Article
Reproductive Biology
M. S. Ansari, B. A. Rakha, S. Akhter, A. Akhter, E. Blesbois, J. Santiago-Moreno
Summary: The study showed that using 0.5 mM GSH in the extender during cryopreservation of Indian red jungle fowl sperm can improve sperm quality and fertility by enhancing antioxidant potential and alleviating oxidative stress.
Article
Genetics & Heredity
M. Kanakachari, R. N. Chatterjee, M. R. Reddy, M. Dange, T. K. Bhattacharya
Summary: NGS sequencing was used to investigate the mitochondrial genomes of Indian native chickens and Red Jungle fowl, revealing 23 haplotypes and six significant clades. The study confirmed the Indian Red Jungle fowl as the origin of all Indian breeds.
FRONTIERS IN GENETICS
(2023)
Article
Agriculture, Dairy & Animal Science
Bushra Allah Rakha, Muhammad Sajjad Ansari, Sumiyyah Zuha, Shamim Akhter
Summary: The Indian red jungle fowl population is declining in its natural habitat. Assisted reproductive technologies, such as sperm cryopreservation, can help conserve this species. This study investigates the effect of ascorbic acid on the freeze-thawing ability of Indian red jungle fowl sperm.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Agriculture, Dairy & Animal Science
B. A. Rakha, M. S. Ansari, S. Akhter, A. Akhter, E. Blesbois
Summary: The study showed that intravaginal insemination at a depth of 4 cm enhances fertility outcomes of frozen-thawed Indian red jungle fowl semen compared to 2 cm depth.
ANIMAL BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Caroline Lindholm, Petros Batakis, Jordi Altimiras, John Lees
Summary: Research finds that intermittent fasting has significant effects on gene expression in the liver, particularly genes related to energy metabolism. Intermittent fasting promotes increased proliferative potential in both birds and mammals, indicating that the health benefits of periodic caloric restriction are not limited to mammals alone.
Article
Biochemistry & Molecular Biology
Giuliana Cardozo, Mauricio Mastrogiovanni, Ari Zeida, Nicolas Viera, Rafael Radi, Anibal M. Reyes, Madia Trujillo
Summary: Human peroxiredoxin 3 (HsPrx3) is oxidized and hyperoxidized by (f)FA-OOHs, including arachidonic acid and eicosapentaenoic acid peroxidation products. Biophysical methods and molecular dynamic simulations suggest that HsPrx3 can bind hydrophobic structures and interact with the hydroperoxide group of (f)FA-OOHs. Kinetic simulation analysis supports that mitochondrial (f)FA-OOHs can lead to HsPrx3 hyperoxidation.
Article
Agriculture, Multidisciplinary
B. A. Rakha, M. S. Ansari, S. Akhter, A. Akhter, E. Blesbois, J. S. Moreno
Summary: The experiment evaluated the metabolic activity of Indian red jungle fowl sperm during cryopreservation using MTT reduction assay. The MTT test was compared with sperm motility and viability data. Significant positive associations were found between MTT reduction rate and motility (r=0.85) and viability (r=0.84). The cryopreservation process led to declines in motility, viability, and metabolic status, with the highest decrease observed after freeze-thawing. The MTT reduction assay is a simple and reliable method to assess mitochondrial redox activity in sperm of this threatened species.
JOURNAL OF ANIMAL AND PLANT SCIENCES-JAPS
(2023)
Article
Biochemistry & Molecular Biology
Eduardo Esteban-Zubero, Laura Lopez-Pingarron, Jose Manuel Ramirez, Marcos Cesar Reyes-Gonzales, Francisco Javier Azua-Romeo, Marisol Soria-Aznar, Ahmad Agil, Jose Joaquin Garcia
Summary: This study evaluated the in vivo effects of melatonin treatment on oxidative damage in the liver in an experimental model of ischemia-reperfusion. Melatonin pretreatment reduced liver ischemia-reperfusion injury and oxidative stress response, making it a potential candidate for co-treatment in patients with hepatic vascular occlusion followed by reperfusion.
