Article
Biotechnology & Applied Microbiology
Patrawadee Yaiwong, Siriporn Anuthum, Padchanee Sangthong, Jaroon Jakmunee, Suwussa Bamrungsap, Kontad Ounnunkad
Summary: This study presents the development of a simple, reliable, highly sensitive, and label-free aptasensor based on polyethyleneimine-coated gold nanoparticles (PEI-AuNPs) for the quantification of alpha-fetoprotein (AFP). The aptasensor is fabricated using a disposable ItalSens screen-printed electrode (SPE) and detects AFP concentration through the electrochemical response of toluidine blue (TB) intercalating into the aptamer-modified electrode after binding with the target. The assay exhibits a linear range of detection of 10 to 50000 pg mL(-1) with a limit of detection (LOD) of 9.5 pg mL(-1) in human serum.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Hosna Zare, Zahra Meshkat, Behnaz Hatamluyi, Majid Rezayi, Kiarash Ghazvini, Mohammad Derakhshan, Mojtaba Sankian, Alireza Neshani, Ehsan Aryan
Summary: A new type of label-free electrochemical biosensor was developed for specific targeting of M. simiae, showing excellent selectivity and a wide linear range. The biosensor exhibited high precision and reproducibility, providing a low-cost, label-free, and faster detection analysis method.
Article
Nanoscience & Nanotechnology
Jilai Jia, Xiaoping Du, Xuqin Lv, Fang Xie, Jiaojiao Zhou, Jie Cai
Summary: A DNA-mediated growth strategy was developed in this study for the synthesis of platinum nanoparticles (PtNPs) on the surface of graphene oxide (GO). The resulting GO-DNA-PtNPs exhibited excellent peroxidase-mimicking activity, and the activity could be inhibited by Hg(2+) due to the specific trapping of Hg2+ by thymine bases (T-Hg2+-T) and in situ reduction of Hg2+ by PtNPs on the GO surface. The sensor showed good linear range (from 1 pM to 1 μM) and a detection limit of 2.2 pM for Hg2+ analysis, demonstrating excellent analytical performance in complex lake waters.
ACS APPLIED NANO MATERIALS
(2023)
Article
Chemistry, Analytical
Chen-chen Li, Yue-hong Dong, Xiaoran Zou, Xiliang Luo, Dazhong Shen, Juan Hu, Chun-yang Zhang
Summary: A label-free and template-free chemiluminescent biosensor, termed hmC-GLIB-IAS, has been developed for sensitive detection of 5hmC in genomic DNA. This method shows advantages of bisulfite-free, improved sensitivity, and genome-wide analysis of 5hmC at constant reaction temperature. It can detect 5hmC with a high reproducibility and accuracy without isotope-labeled substrates or specific antibodies.
ANALYTICAL CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Tongtong Chen, Feifei Wu, Yang Li, Hussein E. Rozan, Xiguang Chen, Chao Feng
Summary: A label-free photoluminescence immune-detection platform based on functionalized diatom frustules was developed, showing high sensitivity and a wide detection range. The platform has the potential for sensitive quantitative analysis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Elena Alina Chiticaru, Celina Maria Damian, Luisa Pilan, Mariana Ionita
Summary: This study proposes a simple, label-free, and cost-effective DNA detection platform using screen-printed carbon electrodes modified with reduced graphene oxide and gold nanoparticles. The preparation process and electrochemical properties of the modified electrodes were characterized and confirmed. The introduction of gold nanoparticles onto the electrode surface enhanced surface conductivity and sensitivity in detection.
