Article
Chemistry, Analytical
Adam Shebindu, Himali Somaweera, Zachary Estlack, Jungtae Kim, Jungkyu Kim
Summary: This study introduces a programmable microfluidic platform (PMP) combined with isotachophoresis (ITP) for fully automated optimization of multiple analytes. By utilizing lifting-gate microvalve technology, a two-dimensional microvalve array was designed and fabricated to seamlessly integrate with an ITP chip for automated buffer selection and repetitive ITP procedures. The optimal buffer combination of 40mM borate and 100mM Tris-HCl successfully preconcentrated all analytes during a single ITP run.
Article
Food Science & Technology
Jun Feng, Jinxia Li, Wenyi Huang, Hao Cheng, Zhiyong Zhang, Lijun Li
Summary: Transient isotachophoresis (tITP) is a universal online sample preconcentration technology that improves the sensitivity of capillary electrophoresis (CE). A new approach under counter-electroosmotic flow for the determination of five trace food additives has been developed, demonstrating significant improvements in sensitivity and detection capabilities. The method has been shown to be simple, rapid, and sensitive, with successful applications in separating and detecting trace food additives in beverage samples.
FOOD ANALYTICAL METHODS
(2021)
Article
Biochemical Research Methods
Frantisek Kvasnicka, Ales Rajchl
Summary: The research presents an electrophoretic method for the determination of free taurine in selected food and feed, with optimized conditions and performance characteristics evaluated. The method shows high sensitivity and selectivity, short analysis time, and low costs, making it suitable for routine analysis.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Annestasia Simbut John, Mohammad Mizwaruddin Sidek, Lee Yien Thang, Sabita Sami, Hui Yin Tey, Hong Heng See
Summary: This review provides a comprehensive overview of various online preconcentration techniques in nonaqueous capillary and microchip electrophoresis, including sample stacking, isotachophoresis, and micellar-based stacking. Attention is also given to multi-stacking strategies used for nonaqueous electrophoresis.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Review
Chemistry, Analytical
Zdena Mala, Petr Gebauer
Summary: Analytical isotachophoresis is a well-established electrophoretic separation method that allows for the analysis of mixtures and trace analysis. It has both concentrating and separating capabilities and can be used with complex-matrix samples. Recent applications have shown interest in its concentrating properties and its incorporation into multistage analysis and micropreparative procedures. This review highlights the continual development and contributions of isotachophoresis in contemporary analytical chemistry.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Biophysics
Waleed Alahmad, Ali Sahragard, Pakorn Varanusupakul
Summary: Microfluidic paper-based analytical devices (μPADs) have gained attention for their advantages in clinical diagnostics, environmental monitoring, and food safety assurance. However, they lack high sensitivity for detecting trace analytes compared to commercial instruments like mass spectrometry. To enhance sensitivity, a preconcentration step is required. This review focuses on techniques to separate and preconcentrate analytes on μPADs, along with challenges and future perspectives.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Applied
Jude Caleb, Usama Alshana, Nusret Ertas
Summary: A novel method for the determination of iodate ions using smartphone digital image colorimetry combined with solidification of floating organic drop-dispersive liquid-liquid microextraction was proposed in this study. The method showed high sensitivity, good linearity, and was efficiently applied in the analysis of iodate ions in table salt samples.
Article
Biochemistry & Molecular Biology
Izabella Koska, Krystian Purgat, Rafal Glowacki, Pawel Kubalczyk
Summary: The developed method combines capillary electrophoresis and liquid-liquid extraction for the determination of ciprofloxacin and ofloxacin in meat tissues. The results show that the method is accurate, with good linearity and recovery rates.
Article
Biochemical Research Methods
Gyula Vigh, Bohuslav Gas
Summary: The carrier ampholytes-based isoelectric focusing has evolved from a closed system with steady pH gradient to an open system with transient pH gradient. The open system follows a two-stage model, where a rapid IEF process forms the pH gradient in the first stage, and isotachophoretic processes degrade the pH gradient in the second stage. The outcome of the process is determined by ionic mobilities, pK(a) values, and loaded amounts of all components, and a steady state cannot be achieved in terms of spatial coordinate.
Article
Chemistry, Applied
Marcin Musielak, Maciej Serda, Rafal Sitko
Summary: The paper presents novel analytical methods for sensitive mercury determination in various samples using graphene oxide/thiosemicarbazide in dispersive micro-solid phase extraction (DMSPE) and detection by energy-dispersive (EDXRF) and total-reflection X-ray fluorescence spectrometry (TXRF). The methods demonstrate high enrichment factors and low detection limits, with DMSPE/TXRF achieving LODs of 2.1 pg mL(-1) for liquids and 1.8 ng g(-1) for solid samples.