Article
Biochemistry & Molecular Biology
Azza A. A. Attia, Jehan M. M. Sorour, Neama A. A. Mohamed, Tagreed T. T. Mansour, Rasha A. A. Al-Eisa, Nahla S. S. El-Shenawy
Summary: This study aimed to investigate the protective role of vitamin E in improving cardiotoxicity induced by cyclophosphamide in rats. The results showed that vitamin E could attenuate the elevation of cardiac markers, increase in lipid profiles, reduction in antioxidant enzyme activities, and increase in lipid peroxidation. In addition, vitamin E was found to improve the histopathological alterations caused by cyclophosphamide.
Article
Biochemistry & Molecular Biology
Yang Liu, Yuan Wang, Zhi Lin, Rui Kang, Daolin Tang, Jiao Liu
Summary: SLC25A22 acts as a metabolic repressor of ferroptosis by producing glutathione and monounsaturated fatty acids, providing a previously unrecognized defense pathway against ferroptotic cell death.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Food Science & Technology
Phung Diep Huy Vu, Akkaratch Rodklongtan, Pakamon Chitprasert
Summary: The study found that the encapsulant weight ratio of whey protein isolate-lignin complex significantly affects the survival rate of spray-dried probiotics, with the 9:1 ratio offering the best protection. Lignin improves the survival of probiotics by enhancing the structure and antioxidant properties of WPI.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Kathe Elise Kittelsen, Pall Gretarsson, Per Jensen, Jens Peter Christensen, Ingrid Toftaker, Randi Oppermann Moe, Guro Vasdal
Summary: Fractures and deviations to the keel bone are common in commercial laying hens, with modern genetics and selection for efficient egg production being important factors. There are significant differences in prevalence between Red jungle fowl and White Leghorn hens, with the latter showing a higher rate of fractures.
Article
Cell Biology
Hiromi Kurokawa, Atsushi Taninaka, Hidemi Shigekawa, Hirofumi Matsui
Summary: Dabigatran is a novel oral anticoagulant that inhibits thrombins, but its use may increase the risk of gastrointestinal bleeding. Research has shown that its main component, dabigatran etexilate, exhibits cytotoxicity that can be inhibited by ascorbic acid. These cytotoxic effects are likely mediated via mitROS production.
Article
Agriculture, Dairy & Animal Science
Alireza Behnamifar, Shaban Rahimi, Mohammad Amir Karimi Torshizi, Mohsen Sharafi, J. L. Grimes
Summary: This study investigated the effects of alpha-lipoic acid supplementation on the semen quality and fertility parameters of aging breeder roosters, finding that appropriate levels of ALA can improve sperm quality, while higher doses do not result in further improvement.
Review
Biochemistry & Molecular Biology
Onchuma Mueangson, Aongart Mahittikorn, Nsoh Godwin Anabire, Wanida Mala, Manas Kotepui
Summary: This study examined the relationship between malondialdehyde (MDA) concentrations and Plasmodium infections. The results showed that MDA concentrations were significantly elevated in patients with malaria compared to uninfected controls, indicating an increase in oxidative stress and lipid peroxidation. MDA levels can be a valuable biomarker for evaluating these processes in individuals with malaria.
Review
Agriculture, Dairy & Animal Science
J. Santiago-Moreno, E. Blesbois
Summary: Over the last century, various reproductive biotechnologies have been developed to improve poultry breeding stocks and conserve genetic diversity. These technologies include artificial insemination, semen storage, PGC methodologies, and gonad tissue storage and transplantation. Combining these technologies allows for the conservation and rapid reconstitution of poultry populations.