Article
Nanoscience & Nanotechnology
Aisha Javed, Shah Rukh Abbas, Muhammad Uzair Hashmi, Noor Ul Ain Babar, Irshad Hussain
Summary: The study proposes a label-free electrochemical detection method for Mycobacterium tuberculosis (Mtb) using the insertion sequence (IS6110). A graphene oxide-chitosan nanocomposite (GO-CHI) was used to fabricate a positively functionalized electrochemical sensor for Mtb detection, showing high sensitivity and specificity even in raw sputum samples without DNA purification.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Biophysics
Mahdi Malmir, Jalal Arjomandi, Abolfazl Ghafouri Khosroshahi, Mohammadreza Moradi, Hu Shi
Summary: The study introduced a novel biosensor for detecting Y-chromosome DYS14 DNA sequence in the blood plasma of pregnant women, with high efficiency and sensitivity. The results demonstrated the suitability of the biosensor for fetal sex determination in pregnant women between the 7th and 9th weeks of gestation.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Yulan Tian, Ping Zhu, Yating Chen, Xiaoyu Bai, Liping Du, Wei Chen, Chunsheng Wu, Ping Wang
Summary: In this study, a novel piezoelectric aptasensor was designed for the detection of OA, with aptamers and quartz crystal microbalance used for signal detection, and gold nanoparticles for signal amplification. The biosensor showed a wide linear detection range and low detection limit.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemistry & Molecular Biology
Li Li, Zhengbo Chen
Summary: In this study, a high specificity, flexibility, and simplicity aptasensor strategy was designed using a triblock DNA probe. It was found that the polyA fragment could assemble on the gold electrode surface via polyA instead of traditional Au-S bonds, leading to improved hybridization stability. The electrochemical aptasensor showed good repeatability, stability, and specificity.
BIOELECTROCHEMISTRY
(2023)
Article
Biophysics
Ruiyun Zhou, Chen Wang, Yuxin Huang, Kang Huang, Yingli Wang, Wendao Xu, Lijuan Xie, Yibin Ying
Summary: This study investigates the integration of graphene with THz metasurface into a microfluidic cell for sensitive biosensing in liquid environment, demonstrating high sensitivity in detecting DNA sequences of Escherichia coli O157:H7. The results suggest the potential of the proposed graphene-metasurface microfluidic biosensor for label-free biosensing applications.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Omair Adil, Seth B. Eddington, Keith T. Gagnon, Mohtashim H. Shamsi
Summary: Overgrowth of short tandem repeat sequences in genes can cause neurodegenerative disorders. These repeat sequences are ideal targets for label-free electrochemical detection, but their secondary structures and sporadic repeats can complicate detection. By analyzing different nucleic acid microprobe backbones, the study found that the lock-nucleic acid (LNA) microprobe can specifically detect tandem repeats and perform better with the presence of Mg2+. The LNA-based platform shows potential for the development of a reliable biosensor for genetic neurodegenerative disorders.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Chitra P. Kurup, Noor F. Mohd-Naim, Natasha A. Keasberry, Siti N. A. Zakaria, Vipul Bansal, Minhaz U. Ahmed
Summary: A molybdenum sulfide/zirconium oxide/Nafion (MoS2/ZrO2/ Naf) based electrochemiluminescence (ECL) aptasensor was developed for selective and ultrasensitive detection of ApoA1. The modified nanocomposite layer allowed immobilization of ApoA1 aptamers, resulting in enhanced ECL signal. The aptasensor exhibited outstanding sensitivity and a wide linear dynamic range for ApoA1 detection. The proposed assay showed significant improvement in detection limit compared to the commercially available ELISA method.
Article
Chemistry, Analytical
Brendan Gilbride, Gustavo Marcal Schmidt Garcia Moreira, Michael Hust, Cuong Cao, Linda Stewart
Summary: The study developed an immune detection assay using ferromagnetic gold nanoparticles to detect Mycobacterium tuberculosis and differentiate different strains. The nanoparticles' intrinsic magnetic capability was utilized for sample preparation and target bacterial cell capture, providing a quick confirmation of pathogen identification.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
M. R. Ali, M. S. Bacchu, S. Das, S. Akter, M. M. Rahman, M. Aly Saad Aly, M. Z. H. Khan
Summary: A label-free electrochemical DNA biosensor for the selective detection of the foodborne bacterial pathogen Shigella flexneri has been developed. The biosensor exhibits a wide dynamic range, high specificity, and excellent recovery in detecting S. flexneri in food samples.
