4.7 Article

Ulvan lyase assisted structural characterization of ulvan from Ulva pertusa and its antiviral activity against vesicular stomatitis virus

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ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.04.187

Keywords

Ulva pertusa; Sulfated polysaccharide; Ulvan lyase; Structure; Antiviral activity

Funding

  1. Natural Science Foundation of China [31700755]
  2. Key Research and Development Program of Shandong Province [2016CYJS08A01-1]
  3. Key Project of New and Old Energy Transformation in Shandong Province

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Marine green algae arc valuable sources of diverse health-promoting bioactive components. Ulvan is suitable for biological applications due to its unique structure and numerous bioactivities. Here, the complex structure of ulvan from Ulva pertusa was analyzed using specific ulvan lyase degradation, MS, and NMR detection. Its structure mainly consists of -> 4)-beta-D-GlcA-(1 -> 4)-alpha-L-Rha3S-(1 -> and -> 4)-beta-D-Xyl-( 1 -> 4 )-alpha-L-Rha3S-(1 -> repeating units. Small amounts of -> 4)-alpha-L-IdoA-(1 -> 4)-alpha-L-Rha3S-(1 -> unit also exist. In addition, a minor number of branches, a single GIcA, and a long branch containing GIcA-Gk were linked to Rha3S. The antiviral activity of the ulvan and its degraded fragments were further investigated. Ulvan (1068.2 kDa) and ulvan-F1 (38.5 kDa) with relatively high molecular weight showed potency of inhibiting the infection and replication of vesicular stomatitis virus (VSV) at 100 mu g/mL, the inhibition rate of VSV replication was 40.75% and 40.13%, respectively. These results indicated that ulvan has potential as a functional agent. (C) 2020 Elsevier B.V. All rights reserved.

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