Article
Fisheries
Peng Zhang, Boguang Sun
Summary: This study investigates the involvement of PoCry1 in the immune response during bacterial infection in Japanese flounder. The results show that PoCry1 is involved in anti-infection immunity and exhibits circadian oscillation in transcription upon rhythmic light stimulation. These findings suggest that light condition can be exploited to strengthen fish immune system and benefit disease control in aquaculture.
Article
Multidisciplinary Sciences
Yasuhiro Umemura, Nobuya Koike, Yoshiki Tsuchiya, Hitomi Watanabe, Gen Kondoh, Ryoichiro Kageyama, Kazuhiro Yagita
Summary: Circadian clocks are suppressed during early embryonic stages and pluripotent stem cells in mammals, but gradually emerge during ontogenesis. The expression of CLOCK/BMAL1 affects the oscillation of the segmentation clock, which controls somitogenesis in the early developmental stage.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Editorial Material
Cell Biology
Ueli Schibler
Summary: Mammalian body cells have cell-autonomous and self-sustained circadian oscillators relying on delayed negative feedback loops in gene expression. Transcriptional activation and repression, as well as post-translational mechanisms like phosphorylation and dephosphorylation, play crucial roles in setting the pace of these timekeepers. The study by Klemz and colleagues in Genes & Development demonstrates how dephosphorylation of BMAL1 by protein phosphatase 4 (PPP4) participates in the modulation of circadian timing.
GENES & DEVELOPMENT
(2021)
Article
Cell Biology
Sabrina Klemz, Thomas Wallach, Sandra Korge, Mechthild Rosing, Roman Klemz, Bert Maier, Nicholas C. Fiorenza, Irem Kaymak, Anna K. Fritzsche, Erik D. Herzog, Ralf Stanewsky, Achim Kramer
Summary: In organisms with circadian clocks, the post-translational modifications of clock proteins, particularly phosphorylation, play a crucial role in controlling circadian rhythms. Protein phosphatase 4 (PPP4) and its regulatory subunit PPP4R2 have been identified as critical components in mammals and Drosophila, affecting the circadian system by regulating phosphorylation and transactivation activity of CLOCK/BMAL1.
GENES & DEVELOPMENT
(2021)
Article
Biochemistry & Molecular Biology
Xiaomeng Liu, Niuben Cao, Xinchan Liu, Yu Deng, Yu Xin, Ruobing Fu, Xirui Xin, Yubo Hou, Weixian Yu
Summary: Circadian rhythms play a crucial role in regulating the body's homeostasis, and circadian rhythm disorders can have detrimental effects on metabolic and immune functions. However, the relationship between circadian rhythm disorders and periodontitis has not been well studied. This research aimed to investigate the role of the circadian rhythm control gene BMAL1 in periodontitis. The results showed that downregulation of BMAL1 in circadian rhythm disorder-induced periodontitis led to significant alveolar bone resorption, increased osteoclast differentiation, upregulation of the inflammatory signaling molecule NF-kappa B, elevated apoptosis and oxidative stress levels in periodontal tissues. This study suggests that BMAL1 is a key regulator in periodontitis exacerbated by circadian rhythm disorders, highlighting a potential avenue for periodontitis treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jiayang Zhang, Chengcheng Liu, Qing Liang, Feng Zheng, Youfei Guan, Guangrui Yang, Lihong Chen
Summary: The study revealed a significant role of the core clock gene Bmal1 in tubulointerstitial fibrosis, possibly mediated through its interaction with Gli2.
