Article
Microbiology
Hidehiro Ito, Kosei Yoshimori, Masahito Ishikawa, Katsutoshi Hori, Toshiaki Kamachi
Summary: In this study, a methanol production system was established using methanotroph mutants to control the expression of methanol dehydrogenase (MDH), allowing for methanol accumulation. By modulating the metal ion composition, the conditions for cell growth inhibition and methanol accumulation were repeatedly alternated, showcasing the potential for efficient conversion of methane to methanol.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Environmental Sciences
Dandan Huang, Wenjun Xu, Qian Wang, Qiyong Xu
Summary: The impact of H2S on the methane oxidation capacity and microbial communities in landfill cover soil (LCS) is influenced by H2S concentration and gas conditions. Addition of biochar (BC) can enhance methane oxidation capacity and exhibit excellent H2S adsorption ability.
Article
Green & Sustainable Science & Technology
Saif Khan, Gourav Jain, Alka Srivastava, Praveen C. Verma, Veena Pande, Rama S. Dubey, Mahvish Khan, Shafiul Haque, Saheem Ahmad
Summary: The need for alternative and environment-friendly fuels has led to the exploration of methanol as a renewable fuel. Methanol can be synthesized from waste biomass using different routes, such as through the involvement of methanotrophs or the enzyme PME. This review discusses the methodologies for utilizing biogas from waste biomass as a feedstock for methanol production and identifies areas for future research to overcome limitations.
SUSTAINABLE MATERIALS AND TECHNOLOGIES
(2023)
Article
Microbiology
Carlo R. Carere, Kiel Hards, Kathryn Wigley, Luke Carman, Karen M. Houghton, Gregory M. Cook, Matthew B. Stott
Summary: Members of the genus Methylacidiphilum, a type of metabolically flexible thermoacidophilic methanotrophs from the phylum Verrucomicrobia, have been shown to utilize formic acid as a substrate for growth when able to maintain pH homeostasis. This demonstrates their ability to grow optimally with a circumneutral intracellular pH across a wide range of extracellular pH values. The findings expand the metabolic flexibility of verrucomicrobial methanotrophs to include organic acids and provide insight into survival strategies during methane starvation.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Rob A. Schmitz, Sepehr S. Mohammadi, Timo van Erven, Tom Berben, Mike S. M. Jetten, Arjan Pol, Huub J. M. Op den Camp
Summary: This study found that the degradation of methanethiol is a widespread detoxification mechanism in verrucomicrobial methanotrophs, which can consume methanethiol and produce H2S. The presence of genes encoding a putative methanethiol oxidase in various methanotrophs suggests that methanethiol degradation is a common process.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Changqing Liu, Federica Angius, Arjan Pol, Rob A. Mesman, Wouter Versantvoort, Huub J. M. Op den Camp
Summary: Bacterial lipoproteins, characterized by a lipid-modified cysteine residue, have important roles in various physiological processes. The highly expressed lipoprotein WP_009060351 was identified in the genome of the methanotrophic bacterium Methylacidiphilum fumariolicum SolV through transcriptome analysis. Heterologous expression of WP_009060351 revealed its dimeric and tetrameric forms, and immunoblotting confirmed its presence in the membrane protein and peptidoglycan fractions. These results suggest the involvement of WP_009060351 in the linkage between the outer membrane and peptidoglycan.
ARCHIVES OF MICROBIOLOGY
(2023)
Article
Microbiology
Changqing Liu, Rob Mesman, Arjan Pol, Federica Angius, Huub J. M. Op den Camp
Summary: In this study, a major outer membrane protein (WP_009059494) from Methylacidiphilum fumariolicum SolV was identified and characterized. The protein was found to be mainly present in the outer membrane and resistant to harsh conditions, suggesting its role in resisting extreme environmental conditions. Additionally, the absence of specific proteins in the canonical BAM complex was observed in the Methylacidiphilum and Methylacidimicrobium species.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Subhasree Ray, Jun-O Jin, Inho Choi, Myunghee Kim
Summary: Growing concerns over limited fossil fuel use and their negative impacts on the environment have led to exploration of alternative routes. One such eco-friendly alternative is polyhydroxyalkanoates, which are biodegradable and biocompatible. Researchers have focused on utilizing waste gases as substrates for polyhydroxyalkanoates production, with microorganisms like Methylobacterium organophilum and Cupriavidus necator showing promising results. This article discusses the potential of waste gas valorization for sustainable production of polyhydroxyalkanoates and highlights key challenges and possible technological solutions.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Energy & Fuels
Michael Bampaou, Kyriakos Panopoulos, Panos Seferlis, Amaia Sasiain, Stephane Haag, Philipp Wolf-Zoellner, Markus Lehner, Leokadia Rog, Przemyslaw Rompalski, Sebastian Kolb, Nina Kieberger, Stefano Dettori, Ismael Matino, Valentina Colla
Summary: This study investigates the cost-efficient integration of renewable hydrogen into steelworks for the production of methane and methanol, and explores their economic performance under different parameters. The study identifies renewable hydrogen as the most expensive component in this scheme and emphasizes the need for progress in the hydrogen economy.
