Article
Fisheries
Chang-Hong Cheng, Xiu-Ze Liu, Hong-Ling Ma, Guang-Xin Liu, Yi-Qin Deng, Juan Feng, Yu-Kun Jie, Zhi-Xun Guo
Summary: Sp-Caspase 3 plays important roles in the immune response and apoptosis against bacterial infection in the mud crab.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Chang-Hong Cheng, Yu Tian, Hong-Ling Ma, Guang-Xin Liu, Si-Gang Fan, Yi-Qin Deng, Jian-Jun Jiang, Juan Feng, Zhi-Xun Guo
Summary: This study cloned and characterized a novel HSC70 protein from the mud crab, and found that it plays an important role in defense against V. parahaemolyticus infection by activating immune response and antioxidant defense signaling pathways.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Immunology
Yunfei Zhang, Yongyong Lai, Xiujuan Zhou, Fei Zhu
Summary: In this study, the researchers found that miR-133 is significantly up-regulated in mud crabs after infection with WSSV or V. parahaemolyticus. Knockdown of miR-133 increased the viral copy number and mortality rate in infected crabs, while also enhancing the expression of astakine. This indicates that miR-133 is involved in the immune response to viral or bacterial infection in mud crabs.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
ChangHong Cheng, HongLing Ma, GuangXin Liu, SiGang Fan, YiQin Deng, JianJun Jiang, Juan Feng, ZhiXun Guo
Summary: In this study, a novel Nrf2 gene was identified in the mud crab, which plays important roles in detoxification and antioxidant enzymes against oxidative stress. The Nrf2 signaling pathway was found to be involved in the response to bacterial infection. Over-expression of Nrf2 improved cell viability under oxidative stress, while silencing Nrf2 increased mortality in response to bacterial infection.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Chang-Hong Cheng, Hai-Tao Ma, Hong-Ling Ma, Guang-Xin Liu, Yi-Qin Deng, Juan Feng, Li-Cang Wang, Ying-Ying Cheng, Zhi-Xun Guo
Summary: The tumor suppressor protein p53, encoded by the Sp-53 gene in mud crab, plays important roles in response to Vibrio parahaemolyticus infection, including regulation of antioxidant defense, DNA repair, and apoptosis. Knockdown of Sp-53 resulted in reduced expression of certain genes and increased mortality and DNA damage in mud crabs challenged by V. parahaemolyticus, highlighting the significance of Sp-53 in host defense mechanisms.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2021)
Article
Fisheries
Xiongchao Ma, Fei Zhu
Summary: Myosin-9 plays a key role in the immune response of S. paramamosain against WSSV and V. alginolyticus, affecting the expression of immune-related genes and the antiviral capacity of crab hemocytes when knocked down.
Article
Biotechnology & Applied Microbiology
Shanquan Liang, Tan Zhang, Zhihao Liu, Jingyu Wang, Changliang Zhu, Qing Kong, Xiaodan Fu, Haijin Mou
Summary: Vibrio parahaemolyticus demonstrates complex survival mechanisms under high hydrostatic pressure, including morphological changes and differential gene expression. Under 20 MPa conditions, genes related to energy metabolism were downregulated, while genes related to adhesion and virulence factors were upregulated, with a response mechanism involving RNA polymerase sigma factor RpoE.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Qimin Wu, Xue Li, Tingting Zhang, Miaomiao Zhang, Xingfan Xue, Wenhui Yang, Lingfei Hu, Zhe Yin, Dongsheng Zhou, Yuyu Sun, Renfei Lu, Yiquan Zhang
Summary: Vibrio parahaemolyticus can naturally and reversibly switch between wrinkly and smooth phenotypes, and this process is strictly regulated by a complex molecular regulatory network. Significantly differentially expressed genes are involved in various biological behaviors during this process.
MICROBIOLOGY SPECTRUM
(2022)
Article
Fisheries
Qingyang Wu, Yang Jiang, Enzhu Chen, Changkao Mu, Khor Waiho
Summary: The study found that Galla chinensis exhibited the highest antibacterial effect on the pathogen Vibrio parahaemolyticus, suggesting its potential use in treating bacterial diseases in mud crabs.
