Journal
AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL
Volume 226, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.autneu.2020.102672
Keywords
Bladder physiology; Hypogastric nerve; Saphenous nerve; Electrical nerve stimulation; Rat; Intravesical acetic acid
Categories
Funding
- Canadian Institutes of Health Research (CIHR) Proof of Principle grant [345331]
- University of Toronto Connaught Innovation Award
- Canada Foundation for Innovation (John R Evans Leaders Fund) [31506]
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Saphenous (SAFN) nerve stimulation was recently shown in anesthetized rats to elicit bladder-inhibitory responses in a frequency-dependent manner; however, the mechanism of action is unknown. The goal of this study was to investigate the potential role of the hypogastric nerve (HGN) in this inhibitory pathway by examining stimulation-evoked changes in bladder function under four different experimental conditions: (1) HGN intact, saline infusion (HGNi-s), (2) HGN transected, saline infusion (HGNt-s), (3) HGN intact, acetic acid (AA) infusion (HGNi-a), and (4) HGN transected, AA infusion (HGNt-a). Experiments were conducted in 33 urethane-anesthetized female rats, where continuous bladder infusion was provided through a suprapubic catheter. The experimental protocol involved two, 40-min stimulation trials in which electrical pulses were applied to the SAFN at a set frequency (10 Hz) and two different amplitudes (50 mu A and 100 mu A). In all experimental groups, SAFN stimulation resulted in complete suppression of bladder activity with an incidence rate of 25% to 50%. However, significant changes in the measured urodynamic changes (e.g., basal pressure, contraction amplitude, and inter-contraction interval) were found only in the HGNt-a animals. Our findings suggest that the HGN does not mediate the inhibitory effects of SAFN stimulation and that bladder inhibition is achieved through a different mechanism of action.
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