Review
Cell Biology
Mohadeseh Nemati, Bipin Singh, Rakeeb Ahmad Mir, Mahdieh Nemati, Azadeh Babaei, Mahdi Ahmadi, Yousef Rasmi, Afsaneh Gholinejad Golezani, Jafar Rezaie
Summary: Plant-derived extracellular vesicles (P-EVs) have multiple functions in plant physiology and can be isolated from various plant species. P-EVs have potential applications in cell-free therapy and drug-delivery systems, but clinical translation faces challenges.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Biochemistry & Molecular Biology
Jin Qian, Dong Jun Park, Sophia Perrott, Parth Patel, Brian P. Eliceiri
Summary: Extracellular vesicles (EVs) play a crucial role in intercellular signaling in inflammation and wound healing kinetics, but understanding the mechanisms behind these responses is still limited. Using cutaneous injury models and sterile subcutaneous polyvinylalcohol (PVA) sponges, researchers have been able to study the biology of EVs without the need for tissue dissociation, identifying factors that affect EV biogenesis and establishing a foundation for analyzing immune cells and EVs in different conditions such as immunodeficiency and obesity. Additionally, novel technologies like vesicle flow cytometry (vFC) have allowed for quantifying changes in EVs and studying the heterogeneity of intercellular communication in wound beds.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Urszula Szwedowicz, Zofia Lapinska, Agnieszka Gajewska-Naryniecka, Anna Choromanska
Summary: Until 30 years ago, extracellular vesicles (EVs) were believed to be used for removing unnecessary compounds from cells. However, their enormous potential for diagnosing and treating diseases has now been recognized. EVs play a crucial role in intercellular communication by transferring bioactive molecules between cells. Compared to lab-created drug nanocarriers, EVs are more stable in physiological conditions and have lower immunogenicity and cytotoxicity. EVs can deliver cargo to specific cells due to their membrane proteins and lipids. New strategies have been developed to create tailor-made therapeutic EVs. However, there are significant challenges in applying EVs as drug-delivery agents in clinical settings.
Article
Biology
Dmitry Ter-Ovanesyan, Tal Gilboa, Bogdan Budnik, Adele Nikitina, Sara Whiteman, Roey Lazarovits, Wendy Trieu, David Kalish, George M. Church, David R. Walt, Marcio L. Rodrigues
Summary: Extracellular vesicles (EVs) are challenging to separate from abundant free proteins and lipoproteins. We developed a digital ELISA assay to measure ApoB-100, a protein component of lipoproteins, and combined it with other assays to quantify the separation of EVs from lipoproteins and free proteins. We compared EV separation using different pore sizes in size exclusion chromatography and developed improved methods for EV isolation using multiple types of chromatography resins. Our approach allows quantitative measurement of impurities in EV isolation and enables novel methods for enriching EVs from human plasma.
Article
Chemistry, Analytical
Sedighe Khaksari, Khalil Abnous, Farzin Hadizadeh, Mohammad Ramezani, Seyed Mohammad Taghdisi, Seyed Ali Mousavi Shaegh
Summary: Extracellular vesicles (EVs) are membrane-enclosed vesicles secreted from mammalian cells, which act as multi-component delivery vehicles for intercellular communications. Probing circulating EVs has great potential for early diagnostics of diseases, and biosensors have attracted attention in the identification of EVs due to their simplicity and portability. Amplification strategies, including various detection modalities and microfluidic systems, are essential for detecting the extremely low concentrations of EVs in bodily fluids at early stages of a disease.
Review
Cell Biology
Lana Ginini, Salem Billan, Eran Fridman, Ziv Gil
Summary: Extracellular vesicles (EVs) are crucial heterogenous lipid structures secreted by cells for cell-to-cell communication. EVs primarily enter cells through endocytosis, a process that may depend on proteins and lipids on the surfaces of both vesicles and target cells.