Article
Food Science & Technology
Xingguo Zhang, Wendan Shangguan, Jie Wang, Zhenlin Liao, Xiang Fang, Qingping Zhong
Summary: This study discovered novel anti-biofilm agents from Lacticaseibacillus rhamnosus. The ethyl acetate extract of L. rhamnosus MS1 was found to effectively reduce the motility, extracellular polysaccharide (EPS) content, and biofilm production of Vibrio parahaemolyticus. Transcriptomic analysis revealed significant gene expression changes in V. parahaemolyticus treated with the extract, indicating the regulation of quorum sensing, biofilm formation, virulence factors, and metabolic pathways by L. rhamnosus MS1. This study demonstrates the efficacy of L. rhamnosus MS1 as an anti-biofilm agent.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Fisheries
Zhao-Zhe Xin, Xin-Tong Zhang, Zhi-Qiang Zhu, Jiu-Yang Chen, Jin-Yong Zhang
Summary: Microsporidiosis in aquaculture has caused severe economic losses. Understanding the molecular mechanism of host responses to aquatic microsporidia infection is important for controlling microsporidiosis. This study analyzed the transcriptome of muscle tissues from two crab species at different stages of Ameson portunus infection, revealing differential molecular responses and dynamic host responses. The findings suggested that A. portunus can regulate host cellular responses to achieve proliferation, and the infection causes DNA damage in host cells. Metabolic function and immune responses were significantly affected in severely infected crabs, leading to reduced food intake and slow growth. The two crab species exhibited different immune responses to A. portunus infection.
Article
Fisheries
Zhanning Xu, Yujie Wei, Huiyang Huang, Songlin Guo, Haihui Ye
Summary: The study investigated the role of sNPF in the immune response of the mud crab Scylla paramamosain. It found that sNPF mediates hemocyte phagocytosis through the sNPF-R receptor-coupled AC-cAMP-PKA pathway, influencing innate immune processes in the crab.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Environmental Sciences
Guoling Liang, Kangxiang Qin, Yuhao Chen, Mingming Niu, Huan Wang, Chunlin Wang, Changkao Mu, Lizhi Chen, Fangfang Wang, Qiong Su, Ritong Zhu
Summary: This study investigated the transcriptomic changes of Scylla paramamosain in different salinity environments and found that metabolic processes and cell membrane catalysis play crucial roles in osmoregulation. The adaptation to inland low salinity saline-alkaline water is dependent on cations such as Ca2+, Na+, and K+ as well as the regulation of cAMP signaling pathway.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Fisheries
Yongsheng Zhang, Ting Liu, Qian Sun, Ming Zhang, Ngoc Tuan Tran, Xiuli Chen, Shengkang Li
Summary: This study reveals the involvement of exosomal protein SpFlotillin-1 in regulating immune responses during bacterial infection in mud crabs. Up-regulation of SpFlotillin-1 inhibits phagocytosis and reduces antimicrobial peptide expression, while increasing ROS production. Moreover, SpFlotillin-1 densely packaged in exosomes modulates phagocytosis, AMP expression, and ROS production through the MAPK and NF-kappa B pathways, ultimately activating the immune response to bacterial infection in mud crabs.
Article
Fisheries
Hang Hu, Xinshan Zhao, Shengkang Li, Yi Gong
Summary: The study identified SpICAD in mud crabs as having an immunoregulatory role during WSSV infection by inhibiting apoptosis, ultimately promoting virus replication. These findings offer new insights into the anti-viral immune response of mud crabs.
Article
Biochemistry & Molecular Biology
Ya-Qiu Zhang, Yi-Qin Deng, Juan Feng, Jian-Mei Hu, Hao-Xiang Chen, Zhi-Xun Guo, You-Lu Su
Summary: Vibrio harveyi, a harmful bacterium to the aquaculture industry, was studied to investigate the role of LuxS gene. The results showed that LuxS plays an important role in controlling motility, flagella biogenesis, and extracellular protease secretion in V. harveyi.