Article
Microbiology
Ramita Khanongnuch, Rahul Mangayil, Ville Santala, Anne Grethe Hestnes, Mette Marianne Svenning, Antti J. Rissanen
Summary: This study explores the concept of utilizing methane-oxidizing bacteria to produce organic acids from methane and using them as feedstocks for heterotrophic bacteria. Experimental results show that the organic acid-rich spent media of methane-oxidizing bacteria can be used for 1-alkene production. However, further research and optimization are required.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Physical
Jose L. C. Fajin, M. Natalia D. S. Cordeiro
Summary: Ni-Cu catalysts are low cost, highly selective for CO2 and H-2 in methanol steam reforming, blocking the production of methane, CO, and coke. The mechanism of methanol steam reforming on Ni-Cu surfaces involves methanol decomposition followed by the water-gas shift reaction, with a minority route for direct CO2 formation. The Ni-Cu alloy suppresses methane and coke formation and has a high desorption barrier for CO species, avoiding its production.
Article
Energy & Fuels
Ahmad Razi Othman, Yap Jun Sheng, Noorhisham Tan Kofli, Siti Kartom Kamaruddin
Summary: The research focused on the growth characteristics and optimal conditions of the methanotroph Methylosinus trichosporium for methanol production, indicating that the bacterium can maximize methanol production under specific methane-air ratio, pH, and phosphate ion concentration.
GREENHOUSE GASES-SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Ye Rim Park, Dong Ho Kim, Kyu Hwan Choi, Yong Woo Kim, Eun Yeol Lee, Bum Jun Park
Summary: An efficient phase-transfer catalysis system for converting methane to methanol using methanotrophs is reported in this study. The system increases methanol productivity and lowers production costs. The use of a cellulose-membrane reactor system allows for multiple biocatalytic reactions without significant decrease in productivity.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2021)
Article
Environmental Sciences
Xiaoqi Zhou, Simeon J. Smaill, Xinyun Gu, Peter W. Clinton
Summary: The study found that under drought conditions, reducing in planta C2H4 production can increase the oxidation of methane in the soil, providing a reliable means for increasing soil methane oxidation in the context of global warming.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biotechnology & Applied Microbiology
Ingeborg Bussmann, Fabian Horn, Michael Hoppert, Karl-Walter Klings, Anke Saborowski, Julia Warnstedt, Susanne Liebner
Summary: Three strains of methanotrophic bacteria were isolated from the River Elbe in Germany, including two novel species within the genus Methylomonas and one member of the species Methylovulum psychrotolerans. These bacteria exhibit differences in carbon source utilization and growth conditions, highlighting the importance of identifying and classifying new species in this study.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2021)
Article
Microbiology
Jodie A. Schildkraut, Jordy P. M. Coolen, Sophie Burbaud, Jasper J. N. Sangen, Michael P. Kwint, R. Andres Floto, Huub J. M. op den Camp, Lindsey H. M. Te Brake, Heiman F. L. Wertheim, Kornelia Neveling, Wouter Hoefsloot, Jakko van Ingen
Summary: This study provides the first description of the major transcriptional mechanisms of tolerance to recommended antibiotics in Mycobacterium abscessus. The bacterium shows both drug-specific and common transcriptomic responses to antibiotic exposure. The findings have significant implications for understanding antibiotic resistance mechanisms and developing targeted therapeutic regimens.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Koen A. J. Pelsma, Michiel H. in 't Zandt, Huub J. M. Op den Camp, Mike S. M. Jetten, Joshua F. Dean, Cornelia U. Welte
Summary: The study investigated the methane-cycling community of the Amsterdam canal system in the Netherlands through microcosm incubations and molecular analyses, revealing the presence of microbes with methane metabolism potential in sediment and canal wall biofilms. These findings highlight the importance of previously unidentified and specialised environmental niches in urban aquatic ecosystems at the nexus of the natural and human-impacted carbon cycle.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Paula Dalcin Martins, Maider J. Echeveste Medrano, Arslan Arshad, Julia M. Kurth, Heleen T. Ouboter, Huub J. M. op den Camp, Mike S. M. Jetten, Cornelia U. Welte
