summary: The study revealed that changes in choroidal vessel diameter (CVD) and nitric oxide synthase (NOS) during the recovery of form-deprivation myopia in guinea pigs are closely associated with choroidal thickness (CT).
CURRENT EYE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Zedu Cui, Yuke Huang, Xi Chen, Taiwei Chen, Xiangtao Hou, Na Yu, Yan Li, Jin Qiu, Pei Chen, Keming Yu, Jing Zhuang
Summary: By comparing two miRNA datasets, miR-671-5p was identified as a commonly downregulated miRNA in the retina. It is highly conserved and related to 40.78% of all downregulated miRNAs' target genes. Furthermore, 584 target genes of miR-671-5p were found to be associated with myopia, and 8 hub genes were identified. The study also revealed the important role of miR-671-5p in myopia development and identified Tead1 as a possible upstream regulator of miR-671-5p.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Ophthalmology
Manrong Yu, Wangyuan Liu, Bingjie Wang, Jinhui Dai
Summary: The study showed that guinea pigs exposed to short-wavelength light were less likely to develop myopia compared to those under white light, with shorter axial length and lower retinal RA levels. Oral RA supplementation accelerated eye elongation, but the synthesis inhibitor citral equally slowed down eye growth under different light conditions, with no significant reduction in retinal RA levels.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Ophthalmology
Miaozhen Pan, Zhenqi Guan, Peter S. Reinach, Lin Kang, Yuqing Cao, Dengke Zhou, Nethrajeith Srinivasalu, Fei Zhao, Jia Qu, Xiangtian Zhou
Summary: PPAR gamma modulates the progression of form deprivation myopia by influencing choroidal thickness, choroidal blood perfusion, and scleral hypoxia-inducible transcription factor, which in turn regulate scleral collagen type 1 expression levels.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Ophthalmology
Hongping Xu, Yan Dong, Fen He, Bo Qin
Summary: This study explored the association between melanopsin expression and the development of myopia. The results showed that melanopsin expression decreased in the retina during form deprivation and lens-induced myopia, but partially recovered after the removal of the deprivation or lens. These findings suggest that decreased melanopsin expression may be involved in the development of myopia.
CURRENT EYE RESEARCH
(2023)
Article
Ophthalmology
Qiong Wang, Xiaowen Ji, Daqian Lu, Ying Zhu, Amy Whelchel, Jingyi Wang, Hongmei Zhang, Lijie Dong, Ruihua Wei
Summary: The Lenstar optical biometer showed better repeatability and provided smaller measurements for Delta L, VCD, and ASD compared to A-scan ultrasonography. There was a high correlation between the two devices in measuring Delta AL. While the devices did not agree in absolute measurements, the Lenstar can still be considered as an option in measuring the difference in AL between eyes in a guinea pig myopia model.
EXPERIMENTAL EYE RESEARCH
(2021)
Review
Nanoscience & Nanotechnology
Yue Gao, Chengyan Chu, Anna Jablonska, Jeff W. M. Bulte, Piotr Walczak, Miroslaw Janowski
Summary: Extracellular vesicles (EVs) have garnered attention as potential therapeutic agents, especially in the field of central nervous system diseases. Successful clinical studies require more information on the biodistribution and tracking of EVs in vivo. Advanced imaging techniques play a crucial role in facilitating the clinical translation of EV-based therapeutics.
WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
(2021)
Article
Neurosciences
Sylwia Dabrowska, Anna Andrzejewska, Hanna Kozlowska, Damian Strzemecki, Miroslaw Janowski, Barbara Lukomska
Summary: The study examined the early immune response in deep brain lacunar infarct, revealing a surge of neurodegenerative changes and increased infiltration of immune cells following brain injury. Higher levels of pro-inflammatory cytokines and chemokines were found in the injured brain, while anti-inflammatory cytokines were lower compared to normal rats. These findings shed new light on potential stroke immunotherapies targeting the acute immune response.