CANADIAN JOURNAL OF MICROBIOLOGY
(2022)
Article
Fisheries
Yu-Kun Jie, Zhi-Ping Luo, Jia-Wei Xie, Chang-Hong Cheng, Hong-Ling Ma, Guang-Xin Liu, Jian-Jun Jiang, Yi-Qin Deng, Juan Feng, Zhi-Xun Guo
Summary: In this study, a PFK gene from the mud crab Scylla paramamosain, named SpPFK, was cloned and characterized. Knocking down SpPFK using RNA interference significantly reduced lactate dehydrogenase expression after MCDV-1 infection and increased the survival rate of mud crabs. These results suggest that SpPFK plays an important role in the proliferation of MCDV-1 in mud crabs.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Environmental Sciences
Yiqin Deng, Can Mao, Ziyang Lin, Wenxiao Su, Changhong Cheng, Yong Li, Qunhong Gu, Ren Gao, Youlu Su, Juan Feng
Summary: This study investigated the pollution of antibiotic resistance genes (ARGs) in sea bass ponds and its relationship with bacterial community composition and environmental parameters. The results showed that sea bass ponds are reservoirs of ARGs, and the changes are mainly influenced by water temperature, dissolved oxygen, and NO3-. Regulation of these environmental factors can reduce drug resistance risk in aquaculture ponds.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Engineering, Electrical & Electronic
Xianhao Chen, Guangyu Zhu, Yiqin Deng, Yuguang Fang
Summary: This paper investigates the problem of federated learning over wireless mesh networks and proposes a framework for in-network model aggregation to reduce outgoing data traffic and improve model aggregation under limited communication resources. The optimization problem is formulated by considering model aggregation, routing, and spectrum allocation, and a solution approach based on mixed-integer linear programming is developed. Simulation results demonstrate the effectiveness of the proposed solution and the superiority of the in-network aggregation scheme.
IEEE TRANSACTIONS ON WIRELESS COMMUNICATIONS
(2022)
Article
Fisheries
Hongling Ma, Changhong Cheng, Yiqin Deng, Guangxin Liu, Sigang Fan, Juan Feng, Zhixun Guo
Summary: In this study, vaccines against Ranavirus-type grouper iridovirus (GIV-R) and/or Vibrio harveyi were developed and evaluated. The results showed that these vaccines provided effective protection against both pathogens. Additionally, booster immunization prolonged the duration of protection against V. harveyi infection.
Article
Fisheries
Chang-Hong Cheng, Guang-Xin Liu, Yi-Qin Deng, Juan Feng, Jian-Jun Jiang, Zhi-Xun Guo
Summary: In this study, a novel full-length of Glutaredoxin 5 (Sp-Grx 5) was cloned and characterized in the mud crab. Sp-Grx 5 was widely expressed in various tissues with high-level expression in hepatopancreas. It was found to be involved in innate immunity and detoxification, playing important roles in redox homeostasis and innate immune response in crustaceans.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Engineering, Electrical & Electronic
Bo Li, Xiaoheng Deng, Xuechen Chen, Yiqin Deng, Jian Yin
Summary: This paper focuses on controller placement problem in Internet of Vehicles (IoV) using software-defined networks (SDN) and mobile-edge computing (MEC). A multi-objective optimization problem is investigated, and an algorithm based on multi-agent deep Q-learning networks (MADQN) is developed to efficiently solve the problem. The algorithm is accelerated using multi-process technology.
IEEE TRANSACTIONS ON VEHICULAR TECHNOLOGY
(2022)
Article
Engineering, Electrical & Electronic
Yiqin Deng, Zhigang Chen, Xianhao Chen, Yuguang Fang
Summary: As the demand for multi-access edge computing (MEC) increases exponentially, resource limitations at individual edge servers (ESs) become bottlenecks. This paper presents a cooperative MEC framework that enables network-wide cooperation of ESs through multi-hop task relaying. The framework balances the workload for both spot communication and computing, maximizing overall throughput. An optimization problem is formulated and matching theory is applied to find a low-complexity task offloading scheme. Numerical analysis demonstrates the effectiveness of the proposed scheme and the superiority of the multi-hop relay-assisted MEC systems.
