Editorial Material
Hematology
John Strouboulis, Antonella E. Ronchi
Summary: In this article, Ludwig et al characterize a rare form of inherited hemolytic anemia and identify novel mutations in an intrinsically disordered region (IDR) in the GATA1 carboxyl (C)-terminal domain. These mutations have novel functions in selectively regulating GATA1 chromatin occupancies in erythropoiesis.
Editorial Material
Hematology
Julie A. Thoms, John E. Pimanda, Olaf Heidenreich
Summary: These articles elucidate the complex regulation of PU.1 gene expression, involving the interaction of an enhancer with canonical sense and alternate antisense promoters.
Article
Nanoscience & Nanotechnology
Pawan Kumar Raghav, Gurudutta Gangenahalli
Summary: The study demonstrates that the synthetic PU.1 β3/β4 mimic peptides and peptide-loaded PLGA nanoparticles can enhance the expression of myeloid markers and increase granulocyte production, suggesting a potential to boost hematopoietic stem cell commitment to myeloid cells in the context of opportunistic infections.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Immunology
Hiroyuki Hosokawa, Maria Koizumi, Kaori Masuhara, Maile Romero-Wolf, Tomoaki Tanaka, Toshinori Nakayama, Ellen Rothenberg
Summary: The transcription factors Runx1 and GATA3 have been found to regulate Spi1 expression levels in pro-T cells, controlling the repression of Spi1 during T lineage commitment. This regulation is achieved through a specific intronic silencing element in mouse Spi1.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Shin'ya Ohmori, Jun Takai, Satoshi Uemura, Akihito Otsuki, Tetsuya Mori, Kinuko Ohneda, Takashi Moriguchi
Summary: Inflammation-dependent cytokine production in mast cells is regulated by the collaborative activity of GATA2 and PU.1 through their overlapping binding sites in distal cis-regulatory regions.
Article
Cell Biology
Maaike R. Scheenstra, Patricia Martinez-Botia, Andrea Acebes-Huerta, Rutger W. W. Brouwer, Noemi Caballero-Sanchez, Nynke Gillemans, Pieter De Bleser, Benjamin Nota, Iris M. De Cuyper, Vishal Salunkhe, Andrea M. Woltman, Lianne Laar, Erikjan Rijkers, Jeroen A. A. Demmers, Wilfred F. J. IJcken, Sjaak Philipsen, Timo K. den Berg, Taco W. Kuijpers, Laura Gutierrez
Summary: Dendritic cells (DCs) play a crucial role in immune regulation, with the transcription factor PU.1 being identified as a key player in the biology of DCs. Study findings reveal significant interspecies differences between human and mouse DCs, underscoring the importance of studying human DC biology.
JOURNAL OF LEUKOCYTE BIOLOGY
(2021)
Article
Biology
Xinhui Zhao, Boris Bartholdy, Yukiya Yamamoto, Erica K. Evans, Meritxell Alberich-Jorda, Philipp B. Staber, Touati Benoukraf, Pu Zhang, Junyan Zhang, Bon Q. Trinh, John D. Crispino, Trang Hoang, Mahmoud A. Bassal, Daniel G. Tenen
Summary: The transcription factor PU.1 recruits c-Jun as a co-activator to promoters without AP-1 binding sites, which is crucial for macrophage differentiation.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biology
Atsushi Hasegawa, Yuki Hayasaka, Masanobu Morita, Yuta Takenaka, Yuna Hosaka, Ikuo Hirano, Masayuki Yamamoto, Ritsuko Shimizu
Summary: DCML deficiency is an important disorder characterized by loss of multiple immune cell types, potentially caused by mutations in the GATA2 transcription factor. A mouse model carrying GATA2 mutant mutations shows a phenotype similar to human DCML deficiency, providing a new pathway for understanding the molecular mechanisms of GATA2-related hematopoietic diseases.