Review
Endocrinology & Metabolism
Tingting Chen, Fang Wang, Jiayi Wei, Le Feng
Summary: Extracellular vesicles (EVs) are secreted by almost all cell types and present in almost all biological fluids. They have been found to have potential roles in the diagnosis and treatment of diabetic retinopathy (DR). Changes in EVs' quantity and content can function as biomarkers for the pathological status of DR, such as inflammation, neovascularization, and epithelial-mesenchymal transition. In addition, as natural, safe, and efficient drug carriers, EVs play crucial roles in intercellular communication by delivering essential cell-specific information to target cells. This review summarizes the roles of EVs secreted by various cell types and involved in various biological processes in the pathogenesis, diagnosis, and treatment of DR.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biology
Dovydas Gecys, Arunas Kazlauskas, Emilija Gecyte, Neringa Pauziene, Deimante Kulakauskiene, Indre Lukminaite, Aiste Jekabsone
Summary: This study explores the potential of genetically engineered extracellular vesicles to improve drug delivery in glioblastoma cells. The results show that introducing the RGD ligand significantly increases the internalisation of extracellular vesicles by GBM cells, leading to improved drug delivery efficacy.
Article
Chemistry, Multidisciplinary
Hang Li, Shuya Zhao, Mian Jiang, Tong Zhu, Jinjian Liu, Guoxing Feng, Lu Lu, Jiali Dong, Xin Wu, Xin Chen, Yu Zhao, Saijun Fan
Summary: This study found that exosomes derived from mice exposed to total body irradiation can protect recipient mice against radiation-induced gut injury. MiR-142, highly expressed in exosomes, plays a key role in mediating the protective effect against radiation enteritis by improving the intestinal microenvironment. Biomodification of exosomes through enhancing miR-142 expression and intestinal specificity can further improve the protection against radiation enteritis.
Article
Biochemical Research Methods
Zivile Useckaite, Lauren A. Newman, Ashley M. Hopkins, Sonja Klebe, Alex D. Colella, Nusha Chegeni, Ruth Williams, Michael J. Sorich, A. David Rodrigues, Tim K. Chataway, Andrew Rowland
Summary: The study aimed to compare the proteomic profiles of extracellular vesicles (EVs) isolated from liver tissue and the extracellular space. The results showed the potential of liver-derived EVs as a liquid biopsy for understanding the functional status of the liver.
Review
Biotechnology & Applied Microbiology
Wenting Yu, Shengnan Li, Guohao Zhang, Hockin H. K. Xu, Ke Zhang, Yuxing Bai
Summary: Extracellular vesicles (EVs) are nanoparticles derived from various cells in the oral cavity, which have extensive sources and potential applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Felix Royo, Mikel Azkargorta, Jose L. Lavin, Marc Clos-Garcia, Ana R. Cortazar, Monika Gonzalez-Lopez, Laura Barcena, Hernando A. del Portillo, Maria Yanez-Mo, Antonio Marcilla, Francesc E. Borras, Hector Peinado, Isabel Guerrero, Mar Vales-Gomez, Unai Cereijo, Teresa Sardon, Ana M. Aransay, Felix Elortza, Juan M. Falcon-Perez
Summary: The study incubated 3T3-L1 mouse fibroblasts with EVs derived from MLP29 cells, leading to a general downregulation of proteins and transcripts related to proliferative and metabolic pathways. Additionally, an activation of immune system signaling was observed, indicated by the increase of ERBB2 interacting protein (ERBIN) and Cxcl2, as well as induction of ribosome biogenesis and interferon-related response molecules.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Chemistry, Medicinal
Deimante Narauskaite, Gabriele Vydmantaite, Justina Rusteikaite, Revathi Sampath, Akvile Rudaityte, Gabija Stasyte, Maria Isabel Aparicio Calvente, Aiste Jekabsone
Summary: Extracellular vesicles (EVs) play a crucial role in wound healing and potential therapeutic interventions. In addition to their natural healing capacity, EVs can be engineered to carry specific cargo for targeted pharmaceutical purposes, making them a promising treatment modality.