IEEE TRANSACTIONS ON VEHICULAR TECHNOLOGY
(2023)
Article
Environmental Sciences
Yiqin Deng, Jianjun Jiang, Yinbang Huang, Changhong Cheng, Ziyang Lin, Guangxin Liu, Zhixun Guo, Juan Feng
Summary: The concentration of dissolved oxygen affects the spread of antibiotic resistance genes (ARGs). Low oxygen treatment increases the accumulation of ammonium and nitrite and the abundance of ARGs and mobile genetic elements (MGEs), especially ARGs resistant to drugs such as sulfonamide, chloramphenicol, and macrolides. This study highlights the importance of oxygen concentration in controlling the spread of ARGs, especially through horizontal gene transfer.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Wenxiao Su, Yiqin Deng, Shujun Zang, Si'ao Gao, Juan Feng
Summary: Leucine-responsive regulatory protein (Lrp) plays a negative regulatory role in Vibrio alginolyticus, reducing its virulence by decreasing swarming motility, biofilm formation, extracellular protease secretion activity, and tolerance to copper ions. The expression of Lrp is influenced by various physicochemical factors.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Fisheries
Chang-Hong Cheng, Shu-Fei Zhang, Hong-Ling Ma, Guang-Xin Liu, Si-Gang Fan, Yi-Qin Deng, Jian-Jun Jiang, Juan Feng, Zhi-Xun Guo
Summary: A novel cytochrome P450 enzyme, Sp-CYP2, was cloned and characterized in the mud crab. Sp-CYP2 was found to be widely expressed in various tissues, with the highest expression in the heart and hepatopancreas. Its expression was induced by ammonia exposure and infection of Vibrio parahaemolyticus. Knocking down Sp-CYP2 increased oxidative stress and mortality in mud crabs, suggesting its crucial role in defense against environmental stress and pathogen infection in crustaceans.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Environmental Sciences
Yiqin Deng, Si'ao Gao, Liwen Xu, Changhong Cheng, Hongling Ma, Juan Feng
Summary: This study systematically analyzed the effect of environmental stress on the conjugation ability of the marine fish pathogen Vibrio harveyi. The results showed that certain stress exposure can induce the fertility of V. harveyi and promote the transfer of shuttle plasmids from Escherichia coli. Ethanol and heat stress exhibited the strongest induction effect. These findings will contribute to the development of gene knockout technology and advance the molecular theoretical research on V. harveyi, as well as support the establishment of disease prevention and control strategies based on environmental regulation of gene transfer.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Environmental Sciences
Ziyang Lin, Yiqin Deng, Wenxiao Su, Qian Wang, Haoxiang Chen, Yun Sun, Juan Feng
Summary: This study investigated the abundance and distribution of bacterial antibiotic resistance associated with farmed fish in Zhuhai, China. The results showed high resistance to erythromycin and sulfamethoxazole with trimethoprim, while resistance to other antibiotics was generally low. Acinetobacter sp. and Pseudomonas sp. were the dominant antibiotic resistant bacteria, and most cultivable resistant bacteria were opportunistic pathogens. The abundance of sul family genes was higher than other tested resistance genes, and the presence of antibiotics in the water positively correlated with antibiotic resistance genes and bacteria.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Computer Science, Hardware & Architecture
Xianhao Chen, Yiqin Deng, Guangyu Zhu, Danxin Wang, Yuguang Fang
Summary: This article discusses the concept of end-to-end (E2E) service auction in 5G and beyond, where emerging services like edge computing/intelligence demand heterogeneous CCS resources. By addressing the joint problem of network optimization and auction design, this auction model can provide E2E QoS guarantees while ensuring economic properties.
IEEE WIRELESS COMMUNICATIONS
(2022)
Article
Computer Science, Hardware & Architecture
Yiqin Deng, Xianhao Chen, Guangyu Zhu, Yuguang Fang, Zhigang Chen, Xiaoheng Deng
Summary: This article introduces the basic concepts, performance metrics, killer applications, architectural design, modeling approaches and future research directions of multi-access edge computing (MEC). It is a useful quick introduction for beginning researchers.
IEEE WIRELESS COMMUNICATIONS
(2022)
