Article
Cell Biology
Amadeus Xu, Angika Basant, Sibylle Schleich, Timothy P. Newsome, Michael Way
Summary: The study reveals that intracellular mature viruses (IMVs) and intracellular enveloped viruses (IEVs) can undergo microtubule-based motility and recruit the kinesin-1 motor protein for transport. IMVs are the first and most abundant infectious form of vaccinia virus during its replication cycle. These findings provide insights into the transport mechanism of the virus.
JOURNAL OF CELL SCIENCE
(2023)
Article
Cell Biology
Mia J. Konjikusic, Chanjae Lee, Yang Yue, Bikram D. Shrestha, Ange M. Nguimtsop, Amjad Horani, Steven Brody, Vivek N. Prakash, Ryan S. Gray, Kristen J. Verhey, John B. Wallingford
Summary: Most motile cilia have a stereotyped structure, but the mechanism of Kif9/Klp1 function remains poorly defined. However, our study showed that Kif9 is necessary for ciliary motility and the proper localization of various proteins. Additionally, single-molecule assays revealed that Xenopus Kif9 is a long-range processive motor.
JOURNAL OF CELL SCIENCE
(2023)
Article
Cell Biology
Song Yang, Youguang Luo, Mulin Yang, Hua Ni, Hanxiao Yin, Ming Hu, Min Liu, Jun Zhou, Yunfan Yang, Dengwen Li
Summary: Src is a key player in cell division, migration, adhesion, and survival. Its overactivation in tumors promotes cancer cell survival and mitosis. Src is conserved across species and its inhibition leads to abnormal expression of chromosomal passenger complex components, resulting in multipolar spindle formation and cell division failure. The findings suggest that Src inhibitors can be used as a novel approach for cancer treatment.
CELL AND TISSUE RESEARCH
(2023)
Article
Cell Biology
Drisya Vijayakumari, Janina Mueller, Silke Hauf
Summary: The study shows that Cdc48 (p97/VCP) protein is not important in mitosis. Low levels of separase in the cdc48-353 mutant are not dependent on mitosis and there is no evidence of enhanced separase degradation. The results highlight the importance of understanding the synthesis of separase for further research.
Article
Multidisciplinary Sciences
Shinichiro Komaki, Eelco C. Tromer, Geert De Jaeger, Nancy De Winne, Maren Heese, Arp Schnittger
Summary: A study identified BORI1 and BORI2 as Survivin-like proteins in plants, which are essential for proper development. These proteins bind to phosphorylated histone H3, facilitating the association of CPC with chromatin. The presence of a helical domain that promotes complex formation with other scaffold components is a shared feature of Survivin-type proteins in animals and plants, and the addition of a phosphate-binding domain evolved independently in different eukaryotic groups.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Puja Chakraborty, Ratnadip Paul, Abhishek Chowdhury, Nayonika Mukherjee, Somsubhra Nath, Susanta Roychoudhury
Summary: This study investigates the role of histone ubiquitylation in transcription regulation, particularly the mono-ubiquitylation of histone 2B by RING finger motif-containing ubiquitin ligases. The study shows that the anaphase-promoting complex/cyclosome (APC/C), along with its adapter protein Cdc20, catalyses the mono-ubiquitylation of Lysine-120 on the UBCH10 promoter. The study also uncovers a cell-cycle-specific pattern of this modification and suggests a crosstalk between acetylation and ubiquitylation in UBCH10 trans-regulation.
Article
Cell Biology
Delaney Sherwin, Emily Gutierrez-Morton, Michael Bokros, Cory Haluska, Yanchang Wang
Summary: The conserved chromosomal passenger complex (CPC) corrects kinetochore attachment errors and prevents checkpoint silencing. Sli15 is phosphorylated by both CDK and Ipl1 kinase, and Cdc14 phosphatase reverses CDK-induced phosphorylation to promote CPC translocation. In addition, Fin1-PP1 also dephosphorylates Sli15 to enable CPC translocation. This pathway is crucial for accurate chromosome segregation.
