Journal
BIOLOGY OF REPRODUCTION
Volume 103, Issue 4, Pages 791-801Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/biolre/ioaa114
Keywords
capacitation; energy production; glucose uptake; glycolysis; oxidative phosphorylation
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Funding
- Eunice Kennedy Shriver National Institute of Child Health and Human Development [HD088571, HD-038082]
- Male Contraceptive Initiative
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Mammalian sperm are stored in the epididymis in a dormant state. Upon ejaculation, they must immediately start producing sufficient energy to maintain motility and support capacitation. While this increased energy demand during capacitation is well established, it remains unclear how mouse sperm modify their metabolism to meet this need. We now show that capacitating mouse sperm enhance glucose uptake, identifying glucose uptake as a functional marker of capacitation. Using an extracellular flux analyzer, we show that glycolysis and oxidative phosphorylation increase during capacitation. Furthermore, this increase in oxidative phosphorylation is dependent on glycolysis, providing experimental evidence for a link between glycolysis and oxidative phosphorylation in mouse sperm.
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