Article
Agronomy
Pedro Revilla, Ana Butron, Victor Manuel Rodriguez, Renaud Rincent, Alain Charcosset, Catherine Giauffret, Albrecht E. Melchinger, Chris-Carolin Schoen, Eva Bauer, Thomas Altmann, Dominique Brunel, Jesus Moreno-Gonzalez, Laura Campo, Milena Ouzunova, Angel Alvarez, Jose Ignacio Ruiz de Galarreta, Jacques Laborde, Rosa Ana Malvar
Summary: Cold tolerance in maize is limited and breeding for cold tolerance remains a challenge. In this study, we identified QTL associated with cold tolerance during germination and seedling stages. Our results showed a large genetic diversity in cold tolerance within the studied populations. We found several QTL related to plant weight, fluorescence, and chlorophyll content under both cold and control conditions. However, the number and location of QTL differed between these conditions, suggesting a significant genetic discrepancy between optimal and low temperatures. Further studies are needed to identify favorable alleles with significant effects on cold tolerance in maize using different experimental designs.
Article
Agronomy
Mohammed Abdelaziz Sayed, Saad Mohamed Nassar, Ehab Soudi Moustafa, Mohamed Tharwat Said, Andreas Boerner, Alhosein Hamada
Summary: This study identified important genomic regions associated with grain weight and salinity tolerance in barley, as well as pinpointed specific markers linked to vernalization, dwarfing, and dehydrin genes. The evaluation of different barley genotypes revealed that wild and elite barley varieties possess valuable alleles that can enhance salinity tolerance and improve yield under salt stress conditions.
Article
Plant Sciences
Long Li, Zhi Peng, Xinguo Mao, Jingyi Wang, Chaonan Li, Xiaoping Chang, Ruilian Jing
Summary: Developing salt-tolerant crop varieties is crucial to combat soil salinization globally. This study identified novel genes and genetic loci associated with salt responsiveness in wheat, while also highlighting the decline in salt tolerance among Chinese wheat varieties. The findings suggest the importance of considering genetic diversity and selection pressures in breeding for salt tolerance.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
T. S. Aruna, Arpita Srivastava, Bhoopal Singh Tomar, Tusar Kanti Behera, Hari Krishna, Pradeep Kumar Jain, Renu Pandey, Bhupinder Singh, Ruchi Gupta, Manisha Mangal
Summary: This study investigates the genetic mechanisms underlying heat tolerance in hot peppers through the crossbreeding of two genotypes with contrasting heat tolerance characteristics. Phenotypic analysis of the F-2 population and parental lines under high-temperature conditions revealed the presence of 64 significant QTLs affecting heat tolerance. The identified QTLs and SSR markers provide valuable insights for the development of heat-tolerant varieties.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Q. Yi, L. alvarez-Iglesias, R. A. Malvar, M. C. Romay, Pedro Revilla
Summary: A large association panel of 836 maize inbreds revealed a broader genetic diversity of cold tolerance, with predominantly favorable QTL having small effects that are specific to different environments, highlighting the potential of genomic selection in maize breeding for cold tolerance.
THEORETICAL AND APPLIED GENETICS
(2021)
Article
Biotechnology & Applied Microbiology
Fei Zhang, Deborah Velez-Irizarry, Catherine W. Ernst, Wen Huang
Summary: In this study, genome-wide analysis of alternative splicing in skeletal muscle of a Duroc x Pietrain F2 pig population was performed. It was found that the heritability of alternative splicing scores was lower than that of overall gene expression. Additionally, there was little correlation between alternative splicing and overall gene expression. Integration of splice QTL mapping with phenotype QTL mapping identified potential mediators of phenotype QTL effects through alternative splicing.
Article
Fisheries
Lina Wu, Yang Yang, Xi Wang, Zhuoying Weng, Sijie Hua, Duo Li, Junhong Xia, Xiaochun Liu, Zining Meng
Summary: In this study, a high-density genetic linkage map was constructed for the giant grouper, and QTL mapping was performed for growth traits. Six growth-associated QTLs were identified, and candidate genes were validated. Additionally, RNA-seq analysis revealed differentially expressed genes that may play important roles in cell growth, signal transduction, metabolic pathways, and skeletal development.
