Article
Biochemistry & Molecular Biology
William Dudefoi, Hanitra Rabesona, Camille Rivard, Muriel Mercier-Bonin, Bernard Humbert, Helene Terrisse, Marie-Helene Ropers
Summary: The study found that two types of food grade TiO2 samples and one nano-sized TiO2 sample could form large agglomerates in digestive fluids, but had limited impact on the overall digestion of carbohydrates and proteins. Under salivary conditions, TiO2 had an inhibitory effect on the enzymatic activity of alpha-amylase, which was reduced to almost 10% in the intestinal fluid.
Article
Biophysics
Loredana F. Leopold, Cristina Coman, Doina Clapa, Ioana Oprea, Alexandra Toma, Stefania D. Iancu, Lucian Barbu-Tudoran, Maria Suciu, Alexandra Ciorita, Adrian I. Cadis, Laura Elena Muresan, Ioana Mihaela Perhaita, Lucian Copolovici, Dana M. Copolovici, Florina Copaciu, Nicolae Leopold, Dan C. Vodnar, Vasile Coman
Summary: This study evaluated the effects of large-scale TiO2 and ZnO nanoparticles on in vitro-grown soybean plants. The results showed that ZnO nanoparticles exhibited higher toxicity compared to TiO2, leading to negative effects on plant growth and metabolic processes.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Oscar Ramiro Andrade, Rafael Camarillo, Fabiola Martinez, Carlos Jimenez, Jesusa Rincon
Summary: The synthesis and characterization of TiO2 nanoparticles in supercritical CO2 medium from different precursors and with different hydrolytic agent/precursor ratios were studied. The physicochemical properties of the catalysts were not significantly affected by these variables, but the catalytic performances showed some differences. Photocatalysts obtained from certain precursors at higher hydrolytic agent/precursor ratios showed higher CO2 photoconversions, attributed to their appropriate crystal size, surface area, light absorption, and charge transfer properties.
Article
Chemistry, Multidisciplinary
Isabela A. P. Farias, Carlos C. L. Santos, Aline L. Xavier, Tatianne M. Batista, Yuri M. Nascimento, Jocianelle M. F. F. Nunes, Patricia M. F. Silva, Raimundo A. Menezes-Junior, Jailson M. Ferreira, Edeltrudes O. Lima, Josean F. Tavares, Marianna Sobral, Dawy Keyson, Fabio C. Sampaio
Summary: Fungal infection poses a public health concern with antifungal resistance being common in Candida albicans. Cerium oxide nanoparticles showed promising antifungal activity against Candida albicans strains without significant antioxidant effects. Acute toxicity studies in mice and brine shrimp did not reveal harmful effects, supporting the potential use of CeNP as an antifungal agent in biotechnology and biomedical applications.
ARABIAN JOURNAL OF CHEMISTRY
(2021)
Review
Biotechnology & Applied Microbiology
Poonam Sharma, Rekha Kumari, Meena Yadav, Rup Lal
Summary: This review evaluates the impact of different physicochemical parameters and experimental conditions of titanium dioxide nanoparticles (TiO(2)NPs) on their toxicity towards bacteria. The study highlights the lack of standardization and the importance of considering differences between in vivo and in vitro systems in toxicological assessments.
INDIAN JOURNAL OF MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Natalia Janik-Olchawa, Agnieszka Drozdz, Damian Ryszawy, Maciej Pudelek, Karolina Planeta, Zuzanna Setkowicz, Maciej Sniegocki, Magdalena Wytrwal-Sarna, Marta Gajewska, Joanna Chwiej
Summary: This study compared the impact of PEG-coated magnetite nanoparticles with different core sizes on six selected cell lines. It was found that exposure to these nanoparticles led to decreased cell viability, which was dependent on exposure time rather than core diameter. The nanoparticles not only increased the proliferation rate of macrophages but also stimulated tumor cell divisions. Additionally, the smallest nanoparticles mainly induced an increase in ROS levels in cells.