Review
Chemistry, Multidisciplinary
Shayna Sharma, Mostafa Kamal Masud, Yusuf Valentino Kaneti, Prarthana Rewatkar, Aayushi Koradia, Md. Shahriar A. Hossain, Yusuke Yamauchi, Amirali Popat, Carlos Salomon
Summary: EVs can serve as natural carriers for drug delivery, and their fusion with engineered nanostructures has been proposed to enhance drug loading and achieve site-specific delivery. Challenges such as isolation, heterogeneity, coexistence with biomolecules, and lack of targeted delivery have limited the potential of EVs.
Article
Biochemistry & Molecular Biology
Lorena Martin-Jaular, Nathalie Nevo, Julia P. Schessner, Mercedes Tkach, Mabel Jouve, Florent Dingli, Damarys Loew, Kenneth W. Witwer, Matias Ostrowski, Georg H. H. Borner, Clotilde Thery
Summary: This study developed a proteomic profiling approach to study EV subtype composition and identified changes in cellular protein profiles in EVs upon HIV infection. The research also found that SERINC3 plays a role in controlling the surface composition of EVs.
Article
Optics
Maddala Rachana, Sandip Swarnakar, Sabbi Vamshi Krishna, Santosh Kumar
Summary: This paper investigates a three-input AND logic gate circuit constructed using silicon material and a 2D photonic crystal T-shaped waveguide. The circuit features a small footprint, high contrast ratio, and can be applied in high-speed optical signal processing.
Article
Mathematics, Applied
Santosh Kumar, Faustine Nziku
Summary: This paper establishes and proves a fixed point theorem for Boyd and Wong type contraction in ordered partial metric spaces. The author extends several existing results into ordered complete partial metric spaces and illustrates the validity of the results through an example. Furthermore, the existence of the solution of a nonlinear integral equation is discussed as an application of the main result.
JOURNAL OF FUNCTION SPACES
(2022)
Article
Mathematics, Applied
Mohd Jubair, Javid Ali, Santosh Kumar
Summary: This paper introduces a new scheme and proves convergence results for nonexpansive mappings and weak contractions in the context of Banach spaces. It is analytically and numerically demonstrated that the proposed scheme converges to a fixed point of a weak contraction faster than existing schemes. Additionally, it is shown that the new scheme is almost stable with respect to weak contractions. Several numerical examples are provided to support the main results and the visualization is demonstrated using Matlab program. Finally, the paper approximates the solution of a nonlinear fractional differential equation by applying the main result.
JOURNAL OF FUNCTION SPACES
(2022)
Article
Cell Biology
Sonia Kiran, Ahmed Rakib, Sunitha Kodidela, Santosh Kumar, Udai P. Singh
Summary: This study explores how a high-fat diet alters the immune response in adipose tissue and influences inflammation during obesity. The findings suggest that a high-fat diet increases the levels of inflammatory cytokines and immune cells, while reducing the levels of regulatory T cells, leading to sustained adipose tissue inflammation.
Article
Chemistry, Medicinal
Lina Zhou, Sunitha Kodidela, Sandip Godse, Stacey Thomas-Gooch, Asit Kumar, Babatunde Raji, Kaining Zhi, Harry Kochat, Santosh Kumar
Summary: This article discusses the barrier of BBB to drug penetration and the use of EVs for targeted drug delivery to the CNS. Limited information on clinical trials is available, but studies on the peripheral nervous system may shed light on strategies for CNS drug delivery.
Review
Psychology, Clinical
Satish K. Kedia, Nikhil Ahuja, Patrick J. Dillon, Andrew Jones, Santosh Kumar, Sanjaya Satapathy
Summary: Alcohol use disorder is a serious public health problem, causing a significant number of deaths. This review examines the effectiveness of injectable naltrexone treatment for individuals with alcohol use disorder. The results indicate that injectable naltrexone therapy can reduce the frequency of drinking and heavy drinking days, and increase abstinence rates. However, a portion of patients still do not achieve complete abstinence after treatment.
JOURNAL OF PSYCHOACTIVE DRUGS
(2023)
Article
Engineering, Electrical & Electronic
Neha Mishra, Bramha P. Pandey, Brijesh Kumar, Vinay K. Tomar, Santosh Kumar
Summary: In this paper, the authors investigate the impact of NOx gas molecule adsorption on the sensing properties of Fe-doped MoSe2 monolayer, concluding that NO2 gas molecules are more suitably sensed by analyzing electronic properties such as adsorption energy, charge transfer, recovery time, density of states, conductivity, and sensitivity.