Article
Agronomy
B. Techale, H. Dong, G. Mihrete, G. Aregash, A. H. Paterson, B. Kassahun
Summary: This study successfully mapped the QTLs for agronomic and yield-related traits in elite Ethiopian sorghum germplasm using three connected mapping populations of different genetic backgrounds. A total of 105 QTLs were identified, and 25 meta QTLs were found on a combined consensus map, providing important references for genetic studies and marker-assisted breeding programs in sorghum. The research also revealed substantial standing variation among the lines, especially in the trait of reduced leaf senescence, which could be utilized in improving drought tolerance of sorghum.
Article
Biotechnology & Applied Microbiology
Ying Zhang, Jie Li, Peng Chu, Ruhua Shang, Shaowu Yin, Tao Wang
Summary: A high-density genetic linkage map was constructed for T. fasciatus, and QTLs and candidate genes associated with growth and cold tolerance traits were identified. These findings are of great significance for the selection and breeding of cold-tolerant or fast-growing T. fasciatus.
Article
Plant Sciences
Vinay Sharma, Sunil S. Gangurde, Spurthi N. Nayak, Anjan S. Gowda, B. S. Sukanth, Supriya S. S. Mahadevaiah, Surendra S. Manohar, Rakeshkumar S. Choudhary, T. Anitha, Sachin S. Malavalli, S. N. Srikanth, Prasad Bajaj, Shailendra Sharma, Rajeev K. Varshney, Putta Latha, Pasupuleti Janila, Ramesh S. Bhat, Manish K. Pandey
Summary: Rising temperatures in semi-arid environments hinder groundnut productivity and quality. Understanding the molecular mechanisms of heat stress tolerance will help address yield losses. QTL analysis identified three QTL clusters that harbor most of the major QTLs for heat tolerant traits, and several candidate genes with potential roles in seed development, plant architecture regulation, yield, plant growth and flowering time regulation, and photosynthesis were also identified. These findings provide a platform for further mapping, gene discovery, and marker development for genomics-assisted breeding of heat-tolerant groundnut varieties.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Ya Min, Evangeline S. Ballerini, Molly B. Edwards, Scott A. Hodges, Elena M. Kramer
Summary: Floral meristems have stem cells that ultimately terminate at a specific time point, leading to variations in floral morphological diversity. Investigating the variation in stamen whorl numbers in Aquilegia species provides insights into the genetic basis of floral meristem termination.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Biotechnology & Applied Microbiology
Xiaolei Zhang, Ming Lu, Aiai Xia, Tao Xu, Zhenhai Cui, Ruiying Zhang, Wenguo Liu, Yan He
Summary: This study identified QTL associated with husk traits in maize and teosinte, revealing genetic relationships between husk length, width, and layers, which provide clues for exploring candidate genes regulating husk growth.
Article
Plant Sciences
Jia Wang, Lin Mao, Zhaoqiong Zeng, Xiaobo Yu, Jianqiu Lian, Jun Feng, Wenying Yang, Jiangang An, Haiying Wu, Mingrong Zhang, Liezhao Liu
Summary: This study utilized QTL mapping, QTL-seq, and RNA sequencing to identify genes controlling protein content in soybean using the Nanxiadou 25 variety. Major QTLs on chromosome 20 were repeatedly detected across multiple environments, indicating potential candidate genes. Through gene expression analysis and clone sequencing, different SNPs and indels were identified as potentially responsible for changes in protein content.
Review
Biochemistry & Molecular Biology
Huaqing Liu, Bohong Zeng, Jialiang Zhao, Song Yan, Jianlin Wan, Zhibin Cao
Summary: Heat stress caused by high-temperature weather poses a serious threat to international food security. Rice, as an important food crop, is frequently affected by heat stress, impacting yield and quality. This article summarizes recent research on quantitative trait loci (QTL) and cloned genes related to heat tolerance in rice. It discusses various response mechanisms, including plasma membrane response, protein homeostasis, reactive oxygen species accumulation, and photosynthesis, as well as regulatory mechanisms. The findings provide new ideas and insights for improving heat tolerance in rice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Jialin Sun, Shuangnan Li, Huijuan Guo, Zhenan Hou
Summary: The study revealed that under different salt stress conditions, the salt-tolerant cotton variety showed better tolerance to saline-alkaline stress and had an internal mechanism to maintain ionic homeostasis.