Article
Chemistry, Analytical
Mahmoud Amouzadeh Tabrizi, Josep Ferre-Borrull, Lluis F. Marsal
SENSORS AND ACTUATORS B-CHEMICAL
(2020)
Article
Multidisciplinary Sciences
Mahmoud Amouzadeh Tabrizi, Josep Ferre-Borrull, Lluis F. Marsal
SCIENTIFIC REPORTS
(2020)
Article
Chemistry, Analytical
Mahmoud Amouzadeh Tabrizi, Josep Ferre-Borrull, Lluis F. Marsal
Article
Chemistry, Analytical
Mahmoud Amouzadeh Tabrizi, Josep Ferre-Borrull, Lluis F. Marsal
SENSORS AND ACTUATORS B-CHEMICAL
(2020)
Article
Biochemistry & Molecular Biology
Maryam Besharati, Mahmoud Amouzadeh Tabrizi, Fatemeh Molaabasi, Reza Saber, Mojtaba Shamsipur, Javad Hamedi, Saman Hosseinkhani
Summary: Two biosensing methods utilizing NAD(P)H-dependent TetX2 enzyme activity and thionine were developed to detect tetracycline in milk. The methods showed great potential for being portable instruments for on-site TC measurements.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Article
Chemistry, Physical
J. T. Domagalski, E. Xifre-Perez, M. A. Tabrizi, J. Ferre-Borrull, L. F. Marsal
Summary: This work presents a process to provide anodic alumina nanotubes with magnetic responsivity based on magnetic nanoparticles, demonstrating their motion under a magnetic field and the potential for selective functionalization and stimuli-responsive load release. The study shows protease-triggered release of fluorescent molecules loaded inside the magnetic nanotubes through selective functionalization, giving insights into potential applications like drug delivery and biosensing.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Biophysics
Mahmoud Amouzadeh Tabrizi, Juan P. Fernandez-Blazquez, Dahiana Mojena Medina, Pablo Acedo
Summary: A novel molecularly imprinted polymer-based electrochemical sensor has been developed for the determination of SARS-CoV-2-RBD. The fabricated sensor showed high selectivity, sensitivity, and stability in detecting SARS-CoV-2-RBD in saliva samples. The sensor exhibited a good response in a concentration range of 2.0 to 40.0 pg mL(-1) with a low detection limit of 0.7 pg mL(-1).
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Mahmoud Amouzadeh Tabrizi, Leila Nazari, Pablo Acedo
Summary: A photoelectrochemical aptasensor for quantitatively measuring the Sars-Cov-2 receptor-binding domain has been developed, utilizing nanomaterial fabrication, electrode modification, and aptamer immobilization. The sensor showed a detection range of 0.5-32.0 nM and a LOD of 0.12 nM, with an affinity of 3.4 nM. The sensor was successfully applied for spiked Sars-Cov-2 RBD measurement in human saliva samples, with interference studies conducted on other biomaterials.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Mahmoud Amouzadeh Tabrizi, Pablo Acedo
Summary: A novel electrochemical aptasensor was developed for the highly sensitive, selective, and cost-effective determination of CRP, showing superior analytical performance and applicability for CRP detection in human plasma. The proposed RNA aptasensor outperformed previously reported CRP aptasensors.
Article
Chemistry, Analytical
Mahmoud Amouzadeh Tabrizi, Pablo Acedo
Summary: In this study, an electrochemical impedance spectroscopy-based aptasensor was developed for the determination of the SARS-CoV-2 receptor-binding domain. The sensor showed high sensitivity and accuracy in detecting SARS-CoV-2-RBD in human saliva samples, with a limit of detection of 7.0 pM.
Article
Chemistry, Physical
Mahmoud Amouzadeh Tabrizi, Pablo Acedo
Summary: In this study, an electrochemical membrane-based aptasensor was developed for the detection of the SARS-CoV-2 receptor-binding domain. The sensor showed high sensitivity and selectivity, and was able to detect low concentrations of SARS-CoV-2-RBD in human saliva samples.
APPLIED SURFACE SCIENCE
(2022)
Article
Chemistry, Analytical
Mahmoud Amouzadeh Tabrizi, Pablo Acedo
Summary: An interdigitated electrochemical immunosensor for the determination of PCT has been developed. The sensor showed good selectivity and stability, with a detection range of 2.5-800 pg/mL and a limit of detection of 0.36 pg/mL.