Article
Biochemistry & Molecular Biology
Sang Ah Yi, Ye Ji Jeon, Min Gyu Lee, Ki Hong Nam, Sora Ann, Jaecheol Lee, Jeung-Whan Han
Summary: This study reveals that S6K1 plays a central role in regulating the production of adiponectin, a hormone derived from fat cells that has beneficial effects on metabolism. S6K1 controls adiponectin expression by inducing a transcriptional switch between two transcriptional machineries, BMAL1 and EZH2. Mechanistically, S6K1 induces a suppressive histone code cascade, leading to suppression of adiponectin expression. Upon fasting, inactivation of S6K1 promotes adiponectin expression by inducing a transcriptional switch from EZH2 to BMAL1. These findings provide insights into the regulatory machinery controlling adiponectin production and may guide the development of treatments for metabolic disorders.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2022)
Article
Medicine, Research & Experimental
Shuai Wang, Yanke Lin, Lu Gao, Zemin Yang, Jingpan Lin, Shujing Ren, Feng Li, Jing Chen, Zhigang Wang, Zhiyong Dong, Pinghua Sun, Baojian Wu
Summary: Growing evidence suggests that circadian clock and obesity are intertwined, but the underlying mechanism is poorly understood. This study investigated the link between circadian clock and obesity, and found that obesity causes perturbance of the circadian clock in white adipose tissue (WAT). Reduced levels of glutamine and methionine in obese WAT were associated with decreased histone acetylation and methylation at the Bmal1 promoter. It was also found that impaired expression of PPAR-gamma in obesity led to downregulation of SLC1A5, resulting in reduced uptake of glutamine and methionine. These findings suggest that PPAR-gamma integrates obesity and adipocyte clock, promoting a vicious cycle between circadian disruption and obesity development.
Article
Biochemistry & Molecular Biology
Weiyun Zhang, Chi-Tang Ho, Muwen Lu
Summary: This study found that piperine improves lipid metabolism disorders in HepG2 cells by regulating the Bmal1 and Clock genes, reducing circadian desynchrony, ROS overproduction, and mitochondrial dysfunction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cardiac & Cardiovascular Systems
Matteo Astone, Roxana E. Oberkersch, Giovanni Tosi, Alberto Biscontin, Massimo M. Santoro
Summary: This study demonstrates that endothelial cells possess an endogenous circadian clock, and core clock genes exhibit robust circadian oscillations. Impairing the function of the circadian clock transcriptional activator BMAL1 leads to angiogenesis defects in both developmental and pathological contexts. BMAL1 regulates endothelial cell cycle progression by binding to the promoters of CCNA1 and CDK1 genes.
CARDIOVASCULAR RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Erica R. Kwiatkowski, Yisrael Schnytzer, Joshua J. C. Rosenthal, Patrick Emery
Summary: Organisms in the intertidal zone face a challenging environment with daily and seasonal changes in light intensity, weather patterns, and tides. To adapt to these conditions, intertidal animals have developed circatidal clocks, but the molecular components have been difficult to identify. This study introduces the crustacean Parhyale hawaiensis as a genetic model to study circatidal rhythms and shows that the core circadian clock gene Bmal1 is required for these rhythms. This research establishes the connection between circatidal and circadian clocks and provides a powerful system to study the molecular mechanisms of circatidal rhythms.
Article
Biochemistry & Molecular Biology
Gene Ryan Crislip, Stephanie E. Wohlgemuth, Christopher A. Wolff, Miguel A. Gutierrez-Monreal, Collin M. Douglas, Elnaz Ebrahimi, Kit-Yan Cheng, Sarah H. Masten, Dominique Barral, Andrew J. Bryant, Karyn A. Esser, Michelle L. Gumz
Summary: The study found that iMS-BMAL1 KO mice exhibited an aging phenotype with no renal phenotype, and their response to low potassium diet and phase advance in renal function was similar to the control group, indicating that disruption of the clock in skeletal muscle does not lead to significant renal injury.
Article
Immunology
Zainab Taleb, Vania Carmona-Alcocer, Kyle Stokes, Marta Haireek, Huaqing Wang, Stephen M. Collins, Waliul I. Khan, Phillip Karpowicz
Summary: Circadian disruption is associated with increased risk of inflammatory bowel disease (IBD), as shown by studies on mice lacking a functional circadian clock. The severity of colitis in these mice is worsened and their recovery impaired. Inflammation, immune cell infiltration, and epithelial cell proliferation in the colon all exhibit daily rhythms, which are disrupted in mice without a functioning circadian clock. These findings highlight the importance of a functional circadian clock in the colon for inflammation and healing.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biology
Vijay K. Jidigam, Onkar B. Sawant, Rebecca D. Fuller, Kenya Wilcots, Rupesh Singh, Richard A. Lang, Sujata Rao
Summary: Research has shown that clock genes are expressed in the mouse embryonic retina, and this expression requires light cues. Deletion of Bmal1 and Per2 from retinal neurons leads to retinal angiogenic defects, indicating that a dysregulated circadian clock primarily drives neovascularization.