Article
Agriculture, Dairy & Animal Science
Shuai Guo, Meixuan Chen, Ting Wu, Kailong Liu, Heping Zhang, Jicheng Wang
Summary: The addition of the probiotic Probio-M8 improves the sensory, physicochemical, and functional properties of fermented goat milk, including the formation of organic acids and functional long-chain unsaturated fatty acids, and the reduction of goaty flavor.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Ting Wu, Milad Alizadeh, Bailan Lu, Jinkui Cheng, Ryan Hoy, Miaoyu Bu, Emma Laqua, Dongxue Tang, Junna He, Dongeun Go, Zhizhong Gong, Liang Song
Summary: This study reports on the role of Arabidopsis thaliana SDR4L in transcriptional regulation, which represses embryonic traits post-imbibition by forming a feedback inhibition loop with LEC1 and ABI3. The expression of AtSDR4L is regulated by LEC1 and ABI3, and it has an impact on seedling establishment under suboptimal conditions.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Plant Sciences
Jing Yang, Qingbo Zheng, Yi Wang, Ting Wu, Wei Li, Changpeng Qiu, Xuefeng Xu, Xi Zhang, Zhenhai Han, Xinzhong Zhang
Summary: Glutathione dependent protein post-translational modifications play important roles in plant development and stress response. The protein modifications differed considerably between the juvenile and the adult phase in apple, mainly localized in chloroplast and mitochondrion.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Biotechnology & Applied Microbiology
Yifan Xing, Wenjing Sun, Yuying Sun, Jialin Li, Jie Zhang, Ting Wu, Tingting Song, Yuncong Yao, Ji Tian
Summary: This study investigated the dynamics of apple fruit phosphoproteome in response to light using high-performance liquid chromatography-tandem mass spectrometry analysis. The findings revealed that mitogen-activated protein kinase MdMPK6 directly interacted with and activated transcription factor MdHY5 through phosphorylation under light conditions, thereby promoting anthocyanin accumulation in apple fruit.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Xianglong Yang, Bei Wu, Jing Liu, Zhongyan Zhang, Xuan Wang, Haie Zhang, Xuejun Ren, Xi Zhang, Yi Wang, Ting Wu, Xuefeng Xu, Zhenhai Han, Xinzhong Zhang
Summary: The genetic recombination and interactions among five and two functional variations at MdbHLH25 and MdWDR5A cause complicated phenotype segregation in apple FFR and FCR. Five and two SNPs were identified on the candidate genes MdbHLH25 and MdWDR5A, which affect ethylene production and maintain higher flesh crispness. Understanding these genetic variations contributes to better understanding the effects of QTL and provides potential for marker development in molecular breeding.
PLANT CELL REPORTS
(2022)
Article
Plant Sciences
Qiran Sun, Longmei Zhai, Danrui Zhao, Min Gao, Yue Wu, Ting Wu, Xinzhong Zhang, Xuefeng Xu, Zhenhai Han, Yi Wang
Summary: The kinase MxMPK4-1 and IQ-motif containing protein MxIQM3 form a complex that enhances PM H+-ATPase activity to improve plant's response to iron deficiency stress.
Article
Plant Sciences
Xiaona Wang, Xiaofen Chai, Beibei Gao, Cecilia Deng, Catrin S. Gunther, Ting Wu, Xinzhong Zhang, Xuefeng Xu, Zhenhai Han, Yi Wang
Summary: Nitrogen is essential for plant growth, but increased nitrogen fertilizer application can decrease nitrogen use efficiency and waste resources. In this study, we investigated the mechanism of nitrogen uptake in apple rootstocks under low-nitrogen stress using multi-omics approaches. We found that the transcription factor bHLH130 regulates the flavonoid pathway, thereby influencing nitrogen uptake efficiency.
Article
Plant Sciences
Qinyuan Zhu, Jiahong Lv, Yue Wu, Mengqun Peng, Xinyi Wu, Jie Li, Ting Wu, Xinzhong Zhang, Xuefeng Xu, Yi Wang, Yi Feng
Summary: The MdbZIP74 gene in apple plants negatively regulates osmotic tolerance and its silencing enhances adaptability to moderate drought conditions. It maintains redox balance by regulating cytokinin levels.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Cong Liu, Jian-Zhong Lin, Yan Wang, Ye Tian, He-Ping Zheng, Zheng-Kun Zhou, Yan-Biao Zhou, Xiao-Dan Tang, Xin-Hui Zhao, Ting Wu, Shi-Long Xu, Dong-Ying Tang, Ze-Cheng Zuo, Hang He, Lian-Yang Bai, Yuan-Zhu Yang, Xuan-Ming Liu
Summary: A protein phosphatase, PC1, dephosphorylates Catalase C to negatively regulate H2O2 homeostasis in rice. PC1 inhibits the tetramerization and activity of Catalase C, leading to hypersensitivity to salt and oxidative stresses. Knockout of PC1 improves salt tolerance and rice grain yield under salt stress conditions.
