Article
Biochemical Research Methods
Bisan Samara, Muhammedin Deliorman, Pavithra Sukumar, Mohammad A. Qasaimeh
Summary: A method of creating cryopreservable high throughput arrays of 3D tumor models using a paper platform is presented, utilizing virtual microwells to facilitate cell aggregation and prevent mixing, allowing for extended storage and drug treatment studies.
Article
Environmental Sciences
P. Rajasulochana, Yaswanth Ganesan, P. Senthil Kumar, S. Mahalaxmi, Fahira Tasneem, Muthamilselvi Ponnuchamy, Ashish Kapoor
Summary: To ensure safe drinking water, a paper-based microfluidic sensor was developed for quantitative estimation of nitrite in water. The sensor, fabricated using a simple punching machine and assisted by smartphone image analysis, showed linear detection of nitrite in the range of 0.1 to 10 ppm.
ENVIRONMENTAL RESEARCH
(2022)
Article
Chemistry, Analytical
Lorena Placer, Isela Lavilla, Francisco Pena-Pereira, Carlos Bendicho
Summary: This study presents the development of a 3D origami microfluidic paper-based analytical device (3D mu PAD) for the determination of iodide and iodate in edible seaweeds by colorimetric detection using a smartphone. The addition of a methacrylate holder improved the interlayer contact in 3D mu PADs, resulting in enhanced sensitivity and precision, as well as real-time monitoring. The assay relies on the formation of a blue triiodide-starch complex at the detection areas of the 3D mu PAD, providing an affordable, fast, and environmentally friendly method for simultaneous determination of inorganic iodine species. Under optimal conditions, the method achieved low limits of detection and quantification for iodide and iodate.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Natalie Uhlikova, M. Ines G. S. Almeida, Ian McKelvie, Richard Morrison, Spas D. Kolev
Summary: This study evaluated the influence of scanner settings, data analysis methods, and physical characteristics of the micro-fluidic paper-based analytical devices (mu PADs) on colorimetric detection. The most sensitive calibration curve was achieved by selecting optimal scanning conditions and a suitable colorimetric system. A flatbed scanner showed greater variation in response under different scanning conditions, while a portable scanner provided consistent responses. Parameters such as gamma correction, scanning resolution, and pixel analysis did not markedly affect the calibration curve. Factors that influenced colorimetric detection included scanner settings, blank color, image acquisition mode, and mu PAD covering. Based on these findings, generic guidelines for optimal data acquisition and mu PAD manipulation have been formulated with respect to mu PAD calibration.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Xinyu Tong, Xianfeng Lin, Nuo Duan, Ziyu Lv, Zhouping Wang, Shijia Wu
Summary: A portable biosensing platform was developed for detecting SDM residues in aquatic food. It showed sensitive detection in the range of 10-1000 ng/mL with low cost, low reagent consumption, and no equipment or operator limitations.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Kaewta Danchana, Hiroshi Iwasaki, Kenta Ochiai, Haruka Namba, Takashi Kaneta
Summary: A paper-based device (PAD) capable of colorimetric detection was developed to determine the presence of glutamate in various food samples. The PAD utilizes an enzymatic and oxidation reaction to detect glutamate, and it is simple, easy to fabricate, and portable. It can successfully determine glutamate in food samples without the need for complicated sample pretreatment.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Tyler S. Larson, Gary L. Glish, Matthew R. Lockett
Summary: Paper-based cultures provide an emerging platform for preparing 3D tissue and tumor-like structures with defined cellular compositions and microenvironments. Spatially resolved quantification of cell viability and drug metabolism was achieved in a stacked structure using liquid chromatography-mass spectrometry. The study showed that cells at the bottom of the stack were more drug-resistant, similar to nutrient-poor center cells of a tumor being more resistant than rapidly dividing cells at the periphery.
ANALYTICA CHIMICA ACTA
(2021)
Article
Food Science & Technology
Xuemei Tang, Ruiqi Su, Haixi Luo, Ying Zhao, Lu Feng, Jian Chen
Summary: The study introduced a method for rapid detection of Aflatoxin B-1 (AFB(1)) using microfluidic paper-based analytical devices (mu PADs), which exhibited superior detection performance in actual food samples and met the testing requirements of countries like China.
