Article
Biochemistry & Molecular Biology
Simon Schloer, Michael Pflaum, Klaus Hoeffler, Christian Kuehn, Axel Haverich, Bettina Wiegmann
Summary: Lung transplantation is the only curative therapy for end-stage lung diseases, but it is limited to selected patients. Researchers have developed an implantable biohybrid lung based on hollow fiber membrane technology to provide an alternative treatment option. The hemocompatibility of the lung's blood contacting surfaces, achieved through endothelialization, is crucial for its durability. In vitro investigations using human endothelial cells have shown feasibility, and now the appropriate porcine in vivo model needs to be established to bridge the translational gap. The isolated porcine endothelial cells from carotid arteries have been used for endothelialization and demonstrated good viability, resistance to flow conditions, and improved hemocompatibility. The behavior of the porcine endothelial cells is comparable to human endothelial cells, supporting the further examination of the biohybrid lung in pre-clinical studies using porcine lung injury models.
Article
Biochemistry & Molecular Biology
Michael Pflaum, Sophie Jurmann, Katherina Katsirntaki, Marisa Maelzer, Axel Haverich, Bettina Wiegmann
Summary: In this study, we aimed to develop a biohybrid lung using hollow fiber membrane technology as a treatment option for patients with end-stage lung disease. We found that a fibronectin coating on the membrane surface improved endothelial cell adherence and viability, as well as hemocompatibility compared to the commonly used albumin/heparin coating.
Article
Biotechnology & Applied Microbiology
Hussam Almesto Alabdullh, Michael Pflaum, Marisa Maelzer, Marcel Kipp, Hossein Naghilouy-Hidaji, Denise Adam, Christian Kuehn, Russlan Natanov, Adelheid Niehaus, Axel Haverich, Bettina Wiegmann
Summary: In order to establish a long-term lung-assist device for both bridge and destination therapy, a biohybrid lung (BHL) was developed based on contemporary extracorporeal membrane oxygenation (ECMO). The main challenge was to endothelialize all blood-contacting areas to overcome the drawbacks of ECMO. Through experimental testing, it was found that by adjusting cell seeding density and distribution, viable and confluent endothelial cell monolayers were successfully formed within 24 hours, even on different polymer surfaces.
BIOENGINEERING-BASEL
(2023)
Article
Chemistry, Analytical
Michael Pflaum, Julia Dahlmann, Lena Engels, Hossein Naghilouy-Hidaji, Denise Adam, Janina Zoellner, Annette Otto, Sabrina Schmeckebier, Ulrich Martin, Axel Haverich, Ruth Olmer, Bettina Wiegmann
Summary: The study demonstrated the potential therapeutic effect of iPSC-derived endothelial cells (ECs) in forming a continuous, non-thrombogenic, and non-inflammatory monolayer on blood-contacting surfaces of biohybrid lung (BHL). These specific ECs could reduce platelet adhesion and repopulate scratched areas, showing promise for BHL application.
Review
Engineering, Biomedical
Yiming Ma, Lei Gao, Yunqing Tian, Pengguang Chen, Jing Yang, Lei Zhang
Summary: Cell preservation technologies, including hypothermic preservation and cryopreservation, play a critical role in cell-based applications. This review provides an overview of the background, development, and cellular protective mechanisms of these technologies, as well as discusses the advanced biomaterials used in cell preservation.
ACTA BIOMATERIALIA
(2021)
Article
Physics, Applied
Marcelo Saito Nogueira, Michael Raju, Katarzyna Komolibus, Konstantin Grygoryev, Stefan Andersson-Engels
Summary: The study investigated the effect of temperature on tissue microstructural and biochemical parameters during cold preservation of mouse organs, extracting various tissue parameters such as blood oxygenation and scattering amplitude. These findings can be used to propose new strategies to minimize cellular and tissue damage.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Zhengyu Piao, Jin Kyung Park, So-Jung Park, Byeongmoon Jeong
Summary: A polypeptide-based thermogel has been developed for hypothermic storage of stem cells at ambient temperature, showing higher cell recovery rate and healthier stem cell status compared to traditional preservation solutions. Membrane stabilization is suggested as a cell protection mechanism in the cytocompatible thermogel, offering a promising tool for cell transportation and short-term banking.
Review
Biochemistry & Molecular Biology
Raquel G. Bardallo, Rui T. da Silva, Teresa Carbonell, Carlos Palmeira, Emma Folch-Puy, Joan Rosello-Catafau, Rene Adam, Arnau Panisello-Rosello
Summary: This review examines the effects of preservation methods on mitochondrial functions in marginal liver grafts and discusses various preservation solutions and additives to protect mitochondrial machinery. The goal is to increase the pool of suitable organs and improve transplant outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
Laura W. D. Knijff, Cees van Kooten, Rutger J. Ploeg
Summary: Hypothermic machine perfusion (HMP) is the new gold standard for clinical kidney preservation and a promising strategy for higher risk donor livers. HMP has been shown to decrease the risk of delayed graft function (DGF) and improve graft survival in kidney transplantation. HMP prior to liver transplantation may reduce the chance of early allograft dysfunction (EAD) and reduce ischemic damage in the biliary tract.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Surgery
Cyonna Holmes, Ryan J. Vela, LaShondra Powell, Sarah Brant, Matthias Peltz, Michael E. Jessen
Summary: The study found that during hypothermic machine perfusion, the heart prefers to oxidize acetate and octanoate, while beta-hydroxybutyrate is a minor substrate. TCA cycle intermediate concentrations were higher in groups perfused with substrates compared to those without substrates, indicating the necessity of an exogenous, oxidizable substrate to support metabolism in the cold perfused heart. An anaplerotic substrate is not essential to maintain the TCA cycle intermediate pool and support oxidative metabolism under these conditions.
