Article
Biology
A. N. Omelchenko, T. N. Igonina, E. Y. Brusentsev, K. A. Okotrub, S. Y. Amstislavsky, N. V. Surovtsev
Summary: Cryopreservation affects fatty acid accumulation in embryos, with different effects at different developmental stages.
Article
Biochemistry & Molecular Biology
Pradjna N. Paramitha, Riki Zakaria, Anisa Maryani, Yukako Kusaka, Bibin B. Andriana, Kosuke Hashimoto, Hiromitsu Nakazawa, Satoru Kato, Hidetoshi Sato
Summary: The study revealed that oleic and linoleic acids promote higher rates of fat accumulation in hepatic cells compared to palmitic and stearic acids, with linoleic acid causing cell death while oleic acid does not. Additionally, linoleic fatty chains taken into the cells are modified into oleic fatty chains, suggesting a potential role in inflammation in nonalcoholic fatty liver disease (NAFLD).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Plant Sciences
Yingqi Cai, Xiao-Hong Yu, John Shanklin
Summary: Plants convert carbohydrates into energy-dense lipids through photosynthesis, making plant oils an economically and sustainably attractive feedstock for biofuels and value-added bioproducts. Various strategies have been developed to enhance lipid accumulation and produce specialty lipids with increased uses and value. Successful metabolic engineering involves expression of lipogenic factors from other sources or with specialized functionality.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Plant Sciences
Changcheng Xu, Jilian Fan
Summary: This review discusses the metabolic interplay between autophagy and lipid droplet dynamics, mechanisms of lipophagy, and cellular management of lipotoxic stress during autophagy. Autophagy degrades membrane lipids to provide fatty acids for energy production, while lipid droplets can be degraded via lipophagy in times of energy scarcity. However, lipid droplets are also necessary for efficient autophagic processes, and cells must carefully manage the cytotoxic effects of free fatty acids during autophagy-mediated mobilization for energy generation.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Spectroscopy
Konstantin A. Okotrub, Daria Shamaeva, Nikolay Surovtsev
Summary: Raman spectroscopy has been employed to study the composition of cell lipidome, but its limited distinguish power for different lipids has been an issue. Recently, isotopically labeled molecules have been used to enhance the Raman resolution of similar compounds in biological samples. Sensitivity of Raman peaks to order-disorder transition corresponding to isotopically shifted Raman peaks was demonstrated, providing information about the orientation of deuterated Raman tags and their surroundings.
JOURNAL OF RAMAN SPECTROSCOPY
(2022)
Review
Cell Biology
Mauro Danielli, Leja Perne, Eva Jarc Jovicic, Toni Petan
Summary: Lipid droplets are organelles that store fat and play a critical role in regulating lipid metabolism. They undergo a continuous turnover process of biogenesis and breakdown to meet the cell's needs for fatty acids, including polyunsaturated fatty acids. Precise regulation of lipid droplet turnover is crucial for maintaining the distribution and homeostasis of polyunsaturated fatty acids.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Reproductive Biology
Malgorzata Ochota, Michal Jerzy Kulus, Wieslawa Mlodawska, Marta Kardasz-Kamocka, Katarzyna Haczkiewicz-Lesniak, Marzenna Podchorska-Okolow, Wojciech Nizanski
Summary: Despite the established microscopic markers of feline oocyte quality, there is limited knowledge on their ultrastructural traits, particularly the effects of ovarian storage time. This study examined the ultrastructural characteristics of cat oocytes after 24 and 48 hours of storage using transmission electron microscopy (TEM). It was found that good quality oocytes showed more easily identifiable and obvious distribution patterns of cytoplasmic organelles compared to bad quality ones. Additionally, the area occupied by cytoplasmic vacuoles was significantly larger in good quality oocytes from fresh ovaries, while lipid droplets occupied more cytoplasm in bad quality and stored oocytes. These findings highlight the importance of TEM in assessing ultrastructure changes in stored feline oocytes. Further investigations with larger sample sizes are needed to confirm these observations.
