Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 117, Issue 9, Pages 2827-2841Publisher
WILEY
DOI: 10.1002/bit.27458
Keywords
alveolar air-tissue interface; alveolus mimic; gelatin nanofibers; lung-on-a-chip; physiological strain
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Funding
- Agence Nationale de la Recherche (ANR) [ANR-17-CE09-0017]
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We report on the development of a new model of alveolar air-tissue interface on a chip. The model consists of an array of suspended hexagonal monolayers of gelatin nanofibers supported by microframes and a microfluidic device for the patch integration. The suspended monolayers are deformed to a central displacement of 40-80 mu m at the air-liquid interface by application of air pressure in the range of 200-1,000 Pa. With respect to the diameter of the monolayers, that is, 500 mu m, this displacement corresponds to a linear strain of 2-10% in agreement with the physiological strain range in the lung alveoli. The culture of A549 cells on the monolayers for an incubation time of 1-3 days showed viability in the model. We exerted a periodic strain of 5% at a frequency of 0.2 Hz for 1 hr to the cells. We found that the cells were strongly coupled to the nanofibers, but the strain reduced the coupling and induced remodeling of the actin cytoskeleton, which led to a better tissue formation. Our model can serve as a versatile tool in lung investigations such as in inhalation toxicology and therapy.
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