Article
Chemistry, Analytical
Antonio Cobelo-Garcia, Mirsya E. Mulyani, Jorg Schafer
Summary: The determination of palladium in environmental samples by ICP-MS is challenging due to extensive interferences, but an interference-free methodology for the determination of Pd in natural waters has been successfully achieved by online matrix separation and preconcentration. This method allows for the detection of dissolved Pd in natural samples at low, ambient concentrations, providing valuable data for understanding the behavior of Pd in natural waters.
Article
Chemistry, Multidisciplinary
Salman S. Alharthi, Hamed. M. Al-Saidi
Summary: A simple semi-automated dispersive liquid-liquid microextraction system was developed for the preconcentration and determination of nickel (II) ion in food samples. The method showed good efficiency in sensitivity and selectivity, with a linear calibration plot and low detection limit. Additionally, the method was free of most potential interferences and was validated using ICP-MS with certified reference samples.
ARABIAN JOURNAL OF CHEMISTRY
(2022)
Editorial Material
Plant Sciences
Aiswarya Girija, Habte Jifar, Chris Jones, Rattan Yadav, John Doonan, Luis A. J. Mur
Summary: This article introduces tef, a highly nutritious gluten-free Ethiopian cereal, and highlights the factors that affect its productivity. With the recent availability of tef genome sequences, there is a need to harness its benefits to improve food security.
TRENDS IN PLANT SCIENCE
(2022)
Article
Chemistry, Analytical
Kamolrat Phomai, Sam-ang Supharoek, Jitlada Vichapong, Kate Grudpan, Kraingkrai Ponhong
Summary: This study presents a new method using iron-tannic nanoparticles as an adsorbent, combined with cloud point extraction, for the enrichment of four tetracyclines prior to HPLC determination. The method demonstrates high extraction and enrichment efficiency, as well as rapidity and environmental friendliness.
Article
Chemistry, Applied
Mustafa Soylak, Furkan Uzcan, Oguzhan Goktas, Zinar Pinar Gumus
Summary: A magnetic nanocomposite was synthesized and applied for the separation and detection of cadmium in water, food, and environmental samples. The method showed improved extraction efficiency after optimization and was suitable for determining cadmium content within different ranges.
Article
Biochemical Research Methods
Tobias Roesch, Gerhard Weber, Tobias Bader, Anna-Jorina Wicht, Carolin Huhn
Summary: Efficient sample enrichment and cleanup techniques are crucial for the analysis of low concentrations of micropollutants in environmental water samples. In this study, field-step electrophoresis (FSE) was developed as a cleanup technique for ionizable micropollutants. The FSE/RPLC-MS method exhibited high precision and selectivity for anionic compounds.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Hannes Georg Graf, Sonja Maria Biebl, Linda Muller, Christina Breitenstein, Carolin Huhn
Summary: Thermodynamic acidity constants and limiting ionic mobilities of polyprotic non-chromophore analytes were determined using capillary electrophoresis with capacitively coupled contactless conductivity detection. Ionic strength effects on electrophoretic mobilities were corrected by modeling, eliminating the need for identical ionic strength buffers. This method allowed for high resolution determination of mobility data in a wide pH range, and was successfully used to determine acidity constants and mobilities for various acidic herbicides.
JOURNAL OF SEPARATION SCIENCE
(2022)
Editorial Material
Biotechnology & Applied Microbiology
Shun Li, Andreas Kappler, Stefan B. Haderlein, Yong-Guan Zhu
Summary: Geobatteries are redox-active substances that can reversibly take up, store, and release electrons, and have the potential to improve the performance of engineered systems.
TRENDS IN BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Anna-Jorina Wicht, Katharina Heye, Anja Schmidt, Joerg Oehlmann, Carolin Huhn
Summary: Insects with aquatic life stages can transfer sediment and water pollutants to terrestrial ecosystems. This study fills the knowledge gap on the transfer of the micropollutant carbamazepine from larvae to adult midges, showing that the uptake is concentration-dependent and reduces the emergence rate. However, the body burden remains constant in adult midges. The daily exposure of insectivorous tree swallows to carbamazepine as terrestrial predators was estimated using the energy demand of the predator and the energy content of the prey.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Engineering, Environmental
Philipp R. Martin, Daniel Buchner, Maik A. Jochmann, Martin Elsner, Stefan B. Haderlein
Summary: The oxidation of aminopolyphosphonate chelating agents such as aminotrismethylene phosphonate (ATMP) by molecular oxygen catalyzed by Mn(II) is an important process in environmental fate. The potential roles of Mn(III)ATMP species in the transformation mechanisms are not fully understood. This study revealed two parallel ATMP transformation pathways, one involving the oxidation of ATMP in Mn(III)ATMP complexes and the other involving the oxidation of free ATMP by Mn(III)ATMP species. These findings highlight the significance of Mn-ATMP species in the overall ATMP oxidation and suggest that Mn(III)ATMP could also act as a potent oxidant for other reducible solutes in aqueous environments.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Agronomy
Benedikt Wimmer, Harald Neidhardt, Marc Schwientek, Stefan B. Haderlein, Carolin Huhn
Summary: A new extraction method combined with capillary electrophoresis-mass spectrometry was developed for the analysis of glyphosate and aminomethylphosphonic acid in soil and sediment samples. The method showed high recovery, low detection limit, and robustness.