Article
Plant Sciences
Qiran Sun, Danrui Zhao, Min Gao, Yue Wu, Longmei Zhai, Shan Sun, Ting Wu, Xinzhong Zhang, Xuefeng Xu, Zhenhai Han, Yi Wang
Summary: Iron (Fe) deficiency has a significant impact on the growth, development, fruit yield, and quality of apples. In this study, the researchers found that the plasma membrane H+-ATPase MxHA2 plays a role in promoting H+ secretion and root acidification in apple rootstocks under Fe deficiency stress. The kinase MxMPK6-2 is also involved in Fe absorption and interacts with MxHA2. The researchers discovered that MxMPK6-2 positively regulates PM H+-ATPase activity in apple roots, enhancing root acidification under Fe deficiency stress. Phosphorylation of MxHA2 by MxMPK6-2 and the phosphorylation of the Fe deficiency-induced transcription factor MxbHLH104 play a role in enhancing MxHA2 upregulation and PM H+-ATPase activity.
Article
Biochemistry & Molecular Biology
Jiahong Lv, Yi Feng, Lizhong Jiang, Guibin Zhang, Ting Wu, Xinzhong Zhang, Xuefeng Xu, Yi Wang, Zhenhai Han
Summary: In this study, a genome-wide comprehensive analysis of WOX genes in nine Rosaceae species was conducted, and their potential roles in Malus were investigated. The results revealed significant collinearity in Prunus. domestica among the Rosaceae species. The study also categorized the cis-elements in the WOX gene family into three categories and found that overexpression of MdWOX13-1 increased callus weight and enhanced ROS scavenging against drought stress by directly binding to the MdMnSOD promoter.
Article
Plant Sciences
Keting Li, Longmei Zhai, Lizhong Jiang, Qiran Sun, Ting Wu, Xinzhong Zhang, Xuefeng Xu, Zhenhai Han, Yi Wang
Summary: This study found that Fe deficiency induces the expression of MxROP1 in apple at both transcriptional and translational levels. Overexpression of MxROP1, MxROP1DN, and MxROP1CA in apple roots increased ferric chelate reductase activity and the ability to acidify the rhizosphere. MxROP1 negatively regulated the stability of MxZR3.1 protein and affected its interaction with MxbHLH39.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Keting Li, Longmei Zhai, Sitong Fu, Ting Wu, Xinzhong Zhang, Xuefeng Xu, Zhenhai Han, Yi Wang
Summary: In this study, six MdZR genes were identified in apple. The MdZRs were classified into three categories, and MdZR2.2 was found to be significantly upregulated under salt and drought stress. Overexpression of MdZR2.2 improved apple's tolerance to drought and salt stress and their ability to scavenge reactive oxygen species (ROS). Silencing MdZR2.2 in transgenic apple roots resulted in reduced growth and decreased ability to scavenge ROS under salt and drought stress.
Article
Plant Sciences
Yimei Huang, Xiaofen Chai, Xiaona Wang, Beibei Gao, Hui Li, Zhenhai Han, Xuefeng Xu, Xinzhong Zhang, Ting Wu, Yi Wang
Summary: Microorganisms play a crucial role in the growth, health, and ecological function of apples. This study used the Illumina Miseq platform to analyze the bacterial communities in different ecological niches of two scion/rootstock combinations of apples. The results showed significant effects of different niches and rootstock genotypes on the diversity and composition of the bacterial community. Soil properties and plant characters were found to influence the structure of the bacterial community. This study provides valuable insights into the microbial-plant interaction of apple rootstocks.
HORTICULTURAL PLANT JOURNAL
(2023)
Article
Plant Sciences
Yanan Hu, Hualin Sun, Zhenyun Han, Shuai Wang, Ting Wang, Qiqi Li, Ji Tian, Yi Wang, Xinzhong Zhang, Xuefeng Xu, Zhenhai Han, Ting Wu
Summary: The regulation of apple fruit texture during ripening is complex and involves the interaction between ethylene and jasmonate signaling pathways. This study reveals that ERF4 acts as a key protein in the jasmonate pathway, repressing the expression of ethylene biosynthesis genes. This molecular mechanism provides insight into the regulation of fruit ripening in apples.
HORTICULTURAL PLANT JOURNAL
(2022)