MOLECULAR BIOLOGY OF THE CELL
(2023)
Article
Biochemistry & Molecular Biology
Lisa Watson, Tanya N. Soliman, Khalil Davis, Joanna Kelly, Nicola Lockwood, Xiaoping Yang, Steven Lynham, John D. Scott, Victoria Crossland, Neil Q. McDonald, David J. Mann, Alan Armstrong, Ulrike Eggert, Peter J. Parker
Summary: The study elucidates the role of PKC-ε in exiting from the abscission checkpoint. Assembly of the 14-3-3 complex and phosphorylation of Aurora B S227 are directly linked to the activity-dependent enrichment of PKC-ε at the midbody, with 14-3-3 association shown to be unnecessary for PKC-ε control.
BIOCHEMICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Maike Nagel, Marvin Noss, Jishu Xu, Nicola Horn, Marius Ueffing, Karsten Boldt, Rebecca Schuele
Summary: Neurons rely on regulated RNA localization and gene expression for their proper functioning. Mutations in the KIF1C gene lead to abnormal transportation of RNA in neurons and contribute to degeneration of motoneurons. KIF1C was found to interact with the exon junction complex (EJC) and the interaction is mediated by RNA. These findings highlight the importance of KIF1C in mRNA localization.
Article
Biology
Zhihui Zhu, Mohammad Bani Ismail, Miki Shinohara, Akira Shinohara
Summary: Depletion of Cdc53 leads to defects in synaptonemal complex formation during meiosis while maintaining crossover formation, suggesting the uncoupling of meiotic recombination with synaptonemal complex assembly. The collaboration between Pch2 and Cdc53 is critical for synaptonemal complex assembly and meiotic recombination.
LIFE SCIENCE ALLIANCE
(2021)
Article
Multidisciplinary Sciences
Ringaile Zaksauskaite, Ruth C. Thomas, Freek van Eeden, Sherif F. El-Khamisy
Summary: Deficiency in the DNA end-processing enzyme, tyrosyl-DNA phosphodiesterase 1 (TDP1), causes progressive neurodegeneration in humans. However, the tdp1(-/-) zebrafish embryo does not require TDP1 to protect from Top1-DNA damage, instead relying on apex2 and ercc4 (xpf) as putative players fulfilling this role. This highlights the need for distinct DNA repair factors across the life span of vertebrates.
Article
Multidisciplinary Sciences
Mario Pinar, Ana Alonso, Vivian de los Rios, Ignacio Bravo-Plaza, Alvaro de la Gandara, Antonio Galindo, Ernesto Arias-Palomo, Miguel A. Penalva
Summary: In the RAB11 exocytic pathway of Aspergillus nidulans, secretory vesicles are transported to the hyphal tip by kinesin-1/KinA and transferred to myosin MyoE. MyoE concentrates the vesicles at an apical store, resembling the presynaptic active zone. The interaction network involving RAB11 and HUM components recruits UDS1 and HMSV to form a stable HUM complex with MyoE. Ablating UDS1 or HMSV impairs transport of vesicles to the apex.
Article
Multidisciplinary Sciences
Maria Chatzifrangkeskou, Paris A. Skourides
Summary: This study investigates the distribution of the Ciliary Adhesion (CA) complex on cilia and its interactions with the microtubule network using super-resolution imaging. The findings show that the apical CA complex forms at the distal end of the basal foot and relies on microtubules. These results suggest that CA may have additional roles in regulating the organization of the microtubule network in multiciliated cells.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Sang Hyup Lee, Ji-Hye Yang, Ui-Hyun Park, Hanbyeul Choi, Yoo Sung Kim, Bo-Eun Yoon, Hye-Jeong Han, Hyun-Taek Kim, Soo-Jong Um, Eun-Joo Kim
