Article
Microscopy
Julia M. T. Auer, Laura C. Murphy, Dong Xiao, David U. Li, Ann P. Wheeler
Summary: Molecular interactions are crucial in cellular processes, especially in gene regulation. Non-fitting lifetime-based FRET variants, such as AAT-FRET, offer a sensitive and simple method to detect and measure protein-protein interactions in live 3D samples.
JOURNAL OF MICROSCOPY
(2023)
Review
Cell Biology
Selina K. Jorch, Carsten Deppermann
Summary: Leukocytes, particularly neutrophils and macrophages from the innate immune system, are crucial for immune responses and defense against pathogens. Novel imaging techniques have enabled detailed insights into the nature and function of these cells, but current limitations still exist.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biophysics
Hyungjin Kim, Daniel Roth, Yasuhiro Isoe, Koichiro Hayashi, Chihiro Mochizuki, Markus Kalkum, Michihiro Nakamura
Summary: In the presence of FBS, cellular uptake of FNP-PEGs was significantly reduced; pre-coating with BSA and Hb enhanced the stealth function of PEGs; BSA and Hb may act as stealth enhancement proteins for FNP-PEGs in vitro.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Medicine, Research & Experimental
Marco Erreni, Francesca D'Autilia, Roberta Avigni, Evangelia Bolli, Sana M. Arnouk, Kiavash Movahedi, Pieterjan Debie, Achille Anselmo, Raffaella Parente, Cecile Vincke, Fijs W. B. van Leeuwen, Paola Allavena, Cecilia Garlanda, Alberto Mantovani, Andrea Doni, Sophie Hernot, Jo A. Van Ginderachter
Summary: Nanobodies (Nbs) have been identified as an elegant alternative to conventional monoclonal antibodies for cancer therapy, but there is a lack of detailed microscopic insight into the in vivo pharmacokinetics of different Nb formats in tumor-bearers, especially for the targeting of pro-tumoral tumor-associated macrophages (TAMs) located in less penetrable tumor regions.
Article
Chemistry, Multidisciplinary
Mengyu Guo, Lina Zhao, Jing Liu, Xiaofeng Wang, Haodong Yao, Xueling Chang, Ying Liu, Jiaming Liu, Min You, Jiayu Ren, Fuhui Wang, Liming Wang, Yaling Wang, Huibiao Liu, Yuliang Li, Yuliang Zhao, Rong Cai, Chunying Chen
Summary: The study shows that carbon-based nanomaterial GDYO nanosheets interact with STAT3 protein, inducing the reeducation of immunosuppressive macrophages and triggering immune responses in the tumor microenvironment, potentially used for cancer immunotherapy.
Article
Chemistry, Multidisciplinary
Wang Sik Lee, Hyunjung Kim, Yugyeong Sim, Taejoon Kang, Jinyoung Jeong
Summary: Nanoplastics, emerging environmental pollutants, pose significant health concerns. Polypropylene, one of the most widely used plastics, can form nanoparticles through weathering, but their biological effects are poorly understood. This study developed a simple method to produce polypropylene nanoplastics and investigated their biodistribution in developing zebrafish embryos.
Review
Pharmacology & Pharmacy
Jeffrey Momoh, Dimitrios Kapsokalyvas, Michael Vogt, Sjoerd Hak, Fabian Kiessling, Marc van Zandvoort, Twan Lammers, Alexandros Marios Sofias
Summary: Intravital microscopy (IVM) has broad applications in various fields, including biology, physiology, immunology, and drug development. This review provides an overview of the modalities, methods, and model organisms used in IVM, as well as its applications in nanomedicine and biological process studies.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Review
Immunology
Anika Gruneboom, Oliver Aust, Zuelal Cibir, Flora Weber, Dirk M. Hermann, Matthias Gunzer
Summary: Innate immune cells play a crucial role in defense against infections and sterile inflammation, with recent advances in microscopy providing insights into their biology. Neutrophils and macrophages have protective roles during tumor growth and ischemia-reperfusion damage, but can also exacerbate diseases. Future directions include enhancing knowledge about innate immune cells and their potential applications in disease diagnostics.