SCIENTIFIC REPORTS
(2021)
Article
Plant Sciences
Nuno Mariz-Ponte, Celeste M. Dias, Artur M. S. Silva, Conceicao Santos, Sonia Silva
Summary: The study shows the potential of low doses of nTiO(2) in alleviating metal phytotoxicity, especially that of lead. The addition of nTiO(2) can mitigate the harmful effects of lead and aluminum on plants, increasing pigment content and photosynthetic efficiency.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Takayuki Ishizaka, Maya Chatterjee, Hajime Kawanami
Summary: PI fine particles encapsulating a large number of TiO2 nanoparticles were successfully fabricated using the emulsion re-precipitation method in a multistep flow synthetic system. The fabricated material had a spherical structure with TiO2 nanoparticles disproportionately embedded near the surface, showing high UV shielding efficiency according to UV-vis transmission spectra.
Article
Biochemistry & Molecular Biology
Jeremy A. Nestele, Anne-Katrin Rohlfing, Valerie Dicenta, Alexander Bild, Daniela Eissler, Frederic Emschermann, Marcel Kremser, Konstantin Krutzke, Tilman E. Schaeffer, Oliver Borst, Moran Levi, Netanel Korin, Meinrad Paul Gawaz
Summary: Nanoparticles provide a novel approach for targeted antithrombotic therapy, delivering drugs to specific sites with low side effects. In this study, nanoparticles combined with established proteins successfully inhibited platelet aggregation and thrombus formation, indicating a promising therapeutic strategy for antithrombotic treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Environmental
Liwen Qiang, Nan Zhao, Kaizhen Liao, Xichao Sun, Qiang Wang, Hangbiao Jin
Summary: ENPs can resist heavy metal toxicity in plants, but their coexposure still exhibits toxicity to plants. This study aimed to investigate the toxic mechanism of nano TiO2 or TiO2-Cd on rice using transcriptomics and metabolomics. The findings showed that nano TiO2-Cd coexposure significantly decreased the plant height and root dry weight of rice. Nano TiO2 exposure induced regulation of DEGs and metabolites, while nano TiO2-Cd coexposure identified a greater number of DEGs and differential metabolites. The study highlights the importance of being cautious when using ENPs to combat heavy metal phytotoxicity.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Polymer Science
Tatsiana V. Shakola, Vasili V. Rubanik, Vasili V. Rubanik Jr, Aleh V. Kurliuk, Anatoly A. Kirichuk, Alexander G. Tskhovrebov, Anton R. Egorov, Andreii S. Kritchenkov
Summary: This study focused on synthesizing and evaluating chitosan-based antibacterial polymers and nanoparticles with benzothiazole substituents. In vivo tests on white rats showed significant antibacterial activity and low toxicity of the synthesized derivatives. The findings highlight the potential of these chitosan-based systems as effective alternatives to traditional antibiotics.
Review
Chemistry, Multidisciplinary
Ehsan Nazarzadeh Zare, Xuanqi Zheng, Pooyan Makvandi, Homa Gheybi, Rossella Sartorius, Cynthia K. Y. Yiu, Mohsen Adeli, Aimin Wu, Ali Zarrabi, Rajender S. Varma, Franklin R. Tay
Summary: Metal-based nanoentities are widely used in industrial and biomedical fields, with their biological impacts influenced by factors such as size, shape, and composition. The size and shape of metallic nanostructures affect cell viability, while nonspherical metal-based nanoarchitectures have anisotropic properties that make them exciting candidates for biomedical applications.
Article
Environmental Sciences
Ozge Kose, David Beal, Sylvie Motellier, Nathalie Pelissier, Veronique Collin-Faure, Magda Blosi, Rossella Bengalli, Anna Costa, Irini Furxhi, Paride Mantecca, Marie Carriere
Summary: The toxicity of silver nanoparticles (Ag NPs) in human intestinal cells was investigated in this study. The NPs were either uncoated or coated with polyvinylpyrrolidone (Ag PVP) or hydroxyethylcellulose (Ag HEC) and digested in simulated gastrointestinal fluids. Physicochemical transformations during digestion were analyzed before assessing toxicity. The evaluation strategy was based on adverse outcome pathways (AOPs) and included assessing cytotoxicity, oxidative stress, genotoxicity, cell cycle perturbation, and apoptosis. Ag NPs exhibited concentration-dependent toxicity, causing cell death, oxidative stress, DNA damage, and cell cycle disruption. In vitro digestion did not significantly affect their toxicity, except for genotoxicity. These findings suggest potential toxicity of ingested Ag NPs, which varied with coating but was not altered by digestion.