Article
Chemistry, Analytical
Inmaculada Ortiz-Gomez, Almudena Rivadeneyra, Jose F. Salmeron, Ignacio de Orbe-Paya, Diego P. Morales, Luis Fermin Capitan-Vallvey, Alfonso Salinas-Castillo
Summary: In this study, a microfluidic paper-based analytical device (mu PAD) with an NFC tag was proposed as a portable, simple and fast colorimetric method for glutathione (GSH) determination. The method took advantage of the oxidizing property of Ag+ on 3,3';,5,5'-tetramethylbenzidine (TMB), which resulted in a blue color fading when GSH was present. By integrating electronic interfaces into the hardware of digital image capture, a smartphone was utilized for the colorimetric determination of GSH. Importantly, this new method exhibited a low detection limit of 1.0 mu M, offering high sensitivity and a simple, fast, portable and low-cost GSH determination within 20 minutes using a colorimetric signal.
Article
Green & Sustainable Science & Technology
Tiancheng Ouyang, Jie Lu, Xiaoyi Hu, Wenjun Liu, Jingxian Chen
Summary: This study investigates the paper-based microfluidic fuel cell through numerical simulation. It reveals that the cell performance decreases with the increase of folding angle and increasing absorbent pad volume is the most direct way to improve performance. Additionally, using materials with better water absorption capacity and increasing the operating temperature appropriately can increase the cell's power density.
Review
Chemistry, Medicinal
Wisarut Khamcharoen, Kantima Kaewjua, Phanumas Yomthiangthae, Ananyaporn Anekrattanasap, Orawon Chailapakul, Weena Siangproh
Summary: In the last decade, the demand for drugs for biomedical applications has increased significantly due to the global increase in diseases. This has led to a need for analytical tools to monitor, treat, investigate, and control drug compounds in various matrices. Microfluidic paper-based analytical devices (μPADs) have gained attention in many analytical applications due to the benefits of their cellulose structure. They offer advantages such as self-pumping capability, low material costs, easy fabrication, and no requirement for external power sources. This review provides an overview of the designs and applications of μPADs in pharmaceutical analysis over the past decade. It discusses device designs, choice of paper material, and fabrication techniques, along with strategies for improving analytical performance and the detection of drug compounds. Recent advances and the challenges and potential of transitioning μPADs from the laboratory to commercialization are also discussed.
CURRENT TOPICS IN MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Ana Carolina Rafanhin Sousa, Cleyton Nascimento Makara, Lais Canniatti Brazaca, Emanuel Carrilho
Summary: In order to detect sulfonamides in milk, a simple and portable microfluidic paper-based analytical device (mu PAD) was developed for precise analysis without the need for pretreatment. This device has good linearity and selectivity, potentially allowing for on-site monitoring of milk quality to reduce adverse conditions associated with high concentrations of sulfonamides.
Article
Chemistry, Analytical
Jinju Xu, Chaoying Tong, Yuanxin Cao, Ziyi Qin, Chunhui Liao, Yuxia Chen, Shuyun Shi, Ying Guo
Summary: In this study, a dual-channel foldable microfluidic paper-based parallel enzymatic reaction system was fabricated for simultaneous detection of AChE and alpha-Glu, as well as screening inhibitors. The developed platform provides a convenient, efficient, sensitive, and specific method for point-of-care testing of disease and development of drugs.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Manassawee Janrod, Monpichar Srisa-Art
Summary: A microfluidic paper-based analytical device (mu PAD) with colorimetric detection was developed for simultaneous determination of urinary oxalate, citrate, and uric acid. The detection methods utilized enzymatic reactions and an indicator displacement assay. The mu PAD showed promising results with linear ranges and low detection limits for the three biomarkers, as well as acceptable accuracy and precision in urine sample testing. This development holds great potential for on-site analysis, providing a simple, fast, affordable, and portable tool for simultaneous determination of the biomarkers.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Ayushi Chauhan, Bhushan J. Toley
Summary: The barrier-free mu PAD device has been developed to detect multiple analytes without the need for wax patterning, improving the limit of colorimetric detection and streamlining large-scale manufacturing.
ANALYTICAL CHEMISTRY
(2021)