Article
Chemistry, Analytical
Mahmoud Amouzadeh Tabrizi
Summary: A highly sensitive electrochemical biosensor was designed to detect the RNA-dependent RNA polymerase (RdRP) of SARS-CoV-2. The electrochemical signal was amplified using surface-initiated reversible addition-fragmentation-chain-transfer polymerization. The PNA-based sensor showed high selectivity to SARS-CoV-2 RdRP and had a lower limit of detection compared to previous sensors.
Article
Chemistry, Analytical
Mahmoud Amouzadeh Tabrizi
Summary: In this study, a simple method was used to fabricate a microneedle electrode (MNE). A commercial self-dissolving microneedle patch was first used to create a mold, which was then filled with conductive platinum paste and cured. The Pt-MNE was characterized using CV, EIS, and SEM, and its electrochemical applicability was demonstrated by immobilizing glutamate oxidase enzyme to detect glutamate.
Article
Biochemistry & Molecular Biology
Priyanga Kumar, Noel Nesakumar, Jayashree Gopal, Sakthivel Sivasubramanian, Srinivasan Vedantham, John Bosco Balaguru Rayappan
Summary: This study developed an electrochemical biosensor for measuring MG levels in the plasma of type-2 diabetes mellitus patients. The biosensor showed a wide linear concentration range, high sensitivity, and a 90% correlation with ELISA data. It also exhibited a significant correlation with HbA1c and fasting plasma glucose levels.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Cristina Tortolini, Valeria Gigli, Antonio Angeloni, Federico Tasca, Nguyen T. K. Thanh, Riccarda Antiochia
Summary: The development of a novel voltammetric immunosensor for the detection of salivary MMP-8 at the point-of-care is described. The sensor showed good performance and comparable results to the conventional ELISA method when tested in real saliva samples. This biosensor is single-use, cost-effective, and requires a small quantity of test medium and a short preparation time.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Rimsha B. Jamal, Ulrich Bay Gosewinkel, Elena E. Ferapontova
Summary: Pathogen-triggered infections are a severe global threat to human health. Researchers have developed a fast and inexpensive electrocatalytic aptamer assay for the specific and ultrasensitive detection of E. coli, allowing for timely treatment and prevention. The method is fast, sensitive, and can be used in field and point-of-care applications for analysis of bacteria in the human environment.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Miao Liu, Yuwei Wang, Shanshan Tang, Wei Wang, Axin Liang, Aiqin Luo
Summary: An unlabeled ratiometric molecular imprinted electrochemiluminescence sensor was developed for the determination of trace uric acid using MXene@NaAsc, CdSe@ZnS quantum dots, and molecularly imprinted polymer composites modified glass carbon electrode. This sensor, with easy preparation, great selectivity, and excellent sensitivity, successfully detected uric acid in human serum.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Charlotte Da Silva, Camille Lamarche, Carole Pichereaux, Emmanuelle Mouton-Barbosa, Gauthier Demol, Sebastien Boisne, Etienne Dague, Odile Burlet-Schiltz, Flavien Pillet, Marie-Pierre Rols
Summary: Low-energy electron beams (LEEB) are a safe and practical sterilization solution for industrial applications. To address the limitations of LEEB, we developed a low-energy pulsed electron beam generator (LEPEB) that can effectively and efficiently eradicate bacteria in a wide range of industrial applications.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Daniel Dobrovodsky, Ales Danhel, Daniel Renciuk, Jean-Louis Mergny, Miroslav Fojta
Summary: In this study, N-methyl mesoporphyrin IX (NMM) was utilized as a voltammetric probe for the electrochemical detection of G4s. The detection of NMM was achieved by cyclic voltammetry on a hanging mercury drop electrode (HMDE) with a limit of detection (LOD) of 40 nM. The reduction signal of NMM was found to be significantly higher when G4 oligodeoxynucleotides (G4 ODNs) were present compared to single- or double-stranded ODNs or unfolded ODNs capable of forming G4s. Gradual transition from unstructured single strand to G4, induced by increasing concentrations of the G4 stabilizing K+ ions, was detected for the first time using electrochemical methods. Circular dichroism spectroscopy provided support for the obtained results. This work expands on the utilization of electrochemical probes for DNA secondary structure recognition and offers a proof of principle for the development of novel electroanalytical methods for nucleic acid structure studies.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Mengjiao Zhu, Yu Liu, Meiyue Wang, Tao Liu, Zhenyu Chu, Wanqin Jin