Article
Biochemistry & Molecular Biology
Julian Santiago-Moreno, Belen Pequeno, Belen Martinez-Madrid, Cristina Castano, Paula Boveda, Rosario Velazquez, Adolfo Toledano-Diaz, Manuel Alvarez-Rodriguez, Heriberto Rodriguez-Martinez
Summary: This study identified the presence of AQPs in frozen-thawed sperm of wild ruminants and investigated the influence of the interaction between photoperiod and thyroxine on AQP expression and testosterone secretion. The results showed that thyroxine and melatonin treatments can significantly alter sperm viability and the distribution of AQPs. Furthermore, the study suggested that the impact of photoperiod/melatonin on AQP expression might be indirectly exerted through changes in testosterone concentration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Veterinary Sciences
Emma O'Brien, Cristina Castano, Adolfo Toledano-Diaz, Jose Nestor Caamano, Carlos Hidalgo, Luis Eusebio Fidalgo, Ana Maria Lopez-Beceiro, Milagros Cristina Esteso, Ramon Balsera, Pedro Garcia-Casado, Ewa Lukaszewicz, Julian Santiago-Moreno
Summary: By using domestic chicken as a model, the study found that LR84 extender and 4% DMA cryoprotectant concentration are the best options for freezing the semen of Cantabrian capercaillie.
VETERINARY MEDICINE AND SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
D. A. Galarza, D. Jara, E. B. Paredes, J. X. Samaniego, M. S. Mendez, M. E. Soria, F. Perea, E. Munoz-Leon, J. Santiago-Moreno
Summary: This study assessed the suitability of BoviPure-DGC and swim-up methods for selecting dog epididymal sperm and their effects on sperm quality. The results showed that BoviPure-DGC had a higher sperm retrieval rate and improved sperm motility and membrane integrity compared to the swim-up method, both in individual and pooled samples.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Cell Biology
Paula Boveda Gomez, Rosario Velazquez, Lucia Martinez-Fresneda, Octavio Mejia, Marta Oteo, Adolfo Toledano-Diaz, Cristina Castano, Milagros Cristina Esteso, Rodolfo Ungerfeld, Antonio Lopez-Sebastian, Julian Santiago-Moreno
Summary: This study examined the influence of induced changes in prolactin secretion on sperm cryoresistance in ibex and mouflon. The findings suggest that high levels of blood plasma prolactin negatively affect the cryoresistance of sperm in these animals.
BIOPRESERVATION AND BIOBANKING
(2023)
Article
Biology
H. Woelders, A. A. C. de Wit, B. Engel, B. Hulsegge, I. Grasseau, E. Blesbois, B. Bernal, J. Santiago-Moreno
Summary: This study modified a classical chicken semen diluent to have lowered osmolalities, allowing for cold storage and freezing of the semen with minimal loss of membrane integrity and motility. Different cryoprotective agents and cooling rates were tested to determine their effects on post-thaw sperm performance. The study found that specific combinations of cryoprotective agent concentration and cooling rate yielded the best results.
Article
Fisheries
Shaista Bibi, Rabea Ejaz, Muhammad Amjad Awan, Javeria Arshad, Bushra Allah Rakha, Muhammad Sajjad Ansari, Iqra Shakir, Shamim Akhter
Summary: This study evaluated the efficacy of different extenders and cryoprotectants for cryopreservation of Labeo rohita milt. The results showed that modified Kurokura-2 extender (KE) with methanol as cryoprotectant proved to be the best diluent, improving sperm motility and fertilization rate.
AQUACULTURE RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Rodolfo Ungerfeld, Julia Giriboni, Adolfo Toledano-Diaz, Madeleine Guerrero, Julian Santiago-Moreno
Summary: Transrectal ultrasonic-guided massage of the accessory sex glands (TUMASG) is a technique used for semen collection. Administering carbetocin before TUMASG can shorten the collection time and decrease electrical stimuli, but it has minimal effects on semen quality.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Reproductive Biology
C. M. Picazo, C. Castano, P. Boveda, A. Toledano-Diaz, R. Velazquez, B. Pequeno, M. C. Esteso, J. Gadea, S. Villaverde-Morcillo, J. Cerdeira, J. Santiago-Moreno
Summary: This study compared the effects of slow freezing and vitrification on different cell types in canine and wild boar testicular tissue. The results showed that vitrification had higher cell viability and DNA integrity in dogs, while either method could be used in wild boars.