IMMUNOLOGICAL REVIEWS
(2022)
Article
Cell Biology
Laura Dietz, Jennifer Oberlaender, Ana Mateos-Maroto, Jenny Schunke, Michael Fichter, Eva-Maria Kraemer-Albers, Katharina Landfester, Volker Mailaender
Summary: This study analyzed the protein composition of extracellular vesicles (EVs) and the protein corona around EVs in human blood plasma using a proteomic approach. The influence of the protein corona on the uptake of EVs by human monocytes was then compared with the uptake of engineered liposomes. The results showed that the presence of a protein corona increased the uptake of EVs in human monocytes, indicating a clear difference between EVs and liposomes as nanocarriers.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Infectious Diseases
Jinling Fang, Rifeng Chen, Donghua Liu
Summary: This study explores the association between Talaromyces marneffei and CD86 protein, revealing that the fungus has the ability to capture CD86 proteins from macrophages.
INFECTION AND DRUG RESISTANCE
(2022)
Letter
Oncology
Shrikant B. Mali
Summary: Intravital microscopy and optical coherency tomography are powerful imaging tools that have revolutionized cancer diagnosis and therapies. They allow for real-time observation of biological processes in vivo, providing insights into tumor behaviors. Recent developments in techniques for observing deep tissues have improved the detection of subcellular events and made these technologies indispensable in basic sciences and drug development.
Article
Multidisciplinary Sciences
Ayana Sugizaki, Keisuke Sato, Kazuyoshi Chiba, Kenta Saito, Masahiko Kawagishi, Yuri Tomabechi, Shalin B. Mehta, Hirokazu Ishii, Naoki Sakai, Mikako Shirouzu, Tomomi Tani, Sumio Terada
Summary: Biomolecular assemblies play a crucial role in cell physiology, with their function depending on the changes in molecular positions and orientations. Fluorescence polarization microscopy offers a promising solution by reporting the orientation of emission dipoles, providing insights into monitoring molecular orientation in real time within living cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Physical
D. Andersen, H. Chen, S. Pal, L. Cressa, O. De Castro, T. Wirtz, G. Schmitz, S. Eswara
Summary: In this study, high-resolution imaging of hydrogen and deuterium within Mg2Ni/Mg2NiH4 hydrogen storage thin films was achieved using a self-developed secondary ion mass spectrometer (SIMS) system attached to a commercially available dual-beam focused ion beam - scanning electron microscope (FIB-SEM) instrument. A novel approach using laser scanning confocal microscopy (LSCM) was demonstrated to measure the size, shape, and distribution of the hydride phase in partially transformed films. The combination of these techniques provides new insights on hydride nucleation and growth within the Mg2NiHx system. Additionally, the efficacy of tracking deuterium as a hydrogen analog for SIMS imaging in high-vacuum chambers was demonstrated.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Article
Environmental Sciences
Yuming Chen, Jitong Li, Peng Yuan, Zhineng Wu, Zhaoxin Wang, Weidong Wu
Summary: The study reveals that graphene oxide (GO) promotes chromium (Cr) uptake by zebrafish embryos, accelerating the aggregation and settlement of Cr, enhancing the interaction between chorions and contaminants. The presence of GO leads to accumulation of chromium in chorions and intra-chorion embryos, causing oxidative stress and metabolic disturbances.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Multidisciplinary
Krupa Kansara, Abdulkhalik Mansuri, Anjali Rajwar, Payal Vaswani, Ramesh Singh, Ashutosh Kumar, Dhiraj Bhatia
Summary: Three-dimensional DNA nanocages have potential biomedical applications, but their cellular uptake and intracellular fate have not been well explored. This study provides a detailed understanding of the uptake of DNA nanocages in zebrafish embryos and larvae, showing that tetrahedrons exhibit significant internalization without affecting the expression of genes involved in embryo development. These findings contribute valuable insights into the internalization and biocompatible potential of DNA nanocages for biomedical applications.