EXPERIMENTAL NEUROLOGY
(2021)
Editorial Material
Clinical Neurology
Dheeraj Gandhi, Miroslaw Janowski
Article
Geriatrics & Gerontology
Johannes Boltze, Jaroslaw A. Aronowski, Jerome Badaut, Marion S. Buckwalter, Mateo Caleo, Michael Chopp, Kunjan R. Dave, Nadine Didwischus, Rick M. Dijkhuizen, Thorsten R. Doeppner, Jens P. Dreier, Karim Fouad, Mathias Gelderblom, Karen Gertz, Dominika Golubczyk, Barbara A. Gregson, Edith Hamel, Daniel F. Hanley, Wolfgang Haertig, Friedhelm C. Hummel, Maulana Ikhsan, Miroslaw Janowski, Jukka Jolkkonen, Saravanan S. Karuppagounder, Richard F. Keep, Inga K. Koerte, Zaal Kokaia, Peiying Li, Fudong Liu, Ignacio Lizasoain, Peter Ludewig, Gerlinde A. S. Metz, Axel Montagne, Andre Obenaus, Alex Palumbo, Monica Pearl, Miguel Perez-Pinzon, Anna M. Planas, Nikolaus Plesnila, Ami P. Raval, Maria A. Rueger, Lauren H. Sansing, Farida Sohrabji, Charlotte J. Stagg, R. Anne Stetler, Ann M. Stowe, Dandan Sun, Akihiko Taguchi, Mickael Tanter, Sabine U. Vay, Raghu Vemuganti, Denis Vivien, Piotr Walczak, Jian Wang, Ye Xiong, Marietta Zille
Summary: The past decade has witnessed significant advancements in both diagnosis and treatment of cerebrovascular diseases, with the introduction of thrombectomy in ischemic stroke and the identification of new therapeutic targets based on deeper understanding of pathomechanisms. Additionally, translational aspects are increasingly recognized in basic science studies, enhancing their predictive value for clinical application.
FRONTIERS IN AGING NEUROSCIENCE
(2021)
Review
Chemistry, Multidisciplinary
Anna Andrzejewska, Sylwia Dabrowska, Barbara Lukomska, Miroslaw Janowski
Summary: Neurological disorders pose a growing burden as society ages, with stem cell-based regenerative medicine offering a promising approach. Researchers are studying the application of MSCs to various neurological diseases and focusing on the delivery of stem cells to the brain as an essential and understudied challenge.
Review
Immunology
Sylwia Dabrowska, Anna Andrzejewska, Miroslaw Janowski, Barbara Lukomska
Summary: Mesenchymal stem cells (MSCs) possess supportive, differentiating, and immunoregulatory functions, while the extracellular vesicles (EVs) they produce can serve as a potential cellular therapy tool.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Rui Wang, Chengyan Chu, Zhiliang Wei, Lin Chen, Jiadi Xu, Yajie Liang, Miroslaw Janowski, Robert D. Stevens, Piotr Walczak
Summary: Cell transplantation-based treatments for neurological disease show promise, but graft rejection remains a major hurdle. This study demonstrates that co-stimulation blockade can promote long-term survival of transplanted stem cells in the brain, even post-TBI, potentially enhancing post-injury brain tissue repair.
JOURNAL OF NEUROINFLAMMATION
(2021)
Article
Biochemical Research Methods
Chengyan Chu, Anna Jablonska, Yue Gao, Xiaoyan Lan, Wojciech G. Lesniak, Yajie Liang, Guanshu Liu, Shen Li, Tim Magnus, Monica Pearl, Miroslaw Janowski, Piotr Walczak
Summary: Using MRI guidance, the variability in BBB manipulation can be greatly reduced or eliminated, leading to improved delivery of therapeutic agents to the brain. This technique is easy to implement and can enhance the efficacy of treatment.
Article
Chemistry, Multidisciplinary
Alexandra Chovsepian, Daniel Berchtold, Katarzyna Winek, Uta Mamrak, Ines Ramirez Alvarez, Yanina Dening, Dominika Golubczyk, Luis Weitbrecht, Claudia Dames, Marine Aillery, Celia Fernandez-Sanz, Zdzislaw Gajewski, Marianne Dieterich, Miroslaw Janowski, Peter Falkai, Piotr Walczak, Nikolaus Plesnila, Andreas Meisel, Francisco Pan-Montojo
Summary: The study demonstrates that glycolic acid has a significant protective effect during ischemic reperfusion, reducing ischemic damage and improving functional outcomes. This protective effect may be achieved by counteracting the glutamate-induced increase in intracellular calcium.