Article
Reproductive Biology
Emma O'Brien, Clara Malo, Cristina Castano, Pedro Garcia-Casado, Adolfo Toledano-Diaz, Belen Martinez-Madrid, Heriberto Rodriguez-Martinez, Manuel Alvarez-Rodriguez, Julian Santiago-Moreno
Summary: This study investigated the presence and distribution of aquaglyceroporin 3 (AQP-3) in dromedary camel sperm, as well as its role in cryoinjury. The results showed that AQP-3 expression was not associated with the individual sperm response to freezing-thawing process. Additionally, sperm head morphometry was not a reliable predictor of cryosurvival.
Article
Veterinary Sciences
Julia Giriboni, Eva Martinez-Nevado, Juncal Garcia, Rosario Velazquez, Adolfo Toledano-Diaz, Rodolfo Ungerfeld, Julian Santiago-Moreno
Summary: Developing strategies for population control is crucial in both captive wildlife and production animals. Immunization against GnRH is an effective method for reduction of testosterone and sperm quality, but its effectiveness varies with species and treatment repetition.
Article
Veterinary Sciences
Patricia Peris-Frau, Julia Benito-Blanco, Eva Martinez-Nevado, Adolfo Toledano-Diaz, Cristina Castano, Rosario Velazquez, Belen Pequeno, Belen Martinez-Madrid, Milagros C. Esteso, Julian Santiago-Moreno
Summary: This study investigated the cryopreservation of testicular tissue using slow freezing and vitrification methods in diverse wild species. The results showed that the effectiveness of each method varied depending on the species and cell type.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Belen Pequeno, Cristina Castano, Manuel Alvarez-Rodriguez, Paula Boveda, Maria Gemma Millan de la Blanca, Adolfo Toledano-Diaz, Diego Andres Galarza, Heriberto Rodriguez-Martinez, Belen Martinez-Madrid, Julian Santiago-Moreno
Summary: The study aimed to investigate the influence of osmotic changes on the location of aquaporins (AQPs) in spermatozoa during freeze-thawing process. Individual variability in terms of freezeability was observed among rams and their ejaculates. AQP3 expression in the mid- and principal piece of spermatozoa was found to be higher in frozen-thawed samples compared to fresh specimens in the good freezability group, but such differences were not detected in the poor freezability group. AQP3 expression is associated with sperm cryotolerance and can be used as a biomarker.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Agriculture, Dairy & Animal Science
J. Santiago-Moreno, A. Toledano-Diaz, C. Castano, R. Velazquez, P. Boveda, E. O'Brien, P. Peris-Frau, B. Pequeno, B. Martinez-Madrid, M. C. Esteso
Summary: Reproductive technologies can help protect wild ruminant species from extinction and increase the number of animals. Efforts have focused on preserving sperm, oocytes, and embryos. Cryopreservation of sperm is the most commonly used and cheapest method for germplasm storage. New reproductive biotechnologies, such as ultra-rapid freezing techniques, have been developed. However, the success of artificial insemination using frozen-thawed sperm is not always satisfactory in wild small ruminants. Epididymal sperm shows higher cryoresistance than ejaculated sperm, and the seasonal variation in testosterone and prolactin levels is involved in sperm cryoresistance.
Article
Biology
Adrian Martin, Cristina Castano, Emma O'Brien, Adolfo Toledano-Diaz, Rafael Guerra, Felix Gomez-Guillamon, Julian Santiago-Moreno
Summary: This study examines the effect of equilibration time with extender on the variables of ultra-rapidly frozen-thawed sperm from wild ruminants. The results show that equilibration improves the quality and viability of sperm. Epididymal sperm demonstrates greater cryoresistance, while ejaculated sperm shows no dimensional changes before and after equilibration.