NANOSCALE ADVANCES
(2023)
Review
Endocrinology & Metabolism
Claus Oxvig, Cheryl A. Conover
Summary: PAPP-A and PAPP-A2 are specific proteolytic enzymes involved in IGF signaling regulation by cleaving IGFBPs and increasing IGF bioactivity. This molecular network plays a significant role in the IGF system, but its precise operation needs further investigation.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Review
Endocrinology & Metabolism
Cheryl A. Conover, Claus Oxvig
Summary: PAPP-A is a placental protein that was initially discovered in pregnant women and later found to be a metalloproteinase expressed by nonplacental cells. It regulates local insulin-like growth factor (IGF) activity by cleaving high-affinity IGF binding proteins (IGFBPs). This proteolytic regulation is important for cell proliferation, differentiation, migration, and survival in normal and cancer cells.
Article
Chemistry, Applied
Hossein Mohammad-Beigi, Wahyu Wijaya, Mikkel Madsen, Yuya Hayashi, Ruifen Li, Tijs Albert Maria Rovers, Tanja Christine Jaeger, Alexander K. Buell, Anni Bygvra Hougaard, Jacob J. K. Kirkensgaard, Peter Westh, Richard Ipsen, Birte Svensson
Summary: In this study, the conformational stability and aggregation propensity of 13-lactoglobulin (13-Lg) and three types of caseins (CNs) were systematically evaluated. The results showed that 13-Lg was affected by heat treatment, while a-CN and 13-CN were influenced by Ca2+. The presence of CNs resulted in smaller mixed aggregates compared to pure 13-Lg aggregates. The study provides insight into how micro-structural assembly of milk proteins can be modulated to enable different functionalities in milk-based products.
FOOD HYDROCOLLOIDS
(2023)
Article
Cardiac & Cardiovascular Systems
Morten Krogh Christiansen, Kasper Kjaer-Sorensen, Natacha C. Clavsen, Sven Dittmann, Maja Fuhlendorff Jensen, Halvor Osterby Guldbrandsen, Lisbeth Norum Pedersen, Rikke Hasle Sorensen, Dorte Launholt Lildballe, Klara Mueller, Patrick Mueller, Kira Vogel, Boris Rudic, Martin Borggrefe, Claus Oxvig, Christian Aalkjaer, Eric Schulze-Bahr, Vladimir Matchkov, Henning Bundgaard, Henrik Kjaerulf Jensen
Summary: This study investigated the prevalence of potential disease-causing variants in SQTS patients, with a focus on the SLC4A3 gene. Results showed that nonsynonymous variants in SLC4A3 were the most common cause of SQTS, highlighting the importance of including SLC4A3 in the genetic screening of SQTS or sudden cardiac death patients.
Article
Nanoscience & Nanotechnology
Ewan K. S. McRae, Helena Ostergaard Rasmussen, Jianfang Liu, Andreas Boggild, Michael T. A. Nguyen, Nestor Sampedro Vallina, Thomas Boesen, Jan Skov Pedersen, Gang Ren, Cody Geary, Ebbe Sloth Andersen
Summary: RNA origami is a method for designing RNA nanostructures that can self-assemble through co-transcriptional folding. This study uses cryogenic electron microscopy to determine the structure of RNA origami sheets and bundles, revealing structural parameters and folding principles. The study also combines sheets and bundles to construct a multidomain satellite shape and characterizes its flexibility using cryo-electron tomography.