COMMUNICATIONS BIOLOGY
(2022)
Review
Endocrinology & Metabolism
Robert S. Viger, Karine de Mattos, Jacques J. Tremblay
Summary: Understanding how genes are turned on and off is crucial for the development and function of different cell types. Transcription factors, such as the GATA family, play essential roles in controlling gene expression in the male gonads, impacting testis development, fetal testis gene expression, testicular disease, and XY sex differentiation in humans.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Medicine, Research & Experimental
Mabel Minji Jung, Siqi Shen, Giovanni A. Botten, Thomas Olender, Koichi R. Katsumura, Kirby D. Johnson, Alexandra A. Soukup, Peng Liu, Qingzhou Zhang, Zena D. Jensvold, Peter W. Lewis, Robert A. Beagrie, Jason K. K. Low, Lihua Yang, Joel P. Mackay, Lucy A. Godley, Marjorie Brand, Jian Xu, Sunduz Keles, Emery H. Bresnick
Summary: Decoding the impact of many genetic variants is challenging, but research on a patient with leukemia predisposition syndrome and a germline GATA2 variant provides insight into the underlying mechanisms. The variant was found to be severely defective in regulating transcription and remodeling chromatin, leading to the generation of lineage-diverting gene expression and hematopoiesis-disrupting signaling networks. Insufficient GM-CSF signaling and excessive IL-6 signaling were identified as the causes of specific patient phenotypes.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Biochemistry & Molecular Biology
Dongxin Zeng, Junwen Zheng, Shuwen Feng, Panpan Fan, Dongchi Zhao
Summary: This study demonstrates that B19 NS1 protein negatively regulates GATA1 expression and induces the expression of Hes1/Hes5, thereby interfering with the Notch signaling pathway and potentially playing a role in the negative regulation of hematopoietic cell differentiation.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2022)
Article
Plant Sciences
Delight Hwarari, Yasmina Radani, Yuanlin Guan, Jinhui Chen, Yang Liming
Summary: The endangered Liriodendron chinense has socio-economic and ecological benefits. GATA transcription factors play a significant role in acclimatization to abiotic stresses in L. chinense. The study identified 18 GATA genes in L. chinense and explored their functions.
Article
Plant Sciences
Xiuming Zhang, Jiahui Ma, Shijin Yang, Wenkong Yao, Ningbo Zhang, Xinyi Hao, Weirong Xu
Summary: This study identified and analyzed the GATA gene family in grapevine. It revealed the distribution and regulation of VviGATA genes on grapevine chromosomes and their response to environmental stimuli and hormones. Additionally, the study investigated the function of heterologously expressed GATA genes in cold-resistant grapevine.
Article
Multidisciplinary Sciences
Kenji Kamimoto, Blerta Stringa, Christy M. Hoffmann, Kunal Jindal, Lilianna Solnica-Krezel, Samantha A. Morris
Summary: Cell identity is controlled by the regulation of gene expression in complex gene-regulatory networks. A machine-learning-based approach, CellOracle, is used to simulate transcription factor perturbations and predict changes in cell identity based on single-cell multi-omics data. This approach successfully models reported changes in phenotype caused by transcription factor perturbation and identifies previously unreported phenotypes in zebrafish embryogenesis. The results demonstrate that CellOracle can analyze the regulation of cell identity by transcription factors and provide insights into development and differentiation.
Article
Biochemistry & Molecular Biology
Petra Basova, Helena Paszekova, Lubomir Minarik, Martina Dluhosova, Pavel Burda, Tomas Stopka
Summary: PU.1, a key regulator of hematopoiesis, is influenced by factors such as miR-155, DNA methylation, MYB, and other epigenetic factors. Combinatorial treatment with inhibitors can lead to overproduction of PU.1, stimulating myeloid transcriptional program and inhibiting cell survival and proliferation, ultimately leading to leukemic differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Clinical Neurology
Patricia Martinez-Botia, Angela Velasco, Valeria Rolle, Luis Jimenez-Trevino, Lorena De la Fuente-Toma, Angel Bernardo, Tamara Arias, Maria del Carmen Munoz-Turrillas, Leticia Garcia-Alvarez, Leticia Gonzalez-Blanco, Julia Rodriguez-Revuelta, Maria Paz Garcia-Portilla, Philippe Courtet, Julio Bobes, Laura Gutierrez, Pilar Alejandra Saiz
EUROPEAN NEUROPSYCHOPHARMACOLOGY
(2020)
Article
Hematology
Patricia Martinez-Botia, Andrea Acebes-Huerta, Jerard Seghatchian, Laura Gutierrez
TRANSFUSION AND APHERESIS SCIENCE
(2020)
Article
Cell Biology
Maaike R. Scheenstra, Patricia Martinez-Botia, Andrea Acebes-Huerta, Rutger W. W. Brouwer, Noemi Caballero-Sanchez, Nynke Gillemans, Pieter De Bleser, Benjamin Nota, Iris M. De Cuyper, Vishal Salunkhe, Andrea M. Woltman, Lianne Laar, Erikjan Rijkers, Jeroen A. A. Demmers, Wilfred F. J. IJcken, Sjaak Philipsen, Timo K. den Berg, Taco W. Kuijpers, Laura Gutierrez
Summary: Dendritic cells (DCs) play a crucial role in immune regulation, with the transcription factor PU.1 being identified as a key player in the biology of DCs. Study findings reveal significant interspecies differences between human and mouse DCs, underscoring the importance of studying human DC biology.