Article
Surgery
Shin-Hoo Park, Seong-Woo Bae, Kyoung-Yun Jeong, Eun-Hee Koo, Jong-Ho Choi, Ji-Hyeon Park, Seong-Ho Kong, Won-Sil Choi, Do Joong Park, Hyuk-Joon Lee, Han-Kwang Yang
Summary: This study found that the droplet particles in the peritoneal fluid after laparoscopic gastric cancer surgery contained various types of fatty acids, some of which were found to be associated with inflammatory response.
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES
(2022)
Article
Biochemistry & Molecular Biology
Jingjing Tian, Yihui Du, Binbin Wang, Mengmeng Ji, Hongyan Li, Yun Xia, Kai Zhang, Zhifei Li, Wenping Xie, Wangbao Gong, Ermeng Yu, Guangjun Wang, Jun Xie
Summary: Excessive hepatic lipid accumulation in cultured fish is poorly understood. In this study, using a zebrafish liver cell line, the authors found that LD accumulation is accompanied by the differential expression of LD-related genes, including dhrs3a/b. Knockdown of dhrs3a delayed LD accumulation and downregulated pparg expression. Dhrs3 catalyzed retinene to retinol, which increased in LD-enriched cells. The addition of exogenous retinyl acetate maintained LD accumulation and altered the lipidome of the cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yuchuan Li, May-Kristin Torp, Frode Norheim, Prabhat Khanal, Alan R. Kimmel, Kare-Olav Stenslokken, Jarle Vaage, Knut Tomas Dalen
Summary: Plin5 is abundantly expressed in the heart where it facilitates the interaction between lipid droplets and mitochondria. Its role in fatty acid uptake and oxidation in cardiomyocytes was studied, revealing that Plin5 affects lipid droplet storage primarily by repressing adipose triglyceride lipase activity. Interestingly, Plin5 expression and cardiac lipid droplet content have little impact on tolerance to acute hypoxia and ischemia, contrasting its protective role in mouse models during myocardial ischemia.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Immunology
Annette L. L. West, Johanna von Gerichten, Nicola A. A. Irvine, Elizabeth A. A. Miles, Karen A. A. Lillycrop, Philip C. C. Calder, Barbara A. A. Fielding, Graham C. C. Burdge
Summary: This study found that lipid metabolism in human T lymphocytes changes during the life course, which may facilitate the adaptation of immune function to different stages of life.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Analytical
Dan-Ying Ma, Juan-Juan Fu, Jin-Lan Tang, Yi-Lin Bao, Lin-Wei Shang, Xiao Wang, Jian-Hua Yin
Summary: The study used Raman microspectroscopy to compare the spectral intensity of healthy and cancerous breast tissues, revealing changes in lipid and protein content during the process of canceration. Specifically, saturated fatty acids were converted into unsaturated fatty acids, and the content of polyunsaturated fatty acids increased while monounsaturated fatty acids decreased. These findings are significant for understanding the biochemical mechanism of breast cancer and can aid in early monitoring and diagnosis through spectroscopic techniques.
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
(2021)
Article
Oncology
Yiran Zhou, Xiao Li, Aoran Guan, Haodong Zhou, Yankun Zhu, Ruotian Wang, Ruhong Li
Summary: Tumor cells use metabolic reprogramming to meet the energy and balance needed for rapid tumor division, but the exact role of fatty acid metabolism in CRC is limited. In this study, the tumor suppressor gene EPHX2 was found to be associated with the inhibitory effect of fatty acid degradation in CRC through data analysis and experiments.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cell Biology
Triana Amen, Daniel Kaganovich
Summary: Metabolic regulation is essential for stress response pathways, and the interconnection between stress granule formation and lipid droplet accumulation has been uncovered, revealing a novel relationship between stress adaptation mechanisms.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Simon Arhar, Gabriela Gogg-Fassolter, Mojca Ogrizovic, Klavdija Pacnik, Katharina Schwaiger, Mia Zganjar, Uros Petrovic, Klaus Natter
Summary: By combining various mutations, the typically non-TAG-accumulating baker's yeast can be converted into a highly TAG-rich strain, highlighting the importance of strain selection and metabolic engineering for achieving high product levels.
