Silver sulfadiazine-loaded electrospun ethyl cellulose/polylactic acid/collagen nanofibrous mats with antibacterial properties for wound healing

Recently, the electrospun nanofibermats with appropriate properties for applications in the biomedical area has been more considered. In this regard, we successfully fabricated a novel antibacterial nanofibermat (ethyl cellulose/poly lactic acid/collagen) (EC/PLA/collagen) incorporated with silver sulfadiazine (AgSD) and then analyzed with the required tests. AgSD was loaded in the developed mats with different contents (0.25%, 0.5% and 0.75%) and then electrospun to prepare nanofiber mats. To check the chemical structure of the developed mat, Fourier transform infrared spectroscopy (FTIR) was assessed. Surface morphological studies were performed by Scanning Electron Microscopy (SEM), which displayed uniform nanofiber mats without any bead formation. When the hydrophilicity was enhanced by decreasing the blending ratios of EC/PLA, the thermal stability of the nano-fibers was reduced. The water contact angle (WCA) of NFs enhanced by decreasing the blending ratios of EC/PLA. The antibacterial properties showed the inhibition activity against Bacillus (9.71 +/- 1.15mm) and E. coli (12.46 +/- 1.31 mm) bacteria. Besides, nanofibers have improved cell proliferation and adhesion with any cytotoxic effect on NIH 3T3 fibroblast cells. According these results, it seems that the developed mats would be effective scaffold for application in wound dressings. (C) 2020 Published by Elsevier B.V.