Article
Cell Biology
Luiza Stanaszek, Malgorzata Majchrzak, Katarzyna Drela, Piotr Rogujski, Joanna Sanford, Michal Fiedorowicz, Magdalena Gewartowska, Malgorzata Frontczak-Baniewicz, Piotr Walczak, Barbara Lukomska, Miroslaw Janowski
Summary: In this study, canine GRPs were transplanted into the lateral ventricles of neonatal mice, with posterior transplantation significantly extending animal survival compared to anterior transplantation. However, anteriorly transplanted animals exhibited a higher level of myelination in the corpus callosum.
Article
Biochemistry & Molecular Biology
Antonina M. Araszkiewicz, Eduarda P. Oliveira, Terje Svendsen, Katarzyna Drela, Piotr Rogujski, Izabela Malysz-Cymborska, Michal Fiedorowicz, Rui L. Reis, Joaquim Miguel Oliveira, Piotr Walczak, Miroslaw Janowski, Barbara Lukomska, Luiza Stanaszek
Summary: Cell transplantation is a promising strategy for treating neurological disorders, but its effectiveness is limited by various challenges. This study developed alginate-based hydrogels as cell carriers, which improved the delivery accuracy and efficacy of transplanted cells. These hydrogels also enabled non-invasive magnetic resonance imaging (MRI) for monitoring the fate of the cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Luiza Stanaszek, Piotr Rogujski, Katarzyna Drela, Michal Fiedorowicz, Piotr Walczak, Barbara Lukomska, Miroslaw Janowski
Summary: Amyotrophic lateral sclerosis (ALS) is a fatal disease with no effective therapy. This study investigated the transplantation of stem cells into immunodeficient mice with ALS, but did not find any therapeutic benefits in terms of animal survival or neurodegeneration. The lack of positive effects might be due to the inability of the transplanted cells to prevent cell loss.
Review
Oncology
Ruman Rahman, Miroslaw Janowski, Clare L. Killick-Cole, William G. B. Singleton, Emma Campbell, Piotr Walczak, Soumen Khatua, Lukas Faltings, Marc Symons, Julia R. Schneider, Kevin Kwan, John A. Boockvar, Steven S. Gill, J. Miguel Oliveira, Kevin Beccaria, Alexandre Carpentier, Michael Canney, Monica Pearl, Gareth J. Veal, Lisethe Meijer, David A. Walker
Summary: Brain tumors are the leading cause of cancer deaths in children and young adults, but drug treatments have had limited success due to the blood-brain barrier. This review explores techniques to enhance drug delivery to brain tumors and discusses preclinical models for translational development. It also highlights the potential of existing approved drugs when combined with efficient delivery methods. This research aims to address the current challenges faced by children with brain tumors and improve treatment outcomes.
Article
Pharmacology & Pharmacy
Jing Liu, Chengyan Chu, Jia Zhang, Chongxue Bie, Lin Chen, Safiya Aafreen, Jiadi Xu, David O. Kamson, Peter C. M. van Zijl, Piotr Walczak, Miroslaw Janowski, Guanshu Liu
Summary: The purpose of this study was to develop a label-free MRI approach using CEST technology to detect intracranial accumulation of mannitol following OBBBO. The results showed that CEST MRI could effectively detect mannitol in the brain and may be used to optimize the dosing of mannitol-based OBBBO to improve its safety and efficacy.
Article
Biotechnology & Applied Microbiology
Silvia Vieira, Paulina Strymecka, Luiza Stanaszek, Joana Silva-Correia, Katarzyna Drela, Michal Fiedorowicz, Izabela Malysz-Cymborska, Miroslaw Janowski, Rui Luis Reis, Barbara Lukomska, Piotr Walczak, Joaquim Miguel Oliveira
Summary: This study aims to develop a stable injectable Mn-based methacrylated gellan gum (Mn/GG-MA) hydrogel for real-time monitored cell delivery into the central nervous system. By supplementing GG-MA solutions with paramagnetic Mn2+ ions, the hydrogel can be visualized under Magnetic Resonance Imaging (MRI). Cell-laden hydrogels prepared using the Mn/GG-MA formulations remained viable after 7 days of culture. In vivo tests on immunocompromised mice showed that the injection of Mn/GG-MA solutions resulted in a continuous and traceable hydrogel visible on MRI scans. Overall, the developed formulations are suitable for non-invasive cell delivery techniques and image-guided neurointerventions.
BIOENGINEERING-BASEL
(2023)