JOURNAL OF SURGICAL RESEARCH
(2021)
Article
Immunology
Yuanjia Zhu, Yasuhiro Shudo, Hao He, Joo Young Kim, Stefan Elde, Kiah M. Williams, Sabrina K. Walsh, Tiffany K. Koyano, Brandon Guenthart, Y. Joseph Woo
Summary: This study compared the outcomes of heart transplantation using the SherpaPak system versus the conventional static cold storage method. The results showed that SherpaPak patients had similar postoperative outcomes but required fewer transfusions. Therefore, adopting the SherpaPak system to accept organs from remote locations may be a viable option to expand the donor pool.
Article
Nanoscience & Nanotechnology
Kun Liu, Lei Wang, Dongdong Li, Shaoduo Yan, Jiayao Li, Xiaoyang Yi, Yunfeng Sun, Yanhong Li, Xuan Zhang, Fengying Qi, Yizhe Zheng, Zixin He, Donggen Wang, Yuyuan Ma, Jun Liang, Qiuxia Fu
Summary: By mimicking the in vivo extracellular matrix, a peptide scaffold has been shown to enhance the storage efficiency of stem cells under hypothermic conditions, preserving their viability and functionality. This provides a feasible and convenient preservation system for stem cells, potentially promoting the clinical application of hematopoietic stem cell therapy.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Biochemistry & Molecular Biology
Maryne Lepoittevin, Sebastien Giraud, Thomas Kerforne, Benoit Barrou, Lionel Badet, Petru Bucur, Ephrem Salame, Claire Goumard, Eric Savier, Julien Branchereau, Pascal Battistella, Olaf Mercier, Sacha Mussot, Thierry Hauet, Raphael Thuillier
Summary: Organ transplantation is the last resort for treating organ failure, but the shortage of organs remains a challenge. To address this issue, there is a need to expand the donor pool and optimize preservation conditions to improve the quality of transplanted organs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Bao Tran Duy Nguyen, Hai Yen Nguyen Thi, Bich Phuong Nguyen Thi, Dong-Ku Kang, Jeong E. Kim
Summary: The COVID-19 pandemic in 2020 highlighted the importance of artificial lung membrane technology, while the aging global population is leading to a higher demand for better artificial organ devices. Despite significant advancements in AO technology over the past decades, the quality of life for organ failure patients remains poor, emphasizing the need for further improvements, particularly in membrane technology. The research on membrane development for AO devices is relatively limited, likely due to the interdisciplinary nature of AO technology, ranging from biotechnology to polymer chemistry and process engineering.
Article
Biotechnology & Applied Microbiology
Claudia Hernandez-Jimenez, J. Raul Olmos-Zuniga, Matilde Baltazares-Lipp, Rogelio Jasso-Victoria, Adrian Polo-Jerez, Maria Teresa Perez-Lopez, Luis Florentino Vazquez-Justiniano, Nestor Emmanuel Diaz-Martinez, Miguel Gaxiola-Gaxiola, Laura Romero-Romero, Axel Edmundo Guzman-Cedillo, Mario Enrique Baltazares-Lipp, Juan Carlos Vazquez-Minero, Luis Horacio Gutierrez-Gonzalez, Marcelino Alonso-Gomez, Mariana Silva-Martinez
Summary: This study investigated the quantification of ECE-1 and VEGF in a swine model of EVLP with standard hypothermic preservation. The results showed that ECE-1 levels remained constant over time, while VEGF levels were initially higher after prolonged preservation but decreased during EVLP. Additionally, parameters such as hemodynamics, gas exchange, mechanical ventilation, and histology tended to improve after 12 hours of hypothermic preservation in the delayed initiation group.
