Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 155, Issue -, Pages 625-635Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.03.236
Keywords
Double crosslinked hydrogel; Human-like collagen; Wound repair
Funding
- National Natural Science Foundation of China [21838009, 21878247]
- Key RAMP
- D Program of China [2019YFA0905200]
- Shaanxi Key Laboratory of Degradable Biomedical Materials Program [17JS126]
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Skin defects caused by various reasons are currently common clinical problems. At present, hydrogels have been proposed as tissue-engineered skin scaffolds to regenerate the tissues of the defect. We used human-like collagen (HLC), which was isolated and purified after high-density fermentation of recombinant E. coli BL21 The gel uses HLC and carboxymethylated chitosan (CCS) as raw materials and combines enzyme-chemical double cross-linking technology to form a three-dimensional porous network structure that mimics the human extracellular matrix, providing attachment points and nutrients for cell growth and proliferation. For comparison, we used a common hydrogel raw material, gelatin, to prepare a hydrogel in the same way. The experimental results show that the HLC-CCS skin scaffold hydrogel has good mechanical properties, high porosity and good histocompatibility. And full-thickness skin defect repair experiments showthat this hydrogel has a good ability to promote skin tissue regeneration at the wound. In summary, this HLC-based double-crosslinked hydrogel can be used as a project strategy for skin defect repair. (C) 2020 Elsevier B.V. All rights reserved.
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