Summary: SIRT1, a NAD-dependent deacetylase, is downregulated by ubiquitin-mediated proteasomal degradation during stress-induced premature senescence (SIPS). Two proteins, Cdh1 and AROS, regulate the stability of SIRT1 in opposite ways and may be potential targets for treating pulmonary fibrosis.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
Article
Multidisciplinary Sciences
Thomas D. Jackson, Jordan J. Crameri, Linden Muellner-Wong, Ann E. Frazier, Catherine S. Palmer, Luke E. Formosa, Daniella H. Hock, Kenji M. Fujihara, Tegan Stait, Alice J. Sharpe, David R. Thorburn, Michael T. Ryan, David A. Stroud, Diana Stojanovski
Summary: Sideroflexins (SFXNs) are a family of proteins that have different functions in mitochondrial biology. Loss of SFXN4 leads to complex I assembly defect and it interacts with the core components of the mitochondrial complex I intermediate assembly complex.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Cell Biology
Amol Aher, Anna Akhmanova
CURRENT OPINION IN CELL BIOLOGY
(2018)
Article
Cell Biology
Amol Aher, Maurits Kok, Ashwani Sharma, Ankit Rai, Natacha Olieric, Ruddi Rodriguez-Garcia, Eugene A. Katrukha, Tobias Weinert, Vincent Olieric, Lukas C. Kapitein, Michel O. Steinmetz, Marileen Dogterom, Anna Akhmanova
DEVELOPMENTAL CELL
(2018)
Article
Cell Biology
Gaurav Barve, Shreyas Sridhar, Amol Aher, Mayurbhai H. Sahani, Sarika Chinchwadkar, Sunaina Singh, K. N. Lakshmeesha, Michael A. McMurray, Ravi Manjithaya
JOURNAL OF CELL SCIENCE
(2018)
Article
Cell Biology
Ingrid E. Adriaans, Angika Basant, Bas Ponsioen, Michael Glotzer, Susanne M. A. Lens
JOURNAL OF CELL BIOLOGY
(2019)
Article
Cell Biology
Peter Jan Hooikaas, Maud Martin, Tobias Muehlethaler, Gert-Jan Kuijntjes, Cathelijn A. E. Peeters, Eugene A. Katrukha, Luca Ferrari, Riccardo Stucchi, Daan G. F. Verhagen, Wilhelmina E. van Rie, Ilya Grigorievie, A. F. Maarten Altelaar, Casper C. Hoogenraad, Stefan G. D. Ruediger, Lukas C. Kapiteini, Anna Akhmanova
JOURNAL OF CELL BIOLOGY
(2019)
Article
Cell Biology
Xingxiu Pan, Yujie Cao, Riccardo Stucchi, Peter Jan Hooikaas, Sybren Portegies, Lena Will, Maud Martin, Anna Akhmanova, Martin Harterink, Casper C. Hoogenraad
Article
Neurosciences
Amelie Freal, Dipti Rai, Roderick P. Tas, Xingxiu Pan, Eugene A. Katrukha, Dieudonnee van de Willige, Riccardo Stucchi, Amol Aher, Chao Yang, A. F. Maarten Altelaar, Karin Vocking, Jan Andries Post, Martin Harterink, Lukas C. Kapitein, Anna Akhmanova, Casper C. Hoogenraad
Article
Biochemistry & Molecular Biology
Ruddi Rodriguez-Garcia, Vladimir A. Volkov, Chiung-Yi Chen, Eugene A. Katrukha, Natacha Olieric, Amol Aher, Ilya Grigoriev, Magdalena Preciado Lopez, Michel O. Steinmetz, Lukas C. Kapitein, Gijsje Koenderink, Marileen Dogterom, Anna Akhmanova
Article
Multidisciplinary Sciences
Yanzhang Luo, ShengQi Xiang, Peter Jan Hooikaas, Laura van Bezouwen, A. S. Jijumon, Carsten Janke, Friedrich Forster, Anna Akhmanova, Marc Baldus
NATURE COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Amol Aher, Dipti Rai, Laura Schaedel, Jeremie Gaillard, Karin John, Qingyang Liu, Maarten Altelaar, Laurent Blanchoin, Manuel Thery, Anna Akhmanova
Article
Biology
Peter Jan Hooikaas, Hugo G. J. Damstra, Oane J. Gros, Wilhelmina E. van Riel, Maud Martin, Yesper T. H. Smits, Jorg van Loosdregt, Lukas C. Kapitein, Florian Berger, Anna Akhmanova
Article
Biology
Andrea Serra-Marques, Maud Martin, Eugene A. Katrukha, Ilya Grigoriev, Cathelijn A. E. Peeters, Qingyang Liu, Peter Jan Hooikaas, Yao Yao, Veronika Solianova, Ihor Smal, Lotte B. Pedersen, Erik Meijering, Lukas C. Kapitein, Anna Akhmanova