Summary: Microbial communities play a crucial role in carbon, sulfur, and nitrogen cycling in coastal ecosystems. However, little is known about how these communities respond to changes in substrate availability and exposure to toxic compounds. In this study, metagenomics and metatranscriptomics were used to investigate the structure and transcriptional responses of microbial communities in a controlled bioreactor system mimicking coastal sediment conditions. The results showed that some microbial species were more resilient to experimental conditions than others, and certain community functions, such as methane and sulfur oxidation, remained stable despite shifts in community structure. Further studies are needed to understand how coastal ecosystems respond to future disturbances.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Environmental Sciences
Min Cai, Shuai Li, Fei Ye, Yiguo Hong, Mingquan Lu, Huub J. M. Op den Camp, Yu Wang
Summary: Small waters, such as ponds, are important freshwater environments that have been increasingly recognized for their role in ecosystem service delivery. In agricultural watersheds, artificial ponds play a vital role in reducing nitrogen pollution. However, artificial ponds have been poorly investigated compared to other water environments. The importance of microbial activities in these ponds and their role in nitrogen removal are not well understood. To study the microbial nitrogen removal in agricultural watersheds, sediment samples from 21 ponds and 11 soil samples were collected in China. The results revealed that pond sediments had significantly higher nitrogen and organic matter content compared to surrounding soils. The microbial nitrogen removal rates were also much higher in ponds than in dryland soils. Denitrification was found to be the dominant process in nitrogen removal in ponds, while anaerobic ammonium oxidation contributed only minimally. These findings highlight the ecosystem function of ponds in agricultural watersheds and provide new insights into pollution control and global nitrogen cycling.
Article
Microbiology
Rob A. Schmitz, Sepehr S. Mohammadi, Timo van Erven, Tom Berben, Mike S. M. Jetten, Arjan Pol, Huub J. M. Op den Camp
Summary: This study found that the degradation of methanethiol is a widespread detoxification mechanism in verrucomicrobial methanotrophs, which can consume methanethiol and produce H2S. The presence of genes encoding a putative methanethiol oxidase in various methanotrophs suggests that methanethiol degradation is a common process.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Fei Ye, Yiguo Hong, Jiapeng Wu, Xuemei Yi, Huub J. M. Op den Camp, Selina Sterup Moore, Teofilo Vamerali, Yu Wang
Summary: The succession of microbial and plant communities is crucial for the development and stability of soil ecological functions. This study examined the effects of environmental disturbance and plant community traits on the succession of the soil microbial community in a newly established habitat. The findings highlight the importance of understanding the role of environmental disturbance and plants in shaping soil microbial community assemblage.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Helena Singer, Robin Steudtner, Andreas S. Klein, Carolin Rulofs, Cathleen Zeymer, Bjoern Drobot, Arjan Pol, N. Cecilia Martinez-Gomez, Huub J. M. Op den Camp, Lena J. Daumann
Summary: This study investigates the ability of actinides, the radioactive 5f elements, to replace the essential 4f elements in lanthanide-dependent bacterial metabolism. The results show that americium and curium can support growth in the absence of lanthanides, and that methylotrophic bacteria prefer actinides over late lanthanides when presented with a mixture of equal amounts of lanthanides together with americium and curium. This study establishes that methylotrophic bacteria can utilize actinides instead of lanthanides to sustain their one-carbon metabolism if they possess the correct size and a +III oxidation state.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biotechnology & Applied Microbiology
Heleen T. Ouboter, Arslan Arshad, Stefanie Berger, Jesus Gerardo Saucedo Sanchez, Huub J. M. op den Camp, Mike S. M. Jetten, Cornelia U. Welte, Julia M. Kurth
Summary: This study describes the biochemical properties of ACS and ACD enzymes from ANME-2 archaea, adding to our knowledge of these enzymes. It also validates the in situ activity of ACS in ANME-2d, demonstrating the conversion of acetate into methane.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Microbiology
Changqing Liu, Federica Angius, Arjan Pol, Rob A. Mesman, Wouter Versantvoort, Huub J. M. Op den Camp
Summary: Bacterial lipoproteins, characterized by a lipid-modified cysteine residue, have important roles in various physiological processes. The highly expressed lipoprotein WP_009060351 was identified in the genome of the methanotrophic bacterium Methylacidiphilum fumariolicum SolV through transcriptome analysis. Heterologous expression of WP_009060351 revealed its dimeric and tetrameric forms, and immunoblotting confirmed its presence in the membrane protein and peptidoglycan fractions. These results suggest the involvement of WP_009060351 in the linkage between the outer membrane and peptidoglycan.