MICROBIAL CELL FACTORIES
(2021)
Article
Instruments & Instrumentation
Sergey V. Adichtchev, Konstantin A. Okotrub, Alexey M. Pugachev, Irina V. Zaytseva, Nikolay V. Surovtsev
Summary: This study investigated binary phospholipid bilayers composed of DPPC using Raman spectroscopy and DSC, finding that certain features in Raman spectra can accurately indicate lipid phase and phase coexistence. It was discovered that the spectral characteristics of specific Raman lines can be used to detect ordered and disordered lipid phases coexisting within the DPPC concentration range. The results provide a robust label-free contactless approach for the detection of lipid phase separation with micrometer resolution.
APPLIED SPECTROSCOPY
(2021)
Article
Behavioral Sciences
Anna V. Klenova, Ilya A. Volodin, Elena V. Volodina, Svetlana V. Ranneva, Tamara G. Amstislavskaya, Tatiana V. Lipina
Summary: This study identified a new acoustic phenotype in Calsyntenin2 deficient mice during the neurodevelopment period, showing differences in ultrasonic calls and increased head-to-body ratio. Lack of calsyntenin2 affected vocalization in mice, with higher call rates in certain discomfort conditions. The emission of wideband ultrasonic calls remained consistent as a reliable indicator in Clstn2-KO mice.
BEHAVIOURAL BRAIN RESEARCH
(2021)
Article
Biophysics
Konstantin A. Okotrub, Svetlana Okotrub, Valentina Mokrousova, Sergei Y. Amstislavsky, Nikolay Surovtsev
Summary: Raman spectroscopy of deuterium-labeled lipids was used to study the freezing of lipid droplets inside cat oocytes, revealing lipid phase transitions and redistributions.
BIOPHYSICAL JOURNAL
(2021)
Article
Chemistry, Applied
Petr M. Yeletsky, Yury V. Dubinin, Nikolay A. Yazykov, Roman B. Tabakaev, Konstantin A. Okotrub, Vadim A. Yakovlev
Summary: The study investigated the development of porosity in porous carbons from vegetable biomass through the leaching of mineral phases from the carbonized feedstock. The research found that the method of removing mineral template phases via acid leaching can result in porous carbons with high porosity, with biomass feedstocks showing more effective porosity development.
FUEL PROCESSING TECHNOLOGY
(2022)
Article
Spectroscopy
Konstantin A. Okotrub, Daria Shamaeva, Nikolay Surovtsev
Summary: Raman spectroscopy has been employed to study the composition of cell lipidome, but its limited distinguish power for different lipids has been an issue. Recently, isotopically labeled molecules have been used to enhance the Raman resolution of similar compounds in biological samples. Sensitivity of Raman peaks to order-disorder transition corresponding to isotopically shifted Raman peaks was demonstrated, providing information about the orientation of deuterated Raman tags and their surroundings.
JOURNAL OF RAMAN SPECTROSCOPY
(2022)
Review
Chemistry, Multidisciplinary
Mikhail Yu. Ivanov, Nikolay V. Surovtsev, Matvey V. Fedin
Summary: This article provides a systematic description of the properties and applications of ionic liquids in the glassy state, which has not been published previously. It is of significant importance for innovations in organic synthesis, energy storage, and cryopreservation.
RUSSIAN CHEMICAL REVIEWS
(2022)
Article
Chemistry, Physical
Dmitrij A. Belousov, Kirill A. Bronnikov, Konstantin A. Okotrub, Sergey L. Mikerin, Victor P. Korolkov, Vadim S. Terentyev, Alexander V. Dostovalov
Summary: Thermochemical laser-induced periodic surface structures (TLIPSS) are periodic structures formed in the focal area of linear polarized laser radiation by the thermally stimulated reaction of oxidation, which have the potential to alter the optical and physical properties of materials surfaces. Experimental results on TLIPSS formation at different ambient air pressures showed that high-ordered TLIPSS can still be formed within a wide range of parameters variation, despite the decrease in oxygen content.
Article
Instruments & Instrumentation
Valeria A. Zykova, Nikolay Surovtsev
Summary: In this study, Brillouin spectroscopy was used to investigate the spatial organization of multicomponent lipid bilayers, revealing the existence of phase domains on a scale of a hundred nanometers. The results showed that lateral phonons are preferable for testing the lateral homogeneity of the bilayers, while phonons spreading across the bilayers are sensitive to the layered packing.