Article
Nanoscience & Nanotechnology
Barbara Wojcik, Katarzyna Zawadzka, Slawomir Jaworski, Marta Kutwin, Malwina Sosnowska, Agnieszka Ostrowska, Marta Grodzik, Artur Malolepszy, Marta Mazurkiewicz-Pawlicka, Mateusz Wierzbicki
Summary: This study evaluated the cytotoxicity of 13 types of diamond nanoparticles with different production methods, sizes, and surface functional groups on tumor cell lines and a non-tumor cell line. The results showed that diamond nanoparticles had no toxicity on non-tumor cells but moderate toxicity on glioblastoma cell lines. The production method did not affect the toxicity, but modifications of the nanoparticles had varying effects on cell response.
Article
Toxicology
Anna Mittag, Alina Singer, Christian Hoera, Martin Westermann, Alexander Kaempfe, Michael Glei
Summary: This study investigates the fate and impact of two different sized ZnO NP during in vitro digestion on model systems of the intestinal barrier. The results show that digested ZnO NP interact with the cells of an intact intestinal barrier without causing serious cell damage.
PARTICLE AND FIBRE TOXICOLOGY
(2022)
Article
Environmental Sciences
Mohamed-Nour Azzougagh, Francois-Xavier Keller, Mehmet Cici, Elodie Cabrol, Jeremie Pourchez
Summary: This research investigates ventilation and aerosol concentration in a metal additive manufacturing facility using L-PBF machine. Real-time metrology instruments were used to assess particle number concentration, revealing the impact of ventilation and human activity on particle levels. The study highlights the importance of individual protective equipment during maintenance and cleaning operations.
TOXICOLOGY AND ENVIRONMENTAL HEALTH SCIENCES
(2022)
Article
Environmental Sciences
Helene Niculita-Hirzel, Audrey S. Vanhove, Lara Leclerc, Francoise Girardot, Jeremie Pourchez, Severine Allegra
Summary: The increase in legionellosis incidence in the general population has raised concerns about the sources of infection, including showering. This study assessed the rate of aerosolization of Legionella pneumophila, a water pathogen, during showering with different shower systems. The results showed that the risk of exposure to viable Legionella is not expected to significantly increase with the use of water-efficient showerheads.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Letter
Critical Care Medicine
Jeremie Pourchez, Clement Mercier
LANCET RESPIRATORY MEDICINE
(2022)
Article
Chemistry, Medicinal
Etienne Bourgart, Lara Leclerc, Jeremie Pourchez, Mohamad Sleiman
Summary: This research aims to evaluate methods for measuring nicotine forms in nicotine salts and study the protonation of nicotine in aerosols post vaporization. The results show that nicotine forms are consistent between e-liquids and aerosols, indicating that e-liquids can be used as surrogates for aerosols. pH measurement is a commonly used method, while liquid-liquid extraction (LLE) has the highest variability and is the most time-consuming. Calculation of alpha(fb) from molar ratio is the most robust and versatile method.
CHEMICAL RESEARCH IN TOXICOLOGY
(2022)
Editorial Material
Respiratory System
V. Forest, J. Pourchez
Article
Chemistry, Medicinal
Martin Walter, Walter D. C. Schenkeveld, Maura Tomatis, Karin Schelch, Barbara Peter-Voroesmarty, Gerald Geroldinger, Lars Gille, Maria C. Bruzzoniti, Francesco Turci, Stephan M. Kraemer, Michael Grusch
Summary: Chrysotile asbestos is a carcinogenic mineral that contains reactive iron surface sites. When oxidized by H2O2, chromium readily dissolves from the fibers and accumulates in lung tissues and cancer cells. However, unlike iron, chromium does not significantly contribute to the radical production of chrysotile.
CHEMICAL RESEARCH IN TOXICOLOGY
(2022)
Article
Environmental Sciences
Elena Gazzano, Jasmine Rita Petriglieri, Elisabetta Aldieri, Bice Fubini, Christine Laporte-Magoni, Cristina Pavan, Maura Tomatis, Francesco Turci
Summary: Exposure to asbestos and asbestos-like minerals is associated with severe lung diseases. The study investigated the toxicity and cellular effects of fibrous antigorites on murine macrophages and alveolar epithelial cells. The findings revealed significant cellular toxicity of fibrous antigorites, which correlated with their fibrous habit and surface reactivity.
ENVIRONMENTAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Chiara Bellomo, Cristina Pavan, Gianluca Fiore, Guillermo Escolano-Casado, Lorenzo Mino, Francesco Turci
Summary: Occupational exposure to quartz dust is associated with fatal diseases. The contribution of nanometric particles to the toxicity of quartz is still largely unknown. In this study, a top-down milling procedure was used to obtain a nanometric quartz with similar surface properties to fractured quartz, providing a novel material for toxicological investigations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Caroline Marie-Desvergne, Muriel Dubosson, Lara Leclerc, Catherine Campo, Dimitrios Bitounis, Valerie Forest, Jeremie Pourchez, Michele Cottier, Jean-Michel Vergnon, Adeline Tarantini, Veronique Chamel-Mossuz
Summary: In the field of biomonitoring, exhaled breath condensate (EBC) is a potential matrix for assessing inhalation exposure biomarkers non-invasively. This study compared the particulate content and elemental composition of EBC with bronchial washes (BWs) and bronchoalveolar lavages (BALs) in patients with interstitial lung diseases (ILDs). EBC was found to represent the sub-micron and nano-sized particle fractions of BAL and BW, with lower overall levels of elements. Silicon (Si) was the main component in all respiratory matrices, and levels of Si in EBC were elevated in ILD patients compared to healthy subjects. The particulate content of EBC is associated with lung particle burden and may correlate with pathologies, making it a relevant biomonitoring technique in occupational and clinical fields.
JOURNAL OF BREATH RESEARCH
(2023)
Article
Chemistry, Medicinal
Lara Leclerc, Nathalie Prevot, Sophie Hodin, Xavier Delavenne, Heribert Mentzel, Uwe Schuschnig, Jeremie Pourchez
Summary: This study evaluated the impact of nasal delivery technique and nebulizing technologies on the targeting of maxillary sinuses with acoustic aerosol. Different devices and techniques were assessed, and the results showed that a closed soft palate and the PARI SINUS nebulizer yielded higher intrasinus aerosol deposition compared to other conditions. The choice of nebulizing technology and nasal delivery technique are crucial factors in improving aerosol deposition in the maxillary sinuses.
Review
Biochemistry & Molecular Biology
Cristina Pavan, Rosangela Santalucia, Guillermo Escolano-Casado, Piero Ugliengo, Lorenzo Mino, Francesco Turci
Summary: The study focuses on the role of hydroxyls (OH) on oxide particles (OxPs) in molecular initiating events leading to OxPs toxicity. The analytical methods used to characterize surface OH, including thermogravimetry, titration, zeta potential measurements, and spectroscopic approaches (NMR, XPS), are discussed. The importance of modelling techniques (MD, DFT) for describing the interactions between membranes/proteins and OxPs surfaces is also highlighted. A new approach methodology (NAM) based on IR spectroscopy and bioanalytical assays is proposed for investigating the molecular interactions of OxPs with biomolecules and membranes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Riccardo Leinardi, Jasmine Rita Petriglieri, Amandine Pochet, Yousof Yakoub, Marie Lelong, Alain Lescoat, Francesco Turci, Valerie Lecureur, Francois Huaux
Summary: Exposing macrophages to short or long asbestos fibers revealed that short fibers induced pyroptotic-related immunogenic cell death, while long fibers triggered apoptosis. The effects of short fibers were mainly mediated by TLR4, whereas the response to long fibers involved multiple factors. These findings suggest that the size and surface features of asbestos fibers play a significant role in modulating cell death and inflammatory pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Chiara Bellomo, Valeria Lagostina, Cristina Pavan, Maria Cristina Paganini, Francesco Turci
Summary: Industrial processing of quartz produces toxic dust, as fracturing quartz crystals leads to the formation of highly reactive surface species and silanol, damaging biological membranes. The formation and reactivity of quartz radicals in different molecular environments were evaluated, and their membranolytic activity was measured through in vitro hemolysis test.