ARCHIVES OF MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Rob A. Schmitz, Stijn H. Peeters, Sepehr S. Mohammadi, Tom Berben, Timo van Erven, Carmen A. Iosif, Theo van Alen, Wouter Versantvoort, Mike S. M. Jetten, Huub J. M. op den Camp, Arjan Pol
Summary: Aerobic methanotrophic bacteria can survive in sulfide-rich environments, despite the inhibitory effects of hydrogen sulfide (H2S) on methane oxidation and aerobic respiration. This is due to the upregulation of a sulfide-insensitive terminal oxidase, allowing the microorganism to oxidize both methane and H2S simultaneously.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Carmen Hogendoorn, Arjan Pol, Rob de Graaf, Paul B. White, Rob Mesman, Peter M. van Galen, Theo A. van Alen, Geert Cremers, Robert S. Jansen, Mike S. M. Jetten, Huub J. M. Op den Camp
Summary: The production of organic molecules can be achieved by synthesizing them from CO2 and a cheap energy source, instead of relying on fossil fuels. Volcanic and geothermal areas, which have abundant CO2 and reduced inorganic gases, provide habitats where novel chemolithoautotrophic microorganisms for organic compound synthesis can be discovered. A thermoacidophilic, autotrophic H-2-oxidizing microorganism called Candidatus Hydrogenisulfobacillus filiaventi R50 was found to fix CO2 and produce more than 0.54 mol of organic carbon per mole of fixed CO2. This discovery opens up possibilities for the sustainable production of important biomolecules using chemolithoautotrophic microorganisms.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Changqing Liu, Rob Mesman, Arjan Pol, Federica Angius, Huub J. M. Op den Camp
Summary: In this study, a major outer membrane protein (WP_009059494) from Methylacidiphilum fumariolicum SolV was identified and characterized. The protein was found to be mainly present in the outer membrane and resistant to harsh conditions, suggesting its role in resisting extreme environmental conditions. Additionally, the absence of specific proteins in the canonical BAM complex was observed in the Methylacidiphilum and Methylacidimicrobium species.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Helena Singer, Robin Steudtner, Ignacio Sottorff, Bjoern Drobot, Arjan Pol, Huub J. M. Op den Camp, Lena J. Daumann
Summary: We introduce an extremophilic bacterium called Methylacidiphilum fumariolicum SolV as a potential platform for recovering rare earth elements (REE). Strain SolV demonstrates the ability to selectively extract light REE from industrial waste, natural REE-containing sources, and post-mining waters. Scaling up, modifying media composition, and cycling accumulation were successfully implemented, highlighting the potential for bio-recovery of REE.
CHEMICAL COMMUNICATIONS
(2023)
Article
Engineering, Environmental
Ana B. Rios-Miguel, Garrett J. Smith, Geert Cremers, Theo van Alen, Mike S. M. Jetten, Huub J. M. Op den Camp, Cornelia U. Welte
Summary: Pharmaceutical micropollutants pose environmental risks by entering water bodies worldwide. Paracetamol plays a unique role in microbial degradation, but the genes and enzymes involved are still unknown. Metagenomic analysis of a bioreactor revealed a diverse microbial community, including two Pseudomonas species, suggesting a complex pathway for paracetamol degradation.
Article
Chemistry, Physical
Nader Al Danaf, Jerome Kretzschmar, Berenice Jahn, Helena Singer, Arjan Pol, Huub J. M. Op den Camp, Robin Steudtner, Don C. Lamb, Bjoern Drobot, Lena J. Daumann
Summary: In this study, the effects of pH and temperature on the distribution of different PQQ species were investigated using NMR, UV-Vis spectroscopy, and TRLFS. The binding of Eu(iii) to the active sites of MDH enzymes was monitored using two techniques, TRLFS and antenna effect.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)