COMMUNICATIONS BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Meng Qu, Guoxin Zhang, Han Qu, Alexander Vu, Raymond Wu, Hidekazu Tsukamoto, Zhenyu Jia, Wendong Huang, Heinz-Josef Lenz, Jeremy N. Rich, Steve A. Kay
Summary: Hepatocellular carcinoma (HCC) is a global health challenge with increasing incidence worldwide. The circadian clock has been shown to play a key role in hepatocarcinogenesis, but the cellular and molecular mechanisms underlying the HCC-clock crosstalk are unknown. In this study, we found that HCC cells rely on the circadian clock transcription factors BMAL1 and CLOCK for cell growth, and down-regulation of these factors induces apoptosis and cell cycle arrest. Mechanistically, inhibiting BMAL1/CLOCK dysregulates cell cycle regulators Wee1 and p21, leading to tumor cell death. Our findings provide insights into the cellular impact of clock proteins in HCC oncogenesis and suggest a potential therapeutic approach based on modulation of the circadian clock.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Fisheries
Hunter S. Bailey, Ashley N. Fincannon, Lee A. Fuiman
Summary: This study investigated the transfer of fatty acids from broodstock diets to eggs and the time needed for the eggs to equilibrate to the diet in Southern flounder. The findings indicate that different diet treatments significantly affect the fatty acid composition of eggs, and it takes 8-16 weeks for the eggs to adapt to changes in the adult diet. These results are important for improving broodstock diets and feeding protocols in stock-enhancement programs.
Article
Fisheries
Yu Liu, Mingtao Lei, Hector Victor, Yan Wang
Summary: This study confirms the feasibility of replacing raw fish diet with a formulated diet in commercial farming of large yellow croaker, which can increase fish survival and yield, and reduce feed cost and body lipid deposition.
Article
Fisheries
Xiaoyan Zhang, Lize San, Yucong Yang, Yuehong Tao, Jiangong Ren, Yufeng Liu, Zhongwei He, Jiashuo Di, Ziteng Pei, Guixing Wang, Jilun Hou
Summary: Gynogenesis is an effective technique for establishing homogeneous lines and confirming potential chromosomal mechanisms of sex determination in fish. This study successfully induced gynogenetic families in Thamnaconus modestus and established a complete method for gynogenesis induction.
Article
Fisheries
Julieta C. Martinelli, Megan Considine, Helen R. Casendino, Carolyn M. Tarpey, Isadora Jimenez-Hidalgo, Jacqueline L. Padilla-Gamino, Teri L. King, Lorenz Hauser, Steven Rumrill, Chelsea L. Wood
Summary: Shell-boring polychaetes have caused significant damage to mariculture operations worldwide, particularly in the US Pacific region. This study provides a comprehensive dataset on the prevalence, species distribution, and environmental associations of these parasites, highlighting the impact of season, culturing methods, and environmental factors on infestation.
Article
Fisheries
Juan Gao, Xueshan Li, Kangle Lu, Kai Song, Jian Zhang, Ling Wang, Chunxiao Zhang
Summary: This study investigated the effects of dietary protein levels on growth performance, antioxidant capacity, digestive enzyme activity, and metamorphosis rate of bullfrog tadpoles. The optimal dietary protein level for bullfrog tadpoles was estimated to be 42.49%-46.50% of the diet.
Article
Fisheries
Qiang Li, Lijun Duan, Dongsheng Jin, Yuxin Chen, Yirong Lou, Qianjin Zhou, Zhongjie Xu, Fangjie Chen, Hongxian Chen, Guizong Xu, Maocang Yan, Guanjun Yang, Jianfei Lu, Yanjun Zhang, Jiong Chen
Summary: This study developed a centrifugal microfluidic chip with on-chip RPA to detect five pathogenic microorganisms. The chip enabled the parallel analysis of six genetic markers from a single sample and allowed for the highly automated detection of multiple samples. Compared with PCR and DNA sequencing, the on-chip RPA assay showed high sensitivity and specificity for detecting multiple pathogens in shrimp aquaculture.