Article
Biology
Vitalii Mutsenko, Sven Knaack, Lothar Lauterboeck, Dmytro Tarusin, Bulat Sydykov, Ramon Cabiscol, Dmitrii Ivnev, Jan Belikan, Annemarie Beck, Daniele Dipresa, Anja Lode, Thaqif El Khassawna, Marian Kampschulte, Roland Scharf, Alexander Yu. Petrenko, Sotirios Korossis, Willem F. Wolkers, Michael Gelinsky, Birgit Glasmacher, Oleksandr Gryshkov
Article
Biochemistry & Molecular Biology
Sabra Zouhair, Eleonora Dal Sasso, Sugat R. Tuladhar, Catia Fidalgo, Luca Vedovelli, Andrea Filippi, Giulia Borile, Andrea Bagno, Massimo Marchesan, Giorgio De Rossi, Dario Gregori, Willem F. Wolkers, Filippo Romanato, Sotirios Korossis, Gino Gerosa, Laura Iop
Article
Cell & Tissue Engineering
Panagiotis Mallis, Michalis Katsimpoulas, Alkiviadis Kostakis, Daniele Dipresa, Sotiris Korossis, Aggeliki Papapanagiotou, Eva Kassi, Catherine Stavropoulos-Giokas, Efstathios Michalopoulos
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2020)
Article
Multidisciplinary Sciences
Raffaele Brogna, Harriette Oldenhof, Harald Sieme, Constana Figueiredo, Tobias Kerrinnes, Willem F. Wolkers
Article
Biochemistry & Molecular Biology
Denys Pogozhykh, Dorothee Eicke, Oleksandr Gryshkov, Willem E. Wolkers, Kai Schulze, Carlos A. Guzman, Rainer Blasczyk, Constanca Figueiredo
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Juergen Lotz, Sercan Icli, Dejia Liu, Suekrue Caliskan, Harald Sieme, Willem F. Wolkers, Harrieette Oldenhof
Summary: The study examined the permeation kinetics of different cryoprotective agents in equine oocytes and ovarian tissue, revealing variations in membrane permeability. These findings provide valuable insights for the rational design of cryopreservation strategies.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Spectroscopy
Sukru Caliskan, Harriette Oldenhof, Raffaele Brogna, Bita Rashidfarokhi, Harald Sieme, Willem F. Wolkers
Summary: This study evaluated the use of FTIR as a non-invasive method to assess oxidative stress-related changes in biomolecules and tissues. The results showed that FTIR combined with PCA can be used to study oxidative damage, but sample heterogeneity in tissues makes it difficult to detect early signs of damage.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Engineering, Biomedical
Claudia Neunaber, Catharina Dalinghaus, Katrin Bundkirchen, Sotiria Toumpaniari, Luisa Marilena Gladitz, Akram Joda, Lucrezia Morticelli, Christian Krettek, Sotirios Korossis
Summary: The study aimed to develop a mild, enzyme-free chemical decellularization method while preserving the biomechanical properties of cartilage. Through experiments on porcine osteochondral tissue, it was found that freezing treatment could effectively decellularize the superficial and middle zones, but not the deep zone. Biomechanical analysis showed that decellularization had an impact on the elastic modulus, but not on other parameters.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Marvin Steitz, Sabra Zouhair, Mahamuda Badhon Khan, Alexander Breitenstein-Attach, Katharina Fritsch, Sugat Ratna Tuladhar, Dag Wulsten, Willem-Frederik Wolkers, Xiaolin Sun, Yimeng Hao, Jasper Emeis, Hans-E. Lange, Felix Berger, Boris Schmitt
Summary: A novel alternative crosslinking method was investigated for collagen-based biomaterials, showing improved thermal and enzymatic stability compared to glutaraldehyde, while maintaining similar biomechanical properties. The study suggests potential for the novel crosslinking agent in intraoperative processing of biomaterials, with further long-term studies required for assessing longevity and calcification.
BIOENGINEERING-BASEL
(2023)
Article
Biology
Charles A. Elder, Jensen S. Smith, Mustafa Almosawi, Ethan Mills, Brett R. Janis, Jonathan A. Kopechek, Willem F. Wolkers, Michael A. Menze
Summary: By using a biomimetics approach and trehalose as a cryoprotective agent, red blood cells can be successfully preserved for long-term storage without the need for time-consuming washing steps.
Article
Biology
Alexandra Roesch, Roland Windisch, Christian Wichmann, Willem F. Wolkers, Gideon Kersten, Tim Menzen
Summary: In this study, a novel cell sizing technique called flow imaging microscopy (FIM) was used to assess the osmotic response of T cells. The findings were corroborated with electrical impedance measurements conducted on a Coulter counter. It was found that FIM combined with Trypan blue staining could accurately estimate cell volume parameters and differentiate between viable and dead cells. The study also revealed that membrane permeability to water and cryoprotective agents decreased with decreasing temperature, with Me2SO permeating into the cells faster than glycerol.
Article
Engineering, Biomedical
Cristian C. D'Alessandro, Andreas Dimopoulos, Sofia Andriopoulou, Gerasimos A. T. Messaris, Sotirios Korossis, Petros Koutsoukos, Dimosthenis Mavrilas
Summary: This study presents a comparative methodology for investigating calcium phosphate deposits on different heart valve prostheses in vitro. It was found that decellularized porcine aortic valves showed slightly higher rates of calcification compared to glutaraldehyde-fixed ones, attributed to tissue modifications from the decellularization process. Octacalcium phosphate crystallites were preferentially deposited in high mechanical stress areas, indicating the importance of mechanical forces in tissue mineralization. The in vitro circulatory model developed in this study serves as a valuable pre-screening tool for understanding the calcification process of bioprosthetic and tissue-engineered valves under physiological mechanical load.
BIO-DESIGN AND MANUFACTURING
(2021)