PEST MANAGEMENT SCIENCE
(2022)
Article
Environmental Sciences
Johannes Wirsching, Benedikt Wimmer, Franziska Ditterich, Johanna Schloegl, Fabrice Martin-Laurent, Carolin Huhn, Stefan Haderlein, Ellen Kandeler, Christian Poll
Summary: Glyphosate and its metabolite AMPA are frequently detected in European agricultural topsoils. This study found that shrinkage cracks occurring after dry periods could facilitate GLP transport to greater depths where subsoil conditions slow further microbial degradation. The degradation pathway to AMPA dominated, and degradation mainly occurred in Gram+- bacteria.
ENVIRONMENTAL POLLUTION
(2022)
Article
Engineering, Environmental
Adrian Mellage, Manuel Doerrich, Stefan B. Haderlein
Summary: Glyphosate sorption onto natural calcareous aquifer sediment was found to exhibit strong nonlinear reversible retention, proportional to the solid-bound concentrations. The contribution of sorption to pore fluid conduction masked variations in imaginary conductivity. The findings link SIP signal variations to sorption dynamics and provide a framework for improved monitoring of charged organic contaminants in natural sediments.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Biochemical Research Methods
Daniel Buchner, Philipp R. Martin, Johannes Scheckenbach, Steffen Kuemmel, Faina Gelman, Stefan B. Haderlein
Summary: The development of reliable GC/qMS methods for delta Cl-37 compound-specific stable isotope analysis (CSIA) allows for dual carbon-chlorine isotope analysis of chlorinated ethenes. A new Cl-37-enriched PCE standard was prepared and evaluated to extend the calibration range, preventing potential bias introduced by extrapolation. The obtained PCE (PCEenriched) showed a purity of 98.8% and a delta Cl-37(SMOC) value of +10.8 ± 0.5 parts per thousand.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2022)
Article
Environmental Sciences
Benedikt Wimmer, Adrian Langarica-Fuentes, Erik Schwarz, Sara Kleindienst, Carolin Huhn, Holger Pagel
Summary: In this study, we investigated the sorption-limited biodegradation of glyphosate and its main transformation product AMPA in soil using microcosm experiments. Our findings suggest that the degradation of both compounds is influenced by sorption processes, with a biphasic dissipation pattern. The model simulations also indicate that while glyphosate is rapidly transformed in solution, AMPA persists in soil due to its higher dissolved and bioavailable fraction.
JOURNAL OF ENVIRONMENTAL QUALITY
(2023)
Article
Environmental Sciences
Daniel Buchner, Philipp R. Martin, Johannes Scheckenbach, Sebastian Behrens, Stefan B. Haderlein
Summary: The variability of carbon and chlorine isotope enrichment factors for TCE respiration limits the applicability of CSIA for quantitative assessment. The effects of environmentally relevant variables on microbial TCE isotope fractionation were investigated, and the results showed that growth conditions have little effect on isotope fractionation. This suggests that environmental factors likely do not contribute to the reported variability of carbon and chlorine isotope enrichment factors for TCE biotransformation.
Article
Chemistry, Analytical
Johanna Schloegl, Benedikt Wimmer, Lena Cramaro, Johannes Wirsching, Christian Poll, Holger Pagel, Ellen Kandeler, Carolin Huhn, Christian Griebler, Christine Stumpp, Stefan B. Haderlein
Summary: This study presents field data on the effects of heavy rainfall after drought on the mobility of glyphosate and redox conditions in clayey floodplain soil. The findings show that shrinkage cracks in dry soils can act as pathways for the transport of oxygen, water, and glyphosate. Rainfall can translocate water and glyphosate to the subsoil within a few hours. Soil wetting leads to changes in redox conditions, with deeper depths becoming permanently reducing while shallower depths fluctuate between reducing and oxidizing conditions.
ENVIRONMENTAL SCIENCE-PROCESSES & IMPACTS
(2022)