APPLIED SPECTROSCOPY
(2022)
Article
Chemistry, Physical
E. A. Dobrynina, V. A. Zykova, S. V. Adichtchev, N. V. Surovtsev
Summary: This study investigated the possible relationship between the macroscopic viscosity changes in salt solutions and their microscopic dynamic response. It was found that Jones-Dole's B-coefficient correlates linearly with the average OH stretching frequency, real and imaginary parts of gigahertz elastic modulus. Additionally, it was discovered that structure-making ions not only change the frequency of the relaxation peak but also increase the low-frequency part of the relaxation susceptibility. However, the isotropic Raman contribution associated with the tetrahedral-like environment of water molecules does not have a significant influence on viscosity.
JOURNAL OF CHEMICAL PHYSICS
(2023)
Article
Engineering, Electrical & Electronic
Anton S. Sinko, Nikolay V. Surovtsev, Aleksey V. Kargovsky, Nazar A. Nikolaev, Vera L. Manomenova, Natalia N. Kozlova, Elena B. Rudneva, Alexey E. Voloshin, Alexander P. Shkurinov
Summary: This article studies narrow-band terahertz (THz) emission stimulated by femtosecond laser pulse in molecular crystal GUHP. The authors demonstrate the close connection between this emission and the excitation of high-quality phonon oscillations in the crystal through temperature dynamics of the spectra and DFT calculations. They also analyze the polarization sensitive spectra and THz transmission spectra to understand the origin of this stimulated THz emission and develop an adequate model for the phenomenon.
IEEE TRANSACTIONS ON TERAHERTZ SCIENCE AND TECHNOLOGY
(2023)
Article
Spectroscopy
Anastasia N. Omelchenko, Konstantin A. Okotrub, Nikolay V. Surovtsev
Summary: Raman spectroscopy of cells cultured in a deuterated substrate shows promise for studying mass transfer and enzymatic reactions. In this study, we investigated the potential of this approach using yeast cells cultured under aerobic and anaerobic conditions. Our results indicate that adding heavy water leads to a decrease in lipid content in cells and additional Raman peaks are observed in cells cultured at high concentrations of heavy water. We also observed a correlation between the deuteration of ethanol synthesized by yeast fermentation and the concentration of heavy water. These findings qualitatively agree with the known estimate that aerobic metabolism is 15 times more active than ethanol fermentation. This work establishes new limitations and prospects for the application and development of Raman spectroscopy with deuterium tags.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Daria Shamaeva, Konstantin A. Okotrub, Nikolay Surovtsev
Summary: Biomimetic phospholipid mixtures are commonly used in studying biological membranes and drug delivery materials. This study proposes an approach using Raman mapping to study phase coexistence in stacked phospholipid bilayers. By analyzing Raman spectra, the chemical composition and conformational state of lipids in different phases can be determined. The study also reveals the effect of hydration on lipid phase separation. This method has the potential to investigate the phase diagrams of phospholipid mixtures under various experimental conditions.
Proceedings Paper
Engineering, Electrical & Electronic
Kirill Bronnikov, Alexander Dostovalov, Konstantin Okotrub, Viktor Korolkov, Sergey Babin
2021 CONFERENCE ON LASERS AND ELECTRO-OPTICS EUROPE & EUROPEAN QUANTUM ELECTRONICS CONFERENCE (CLEO/EUROPE-EQEC)
(2021)
Article
Biochemistry & Molecular Biology
Yuri Nagaoka, Kotone Oshiro, Yuta Yoshino, Toshiyuki Matsunaga, Satoshi Endo, Akira Ikari
Summary: This study investigated the effect of intercellular adhesion molecule CLDN1 on the anticancer drug sensitivity of small-cell lung cancer (SCLC) cells. It was found that overexpression of CLDN1 decreased the sensitivity of SCLC cells to anticancer drugs and enhanced their migratory capacity through the activation of the TGF-81/EMT signaling pathway. Treatment with EMT inhibitors showed potential in overcoming the reduced sensitivity to anticancer drugs in CLDN1-overexpressing SCLC cells.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2024)