Article
Fisheries
Xingchen Huo, Pengxu Wang, Fengxia Zhao, Qian Liu, Qingqing Tian, Lingjie Tang, Maolin Lv, Zhaohui Wei, Chunrong Yang, Jianguo Su
Summary: Bacterial diseases in aquaculture ponds have negative effects on sustainable development. The use of composite I20 biotherapy has been shown to effectively treat bacterial diseases in bullfrog ponds, providing a new strategy for controlling bacterial diseases in aquaculture.
Article
Fisheries
Peng Yin, Takaya Saito, Per Gunnar Fjelldal, Bjorn Tharandur Bjornsson, Sofie Charlotte Remo, Sandeep Sharma, Rolf Erik Olsen, Kristin Hamre
Summary: This study aims to investigate the effect of seasonal changes in temperature and photoperiod on the redox metabolism of Atlantic salmon at the molecular level. The research findings suggest that the changing photoperiod and temperature influenced the redox metabolism of Atlantic salmon, highlighting the importance of oxidative status in the ecological implications.
Article
Fisheries
Nicholas Oppong Mensah, Jeffery Kofi Asare, Emmanuel Tetteh-Doku Mensah, Ernest Christlieb Amrago, Frank Osei Tutu, Anthony Donkor
Summary: This study investigates aquaculture farmers' preference for climate-smart aquaculture insurance products, the challenges they face, and their preferred insurance coverage. The results show that farmers prefer Climate-Induced Aquaculture Stock Mortality Insurance and the most significant constraint is the delay in claim settlement.
Article
Fisheries
Qiu-Ping Chai, Pei Wu, Wei-Dan Jiang, Yang Liu, Hong-Mei Ren, Xiao-Wan Jin, Lin Feng, Xiao-Qiu Zhou
Summary: The study found that appropriate levels of potassium diformate (KDF) can enhance the immune defense and mediate the inflammatory process in fish, possibly through the regulation of T cell differentiation via JAK-STAT and NF-kappa B signaling pathways.
Article
Fisheries
Tian Zhu, Haomin Jia, Haopeng Zhang, Yujing Xiao, Cui Han, Jiaxin Yang
Summary: Chicken manure has significant effects on the cultivation of Chlorella and rotifers. The optimal amount of chicken manure extract for continuous cultivation is 10 mLL-1. Chicken manure can promote the growth of Chlorella and improve the fatty acid composition, indirectly enhancing the growth condition and fatty acid content of rotifers.
Article
Fisheries
Zheng Luo, Yang Yu, Zhenning Bao, Fuhua Li
Summary: This study analyzed the heritability and genetic correlation of two growth traits in Pacific white shrimp and evaluated the genomic prediction using different genomic selection models. The results showed that the NeuralNet model had the highest prediction accuracy and better prospects for predicting shrimp growth traits.
Article
Fisheries
Alberto Ruiz, Ignasi Sanahuja, Karl B. Andree, Dolors Furones, Paul G. Holhorea, Josep A. Calduch-Giner, Jose J. Pastor, Marc Vinas, Jaume Perez-Sanchez, Sofia Morais, Enric Gisbert
Summary: Supplementing diets with a combination of spice oleoresins can reduce lipid accumulation and inflammation in farmed fish. The combination of spice oleoresins also has an immunomodulatory effect on the fish's intestinal immune system, potentially offering anti-inflammatory benefits.
Article
Fisheries
Beibei Zhao, Renhui Mei, Le Li, Di Hu, Lei Li
Summary: This study reveals that JfCXCL8_L1a and JfCXCL8_L1b have different immune pathways, and JfCXCL8_L1b plays a significant role in enhancing the adaptive immunity of T cell-dependent antigen.
Article
Fisheries
Yuhang Liu, Danying Cao, Nan Wu, Xuyang Zhao, Qingsong Zhu, Lian Su, Fatima Altaf, Qianqian Zhang, Haokun Liu, Yongming Li, Bruno Hamish Unger, Yingyin Cheng, Wanting Zhang, Aihua Li, Yaping Wang, Xiao-Qin Xia
Summary: Based on previous research, sinomenine has been found to protect mucosal immunity in farmed fish species by preventing intestinal pathological changes and regulating gene expression related to inflammation. It also enhances immune homeostasis and controls the growth of pathogenic bacteria.