JOURNAL OF LEUKOCYTE BIOLOGY
(2021)
Review
Medicine, General & Internal
Patricia Martinez-Botia, Andrea Acebes-Huerta, Jerard Seghatchian, Laura Gutierrez
MEDICINA-LITHUANIA
(2020)
Article
Medicine, General & Internal
Patricia Martinez-Botia, Angel Bernardo, Andrea Acebes-Huerta, Alberto Caro, Blanca Leoz, Daniel Martinez-Carballeira, Carmen Palomo-Antequera, Inmaculada Soto, Laura Gutierrez
Summary: This study evaluated the impact of clinical management on the survival of COVID-19 patients. The results showed that treating hypertension with ACE2 inhibitors or ARBs is not detrimental, and NSAIDs are safe to use. Administering LMWH as an anticoagulant significantly improves the survival of hospitalized COVID-19 patients.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Maria L. Lozano, Cristina Segu-Verges, Mireia Coma, Maria T. Alvarez-Roman, Jose R. Gonzalez-Porras, Laura Gutierrez, David Valcarcel, Nora Butta
Summary: The study reveals that the immunomodulatory effects of Eltrombopag in patients with ITP may be achieved through direct stimulation of megakaryocytes by MPL or MPL-independent effects on other cell types. Mathematical models and structural analyses further elucidate the potential MPL-dependent pathways in non-megakaryocytic cell subtypes affected by Eltrombopag.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Andrea Acebes-Huerta, Patricia Martinez-Botia, Cristina Martin Martin, Angel Bernardo, Adrian R. Rodriguez, Maria Carmen Vicente-Ayuso, Celina Benavente Cuesta, Laura Gutierrez
Summary: The study presents an immunophenotyping strategy to identify successive MK differentiation stages in human primary sources or in vitro cultures, with a panel integrating specific and non-specific surface markers. This approach facilitates a better understanding of megakaryopoiesis complexity.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Multidisciplinary Sciences
Roel M. van Harten, Johanna L. M. Tjeerdsma-van Bokhoven, Astrid de Greeff, Melanie D. Balhuizen, Albert van Dijk, Edwin J. A. Veldhuizen, Henk P. Haagsman, Maaike R. Scheenstra
Summary: The study evaluates the prophylactic capacity of D-CATH-2 and its derivatives in mammalian cells, and finds that they possess strong antibacterial capacity and immunomodulatory function. Administering D-C(1-21) provides partial protection against S. suis infection and reduces disease severity and mortality.
JOURNAL OF ADVANCED RESEARCH
(2022)
Article
Hematology
Maria Villa-Fajardo, Maria Cecilia Yanez Palma, Andrea Acebes-Huerta, Patricia Martinez-Botia, Marjolein Meinders, Martijn A. Nolte, Celina Benavente Cuesta, Johannes A. Eble, Juan Gonzalez del Castillo, Francisco Javier Martin-Sanchez, Laura Gutierrez
Summary: Platelets play a crucial role in maintaining hemostasis and immune modulation. In sepsis patients, alterations in platelet number and aggregation response are observed, potentially triggered by plasma components. Clinical trials are underway to explore the possibility of stimulating platelet production as an alternative to platelet transfusions.
TRANSFUSION AND APHERESIS SCIENCE
(2022)
Article
Orthopedics
Judit Fernandez-Fuertes, Tamara Arias-Fernandez, Andrea Acebes-Huerta, Marlene Alvarez-Rico, Laura Gutierrez
Summary: This study evaluated the clinical efficacy of a standardized PRP product for the treatment of KOA and found that it significantly improved WOMAC scores and achieved basic satisfaction without adverse events. There was no correlation observed between PRP quality parameters and clinical results.