Summary: Early rapid diagnosis of COVID-19 is crucial for reducing the risk of severe symptoms and loss of lung function. Researchers have proposed an ultrafast and ultrasensitive DNA sensor that can accurately detect the virus in a short period of time, with outstanding selectivity.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Fatima-Zahra Ait-Itto, James A. Behan, Mathieu Martinez, Frederic Barriere
Summary: This study investigated the feasibility of using ancient marine sediments as inoculum for bioanode development in microbial fuel cells (MFC). The results showed the presence of two exoelectrogenic bacterial genera in these iron-rich sediments and confirmed that the development of the bioanode derived from the native microbiota. This study has important implications for understanding the role of these bacteria in broader paleoenvironmental phenomena.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Issa Fall, Bastien Doumeche, Sofiene Abdellaoui, Caroline Remond, Harivony Rakotoarivonina, Marjorie Ochs
Summary: This article presents a novel electrochemical tool based on lignin-coated paper electrodes for the detection and characterization of ligninolytic activity. The suitability of this method has been demonstrated using a catalaseperoxidase isolated from Thermobacillus xylanilyticus.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Chenyi Zhuo, Dehong Yu, Jiuying Cui, Zichun Song, Qianli Tang, Xianjiu Liao, Zhao Liu, Ning Xin, Lu Lou, Fenglei Gao
Summary: A label-free detection strategy for quantification of Apolipoprotein A4 (Apo-A4) has been developed based on rolling circle amplification (RCA) and proximity hybridization-induced DNAzyme-driven bipedal DNA walker. This method shows high sensitivity and selectivity for a wide range of protein targets.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Niloufar Fattahi, Jeffrey Reed, Evan Heronemus, Priyasha Fernando, Ryan Hansen, Prathap Parameswaran
Summary: In this study, polyethylene glycol hydrogels were developed as protective coatings for electroactive biofilms, improving their viability under low resource conditions and ammonia-N shocks.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Xiaopeng Hou, Qiongyi Cheng, Hui Wang
Summary: A self-cleaning paper-based microfluidic biosensor was developed using hydrophobic paper decorated with polymeric octadecyl trichlorosilane. The biosensor showed improved performance and could effectively measure the concentration of copper ions in livestock feed and manure.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Yanling Zhang, Jie Wang, Min Liu, Yun Ni, Yao Yue, Dawei He, Ruijiang Liu
Summary: An innovative electrochemical biosensor has been developed for sensitive detection of HER2 protein, an important tumor marker for breast cancer diagnosis and treatment evaluation. The biosensor utilizes specific recognition probes and nanomaterials, along with a unique sensing strategy, to achieve ultra-low detection limit and demonstrate excellent selectivity, reproducibility, and stability.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Humayun Amir, Vasanth Subramanian, Sundaram Sornambikai, Nagamony Ponpandian, Chinnusamy Viswanathan
Summary: In this study, the electrochemical detection of breast cancer biomarker HER2 was successfully achieved using the N-CQDs/GS as the detector. The BSA-modified HER2 Antibody/N-CQDs/GS immunoelectrode preserved excellent activity for the biosensor, while the GS electrode provided high stability and conductivity. The method exhibited a low detection limit and optimal specificity, stability, and reproducibility for detecting HER-2 protein in untreated blood samples from breast cancer patients.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Ilona Uzieliene, Anton Popov, Raminta Vaiciuleviciute, Gailute Kirdaite, Eiva Bernotiene, Almira Ramanaviciene
Summary: This review discusses the application of polypyrrole (Ppy), an electroconductive polymer, in cell culture experiments with electrical stimulation (ES) and its potential for tissue regeneration. Ppy can direct ES to cells and stimulate differentiation towards different cell lineages. Different types of Ppy materials and their combination with active molecules are explored.
BIOELECTROCHEMISTRY
(2024)