NATURE NANOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Soren K. Amstrup, Sui Ching Ong, Nicholas Sofos, Jesper L. Karlsen, Ragnhild B. Skjerning, Thomas Boesen, Jan J. Enghild, Bjarne Hove-Jensen, Ditlev E. Brodersen
Summary: Using cryogenic electron microscopy, researchers discovered that PhnJ mediates the binding of the ATP-binding cassette proteins PhnK and PhnL to the core complex, and ATP hydrolysis leads to structural rearrangement and opening of the complex. This study sheds light on the breakdown of phosphonate in bacteria and provides insights into the mechanism of the large carbon-phosphorus lyase enzyme.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Christian E. Rusbjerg-Weberskov, Mette Liere Johansen, Jan S. Nowak, Daniel E. Otzen, Jan Skov Pedersen, Jan J. Enghild, Nadia Sukusu Nielsen
Summary: Human periostin is a matricellular protein that plays important roles in extracellular matrix remodeling, tumor development, and inflammatory diseases. The C-terminal domain of periostin interacts with other domains and proteins, potentially contributing to the pathogenesis of diseases such as atopic dermatitis.
Article
Biochemistry & Molecular Biology
Mohammad Hossein Asgarshamsi, Mehrdad Mohammadpour Dehkordi, Hossein Mohammad Beigi, Afshin Fassihi, Lotfollah Saghaie
Summary: Novel inhibitors based on benzo[d]thiazol-2-yl)-1phenylmethanimine derivatives were designed to reduce aggregation in Alzheimer’s disease. The biological evaluation showed compound 5a and 7e as the most effective inhibitors of Aβ aggregation.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Biochemical Research Methods
Rasmus Hejlesen, Kasper Kjaer-Sorensen, Angela Fago, Claus Oxvig
Summary: By using the CRISPR/Cas system, three distinct zebrafish mb knockout lines were generated, all of which did not exhibit significant phenotypic changes during development. The validated knockout line, mb(Auzf13.2), showed no signs of genetic compensation or off-target effects in adult zebrafish, suggesting promise for investigating the biological role of Mb.
TRANSGENIC RESEARCH
(2023)
Article
Multidisciplinary Sciences
Anne Skakkebaek, Kasper Kjaer-Sorensen, Vladimir V. Matchkov, Lise-Lotte Christensen, Jesper Just, Cagla Comert, Niels Holmark Andersen, Claus Oxvig, Claus Hojbjerg Gravholt
Summary: The genetic basis of the QT interval, which represents the time for heartbeat repolarization, is not fully understood. This study explores the contribution of the pseudoautosomal gene SLC25A6 to QT interval variation using human participants and zebrafish models. The results show that higher expression of SLC25A6 is associated with shorter QTc intervals, and pharmacological modulation of K-ATP channels can restore the interval duration. This indicates that SLC25A6 plays a role in QT interval variation.
SCIENTIFIC REPORTS
(2023)
Article
Optics
Anisha Chirumamilla, Maria H. Salazar, Deyong Wang, Peter K. Kristensen, Duncan S. Sutherland, Manohar Chirumamilla, Vladimir N. Popok, Kjeld Pedersen
Summary: Various nanostructures capable of generating strong local electromagnetic fields have been extensively studied for plasmonic applications. In this research, pin-shaped gold nanodiscs were fabricated on silicon posts to decouple the optical near fields from the substrate. The optimal diameter of the silicon post was achieved through reactive-ion etching, resulting in a 3-fold increase in the electric field enhancement compared to similar structures on bulk silicon. The pin-shaped nanostructures showed a significant increase in the enhancement factor in SERS measurements. This work suggests a method of engineering 3D morphology to tune the substrate influence on plasmonic properties and develop efficient nanofabrication technologies.
Article
Chemistry, Physical
Mikkel Madsen, Hossein Mohammad-Beigi, Peter Westh, Finn L. Aachmann, Birte Svensson
Summary: The complex coacervation between alginate and beta-lactoglobulin can be controlled by pH and temperature, making it suitable for different purposes. Detailed characterization of the coacervation process and particles provides insights into molecular interactions and the effects of external factors. These findings have implications for microencapsulation and drug delivery.