IEEE SENSORS JOURNAL
(2022)
Article
Mathematics
Lucas Wangwe, Santosh Kumar
Summary: This paper aims to prove fixed point results for interpolative psi-Hardy-Rogers type contraction mappings in quasi-partial b-metric spaces. We also provide an illustrative example to support our results. The results proved here will be utilized to show the existence of the solution of a nonlinear matrix equation as an application.
JOURNAL OF ANALYSIS
(2023)
Article
Engineering, Electrical & Electronic
Ajay Kumar Vyas, Rahul Kumar Gangwar, Santosh Kumar
Summary: This article proposes a sensor design based on elliptical air hole-based photonic crystal fiber (EH-PCF) for temperature and refractive index sensing. The sensor exhibits high sensitivity and can be fabricated using a simple and low-cost method, making it suitable for various applications.
OPTICAL FIBER TECHNOLOGY
(2022)
Article
Mathematics, Applied
Senthil Kumar Prakasam, Arul Joseph Gnanaprakasam, Gunaseelan Mani, Santosh Kumar
Summary: In this paper, we investigate the concept of an orthogonal L-Ψ contraction map and prove the fixed-point theorem in an orthogonal complete Branciari metric space (OCBMS). We provide illustrative examples to support our theorems. As an application of the main results, we demonstrate the existence of a unique solution to a fourth-order differential equation using a more orthogonal L-Ψ contraction operator in OCBMS.
JOURNAL OF FUNCTION SPACES
(2023)
Article
Mathematics, Applied
Santosh Kumar, Jonasi Chilongola
Summary: This paper aims to prove the existence and uniqueness of a common fixed point for a pair of ?-?-interpolative Hardy-Rogers-Suzuki-type contractions in the context of quasipartial b-metric space. Several well-known results like Hardy and Rogers, Suzuki, and others have been generalized in this work. Additionally, a demonstrative example and an application of fractional differential equations are provided to validate the findings.
JOURNAL OF FUNCTION SPACES
(2023)
Review
Medicine, Research & Experimental
Sandip Godse, Lina Zhou, Swarna Sakshi, Bhupesh Singla, Udai P. Singh, Santosh Kumar
Summary: Neurological disorders pose a global challenge and require innovative therapeutic approaches. Curcumin, a natural compound with antioxidant and anti-inflammatory properties, shows potential as an adjuvant therapy for central nervous system diseases. However, its limited bioavailability and permeability to the blood-brain barrier hinder its therapeutic efficacy. Nanocarriers can facilitate curcumin delivery, improving its bioavailability and blood-brain barrier permeability, and revolutionizing treatment outcomes for neurological disorders.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2023)
Article
Multidisciplinary Sciences
Asit Kumar, Namita Sinha, Sunitha Kodidela, Lina Zhou, Udai P. Singh, Santosh Kumar
Summary: The study found that Benzo(a)pyrene (BaP) contributes partly to macrophage and astrocyte-mediated neuroinflammation by inducing the production of IL-1 beta and MCP-1, likely involving the CYP and/or oxidative stress pathways.
Article
Computer Science, Information Systems
Abhishek Sharma, Sushank Chaudhary, Jyoteesh Malhotra, Amir Parnianifard, Santosh Kumar, Lunchakorn Wuttisittikulkij
Summary: A sensing system based on FMCW photonic radar is proposed to achieve more efficient sensing on multiple targets. The system utilizes WDM and PDM schemes for multiple target detection, and the impact of varying bandwidth on system performance is analyzed through numerical simulations.
Meeting Abstract
Psychology, Multidisciplinary
Sahar Hojjatinia, Elyse R. Daly, Timothy Hnat, Syed Monowar Hossain, Santosh Kumar, Constantino Lagoa, Inbal Billie Nahum-Shani, Shahin Alan Samiei, Bonnie Spring, David E. Conroy
ANNALS OF BEHAVIORAL MEDICINE
(2022)