ORTHOPAEDIC JOURNAL OF SPORTS MEDICINE
(2022)
Article
Hematology
Patricia Martinez-Botia, Marjolein Meinders, Iris M. De Cuyper, Johannes A. Eble, John W. Semple, Laura Gutierrez
Summary: In this study, the researchers investigated the dynamic changes in platelet proteome in ITP mouse models, aiming to simulate the acute and chronic stages of ITP in humans. They found specific protein dynamics associated with alterations in platelet aggregation responses. The study suggests that the platelet proteome could provide diagnostic and prognostic insights into ITP.
Article
Medicine, General & Internal
Angel Bernardo, Alberto Caro, Daniel Martinez-Carballeira, Jose Ramon Corte, Sonia Vazquez, Carmen Palomo-Antequera, Alfredo Andreu, Alvaro Fernandez-Pardo, Julia Oto, Laura Gutierrez, Inmaculada Soto, Pilar Medina
Summary: Hemophilia A is a rare bleeding disorder treated with FVIII replacement therapy. Laboratory monitoring of FVIII activity is difficult, while thrombin generation test parameters may accurately reflect the hemostatic status of patients. Combining thrombin generation test parameters with severity of symptoms and F8 mutation type can improve prognostic capacity.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Cell Biology
Patricia Martinez-Botia, Patricia Villar, Graciela Carbajo-Arguelles, Zacaria Jaiteh, Andrea Acebes-Huerta, Laura Gutierrez
Summary: The field of proteomics in platelet biology is rapidly developing and can help identify health-disease markers and novel targets. The comparison of mouse and human platelet proteomes suggests a high conservation, supporting the use of proteomics in both species. Platelet proteomics has clinical relevance and can aid diagnosis, prognosis, and therapy improvement.
Article
Genetics & Heredity
Petros Papadopoulos, Athanassia Kafasi, Iris M. De Cuyper, Vilma Barroca, Daniel Lewandowski, Zahra Kadri, Martijn Veldthuis, Jeffrey Berghuis, Nynke Gillemans, Celina Maria Benavente Cuesta, Frank G. Grosveld, Rob van Zwieten, Sjaak Philipsen, Muriel Vernet, Laura Gutierrez, George P. Patrinos
Article
Hematology
Fanbi Meng, Shuang Chen, Chunliang Liu, Muhammad Shoaib Khan, Yan Yan, Jun Wan, Yue Xia, Chenglin Sun, Mengnan Yang, Renping Hu, Kesheng Dai
Summary: PKC plays a critical role in regulating megakaryocyte development and platelet production in thrombocytopenia. The inhibition of PKC activation delays and reduces radiation-induced megakaryocyte apoptosis, leading to improved platelet count recovery. This study provides a potential therapeutic target for treating thrombocytopenia.
BLOOD CELLS MOLECULES AND DISEASES
(2024)
Article
Hematology
Xia Chen, Fang Liu, Yuanyuan Ren, Luyang Zhang, Yang Wan, Wenyu Yang, Xiaojuan Chen, Li Zhang, Yao Zou, Yumei Chen, Xiaofan Zhu, Ye Guo
Summary: Unrelated umbilical cord blood transplantation (UCBT) is an effective alternative treatment for pediatric patients with bone marrow failure. The use of the TBI/BU + FLU + CY conditioning regimen ensures a high engraftment rate for UCBT and overcomes the challenge of graft failure. Secondary salvage use of cord blood transplantation may still be useful for patients who have failed after other transplantation.
BLOOD CELLS MOLECULES AND DISEASES
(2024)
Article
Hematology
Chithra D. Palani, Xingguo Zhu, Manickam Alagar, Otis C. Attucks, Betty S. Pace
Summary: This study evaluated the ability of a novel Bach1 inhibitor, HPP-D, to induce HbF in KU812 cells and primary sickle erythroid progenitors. The results showed that HPP-D increased HbF levels and decreased Bach1 protein levels in both cell types. Furthermore, it enhanced NRF2 binding to the gamma-globin promoter antioxidant response elements, leading to an open chromatin configuration and increased gamma-globin transcription.
BLOOD CELLS MOLECULES AND DISEASES
(2024)
Letter
Hematology
Marcos Borato Viana, Erica Louback Oliveira, Andre Rolim Belisario
BLOOD CELLS MOLECULES AND DISEASES
(2024)
Article
Hematology
Ke Zhang, Longying Ye, Yanhui Jin, Yuan Chen, Shuting Jiang, Haixiao Xie, Lihong Yang, Mingshan Wang
Summary: This study investigated two pedigrees with FV deficiency and identified three mutations that may have deleterious effects on the function and structure of FV.
BLOOD CELLS MOLECULES AND DISEASES
(2024)